YK250 Human GIP (Active) ELISA FOR RESEARCH LABORATORY USE ONLY FAX: TEL: Website: E-mai

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1 YK250 Human GIP (Active) ELISA FOR RESEARCH LABORATORY USE ONLY FAX: TEL: Website:

3 YK250 Human GIP (Active) ELISA. GIP (glucose-dependent insulinotropic polypeptide)glp-1 (glucagon-like peptide-1)2 GIP 1970 Brown 42 GIP 2) GIP 3,4) Takeda GIP GIP VIP GIP K GIP GIP GIP GIP GIP (1-42) DPP-4 GIP (3-42) DPP GIP GIP GIP (3-42) GIP (1-42) YK250 Human GIP (Active) ELISA GIP (1-42) pg/ml ( pm) 1) 40 duplicate 2) 3.5 3) 4) 50 µl 5) 8 6) 7) 8)

4 . GIP (1-42) GIP (1-42) GIP (1-42) 98% human GIP (1-42) human GIP (3-42) glucagon human GLP-2 GLP-1 (7-36)NH 2 GLP-1 (9-36)NH 2 96 GIP (1-42) HRP GIP (1-42) HRP GIP (1-42) 3

6 µl1 ml nm ml ml 500 pg/ml 0.2 ml 0.2 ml 250 pg/ml 125, 62.5, 31.3, 15.6, 7.8, 3.9 pg/ml 0 pg/ml 3.9 pg/ml250 pg/ml (0.78 pm50.2 pm) 3.9 pg/ml 3.9 pg/ml 2 2 pg/ml ml µl µl (0, 3.9, 7.8, 15.6, 31.3, 62.5, 125, 250 5

7 pg/ml) 50 µl rpm µl rpm µl µl nm/620 nm 13. 5or 4 Parameter GIP GIP GIP 6

8 . 1. EDTA-2Na DPP- inhibitor0.01ml/ml (Catalog No. DPP4 MILLIPORE) BD TM P800 GLP-1, GIP, Glucagon, Ghrelin pg/ml rpm

9 VI Typical standard curve O.D. (450 / 620 nm) Human GIP (1-42) (pg/ml) A Added GIP (1-42) Recovery B Added GIP (1-42) Recovery

10 C Added GIP (1-42) Recovery D Added GIP (1-42) Recovery E Added GIP (1-42) Recovery A B C X X X X % of X X X X % of X X X % of 9

11 D X X X % of E X X X % of GIP (1-42) A Concentration Mean X1 Over X2 Over X X B Concentration Mean X1 Over X2 Over X X C Concentration Mean X1 Over X2 Over X4 Over X

12 D Concentration Mean X1 Over X2 Over X4 Over X E Concentration Mean X1 Over X2 Over X X F Concentration Mean X1 Over X X X G Concentration Mean X1 Over X X X % GIP (1-42) (Human) 100 GIP (3-42) (Human) <0.1 Glucagon <0.1 Human GLP-2 <0.1 GLP-1 (7-36) NH 2 <0.1 GLP-1 (9-36) NH 2 <0.1 11

13 CV CV ~ 250 pg/ml( pm) Brown,J.C., Mutt, V. and Pedersen,R.A. (1970) Further purification of a polypeptide demonstrating enterogastrone activity. J.Physiol. 209, Moody, A. J., Thim, L. & Valverde, I. (1984) The isolation and sequencing of human gastric inhibitory peptide(gip). FEBS Lett. 172, Jörnvall H, Carlquist M, Kwauk S, Otte SC, McIntosh CH, Brown JC, Mutt V. (1981) Amino acid sequence and heterogeneity of gastric inhibitory polypeptide (GIP). FEBS Lett. 123, Moody,A.J., Damm Jorgensen, K.and Thim, L.(1981)Diabetologia 21, 306, abstr. 5. Takeda J, Seino Y, Tanaka K, Fukumoto H, Kayano T, Takahashi H, Mitani T, Kurono M, Suzuki T, Tobe T, et al.(1987) Sequence of an intestinal cdna encoding human gastric inhibitory polypeptide precursor. Proc Natl Acad Sci U S A. 84(20): Pederson, R.A. (1994) in Gut Peptides: Biochemistry and Physiology, eds, Walsh, J.H.& Dockray, G.J. (Raven, New York), pp, Krarup T, Madsbad S, Moody AJ, Regeur L, Faber OK, Holst JJ, Sestoft L.(1983) Diminished immunoreactive gastric inhibitory polypeptide response to a meal in newly diagnosed type I (insulin-dependent) diabetics. J Clin Endocrinol Metab. 56,

14 8. Naitoh R, Miyawaki K, Harada N, Mizunoya W, Toyoda K, Fushiki T, Yamada Y, Seino Y, Inagaki N.(2008) Inhibition of GIP signaling modulates adiponectin levels under high-fat diet in mice. Biochem Biophys Res Commun. 376, Miyawaki K, Yamada Y, Yano H, Niwa H, Ban N, Ihara Y, Kubota A, Fujimoto S, Kajikawa M, Kuroe A, Tsuda K, Hashimoto H, Yamashita T, Jomori T, Tashiro F, Miyazaki J, Seino Y. (1999) Glucose intolerance caused by a defect in the entero-insular axis: a study in gastric inhibitory polypeptide receptor knockout mice. Proc Natl Acad Sci U S A. 96, Miyawaki K, Yamada Y, Ban N, Ihara Y, Tsukiyama K, Zhou H, Fujimoto S, Oku A, Tsuda K, Toyokuni S, Hiai H, Mizunoya W, Fushiki T, Holst JJ, Makino M, Tashita A, Kobara Y, Tsubamoto Y, Jinnouchi T, Jomori T, Seino Y.(2002) Inhibition of gastric inhibitory polypeptide signaling prevents obesity. Nat Med. 8, Tsukiyama K, Yamada Y, Yamada C, Harada N, Kawasaki Y, Ogura M, Bessho K, Li M, Amizuka N, Sato M, Udagawa N, Takahashi N, Tanaka K, Oiso Y, Seino Y. (2006) Gastric inhibitory polypeptide as an endogenous factor promoting new bone formation after food ingestion. Mol Endocrinol. 20, FAX: TEL:

YK254: rat GIP (Total) ELISA Kit

YK254: rat GIP (Total) ELISA Kit 研究用試薬 YK254 Rat GIP (Total) ELISA 取扱説明書 FOR RESEARCH LABORATORY USE ONLY 株式会社矢内原研究所 418-0011 静岡県富士宮市粟倉 2480-1 FAX: 0544-22-2770 TEL: 0544-22-2771 Website: www.yanaihara.co.jp E-mail: ask@yanaihara.co.jp