Ł\”ƒNo.3

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1 Fusarium head blightfhb, or scab, caused by several Fusarium species, is a widespread and destructive disease of wheat and barley. FHB resistance is difficult to evaluate because environmental conditions greatly influence FHB infection and development. To reduce environmental influence in such evaluations in barley, we developed a pot-plant method in which the spikes at the anthesis of barley varieties planted in plastic pots were spray-inoculated and hygrothermal conditions after inoculation were controlled to favor the pathogen s infection and development. Degrees of barley resistance in this method correlated highly with those in the cut-spike methodr and field testsr , and year-to-year correlations were highr , suggesting that the pot-plant method is useful in accurate, stable FHB resistance evaluation in barley. : Fusarium head blight, scab, barley, resistance, testing method, pot-plant method.

2 Fusarium Fusarium graminearum Schwabeteleomorph Gibberella zeaeschw. Petch 1993Parry et al Miedaner Gocho and Hirai Miedaner 1997Rudd et al Kolb et al Parry et al F. graminearum Parry et al Gocho and Hirai r0.6 Rudd et al

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17 r r r Ban, T. and K. Suenaga2000Genetic analysis of resistance to Fusarium head blight caused by Fusarium graminearum in Chinese wheat cultivar Sumai 3 and the Japanese cultivar Saikai 165. Euphytica, 113, , Gocho, H. and T. Hirai1987Varietal resistance to scab in barley. Barley Genetics, , , Kolb, F.L., G-H. Bai, G.J. Muehlbauer, J.A. Anderson, K.P. Smith and G. Fedak2001 Symposium on genetic solutions to Fusarium head blight in wheat and barley: challenges, opportunities, and imperatives. Crop Sci. 41, Miedaner, T.1997Breeding wheat and rye for resistance to Fusarium diseases. Plant Breeding

18 116, , , Parry, D. W., P. Jenkinson and L. McLeod1995 Fusarium ear blightscabin small grain cereals-a review. Plant Pathology 44, Rudd, J.C., R.D. Horsley, A.L. McKendry and E.M. Elias2001Host plant resistance genes for Fusarium head blight: Sources, mechanisms, and utility in conventional breeding systems. Crop Sci. 41, , , , , 226.

19 The pot-plant method provides accurate, stable testing of the resistance to Fusarium head blight in barley. Megumi YOSHIDA, Naoyuki KAWADA and Takuji TOHNOOKA Fusarium head blightfhb, or scab, caused by several Fusarium species, is a widespread and destructive disease of wheat and barley. FHB resistance is difficult to evaluate because environmental conditions greatly influence FHB infection and development. To reduce the environmental influence in such evaluations in barley, we developed a pot-plant method in which the spikes at the anthesis of barley varieties planted in plastic pots were spray-inoculated and hygrothermal conditions after inoculation were controlled to favor the pathogen s infection and development. Degrees of resistance of barley varieties in this method correlated highly with those in the cut-spike method r and field testsr , and year-to-year correlations were high r , suggesting that the pot-plant method is useful in accurate, stable evaluation of FHB resistance in barley. We study and compare characters of the pot-plant method and other testing methods. Received 15 January, 2003 National Agricultural Research Center for Kyushu Okinawa Region

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[2] 1. 2. 2 2. 1, [3] 2. 2 [4] 2. 3 BABOK BABOK(Business Analysis Body of Knowledge) BABOK IIBA(International Institute of Business Analysis) BABOK 7 32 (2015 ) [2] Projects of the short term increase at present. In order to let projects complete without rework and delays, it is important that request for proposals (RFP) are written by reflecting precisely