VENTANA PD-L1 SP142 Rabbit Monoclonal Antibody OptiView PD-L1 SP142

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1 VENTANA PD-L1 SP142 Rabbit Monoclonal Antibody OptiView PD-L1 SP142

OptiView DAB OptiView Rabbit Monoclonal IHC antibody developed by Spring Bioscience Corporation

2 2 OptiView PD-L1 SP142 OptiView PD-L1 SP142 OptiView PD-L1 SP142 PD-L1 OptiView PD-L1 SP142 PD-L1 OptiView PD-L1 SP142 PD-L1 OptiView PD-L1 SP142 PD-L1 OptiView PD-L1 SP142 TC IC PD-L1 SP142 OptiView DAB OptiView Rabbit Monoclonal IHC antibody developed by Spring Bioscience Corporation Fully Automated With specific and robust signal Highly Reproducible Accurate scoring and educational resources

3 OptiView PD-L1 SP142 3 PD-L1 PD-L1 1 B7-H1 CD kDaCD274 PD-11 T 1 PD-L1 PD-1 B7-1 T 2 PD-L1ADC 3 PD-L1 PD-1 T % 31% 18% NSCLC80 85% 40% 5, NSCLC NSCLC NSCLC B PD-L1 腫瘍浸潤免疫細胞 PD-L1 が PD-1 受容体に結合することにより T 細胞が不活化される PD-1 は PD-L1 と PD-L2 の両方に結合する PD-1 PD-1 PD-L2 PD-L1 PD-L1 腫瘍浸潤免疫細胞上の PD-L1 は活性化 T 細胞の抑制を引き起こすことができる B7-1 (CD80) T 細胞 TCR T 細胞受容体 (TCR) への MHC 抗原複合体の結合は T 細胞シグナル伝達を誘発する PD-1 MHC B7-1 (CD80) PD-L1 PD-L1 は T 細胞表面上の B7-1 および PD-1 受容体と結合する PD-L1 腫瘍細胞腫瘍細胞は免疫介在性の異物排除機構から逃れるために PD-L1 の発現を増加させる腫瘍細胞上の PD-L1 発現は発癌シグナルによって発現が増加される

4 4 OptiView PD-L1 SP142 好酸球顆粒球肥満細胞 B 細胞マクロファージ細胞傷害性 T 細胞制御性 T 細胞樹状細胞骨髄由来免疫抑制細胞 NK 細胞血小板腫瘍細胞ベンタナ OptiView PD-L1(SP142) による染色で免疫細胞の PD-L1 発現の多様性が強調されます大部分はリンパ球マクロファージ樹状細胞および顆粒球です血管内皮細胞周皮細胞線維芽細胞 TME TME T 9,10 TMEPD-L1 PD-1/PD-L1 PD-L1 TPD-1 B T PD-1 B7-1 PD-L1 T PD-L1 PD-L1

5 OptiView PD-L1 SP142 5 OptiView PD-L1 SP142 OptiView PD-L1 SP EZX PD-L1 SP142 RxDx OptiView DAB OptiView 50 ULTRA, XT, GX ULTRA VENTANA PDL1 SP142 XT VENTANA PDL1 SP142 GX VENTANA PDL1 SP142 Baking Antibody Counterstain Post Counterstain VENTANA PD-L1 SP142 Negative Control Hematoxylin 4 min Bluing Reagent 4 min OptiView PD-L1 SP142 OptiView PD-L1 SP142 PD-L1

6 6 OptiView PD-L1 SP142 OptiView PD-L1 SP142 OptiView PD-L1 SP142 1TC 2 IC OptiView PD-L1 SP142TC IC TC IC PD-L1 1 TC20 2 IC20 1 PD-L1 PD-L1TC PD-L1 50% PD-L1 5% 50% PD-L1 1% 5% PD-L1 PD-L1 1% PD-L1IC PD-L1 10% PD-L1 5% 10% PD-L1 1% 5% PD-L1 PD-L1 1% TC3 TC2 TC1 TC0 IC3 IC2 IC1 IC0 TC IC TC IC HE TC IC 3 4 TC IC 3 TC IC 20 4 TC IC 20 IC TC HE TC IC IC TC

7 OptiView PD-L1 SP142 7 References 1. Blank, C and Mackensen, A, Contribution of the PD-L1/PD-1 pathway to T-cell exhaustion: an update on implications for chronic infections and tumor evasion. Cancer Immunol Immunother, : p Butte MJ, Keir ME, Phamduy TB, et al. Programmed death-1 ligand 1 interacts specifically with the B7-1costimulatory molecule to inhibit T cell responses. Immunity. 2007;27 1 : Dong H, Zhu G, Tamada K, Chen L. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nat Med. 1999;5 12 : Herbst RS, Soria JC, Kowanetz M, et al. Predictive correlates of response to the anti-pd-l1 antibody MPDL3280A in cancer patients. Nature. 2014; : , ganjoho.jp, , zangankyo.ncc.go.jp, Siegel R, Naishadham D, Jemal A. Cancer Statistics, CA Cancer J Clin. 2013;63 1 : Howlader N, Noone AM, Krapcho M,et al. eds. SEER Cancer Statistics Review CSR, National Cancer Institute. Published Updated Accessed NCI Dictionary of Cancer Terms. Tumor microenvironment AACR. The Function of Tumor Microenvironment in Cancer Progression Blank, C and Mackensen, A, Contribution of the PD-L1/PD-1 pathway to T-cell exhaustion: an update on implications for chronic infections and tumor evasion. Cancer Immunol Immunother, : p

8 東京都港区港南カスタマーソリューションセンター VENTANA はロシュ社の登録商標です 1085.TD.TMS.3000.PAB01-026A

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