Takio ICHITANI and Teruko HIGASHIKAWA: Germination of Aphanomyces iridis oospores on the basal medium for selective isolation with addition of bacteri

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1 Takio ICHITANI and Teruko HIGASHIKAWA: Germination of Aphanomyces iridis oospores on the basal medium for selective isolation with addition of bactericides and fungicides Summary This study examined the effect of different bactericides and fungicides on oospore germination of Aphanomyces iridis. The results obtained are as follows. (1) Oospores can be divided into phologically complete and incomplete oospores under a light microscope. (2) Both corn meal decoction (CMD) and FOWLES's defined liquid (FDL) media are suitable for producing many complete oospores. (3) Complete oospores with high germinability can be obtained after growing the fungus on either CMD or FDL medium for 2 weeks at 25 Ž. (4) Optimum temperature and ph for oospore germination are and about 6.4, respectively. (5) No inhibitory action of oospore germination is usually seen when chloramphenicol, streptomycin sulfate and vancomycin are used at concentrations up to 100, 80 and 400 ppm, respectively, although the latter two are both sometimes inhibitory at and 100 ppm.(6) Anphotericin B, benomyl and iprodione inhibit oospore germination at concetrations higher than 5, 0.5 and ppm, respectively. Benomyl is highly inhibitory even at ppm. Metalaxyl inhibits at the intermediate concentrations used and thiophanatemethyl inhibits at the concentrations higher than ppm. Proc. Kansai P1. Prot. Soc. (28):19-24(1986) College of Agriculture, University of Osaka Prefecture, Sakai 591, Japan.

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3 Fig.1. Relation between production of morphologically complete oospores and their germination. (- E-:Dry weight, - -: No. of incomplete oospores produced, - -: No. of complete oospores produced, - œ-: Germination of complete oospores)

4 Table 1. Effect of different bactericides on oospore germination of Aphanomyces iridis (UOP Ap-37) on Bacto-corn meal (1) Means within a column not followed by the same letter are significantly different (P:0.05 by t Table 2. Effect of different fungicides on oospore germination of Aphanomyces iridis (UOP Ap-37) on Bacto-corn meal agar (1) Means within a column not followed by the same letter are significantly different (P:0.05 by t \ \: Not conducted.

5 3) FOWLES, B.(1976) Mycologia 68: ) GHAFOOR, A.(1964) Phytopathology 54:1167 1) BOOSALIS, M.G. and SCHAREN, A.L. (1959) Phytopathology 49: ) IKEDA, A. and ICHITANI, T.(1985) Bull. Univ. Osaka Pref., Ser. B., 37: ) KUSUNOKI, M. and ICHITANI, T.(1982) Ann. Phytopath. Soc. Japan 48: ) MILLER, P.M. (1955) Phytopathology 45:461 14) PAPAVIZAS, G.C. and AYERS, W.A. (1974) USDA Tech. Bull pp. 15) PFENDER, W.F., DELWICHE, P.A., GRAU, C.R. and HAGEDORN, D.J. (1984) Plant Disease 68 :

6 24 関 西 病 虫害 研 究 会報(28)1986 Plate I 1 `5: Morphologically -normal complete oospheres)6: oospores(thick y unrecognizable (Thick arrows-2 arrows -clearly observed walls; thin arrows Morphologicall walls; germ thin tubes). arrows-granular Bars incomplete or on the plate oospores almost empty represent (Thick oospheres 10ƒÊm. arrows-wavy 7: Germinating or almost oospore

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