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1 LC ZORBAX Eclipse Plus LC ZORBAX Eclipse

2 .. Agilent ZORBAX ZORBAX Eclipse Plus 2

3 ZORBAX Eclipse Plus LC Eclipse Plus Eclipse Plus Eclipse Plus - - LC ph ECLIPSE Eclipse Plus HPLC HPLC Eclipse Plus 1. ( ) 2. ( ) : 60/ 40 Ace C18 SunFire C18 XBridge C18 Discovery HS C18 Luna C18 (2) Gei C18 XTerra MS C18 3

4 Eclipse Plus Eclipse Plus Eclipse Plus Eclipse Eclipse Plus Agilent ZORBAX Agilent ZORBAX Eclipse Plus XBridge C18, 4.6 x 10 mm, µm N=14000 Tf= ZORBAX, 4.6 x 10 mm, µm N=23000 Tf= ZORBAX Eclipse Plus: N=41000 Tf=1.13 N=60000 Tf=1.00 :10 30% B/1 A:0.1% FA B:0.1% FA (ACN ) :1 ml/ 40 :, Tf = 1.2 Tf = 1.00 Gei C18 Tf = 1.26 Luna C18 (2) Tf = 1.20 SunFire C18 Tf = 1.92 Ace C18 (2) Inertsil ODS (3) Tf = µg 80% : 8mM (ph 7.0) 21 nm 1.0 ml/ 4.6mm x 100 mm x µm 4

5 Eclipse Plus Eclipse Plus ZORBAX Eclipse Plus Eclipse Plus SunFire C18 / (ACN ) USP Tf ACN Tf=1.0 Gei C18 Xterra MS-C18 Luna C18 (2) Tf Diphenhydrae Tf Amitriptyline Ace C18 Discovery C Eclipse Plus N=10747 Tf=1.03 N=10968 Tf= Luna C18 (2) N=9307 Tf=1.09 N=9377 Tf= XBridge C18 N=898 Tf=1.14 N=8737 Tf= :4.6 mm x 10 mm x µm : 60:MeOH 40 :1 ml/ :40 UV 24 nm : :

6 Eclipse Plus µm 3. µm µm Eclipse µm 7 µm Eclipse Plus : 4.6 X 20 mm, µm X 10 mm, 3.µm ? µm Eclipse Plus Rapid Resolution HT (RRHT) Eclipse Plus µm LC O O N + Cl X 0 mm, µm A = (ph 4.) B = (60:40) 1.0mL/: / (6mm x 1.7 µl) :UV 24 nm 4.6 X 0 mm µ X 10 mm µ OCH 3 Tf= 1.00 ZORBAX OCH 3 : :0%ACN: 0% 8mM K2HPO4 (ph7) :1.0mL/: 0.4mg/mL 2.0 µl :UV 24 nm Tf=

7 Eclipse Plus Eclipse Plus 1 1 URL Eclipse Plus Rapid Resolution Rapid Resolution Rapid Resolution Rapid Resolution Rapid Resolution Rapid Resolution HT Rapid Resolution HT Rapid Resolution HT HT HT RR RR RR RR RR RRHT RRHT RRHT (4/pk) (4/pk) ECLIPSE (mm) 9.4 x x x x x x x x x x x x x x x x x x x x x x x x x x x x x 12. (µm) ( Eclipse Plus C18 USP L Eclipse Plus C8 USP L

8 Agilent ZORBAX Eclipse LC Eclipse LC Eclipse LC ph - (Ultra-fast) µm 7 µm 20-60% - ZORBAX Rapid Resolution (RRHT) ( 600 bar) Eclipse LC ZORBAX Eclipse LC... Eclipse XDB ph (ph 2 ph 9) - Agilent ZORBAX 4 Eclipse XDB ZORBAX ZORBAX 8

9 ECLIPSE ZORBAX Rapid Resolution (RRHT) (Ultra-fast) Agilent RRHT LC ZORBAX RRHT LC HPLC 20 : ZORBAX RRHT LC mm 10 mm 1.0 mm 4.6 mm StableBond Eclipse XDB Eclipse Plus 8 ZORBAX µm ( 600 bar) 9% LC LC/MS Agilent 1200 RR LC LC 20 - Web LC 60% ,000 - Web Agilent 1200 Rapid Resolution LC (RRLC) Agilent ZORBAX RRHT µm LC 9

10 ZORBAX RRHT µm LC ( 600 bar 9000 psi) LC Eclipse XDB-C18 Eclipse XDB-C8 SB-C18 SB-C8 SB-CN Extend-C x 10 mm 4.6 x 100 mm 4.6 x 0 mm 4.6 x 30 mm 4.6 x 20 mm 3.0 x 10 mm 3.0 x 100 mm 3.0 x 0 mm 3.0 x 30 mm 3.0 x 20 mm 2.1 x 10 mm 2.1 x 100 mm 2.1 x 0 mm 2.1 x 30 mm 2.1 x 20 mm ZORBAX RRHT µm ( 400 bar 6000 psi) LC Eclipse XDB-C18 Eclipse XDB-C8 SB-C18 SB-C8 Extend-C x 0 mm 2.1 x 0 mm x 0 mm 4.6 x 30 mm 4.6 x 1 mm 2.1 x 0 mm 2.1 x 30 mm 2.1 x 1 mm ZORBAX Eclipse LC : 10

11 ZORBAX Eclipse Rapid Resolution 3.µm HPLC Eclipse XDB-C18 Eclipse XDB-C8 Eclipse XDB-CN Eclipse XDB-Phenyl 4.6 x 10 mm 4.6 x 100 mm 4.6 x 7 mm 4.6 x 0 mm 4.6 x 30 mm 4.6 x 20 mm 3.0 x 10 mm 3.0 x 100 mm 2.1 x 10 mm 2.1 x 100 mm 2.1 x 0 mm 2.1 x 30 mm 2.1 x 20 mm 1.0 x 10 mm 1.0 x 0 mm 1.0 x 30 mm x 10 mm 0. x 3 mm 0.3 x 10 mm x 7 mm 3.0 x 7 mm 4.6 x 12. mm, 4pk 2.1 x 12. mm, 4pk 4.6 & 2.1 mm 1.0 x 17 mm, 3pk ( ) ZORBAX Eclipse µm HPLC Eclipse XDB-C18 Eclipse XDB-C8 Eclipse XDB-CN Eclipse XDB-Phenyl 21.2 x 10 mm 21.2 x 100 mm 21.2 x 0 mm 9.4 x 20 mm 4.6 x 20 mm 4.6 x 10 mm 4.6 x 0 mm 3.0 x 20 mm 3.0 x 10 mm 2.1 x 10 mm 2.1 x 10 mm x 20 mm 0. x 10 mm 0. x 3 mm 0.3 x 20 mm 0.3 x 10 mm 0.3 x 3 mm x 20 mm 4.6 x 10 mm 17 x 7. mm 4.6 x 12. mm, 4pk 4.0 x 4 mm, 10pk 2.1 x 12. mm, 4pk ZORBAX Eclipse 7µm HPLC Eclipse XDB-C18 Eclipse XDB-C8 Eclipse XDB-CN Eclipse XDB-Phenyl 21.2 x 20 mm 21.2 x 10 mm

12 ZORBAX Eclipse LC 40 Agilent ZORBAX Eclipse Plus Eclipse LC Web : : Agilent 1200 Rapid Resolution LC (RRLC) URL : Agilent Agilent Web Agilent Technologies, Inc Printed in Japan Feb 1, JAJP

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