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- しげじろう ねぎたや
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3 217/ AH1pdm9 AH3 B HI AH1pdm9 B AH3 D151GD151N HA 6 AH1pdm9 AH3 B /18-1 -
4 well MDCKLLC-MK2HEp-2HeLa RD-18sVero Caco-2 217/18 3 1).75% HI A(H1N1)pdm9 B 3 A(H3N2) 2 QIAamp Viral RNA Mini Kit QIAGEN RNA OneStep RT-PCR Kit QIAGEN NA RT-PCR PCR QIAQuick PCR Purification Kit QIAGEN DNA BigDye Terminator v3.1 Cycle Sequencing Kit Applied BiosystemsBigDye XTerminator KitApplied BiosystemsApplied Biosystems 35 MEGA6 NJ AH1pdm9 NA H275Y 1) AH3 NA E119VD151VQ226HG248RK249ER292KN294S 1,3,4) B NA R15KD197ED197NI221TN294SG47S 1) - 2 -
5 217/ ) 1 2 6) / AH1pdm %AH %B % B 1 1.5% 3-3 -
6 B Yamagata 36.8% B Victoria 1.5% A H1pdm9 27.9% A H3 33.8% 2 217/ B Victria B Yamagata AH3 AH1pdm /18 AH1pdm9 AH3 B B 25:1 3:1 7) - 4 -
7 AH3 D151GD151N NA 8) HI AH3 23 A/Hong Kong/481/214 HI % 7) 3 1 AH1pdm B AH1pdm9 A/Bangladesh/25/217 A/Philipp ines/5/2 A/Singapore/GP198/ /19 WHO A/Michigan/45/215 9) 5 AH3 216/17 A/Hong Kong/481/ /19 A/Singapore/INFIMH-16-19/216 6 B 218/19 B/Maryland/15/
8 A(H1N1)pdm9 NA A(H3N2) NA B NA /18-6 -
9 7% 1) :, 3, ),,,:,6,61-66,29 3) Monto,A.S.,McKimm-Berschkin,et al.:antimicrobial Agents and Chemotherapy.,5, ,26 4) Sheu,T.G.,Deyde,et al.:antimicrobial Agents and Chmotherapy.,52, ,28. 5) : l ) ; nsenshou1/houdou.html ),;217/ ) Yi Pu Lin. et al. Neuraminidase Receptor Binding Variants of Human Influenza A(H3N2) Viruses Resulting from Substitution of Aspartic Acid 151 in the Catalytic Site: a Role in Virus Attachment?.Journal of Virology. 21,vol. 84, no. 13, p ( ). 9) WHO;Recommended composition of influenza virus vaccines for use in the northern hemisphere influenza season tion.pdf
10 - 8 -
11 μl μl μl - 9 -
12 G G G G - 1 -
13 - 11 -
14 - 12 -
15 - 13 -
16 AA AA AA AA AA 1) 28 AA LC-MS/MS 2) AA AA 1 mg/ml AA1,2,3-13 C31 mg/ml Waters Sep-Pak AC-24 mgsep-pak C181 g 1 LCWaters Acquity UPLC H-class MSWaters Xevo TQD Waters:HSS C181.8µm 2.1 1mm µL A B.4mL/min 6.5 Time(min) A B ESI+ 1.5kV 12 L/hr
17 MRM m/z CV(V) CE(eV) C ) AA AA AA 1 AA AA 4 AA C18 AC-2 AA 1 2 AA AA 2 AA AA
18 15 ng/g N.D. 15 ng/g N.D cm 2 ng/g W ng/g N.D. N.D ng/g W 5g ng/g ng/g ng/g
19 3 ng/g N.D. 1 5W ng/g N.D ng/g N.D ng/g N.D ng/g ng/g N.D ng/g
20 ng/g AA AA AA cm ng/g N.D. N.D. N.D. N.D ng/g
21 ng/g ng/g ng/g W 5g ng/g ng/g N.D. N.D ng/g ng/g ng/g
22 AA 4 AA AA ng/g AA AA AA 1)IARC 2) 28 3) - 2 -
23 B1 B1B2G1G HPLC LC/MS LC-MS/MS (1) B1B2G1G2 25µg/mL LC/MS Inert Sep VRA-3GL (2) (3) 1 µg/kg 4 µg/kgn=1 a. 5 2 b
24 5. 9:1 2 ml 3mm 1) 3mm 3 33 rpm 1 4 ml (4) (3) a. 2.2 ml 2. ml 45 (5) 1. ml LC-MS/MS 3:2 1 1 (6) LC ACQUITY UPLC-H Class MS Xevo TQD Waters 1-1 LC MS-Tune Waters:BEH C18 1.7µm 2.11 mm 4 A5mmol/L B5mmol/L.3 ml/min 5 µl Time(min) A B MS-Tune ESI+.75kV 1L/hr 55 33L/hr MRM Cone CE RT ESI (V) (ev) B > > B > > G > > G > > (1)
25 .5 ng/ml 1 IDL3IQL ng/ml 1. µg/kg.5 ng/ml.251 ppb R B1 B2 G1 G2.5 ng/ml ( ng/ml ) IDL( ng/ml ) IQL( ng/ml ) R ng/ml (2) a B1 B2 G1 G b. 4 4 B1 B2 G1 G mm
26 (3) (2) 2.5 µg/kg 1µg/kg B1 B2 G1 G Inert Sep VRA-3 LC-MS/MS 1) p
27 LC-MS/MS ) α- β- 3 HPLC LC-MS/MS α- β- (1) 3 (2) α-β- 2 LC/MS (1) (2)
28 (3) 1 1 (µg / g) α.2 β.2 ND ND ND (4) (2) (3).2 µg/g ) ml 3,rpm1 1 ml (5) LC ACQUITY UPLC-H Class MS Xevo TQD Waters 2-1 LC Waters:BEH C18 1.7µm mm 5 A.5 B C5mmol/L.4 ml/min 1 µl Posi Time(min) A B Nega Time(min) C B ml Oasis HLB 4.4 ml 1.6 ml 2. ml Filter.2 µm LC/MS/MS 1 ml 1 ml 1 ml 1 ml
29 2-2 MS-Tune ESI().751. kv 12L/hr 65 33L/hr MRM ESI m/z Cone CE RT (V) (ev) α β (1) 3.2 ng/ml 2 ng/ml 1 IDL3IQL ( ng/ml ) ( ng/ml ) IDL ( ng/ml ) IQL ( ng/ml ) () R 2 ng/ml (.51.).2 ng/ml 2 ng/ml N=1.2 µg/g.25 ng/ml.5 ng/ml 1. ng/ml1 ng/ml1 ng/ml R2.999 (2) 4.2 µg/g 5.1 µg/g.2 µg/g S/N
30 4.1 µg/g 1.2 µg/g 2 α β ng/ml2.625 ng/ml 2.25 ng/ml 3 S/N 1 (3) 5 LC-M/SMS ME STD2 STD111 STD1 2 STD2 5 ME ME α- β- ng/ml α- S/N 1 (4) 6 (1)(3) α- 2.2 ppm β
31 HPLC 4 α-β- LC-MS/MS 3 β
32 LC-MS/MS TTX TTX LC-MS/MS TTX 1 TTX LC-MS/MS 1 ) 2 ) LC-MS/MS (1) TTX 1mg 1mL LC/MS (2) JIS (3) 7 HLB (2mg)PRiME HLB (2mg) ODS (1g) AmiconUltra-4 centrifugal filter Device 4mL 1K NMWL) 1 ODS(36mg) GC5mg 2AC2-3 -
33 1 MILLIPORE 2 GL Waters (4) Waters Xevo TQD LC ACQUITY UPLC-H Class LC-MS/MS WatersACQUITY UPLC BEH HILIC 1.7 µm mm A.5 B.2 ml/min 5 µl A B MS Tune ESI.5 kv 1L/ hr MRM ESI Cone(V) CE(eV) RT TTX + 32> > (1) TTX.5 ng/ml > ng/ml > TTX 1-1 TTX (.21 ng/ml) 1-2 TTX1ng/mL.443 ng/ml
34 2.2µg/g.8 ng/ml 32>32 2 TTX ng/ml ng/ml IDL (3σ) IQL (1σ) (2) 2-1 TTX 1 MU/g 2.2 µg/g 1 2 µg/g 2 µg/g 1 3 TTX.5 ng/ml % 25 ml 1 3,rpm ml 2 % 1 ml 2mL 2 % 5 ml 5 ml 4 ml.2 µm LC-MS/MS HLB ODS ACNMeOH75151 PRiME HLB AmiconUltra-4 3 TTX µg/g 2 2 PRiME-HLB PRiME-HLB PRiME-HLB HLB ODS
35 (3) 1 ng/ml STD1 1 ng/ml STD2 ME STD2 STD % 4 1 ng/ml 2-2 GCB AC2 2 GCB AC2 5 % g/ml 1 g/ml ME % ml ODS GCB GCB 5 ml1 ml AC2 3 ml 2EtOH 14mL 5 TTX 5 GCB AC ACNMeOH:H 2 O7515:1) 1 ml.2 µm LC-MS/MS 2-2 LC-MS/MS TTX HILIC.21 ng/ml R=.999 TTX
36 2 ng/ml 1) LC-MS/MS TTX 48 2) LC/MS/MS
37 1-35 -
38 11/7-11/3 11/3-12/26 12/26-1/19 1/19-2/8 2/8-3/5 (23 ) (26 ) (24 ) (2 ) (25 ) (25) 6.5km (1) 6km (1) 15km (1) 1km 12 2km
39 cm 2,12.5cm8cm1ml 5ml 2rpm 1min.2m ICS-11μg/cm 2 /
40 3-38 -
41 - 39 -
42 - 4 -
43 ) H nss-so4 2 nssnon-sea salt m 15m 1.7km
44 US cm ) 1 1 ph EC SO4 2 NO3 Cl NH4 Na K Ca 2 Mg 2 ph EC ph EC 1 ph ph 5.6 EC mS/m phec 1 3 EC [ms/m] ph ph EC H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29 1 ph EC
45 (a) 2(b) 2(a) NH4 NO3 Na Cl Na Cl 4 NO3 nss-so4 2 2(b) NO3 nss-so nss-so4 2 NH4 NO3 nss-so ph [%] H29 H28 5 H (a) [μeq/l] H29 H28 5 H28 5 Na+ Cl- Mg2+ ss-so42- K+ nss-so42- ss-ca2+ NO3- nss-ca2+ NH4+ H+ nss-ca2+ NH4+ H+ nss-so42- NO (b) 2 R1R R1 R2 3 R
46 R1 R2 R1 [%] H28 H Cations + Anions [μeq/l] R2 [%] 3 2 H28 H EC [ms/m] 3 R1 R2 EC EC Na nss-ca nss-ca 2 NO3 nss-so4 2 NH ) H NO nss-so4 2 H 4) ) 1mm mm115mm mm Na 1 EC
47 NO3 NH EC [mm] [ms/m] nss-ca Na+ [μmol/l] [μmol/l] [μmol/l] [μmol/l] NO nss-so NH4+ 7 H+ [μmol/l] [μmol/l] mm 25 [day] H28 H29 H23H27 H23H27 H23H
48 [mm] [ueq/l] [ueq/l] [ueq/l] [ueq/l] [ueq/l] [ueq/l] H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29 nss-ca2+ Na+ H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29 NO3- H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29 NH4+ H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29 nss-so42- H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29 H+ H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29 5 nss-so4 2 NO3 22 NO3 H nss-so ueq/l ueq/l Ca 2 NO3 NH4 nss-so4 2 ph ph
49 NH I: μeq/l NH : NH μeq/l 6 nss-so4 2 H nss-so4 2 H Ca 2 NO3 NH4 H nss-so nss-so42- H+ NO3- nss-ca nss-so42- H+ NO3- nss-ca2+ -1 H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29-1 H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29 6 NH ph EC ph EC Na Cl NO3 nss-so4 2 EC Na NO3 nss-so4 2 NH4 H NO3 nss-so4 2 1mm
50 Na NO3 NH4 NO3 NH4 nss-so4 2 NO3 22 NO3 H nss-so ph NH4 Ca 2 nss-so4 2 1) a.files/h28nenpo_kenkyuu.pdf 2) 2 21https:// 3) ) )
51 ASPT 2 2 Shannon ASPT (1) (2) 1 St- 1 St- 2 St- 3 St St- 1 St- 2 St- 3
52 St-3 St-3 St-2 St-4 St-1 St-1 St-2 (3) 2 ph (4) 2 Shannon ASPT ASPT ASPT ASPT
53 ASPT ASPT ASPT ASPT ASPT =() 33 4 Shannon Shannon Weaver1963 () H n N S N nii 3 (1) 2 3 (a)(b) Shannon 4-1 St- 1 3m 2 1 /s ASPT Shannon St- 2 5m 4 5 /s ASPT 7.1 St Shannon 1.9 St ln n N
54 -3 St- 3 BOD75 7m St- 1 9ASPT 4. St- 1 St- 2 St- 1 Shannon 2.2 St- 1 St- 2 ASPT Shannon -4 St- 4 25m ASPT 3.9 St Shannon
55 a
56 b
57 Shannon
58 ph Shannon SS St- 1 St- 2St ASPT St St St St ASPT Shannon St- 1 St- 3 2 St Shannon
59 BOD75% ASPT 2 BOD75 7 ASPT St- 1 St BOD75% St- 4 ASPT St- 1 8 St St- 3 7m St- 4 Shannon ASPT BOD75% ASPT (a) St- 1 (b) St- 2 (c) St- 3 (d) St- 4 2 BOD75% ASPT (2) 6 7 (a)(b) 8 ASPT Shannon -1 St- 1 4m 8 16 /s m
60 9 7 3 ASPT 6.3 ASPT 5.7 Shannon.71-2 St- 2 5m 7 12 /s ASPT 6. ASPT 5.4 Shannon 1.5 St- 1-3 St- 3 8m 7 1 /s ASPT ASPT 4 1 ASPT Shannon
61 7 (a) (b)
62 8 ASPT Shannon 9 ASPT ASPT ASPT ASPT Shannon 9 3 ph ASPT St St
63 St St- 1 St- 2 ASPT ASPT St ASPT ASPT ASPT St- 1 St- 2 St- 3 3 St- 3 St- 1 St- 2 St- 3 Shannon St Shannon
64 BOD75 ASPT 3 BOD75ASPT ASPT St- 1 St- 3 BOD75 St- 1St- 3 1 BOD75ASPT St- 1 St- 2 6 St ASPT BOD75 ASPT St- 2 St- 3 ASPT St- 1 (a)st- 1 1 (b)st (c)st ASPT BOD75 4 (1) ASPT St St St St ASPT Shannon St- 1 St- 3 2 St
65 (2) ASPT St St St St- 1 3.St St Shannon St St- 1St- 3 1 BOD St St St (3) ASPT St St St (2) ASPT ASPT ASPT ASPT St- 1 3 St- 1 St- 2 2 ASPT ASPT
66
67 - 65 -
68 - 66 -
69
70 - 68 -
71 - 69 -
72 - 7 -
73 - 71 -
74 No.6 No.1 COD a COD a a COD COD No.6 No.1 1) COD ) No.6 No.7No.1 No.2 m-3m+1m
75 No.2 No.3 No.1 No.1 No.3 No.4 No.6 No.7 No.1 A B 1 1) 1 1 No.6 ィ a C O D C O D No.7 No.1 No
76 No.2 8 COD Chl.a Cl [] No.6 No.7 No.1 No COD [mg/l] No.6 No.7 No.1 No COD Cl [mg/l] No.6 No.7 No.1 No Cl [mg/l] Cl
77 Chl.a [mg/l] No.6 No.7 No.1 No Chl.a T-N [mg/l] No.6 No.7 No.1 No T-N T-P [mg/l] No.6 No.7 No.1 No T-P
78 COD COD 75% A COD75% 2 No.1No.3 No.4 COD No.6 No.1 COD 5. A COD 75% [mg/l] No.1 No.3 No.4 No.6 No.1 (2mg/L) 2 A COD 75% B COD75% 3 No.7 B COD COD 75% [mg/l] B No.7 (3mg/L) 3 B COD 75% A COD75% 3 No.1No
79 No.3 COD 5. A COD 75% [mg/l] No.1 No.3 No.2 (2mg/L) 4 A COD 75% COD 1 COD 75% 5% % COD 1 5 No.1No.3No.4No.6 No.7 No No.1No.2 No No.6 No.1 75%
80 COD '89-'98 '9-'99 '91-' '92-'1 '93-'2 '94-'3 '95-'4 '96-'5 '97-'6 '98-'7 '99-'8 '-'9 '1-'1 '2-'11 '3-'12 '4-'13 '5-'14 '6-'15 '7-'16 '8-'17 COD [mg/l] No '89-'98 '9-'99 '91-' '92-'1 '93-'2 '94-'3 '95-'4 '96-'5 '97-'6 '98-'7 '99-'8 '-'9 '1-'1 '2-'11 '3-'12 '4-'13 '5-'14 '6-'15 '7-'16 '8-'17 COD [mg/l] No '89-'98 '9-'99 '91-' '92-'1 '93-'2 '94-'3 '95-'4 '96-'5 '97-'6 '98-'7 '99-'8 '-'9 '1-'1 '2-'11 '3-'12 '4-'13 '5-'14 '6-'15 '7-'16 '8-'17 COD [mg/l] No '89-'98 '9-'99 '91-' '92-'1 '93-'2 '94-'3 '95-'4 '96-'5 '97-'6 '98-'7 '99-'8 '-'9 '1-'1 '2-'11 '3-'12 '4-'13 '5-'14 '6-'15 '7-'16 '8-'17 COD [mg/l] No '89-'98 '9-'99 '91-' '92-'1 '93-'2 '94-'3 '95-'4 '96-'5 '97-'6 '98-'7 '99-'8 '-'9 '1-'1 '2-'11 '3-'12 '4-'13 '5-'14 '6-'15 '7-'16 '8-'17 COD [mg/l] No '89-'98 '9-'99 '91-' '92-'1 '93-'2 '94-'3 '95-'4 '96-'5 '97-'6 '98-'7 '99-'8 '-'9 '1-'1 '2-'11 '3-'12 '4-'13 '5-'14 '6-'15 '7-'16 '8-'17 COD [mg/l] No '89-'98 '9-'99 '91-' '92-'1 '93-'2 '94-'3 '95-'4 '96-'5 '97-'6 '98-'7 '99-'8 '-'9 '1-'1 '2-'11 '3-'12 '4-'13 '5-'14 '6-'15 '7-'16 '8-'17 COD [mg/l] No '89-'98 '9-'99 '91-' '92-'1 '93-'2 '94-'3 '95-'4 '96-'5 '97-'6 '98-'7 '99-'8 '-'9 '1-'1 '2-'11 '3-'12 '4-'13 '5-'14 '6-'15 '7-'16 '8-'17 COD [mg/l] No '89-'98 '9-'99 '91-' '92-'1 '93-'2 '94-'3 '95-'4 '96-'5 '97-'6 '98-'7 '99-'8 '-'9 '1-'1 '2-'11 '3-'12 '4-'13 '5-'14 '6-'15 '7-'16 '8-'17 COD [mg/l] No.3 Ave.3σ Ave.3σ 25% 75% Ave.
![2 Cl [mg/l] 15 1 5 1 2 3 4 5 COD [mg/l] 6 Cl [mg/l]](/docs-images/98/137890305/images/81-1.jpg "15 1 5 1 2 3 4 5 COD [mg/l] Cl [mg/l] 15 1 5 COD Cl")
![29 217 1 2 3 4 5 COD [mg/l] COD COD 7 No.](/docs-images/98/137890305/images/81-2.jpg "6 COD CODD-COD CODP-COD D-COD P-COD COD 4. No.6 No.")
![6 No.6 COD [mg/l] 3. 2. 1.](/docs-images/98/137890305/images/81-3.jpg ". 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 D-COD P-COD")
81 Ave.3 No.6 No.1 2mg/L COD 6 COD Cl Cl No.2 Cl No.6 No.1 A Cl No.7B 2 No.6No.1 2 No.7 2 No.2 Cl [mg/l] COD [mg/l] 6 Cl [mg/l] COD [mg/l] Cl [mg/l] COD Cl COD [mg/l] COD COD 7 No.6 COD CODD-COD CODP-COD D-COD P-COD COD 4. No.6 No.6 No.6 COD [mg/l] D-COD P-COD D-COD P-COD D-COD P-COD 7 No.6 D-COD P-COD
82 No.6 No.1 COD achl.a 8 Chl.a 9 COD Chl.a Chl.a COD Chl.a 8 Chl.a No.6No.1 2 No.6No Chl.a_calc [μg/l] R² =.7581 Chl.a_calc [μg/l] COD [mg/l] [] 8 COD Chl.a 9 Chl.a No.6 No No.6 No No.6 No Max. [] Ave. Min No.6 No
83 No.6 No.1 COD Cl COD COD COD No.2 12 No.6 No.1 No.6No.14-5 No.6No Cl [mg/l] Cl [mg/l] COD [mg/l] COD [mg/l] 11 No.6 No.1 COD Cl No.24-5 No Cl [mg/l] Cl [mg/l] COD [mg/l] COD [mg/l] 12 No.2 COD Cl No.6 No.1 Chl.a 13 COD Chl.a 14 No.2 No
84 Chl.a COD 2 18 No.6No No Chl.a_calc [μg/l] Chl.a_calc [μg/l] [] [] 13 Chl.a No.6No.1 No Chl.a_calc [μg/l] Chl.a_calc [μg/l] COD [mg/l] COD [mg/l] 14 COD Chl.a No.6 No.1 COD Chl.a 14 2COD
![5 4 COD [mg/l] 3 2 1 1 15 2 25 3 [] 1 5.4.8 14.6 Chl.a [μg/l] 14 Chl.a COD No.6 No.1215 217 T-N Cl 15 T-P Cl 16 No.](/docs-images/98/137890305/images/85-0.jpg "2 T-N T-P No.6 No.1 T-N T-P No.7 No.6 No.1 2 No.6No.1 2 No.7 2 No.2 Cl [mg/l] 15 1 5.2.4.6.8 1 T-N [mg/l] 15 Cl [mg/l] 15 1 5.")
![2.4.6.8 1 T-N [mg/l] Cl [mg/l] 15 1 5 T-N Cl 29 217.2.4.6.8 1 T-N [mg/l] 2 No.6No.1 2 No.7 2 No.2 Cl [mg/l] 15 1 5.](/docs-images/98/137890305/images/85-1.jpg "2.4.6 T-P [mg/l] Cl [mg/l] 15 1 5.2.4.6 T-P [mg/l] 16 T-P Cl 29 217-83 - Cl [mg/l] 15 1 5.2.4.6 T-P [mg/l]")
85 5 4 COD [mg/l] [] Chl.a [μg/l] 14 Chl.a COD No.6 No T-N Cl 15 T-P Cl 16 No.2 T-N T-P No.6 No.1 T-N T-P No.7 No.6 No.1 2 No.6No.1 2 No.7 2 No.2 Cl [mg/l] T-N [mg/l] 15 Cl [mg/l] T-N [mg/l] Cl [mg/l] T-N Cl T-N [mg/l] 2 No.6No.1 2 No.7 2 No.2 Cl [mg/l] T-P [mg/l] Cl [mg/l] T-P [mg/l] 16 T-P Cl Cl [mg/l] T-P [mg/l]
86 217 No.6 No.1 2mg/L COD 75% No.1No.2 No.3 COD 75% COD No.6 No.1 A No.7B No.6 No COD No.6 No.1 Chl.aCOD COD 7 COD P-COD D-COD COD COD COD 1) 29 2) http:// kankyotaisaku/ html ) (217) 4) (217)
87 [ms/m] '17/4/25 '17/5/9 '17/6/19 '17/8/1 '17/9/5 '17/1/ No No No No [mg/l] '17/4/25 '17/5/9 '17/6/19 '17/8/1 '17/9/5 '17/1/ No No No No a [μg/l] '17/4/25 '17/5/9 '17/6/19 '17/8/1 '17/9/5 '17/1/ No < No No No <
88 COD [mg/l] '17/4/25 '17/5/9 '17/6/19 '17/8/1 '17/9/5 '17/1/ No No No No [mg/l] '17/4/25 '17/5/9 '17/6/19 '17/8/1 '17/9/5 '17/1/ No No No No [mg/l] '17/4/25 '17/5/9 '17/6/19 '17/8/1 '17/9/5 '17/1/ No No No No
89 COD [mg/l] '17/4/25 '17/5/9 '17/6/19 '17/8/1 '17/9/5 '17/1/ No No No No [mg/l] '17/4/25 '17/5/9 '17/6/19 '17/8/1 '17/9/5 '17/1/ No No No No [mg/l] '17/4/25 '17/5/9 '17/6/19 '17/8/1 '17/9/5 '17/1/ No No No No
90 mg/kg
91 23mg/kg 4311mg/kg 2.25mg/kg mg/kg
92
93 QuECheERS ppm
94 2 5 1 DNPH
95 TEL 25(231)1231 FAX 25(23)5818 e ise i.r c i t y. n iigata.lg.jp - 4 -
2016/17 P 1 P 8 P13 P18 P23 P28 7 P33 P41 15 P48 P65 GC-MS/MSLC-MS/MS 2016/17 2016 48 1.00 AH3 75.4% HI AH1pdm09 B AH3 D151GD151N HA AH1pdm09 AH3 B 5000 500 2016/17-1 - 2016 2017 78 24 well MDCKLLC-MK2HEp-2HeLa
Microsoft Word - 14_LCMS_アクリルアミド
3--2-3-Iodo-2-propynyl butylcarbamate (IPBC) Carbamic acid, butyl-, 3-iodo-2-propynyl ester Iodocarb CAS 55406-53-6 C 8 H 12 NO 2 I C (g/cm 3 ) (mmhg) log P ow 281.09 (280.9910) 64 68 1.51 1.57 (20 C)
☆H23 13－農薬一斉分析（大垣）.doc
13 56 2011 Examination of Determination of Residual Pesticides in Agricultural Products Yuki OHGAKI, Katsuhiro HAYASHI, Hiroyuki KAWAI, and Kyoko SHIMURA GC/MS 92 LC/MS/MS 60 150 n- 5 GC/MS 92 79 70% LC/MS/MS
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230 C B A D E 50m 558 0 1km (mg L 1 ) T N NO 2 3 N NH 4 N 2.0 0 (a) 2001 1.5 6 20 21 5 1.0 0.5 0.0 2.0 1.5 1.0 0.5 0.0 14:00 17:00 (b) 2001 7 3 4 20:00 23:00 2:00 (h) 5:00 8:00 11:00 10 0 5 10 15
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6.3 6.3.1 6.3.1 6.3.1.1 (1) (2) a) JIS K 0557 A3 b) JIS K 8891 c) (5g/L) d) (10g/L)JIS K 8576 1g 100mL e) (7+993)JIS K 8951 f) JIS K 8322 g) 0.1g 100mL 30mL 1L 500mL6.8mL 50g 1L h) 1L 500mL6.8mL 50g 1L
Microsoft Word - 14_LCMS_アクリルアミド
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平成26年度 化学物質分析法開発報告書
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平成26年度 化学物質分析法開発報告書
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平成25年度 化学物質分析法開発報告書
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March 2008 QIAquick Spin QIAquick PCR Purification Kit PCR 100 bp 10 kb QIAquick Nucleotide Removal Kit 17 40mer DNA 40 bp 10 kb QIAquick Gel Extraction Kit DNA 70 bp 10 kb Sample & Assay Technologies
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1,3- -5,5- -2,4-1,3-Dichloro-5,5-dimethylimidazolidine-2,4-dione 1,3- -5,5-1,3-Dichloro-5,5-dimethylhydantoin CAS 118-52-5 C 5 H 6 Cl 2 N 2 O 2 1,3- -5,5- -2,4-197.1 195.986 1) 132 C 1) 1.5 (d 2 2 )* 1).21
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