スライド 1

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1 2 (2007 ) 9:25 10:55 akio@sfc.keio.ac.jp knakahig@sfc.keio.ac.jp ktsuge@ttck.keio.ac.jp TTCK SFC

2 1 2 DNA RNA

3 mrna mrna RNA

4

5

6 ( ) Part 1 DNA Part 2 PCR DNA ( ) Part 3 RNA Part 4

7 Chapter 8 (p ) 9 26 Chapter 9 (p ) 10 3 Chapter 10 mrna (p ) 10 10,17 RNA Chapter 11 Chapter 12

8 Fig.

9

10 RNA HeLa

11 8-1

12 8.1 fluorescence recovery after photobleaching; FRAP

13 8.1.2 DNA HU DNA nm

14 (functional domain) DN I nm

15 RNA Fig.4-37 Fig.4-36

16 Fig.4-39 Fig.4-41

17 8.1.2 (heterochromatin; ) euchromatin;

18 DNA DNA Y 2.8

19 8.4 insulator

20 (positional effect) 8.5

21 8.5

22 8.6

23 (locus control region; LCR) DN I

24

25 Fig nm 30nm

26 8.9 N DNA Fig nm N

27 Fig.4-24 Fig Fig.4-27

28 N P Ac Me u Fig.4-35

29 N N K DNA HAT HDAC Fig.4-35

30 DNA ATP DNA 8-10 DNA DNA DNA DNA DN

31 8.2.2 histone descetylase; HDAC (Sin3,NuRD DNA 5 -CG-3 5 -CNG-3 DNA Fig.7-80

32 DNA DNA DNA Dnmt1 Dnmt3 Dnmt3b 8.11

33 DNA DNA Fig.7-81

34 X genomic imprinting X X chromosome inactivation

35 8.12 DNA CpG MeCP CpG CpG Methyl-CpG-binding protein HDAC DNA MeCP HDAC DNA

36

37 DNA Fig.7-83

38 X (X inactivation) H2A H2A1 DNA Xic Xist 25kb RNA H4 Fig.7-78

39 Fig.7-77 Wikipedia X X X 1 X X

2004

2004 2008 3 20 400 1 1,222 7 1 2 3 55.8 54.8 3 35.8 6 64.0 50.5 93.5 1 1,222 1 1,428 1 1,077 6 64.0 52.5 80.5 56.6 81.5 30.2 1 2 3 7 70.5 1 65.6 2 61.3 3 51.1 1 54.0 2 49.8 3 32.0 68.8 37.0 34.3 2008 3 2 93.5

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