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20 1) Topley & Wilsons: Principles of Bacterio ) Huddleson, I.F.: Brucellosis in

21 man and animals, ) Wilson, G. S. & encountered in certain Br. abortus agglutinating sera, J. Path. & Bact., 34: 81-89, Miles, A. A.: The serological differentiation of smooth Straius of Brucella group, Brit. J. 28) Dean, 1-I. R.: Complement fixation - in mixtures of toxin and antitoxin. J, Path. & Bact., 30: , ) Gold Wort- N. E.: The occurence of multiple hy, zones in the serum precipitation reaction, J. Path. & Bact., 31: , ) Northop, ) Hazato, H. lida, T. & Kaneko, S.: Studies on the prozone phenomenon in antisera with special reference to the effect of cephalin and proteolytic Jap. J. Exp. Med 23: , enzyme, ) Hagato, H. lida, T. and Kazama, S.: Stn- the prozone pheuomenon dieson in antisera, Jap. J., exp. Med., 23: 313, -311, ) Hazato, H. lida, T. and Kaneko, S.: Studies on the Prozone Pheuomenon with special re frence to the modification of antisera with phenol Jap. J. exp. Med., 23: , lius, A. and Kabat, E, A.: An electrophoretic study of immune sera and Pueified antibody preparations J. Exp. Med., 23: , V. S.: The Intermadiate zone phenomenon

22 Immuno-chemical Studies on Brucella Shinji OHHIRA Department of Bacteriology, Iwate Medical College (Director: Prof. Y. TAZAWA) In the present studies, the productivity of the antibody following immunization by dead and living cells of various types of Brucella was compared with each other electropholetically and by means of the agglutinin reaction, together with experiments for immunological effects of the dead and living cells as well as some remarks about the inhibitory zone phenomenon which appeared specifically in Brucella. 1) In goat, high antibody titers were attained rapidly within a short period of time, being independent to the amount of the living and dead cells inoculated. 2) In the dead cells vaccination, a constant maintainace of the antibody was found during the course of the repeated vaccinations with the dead cells, but the antibody began to decrease with the lapse of days after a single vaccination, and was found to begin to decrease rapidly after discontinuation of the repeated vaccinations. 3) It was found that high antibody titers were found in vaccination with the living cells. 4) The inhibitory zone phenomenon was always demonstrated in case of repeated vaccinations with the dead cells, and was especially significant in case of agglutination with the antigen of the living cells. No inhibitory zone phenomenon was found in sera after discontinuation of the repeated vaccinations as well as after a single vaccination. 5) No inhibitory zone phenomenon was found to occur in vaccination with the living cells. 6) Such an inhibitory zone phenomenon is regarded as a result of hyperproduction of the antibody, and seemed to occur after the appearance of a reaction inhibiting substance which resembles the specific antibody. 7) Decrease in albumin levels as well as increase in globulin levels were found after the oneset of vaccination with the dead cells, though they recovered respectively, to the normal values, with the lapse of days after the vaccination. 8) Total protein levels do not always increase following vaccination with the dead cells, but was found to increase following vaccination with the living cells. 9) No change in al-a2 globulin levels was found following vaccination with the dead or living cells. 10) Parallel increase of 0-globulin and 7-globulin levels was found following vaccination with the dead cells, both levels having begun to decrease or return to the normal values after discontinuation of the vaccination. 11) Parallel increase of /3-globulin and 7-globulin levels was found following vaccination with the living cells, and a constant high level in both kinds of globulin was maintained for a long period. 12) No immunological effects were found in case of vaccination with the dead cells. This fact seems to result from that the 7-globulin levels tended to return to the normal value within a short period after the vaccination.

semen quality or those without WBC in semen. In the patients with azoospermia and normal FSH levels (normogonadotropic azzospermia), the antibody (IgG

semen quality or those without WBC in semen. In the patients with azoospermia and normal FSH levels (normogonadotropic azzospermia), the antibody (IgG CLINICAL STUDIES OF UROGENITAL INFECTIONS WITH CHLAMYDIA TRACHOMA TIS Report 2. The Epidemiology of Chlamydial Infections in Okayama District in Japan and Detection of Antibodies to Chlamydiae in the Sera

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3) Stedmann, E. et al.: Biochem. J., 29; 2107, 1935. 4) Reiss, M.. Hemphill, R. E. E ENature, 161 ; 18, 1948. 5) Tower, D. B. & D. MeEachern: Canada J. Reserch., 27; 105, 120, 132, 1949. 6) Wolleman, M.

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