TissueGnostics HistoFAXS TissueGnostics HistoFAXS HistoFAXS 最新の技術に裏づけられた細胞解析システム HistoFAXS HistoFAXS HistoFAXS HistoFAXS Zeiss 2 HistoFAXS HistoQuest
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2 HistoFAXS 最新の技術に裏づけられた細胞解析システム HistoFAXS HistoFAXS HistoFAXS HistoFAXS Zeiss 2 HistoFAXS HistoFAXS 200ROI FOV Forward Connection Backward Connection/Gating mm 2 mean value
3 HistoFAXS ワークフロー 100x
4 HistoFAXS 組織画像取得および画像データマネージメントソフトウェア HistoFAXS 8 ROI TMA
5 HistoFAXS TMA ワークフローと特徴 TMA TMAHistoFAXS HistoFAXS TMA TMA Acquired and imported spot image TMA HistoFAXS TMA TMA ID TMA ID Excel TMA explorer report generator TMA Logical groups logical group
6 プロジェクトと画像の読み込み HistoFAXS ROI HistoFAXS HistoFAXS ROI All images courtesy of O.Univ.Prof. Dr.med.Helga Fritsch and Elisabeth Richter, Medical University Innsbruck, Division for clinical functional Anatomy,
7 カラー分解 Hemalaun RGB Gain, Sigma, Gauss TissueGnostics Cyclin A Cyclin A Gain, Sigma, Gauss Cyclin A Images courtesy of Prof. Dr. Ingela Turesson, University Hospital Department of Oncology, Uppsala, Sweden Prof. Dr. Anna Danielsson, Sahlgrenska Sjukhuset - University Hospital, Department of Oncology, Gothenburg, Sweden 12 13
8 細胞核の識別 TissueGnostics 14 15
9 細胞質の識別 取得したデータ 2 Hemalaun vs. Cycline A 3,9% Cyclin A + cells Forward Connection Backward Connection/Gating 9 Quadrant Hemalaun Mean All events in scattergram Cyclin A Count Percent No./mm Mean UL 0,000 0, ,00% 0,000 UR 124,815 92, ,90% 4 LL 42,249 0, ,24% 1624 LR 114,104 1, ,86% 1694 Overall 115,104 4, % 63 Histogram 16 17
10 Forward Connection と HistoFAXS Compare Set Compare Set Backward Connection/Gating Backward/Forward Connection Forward Connection Backward Gating ROI Cyclin A vs Cyclin A Cyclin A vs Cyclin A Forward Connection Backward Connection 18 19
11 による解析結果と目視による計数の比較 Albert-Ludwings University Freiburg Institute of Molecular Medicine Reinheckel Group 14 MMTV-PyMT Ki67 乳がん Ki67 + vs Ki67 - Ki67 HER2 35,64% of Cells are Ki ,36% of cells are Ki67 - All images and graphs on this page courtesy of Dr. Thomas Reinheckel, Albert-Ludwigs University Freiburg, 2007 n=10 estrogen progesteron All images courtesy of Univ. Doz. Dr. Johann Feichtinger, Pathology-Bacteriological Institute, Rudolfstiftung Hospital, Vienna 20 21
12 PSA 発現と HDAC1 発現 Ludwig Boltzmann Institute for Cancer Research LBI-CR Prof. Dr. Lukas KennerHisto Quest PCPSA Prostate Specific Antigen HDAC1 Histone Deacetylase PC PSA Normal PIN CA Ao. Univ. Prof. Dr. Lukeas Kenner, Clinical Institute of Pathology Medical University Vienna PC HDAC1 Normal PIN CA PC PSA Androgen Normal PIN CA All images and graphics courtesy Ao. Univ. Prof. Dr. Lukas Kenner, Clinical Institute of Pathology, Medical University Vienna PC HDAC1 Androgen Normal PIN CA All images and graphics courtesy Ao. Univ. Prof. Dr. Lukas Kenner, Clinical Institute of Pathology, Medical University Vienna 22 23
13 特徴 HistoFAXS.tif.jpg Forward Connection Backward Connection/Gating 24 25
14 技術仕様 Zeiss AxioImager. Z1 Zeiss AxioObserver. Z1 7 1x 100x Intel Core 2 Quad, 4 GigaB RAM, 1 TeraB HDD, Windows XP 24 TFT nm ( n) 1 m 4 m 1.9 megapixel 1600x fps VGA cm x 80 cm 100 VAC 10A LED Diascopic illumination 12V-100W 26 27
15 TissueGnostics GmbH Taborstrasse 10/2/8 A-1020 Wien, Austria Tel.: Fax: office@tissuegnostics.com TissueGnostics USA Ltd. 420 N Larchmont Boulevard CA 90004, Los Angeles, USA Tel.: Fax: office@tissuegnostics-usa.com TissueGnostics Romania SRL Nicolae Iorga Boulevard nr. 51C Iasi, Romania Tel.: Fax: office@tissuegnostics.ro TEL FAX URL
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