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1 DNA typing SSO & SSP Japanese Red cross fukuoka hospital HLA Hiroki Hashiguchi 2010/09/19 Tokyo
2 -Volumea. 10μg/100μL b. 20μg/200μL (SSP) HLA-A HLA-B HLA-C HLA-DRB1 HLA-DQB1 ID allele #1 allele #2 allele #1 allele #2 allele #1 allele #2 allele #1 allele #2 allele #1 allele #2 H2201 A*0206 A*0207 B*4601 B*5901 Cw* DRB1*040 5 DRB1*080 3 DQB1*040 1 DQB1*060 1 H2202 A*2402 A*2402 B*1501 B*1518 Cw*0102 Cw*0704 DRB1*080 2 DRB1*140 5 DQB1*030 2 DQB1*050 3 H2203 A*1101 A*3101 B*4002 B*4006 Cw*0304 Cw*0801 DRB1*040 5 DRB1*041 0 DQB1*040 1 DQB1*040 2 H2204 A*1101 A*2402 B*1501 B*5101 Cw*0401 Cw*1402 DRB1*040 6 DRB1*140 3 DQB1*030 1 DQB1*030 2 H2201 A2 A2 B46 B59 Cw1 - DR4 DR8 DQ4 DQ6 H2202 A24 - B62 B71 Cw1 Cw7 DR8 DR14 DQ8 DQ5 H2203 A11 A31 B61 B61 Cw10 Cw8 DR4 DR4 DQ4 DQ4 H2204 A11 A24 B62 B51 Cw4 Cw14 DR4 DR1403 /DR14 DQ7 DQ8
3 Kit Sample.a Sample.b SSP (JPN) SSO (RELI) DNA 100ng/μL 100ng/μL 100ng/μL (25-200) 13-15ng/μL Vol. 100μL 200μL 111μL/test 15μL/locus
4 SSP SSO Other % 33% 27%
5 SSP(23) SSO(19) 輸血関連 (8) 輸血関連 (5) 臓器移植 (15) 臓器移植 (14) 造血幹 (5) 造血幹 (3) その他 (3) ( 重複を含む )
6 記載不備の一例誤 ) 1 *26:01/02/10/+ ローカス名の記入漏れ 2 A 26:01/02/10/+ * マークの記入漏れ 3 A*26:01/26:02/26:10/+ 4 桁 /2 桁表記 4 B*15:01/26N/28/+ Null alleleの表記 正 ) A*26:01/02/10/+ B*15:01/26/27/+
7 Invitrogen (Dynal) RELI RELI - Assay time- -sample vol.- PCR 120min 15μL/locus Hybri 90min Total 210mim
8 A B B C DR DQ Probe Lot Lab
9 A B Cw DRB 1 DRB 345 DQB
10 Lab.ID HLA-A HLA-B HLA-C 22D03 A*02:06/10/21/+ A*02:07/15/18 B*46:01 B*59:01 22D05 A*02:06/07/10/+ A*02:07/15/18/+ B*46:01/02/09/+ B*59:01 C*01:02/06/07/+ - 22D10 A*02:06/10/21/+ A*02:07/15/18 B*46:01/02/09/+ B*59:01 22D11 A*02:06/10/21/+ A:*02:07/15/18 B*46:01/02/09/+ B*59:01 C*01:02/06/07/+ - 22D12 A*02:06/10/21/+ A*02:07/15/18 B*46:01/02/09/+ B*59:01/05 C*01:02/06/07/+ - 22D18 A*02:06 A*02:07/15N/18 B*46:01 B*59:01 22D19 A*02:06/10/21/+ A*02:07/15/18/+ B*46:01/02/09/+ B*5901 C*01:02/06/07/+ - 22D22 A*02:06/07/10/+ - B*46:01/02/09/+ B*59:01 22D26 A*02:06/10/21/+ A*02:07/15N/18/+ B*46:01/02/09/+ B*59:01 22D28 A*02:06/10 A*02:07/18 B*46:01/02 B*59:01 22D29 A*02:06/07/10/+ ー B*46:01/02/09/+ B*59:01 22D31 A*02:06/07/10/+ A*02:07/15/18/+ B*46:01/02/09/+ B*59:01 22D33 A* 02:06/10/21/+ A*02:07/18 B*46:01/02 B*59:01 Cw*01:02/06/07/+ Cw*01:16 22D35 A*02:06/10/21/+ A*02:07/15/18 B*46:01/02/09/+ B*59:01 22D38 A*02:07/15N/18 A*02:06/10/21/+ B*46:01/02/09/+ B*59:01 22D41 A*02:06/10/21/+ A*02:07/15/18 B*46:01/02/09/+ B*59:01 22D44 A*02:06/10/21/+ A*02:07/15/18 B*46:01/02 B*59:01 C*01:02/06/07/+ - 22D50 A*02 A*02 B*46:01/02/15/+ B*59:01 22D52 A*02:06/10/12/+ A*02:07/15N/18 B*46:01/02/04/+ B*59:01
11 Lab.ID HLA-A HLA-B HLA-C 22D03 A*24:02/09/11/+ - B*15:01/26/33/+ B*15:18/72 22D05 A*024:02/05/09/+ - B*15:01/18/26/+ B*15:18/35/66/+ C*01:02/06/07/+ C*07:04/11/12/+ 22D10 A*24:02/05/09/+ - SSPの追加実施 SSPの追加実施 22D11 A*24:02/05/09/+ - B*15:01/26/27/+ B*15:18 C*01:02/06/07/+ C*07:04/11/12/+ 22D12 A*24:02/09/11/+ - B*15:01/26/27/+ B*15:18/72/134 C*01:02/06/07/+ C*07:04/11/12/+ 22D18 A*24:02 - B*15:01 B*15:18 22D19 A*24:02/09N/11N/+ - B*15:01/26N/27/+ B*15:18/134/133/+ C*01:02/06/07/+ C*07:04/11/12/+ 22D22 A*24:02/05/09/+ - B*15:01/18/26/+ - 22D26 A*24:02/17/20/+ - B*15:01/26N/27/+ B*15:18/72 22D28 A*24:02/05/20 - B*15:01 B*15:18 22D29 A*24:02/05/09/+ ー B*15:01/18/26/+ - 22D31 A*24:02/05/09/+ - B*15:01/18/26/+ B*15:18/35/66/+ 22D33 A* 24:02/05/17/+ A*24:88 B*15:01/27/57/+ B*15:18/134 Cw*01:02/06/07/+ Cw*07:04/11/12/+ 22D35 A*24:02/09/11/+ - B*15:01/26/27/+ B*15:18/72/134 22D38 A*24:02/05/09N/+ - B*15:01/26N/27/+ B*15:18/72/134 22D41 A*24:02/05/09/+ - B*15:01/26/27/+ B*15:18/72/134 22D44 A*24:02/09/11/+ - B*15:01/26/33/+ B*15:18/72 C*01:02/06/07/+ C*07:04/11/12/+ 22D50 A*24:02/09/11/+ - B*15:01/26/27/+ B*15:18 22D52 A*24:02/05/09N/+ - B*15 B*15
12 Lab.ID HLA-A HLA-B HLA-C 22D03 A*11:01/02/03/+ A*31:01/02/06/+ B*40:02/35/37 B*40:06 22D05 A*11:01/02/03/+ A*31:01/02/06/+ B*40:02/06/27/+ B*40:06/70/82/+ C*03:04/05/08/+ C*08:01/03/04/+ 22D10 A*11:01/02/03/+ A*31:01/02/06/+ B*40:02/27/29/+ B*40:06/70/83 22D11 A*11:01/02/03/+ A*31:01/02/06/+ B*40:02/27/29 B*40:06/70 C*03:04/05/08/+ C*08:01/03/04/+ 22D12 A*11:01/02/03/+ A*31:01/02/06/+ B*40:02/27/29/+ B*40:06/70/83/+ C*03:04/08/09/+ C*08:01/03/04/+ 22D18 A*11:01 A*31:01/02/06 B*40:02/35/37 B*40:06 22D19 A*11:01/02/03/+ A*31:01/02/06/+ B*40:02/29/37/+ B*40:06 C*03:04/08/09/+ C*08:01/03/04/+ 22D22 A*11:01/02/03/+ A*31:01/02/06/+ B*40:02/27/29/+ B*40:06/70/83 22D26 A*11:01/02/03/+ A*31:01/02/06/+ B*40:02/27/29/+ B*40:06/70/83 22D28 A*11:01/02/03 A*31:01/06 B*40:02/29 B*40:06 22D29 A*11:01/02/03/+ A*31:01/02/06/+ B*40:02/06/27/+ - 22D31 A*11:01/02/03/+ A*31:01/02/06/+ B*40:02/06/27/+ B*40:06/70/82/+ 22D33 A*11:01/02/03/+ A*31:01/02/06/+ B*40:02/29/37/+ B*40:06/70 Cw*03:04/05/08/+ Cw*08:01/03/04/+ 22D35 A*11:01/02/03/+ A*31:01/02/06/+ B*40:02/27/29/+ B*40:06/70 22D38 A*11:01/02/03/+ A*31:01/02/06/+ B*40:06/70/83 B*40:02/27/29/+ 22D41 A*11:01/02/03/+ A*31:02/02/06/+ B*40:02/27/29/+ B*40:06/70/83 22D44 A*11:01/02/03/+ A*31:01/02/06/+ B*40:02/35/37 B*40:06 C*03:10/29 C*08:01/03/04/+ 22D50 A*11:01/02/03/+ A*31:01/02/06/+ B*40:06 B*40:19 22D52 A*11:01/02/03/+ A*31:01/02/06/+ B*40:02/27/29/+ B*40:06/70/83
13 Lab.ID HLA-A HLA-B HLA-C 22D03 A*11:01/02/03/+ A*24:02/09/11/+ B*51:01/03/09/+ B*15:01/26/33/+ 22D05 A*11:01/02/03/+ A*24:02/05/07/+ B*51:01/02/03/+ B*15:01/04/05/+ C*04:01/05/06/+ C*14:02/04/07/+ 22D10 A*11:01/02/03/+ A*24:02/05/09/+ SSPの追加実施 SSPの追加実施 22D11 A*11:01/02/03/+ A*24:02/05/07/+ B*15:01/04/08/+ B*51:01/03/11/+ C*04:01/05/08/+ C*14:02/07/11/+ 22D12 A*11:01/02/03/+ A*24:02/09/11/+ B*15:01/26/27/+ B*51:01/03/09/+ C*04:01/05/09/+ C*14:02/03/08 22D18 A*11:01 A*24:02 B*15:01 B*51:01 22D19 A*11:01/02/03/+ A*24:02/05/07/+ B*15:01//+ B*51:01//+ C*04:01/05/09N/+ C*14:02/04/07N/+ 22D22 A*11:01/02/03/+ A*24:02/05/07/+ B*15:01/04/05/+ B*51:01/02/03/+ 22D26 A*11:01/02/03/+ A*24:02/05/15/+ B*15:01//+ B*51:01//+ 22D28 A*11:01/02/03 A*24:02 B*15:01/27/33 B*51:01/03 22D29 A*11:01/02/03/+ A*24:02/05/07/+ B*15:01//+ B*35//+ 22D31 A*11:01/02/03/+ A*24:02/19/44/+ B*51:01/02/03/+ B*15:01/04/05/+ 22D33 A*11:01/02/03/+ A*24:02/05/10/+ B*15:01/08 B*51/52 Cw*04:01/05/08/+ Cw*14:02/04 22D35 A*11:01/02/03/+ A*24:02/09/11/+ B*15:01/26/27/+ B*51:01/03/09/+ 22D38 A*11:01/02/03/+ A*24:02/05/07/+ B*15:01//+ B*51:01//+ 22D41 A*11:01/02/03/+ A*24:02/05/07/+ B*15:01//+ B*51:01//+ 22D44 A*11:01/02/03/+ A*24:02/09/11/+ B*51:01/03/09/+ B*15:01/26/33/+ C*04:01/05/09/+ C*14:02/07 22D50 A*11:23/24 A*24:02/09/11/+ B*35:43/67/79 B*52:01/09 22D52 A*11:01/02/03/+ A*24:02/05/07/+ B*15:01/04/05//+ B*51:01/02/03//+
14 Lab.ID HLA-DRB1 HLA-DRB3/4/5 HLA-DQB1 22D03-2 DRB1*04:05/10/24/+ DRB1*08:03/14/23/+ DRB4*01 22D05-2 DRB1*04:05/10/24/+ DRB1*08:03/10/14/+ DRB4*01/02 - DQB1*04:01 DQB1*06:01 22D10-2 DRB1*04:05/10/24/+ DRB1*08:03/10/14/+ DRB4*01/02-22D11-2 DRB1*04:05/10/24/+ DRB1*08:03/10/14/+ DRB4*01:01/02/03/+ - 22D12-2 DRB1*04:05/10/24/+ DRB1*08:03/14/23/+ DRB4*01:01/02/03/+ - DQB1*04:01 DQB1*06:01 22D18-2 DRB1*04:05 DRB1*08:03 22D19-2 DRB1*04:05/24/28/+ DRB1*08:03/14/23/+ DRB4*01:01/04/03/05/+ 22D29-2 DRB1*04:04/05/08/+ DRB1*08:03/10/14/+ DRB4*01/02 22D22-2 DRB1*04:05/10/24/+ DRB1*08:03/10/14/+ DRB4*01/02-22D26-2 DR*04:05/24/28/+ DR*08:03/14/23/+ 22D28-2 DRB1*04:05/10 DRB1*08:03/10 DRB4*01:01/03/05/+ DRB4*01:02/04 22D31-2 DRB1*04:05/10/24/+ DRB1*08:03/10/14/+ DRB4*01:01/02/03/+ 22D33-2 DRB1*04:05/10/24/+ DRB1*08:03/14/23/+ 22D35-2 DRB1*04:05/10/24/+ DRB1*08:03/14/23/+ DRB4*01:01/03-22D38-2 DRB1*04:05/10/24/+ DRB1*08:03/10/14/+ DRB4*01 DRB4*02 22D41-2 DRB1*04:05/10/24/+ DRB1*08:03/10/14/+ DRB4*01:01/02/03/+ - 22D44-2 DRB1*04:05/10/24/+ DRB1*08:03/14/23/+ DRB4*01:01/03/05/+ - DQB1*04:01 DQB1*06:01 22D50-2 DRB1*04:05/24/28/+ DRB1*08:03/14/23/+ DRB4*01:01/03/05/+ 22D52-2 DRB1*04:05/10/24/+ DRB1*08:03/10/14/+ DRB4 -
15 Lab.ID HLA-DRB1 HLA-DRB3/4/5 HLA-DQB1 22D03-2 DRB1*08:02/09/12 DRB1*14:05/08/45 DRB3*02:02 22D05-2 DRB1*08:02/04/07/+ DRB1*1405/23/43/+ DRB3*02:01/02/03/+ - DQB1*03:02/07 DQB1*05:03 22D10-2 SSO の追加実施 (DRB1*03/11/13/14) 22D10-3 DRB1*14:05/23/45/+ - SSO の追加実施 (DRB1*03/11/13/14) DRB3*02:01/02/03/+ - 22D11-2 DRB1*08:02/04/07/+ DRB1*014:05/14/23/+ DRB3*02:01/02/03/+ - 22D12-2 DRB1*08:02 DRB1*14:05/23/45/+ DRB3*02:01/02/03/+ - DQB1*03:02 DQB1*05:03 22D18-2 DRB1*08:09/21 DRB1*14:05/23 22D19-2 DRB1*14:05//+ DRB1*08:02//+ DRB3* D29-2 DRB1*14:05/15/23/+ ー DRB3*02:01/02/03/+ 22D22-2 DRB1*08:02/04/07/+ DRB1*14:05/14/23/+ DRB3*02:01/02/03/+ - 22D26-2 DR*08:02 DR*14:05/08/23/+ 22D28-2 DRB1*08:02/04 DRB1*14:05/23 DRB3*02:01/02/03/+ DRB3*02:02/10/11/+ 22D31-2 DRB1*08:02/04/07/+ DRB1*14:05/14/15/+ DRB3*02:01/02/03/+ 22D33-2 DRB1*08:02//+ DRB1*14:05//+ 22D35-2 DRB1*08:02/04/28 DRB1*11:52/14:05/+ DRB3*02:02-22D38-2 DRB1*08:02/04/07/+ DRB1*14:05/23/43/+ DRB3*02-22D41-2 DRB1*08:02/04/07/+ DRB1*14:05/23/43/+ DRB3*02:01/02/03/+ - 22D44-2 DRB1*08:02/09/12 DRB1*14:05/08/45 DRB3*02:01/02/03/+ - DQB1*03:02 DQB1*05:03 22D50-2 DRB1*08:02 DRB1*14:05/23 DRB3*02:02/10/11/+ 22D52-2 DRB1*08:02/04/07//+ DRB1*14:05/08/14//+ DRB3 -
16 Lab.ID HLA-DRB1 HLA-DRB3/4/5 HLA-DQB1 22D03-2 DRB1*04:05/10/24/+ - DRB4*01 22D05-2 DRB1*04:03/05/10/+ DRB*04:05/10/11/+ DRB4*01/02 - DQB1*04:01 DQB1*04:02 22D10-2 DRB1*04 DRB1*04 DRB4*01/02 DRB4*01/03 22D11-2 DRB1*04:03/04/05/+ - DRB4*01:02/03/04/+ - 22D12-2 DRB1*04:03/05/10/+ - DRB4*01:01/02/03/+ - DQB1*04:01 DQB1*04:02 22D18-2 DRB1*04:05-22D19-2 DRB1*04:03 DRB1*04:05/24/28/+ DRB4*01:01/03/05/+ 22D29-2 DRB1*04:05/08/11/+ ー DRB4*01/02 22D22-2 DRB1*04:03/05/10/+ - DRB4*01/02-22D26-2 DR*04:10 DR*04:05/24/28/+ 22D28-2 DRB1*04:05/10/17 - DRB4*01:01/03/05/+ DRB4*01:02/04 22D31-2 DRB1*04:03/05/10/+ - DRB4*01:01/02/03/+ 22D33-2 DRB1*04 DRB1*04 22D35-2 DRB1*04:05/10/24/+ - DRB4*01:01/03-22D38-2 DRB1*04:05/24/28/+ - DRB4*01/02-22D41-2 DRB1*04:03/05/10/+ - DRB4*01:01/02/03/+ - 22D44-2 DRB1*04:05/24/28+ DRB*04:10 DRB4*01:01/03/05/+ - DQB1*04:01 DQB1*04:02 22D50-2 DRB1*04:05/24/28/+ - DRB4*01:01/03/05/+ 22D52-2 DRB1*04:03/05/10/+ - DRB4 -
17 Lab.ID HLA-DRB1 HLA-DRB3/4/5 HLA-DQB1 22D03-2 DRB1*04:03/06/07/+ DRB1*14:03/12 DRB3*01:01 DRB4*01 22D05-2 DRB1*04:03/06/07/+ DRB1*13:62/14:03/12/+ DRB3*01:01/04/11/+ DRB4*01/02 DQB1*03:01/02/19/+ DQB1*03:02 22D10-2 DRB1*04 DRB1*13/14 DRB3*01:01/04/11/+ DRB4*01/02 22D10-3 DRB1*14 DRB1*13/14 22D11-2 DRB1*04:03/06/07/+ DRB1*14:03/12/18/+ DRB3*01:01/04/11 DRB4*01:01/02/03/+ 22D12-2 DRB1*04:03/06/07/+ DRB1*14:03 DRB3*01:01/04/11/ DRB4*01:01/02/03/+ DQB1*03:01/19/21/+ DQB1*03:02 22D18-2 DRB1*04:03/06 DRB1*14:03 22D19-2 DRB1*04:03/06/07/+ DRB1*14:03//+ DRB3*01:01/04/11/+ DRB4*01:03//+ 22D29-2 DRB1*03:02/27 DRB1*04:58 DRB3*01:01/04/11 DRB4*01/02 22D22-2 DRB1*04:03/06/07/+ DRB1*14:03/12/18/+ DRB3*01:01/04/11/+ DRB4*01/02 22D26-2 DR*04:03/06/39/+ DR*14:03 22D28-2 DRB1*04:03/07 DRB1*14:03/12 22D31-2 DRB1*04:03/06/07/+ DRB1*14:03/12/18/+ DRB3*01:01/04/11 DRB4*01:01/02/03/+ 22D33-2 DRB1*04:03/06/07/+ DRB1*13/14 22D35-2 DRB1*04:03/06/07/+ DRB1*14:03/12 DRB3*01:01 DRB4*01:01/03 22D38-2 DRB1*04:03/06/07/+ DRB1*14:03/27 DRB3*01 DRB4*01/02 22D41-2 DRB1*04:03/06/07/+ DRB1*14:03/12/27 DRB4*01:01/02/03/+ DRB3*01:01/04/11/+ 22D44-2 DRB1*04:03/06/07/+ DRB1*14:03/12 DRB4*02:01/03/05/+ DRB3*02:01/02/03/+ DQB1*03:01/19 DQB1*03:02 22D50-2 DRB1*04:07/20/47/+ DRB1*14:03 DRB3*01:01/04/11 DRB4*01:01/03/05/+ 22D52-2 DRB1*04:03/06/07/+ DRB1*14:03/12/18//+ DRB3 DRB4
18 結果 Sample Lab.No アリル表記 HLA 型 アリル表記 HLA 型 # D33 C*01:16 Cw*1 C*01:02 Cw1 # D33 A*24:88 A24 A*24:02 A24 # D19 C*01:01/06/07/+ Cw1 Cw*0102 Cw1 # D18 DRB1*08:09/21 DR8 DRB1*0802 DR8 # D29 - DRB1*0802 DR8 # D50 B*40:19 B61 B*4002 B61 # D44 C*03:10/29 Cw3 Cw*0304 Cw10 # D DRB1*04:10 DR4 # D19 DRB1*04:03 DR4 DRB1*04:10 DR4 # D29 DRB1*04:05/08/11/+ DRB1*04:05, 04:10 DR4 # D50 A*11:23/24 A11 A*1101 A11 # D29 B*35//+ B*5101 B51 # D50 B*35:43/67/79 B35 B*5101 B51 # D50 B*52:01/09 B52 B*1501 B62 # D50 DRB1*04:07/20/47/+ DR4 DRB1*0406 DR4 # D29 DRB1*03:02/27 DRB1*1403 DR14 正解 # D29 DRB1*04:58 DRB1*0406 DR4
19 One Lambda Micro SSP invitrogen UniTray Micro SSP - Assay time- -sample vol.- PCR 85min 111μL SSP-JPN Gel 3-5min Total 90mim
20
21 Lab.ID HLA-A HLA-B HLA-C 22D01 A*02:01/02/03/+ - B*46:01/02/05/+ B*59:01 A*02:06/106/126/+ A*02:07 22D05 B*46:01/03/05/+ C*01:02/25/26/+ - 22D06 B*46:01/02/05/+ B*59:01/02/03/+ 22D06-2 A*02:01/02/03/+ - 22D06-3 C*01:02/03/05/+ - 22D08 A*02:01/02/03/+ - B*59:01/02/03/+ B*46:01/02/05+ C*01:02/03/05/+ - 22D12 A*02:01/02/03/+ - B*46:01/02/05/+ B*59:01/02/03 C*01:02/03/05/+ - 22D14 A*02:01/02/03/+ - B*46:01/02/05/+ B*59:01/03/05 22D16 A*02:01/03/06/+ - B*46:01/02 B*59:01 C*01:02/03-22D17 A*02:01/06/07/+ - B*46:01/02/05/+ B*59:01/02/03/+ C*01:02/03/05/+ - 22D20 A*02:01/02/03/+ - B*46:01/02/05/+ B*59:01/02/03/+ C*01:02/03/05/+ - A*02:06 A*02:07 22D21 B*46:01 C*01:02 実施なし 22D23 A*02:01/02/03/+ - B*46:01/02/05/+ B*59:01/02/03 C*01:02/03/05/+ - 22D30 A*02:01/02/03/+ - B*46:01/02/05/+ B*59:01/02/03/ C*01:02/03/05/+ - 22D39 A*02:07 A*02:01/105/111/+ 22D47 A*02:01/02/03/+ - B*46:01/02/05/+ B*59:01/02/03 C*01:02/03/05/+ - 22D48 A2 A2 B46 B59 Cw1 Cw1 22D51 A2 - B46 B59 Cw1-22D52 A*02:01/02/03/+ - B*46:01/02/05/+ B*59:01/02/03/+
22 Lab.ID HLA-A HLA-B HLA-C 22D01 A*24:02/03/04/+ - B*15:01/07/26/+ B*15:10/18/37/+ A*24:02/69/70/+ - 22D05 B*15:01/26/71/+ B*15:18/72/134/+ C*01:02/25/26/+ C*07:04/63 22D06 B*15:01/04/07/+ B*15:10/18/29/+ C*01:02/03/05/+ C*07:04/11/12/+ 22D06-2 A*24:02/04/05/+ - 22D08 A*24:02/03/04/+ - B*15:01/04/07/+ B*15:10/18/37/+ C*01:02/03/05/+ C*07:04/11/12/+ 22D10 B*15:01/66/71/+ B*15:18/72/133/+ 22D12 A*24:02/03/04/+ - B*15:01/04/07/+ B*15:10/18/37/+ C*01:02/03/05/+ C*07:04/11/12/+ 22D14 A*24:02/03/04/+ - B*15:01/07/26/+ B*15:10/18/37/+ 22D16 A*24:02/04/07/+ - B*15:01/07/27/+ B*15:18 C*01:02/03 C*07:04 22D17 A*24:02/03/04/+ - B*15:01/04/07/+ B*15:10/18/37/+ C*01:02/03/05/+ C*07:04/11/12/+ 22D20 A*24:01/02/03/+ - B*15:01/04/07/+ B*15:10/18/37/+ C*01:02/03/05/+ C*07:04/11/12/+ A*24:02 実施なし 22D21 B*15:01 B*15:18 C*01:02 C*07:04 22D23 A*24:02/03/04/+ - B*15:01/04/07/+ B*15:10/18/37/+ C*01:02/03/05/+ C*07:04/11/12/+ 22D30 A*24:02/03/04/+ - B*15:01/04/07/+ B*15:10/18/37/+ C*01:02/03/05/+ C*07:04/11/12/+ 22D39 A*24:02/69/70/+ - B*15:01/71/75/+ B*1518/134/153 22D47 A*24:02/03/04/+ - B*15:01/04/07/+ B*15:10/18/37/+ C*01:02/03/05/+ C*07:04/11/12/+ 22D48 A24 A24 B62 B71 Cw1 Cw7 22D51 A24 - B62 B71 Cw1 Cw7 22D52 A*24:02/03/04/+ - B*15:01/04/07/+ B*15:10/18/37/+
23 Lab.ID HLA-A HLA-B HLA-C 22D01 A*11:01/02/03/+ A*31:01/02/03/+ B*40:06/44/70/+ B*40:02/03/08/+ A*11:01/15/19/+ A*31:01/11/12/+ 22D05 B*40:02/56/57/+ B*40:06/86 C*03:04/57 C*08:01/20/22/+ 22D06 A*11:01/02/03/+ A*31:01/02/03/+ B*40:02/03/08/+ B*40:06/44/70/+ C*08:01/02/03/+ C*03:02/04/05/+ 22D08 A*11:01//+ A*31:01//+ B*40:02/03/08/+ B*40:06/44/70 C*03:02/04/05/+ C*08:01/02/03/+ 22D12 A*11:01/02/03/+ A*31:01/02/03/+ B*40:02/03/08/+ B*40:06/44/70 C*03:02/04/05/+ C*08:01/02/03/+ 22D14 A*11:01/02/03/+ A*31:01/02/03/+ B*40:02/03/08/+ B*40:06/44/70 22D16 A*11:01/02 A*31:01/05 B*40:02/03/29/+ B*40:06 C*03:02/04 C*08:01/03 22D17 A*11:01/02/03/+ A*31:01/02/03/+ B*40:02/03/08/+ B*40:06/44/70 C*03:02/04/05/+ C*08:01/02/03/+ 22D20 A*11:01/02/03/+ A*31:01/02/03/+ B*40:02/03/08/+ B*40:06/44/70 C*03:02/04/05/+ C*08:01/02/03/+ A*11:01 A*31:01 22D21 B*40:02 B*40:06 C*03:04 C*08:01 22D23 A*11:01/02/03/+ A*31:01/02/03/+ B*40:02/03/08/+ B*40:06/44/70 C*08:01/02/03/+ C*03:02/04/05/+ 22D30 A*11:01/02/03/+ A*31:01/02/03/+ B*40:02/03/08/+ B*04:06/44/70 C*03:02/04/05/+ C*08:01/02/03/+ 22D39 B*40:02/56/57/+ B*40:06/86 22D47 A*11:01/02/03/+ A*31:01/02/03/+ B*40:02/03/08/+ B*40:06/44/70 C*03:02/04/05/+ C*08:01/02/03/+ 22D48 A11 A31 B61 B61 Cw8 Cw10 22D51 A11 A31 B61 B61 Cw8 Cw10 22D52 A*11:01/02/03//+ A*31:01/02/03//+ B*40 B*40/27
24 Lab.ID HLA-A HLA-B HLA-C 22D01 A*11:01/02/03/+ A*24:02/03/04/+ B*15:01/07/26/+ B*51:01/03/07/+ A*11:01/15/19/+ A*24:02/69/70/+ 22D05 B*15:01/71/75/+ B*51:01/37/38/+ C*04:01/04/12/+ C*14:02/08/09 22D06 A*11:01/02/03/+ A*24:02/03/04/+ B*51:01/02/03/+ B*15:01/04/06/+ 22D06-3 C*04:01/02/03/+ C*14:02/03/04/+ 22D08 A*11:01//+ A*24:02/03/04/+ B*51:01/02/03/+ B*15:01/04/06/+ C*04:01//+ C*14:02//+ 22D10 B*15:01/05/06/+ B*51:01/37/38/+ 22D12 A*24:02/03/04/+ - B*15:01/04/07/+ B*51:01/03/07/+ C*04:01/02/03/+ C*14:02/05/06/+ 22D14 A*24:02/03/04/+ A*11:01/02/03/+ B*15:01/07/26/+ B*51:01/03/07/+ 22D16 A*11:01/02 A*24:02/04/07/+ B*15:01/07/27/+ B*51:01/03 C*04:01 C*14:02 22D17 A*11:01/02/03/+ A*24:02/03/04/+ B*15:01/04/07/+ B*51:01/03/07/+ C*04:01/02/03/+ C*14:02/05/06/+ 22D20 A*11:01/02/03/+ A*24:02/03/04/+ B*51:01/03/07/+ B*15:01/04/07/+ C*04:01/02/03/+ C*14:02/05/06/+ A*11:01 A*24:02 22D21 B*15:01 B*51:01 C*04:01 C*14:02 22D23 A*11:01/02/03/+ A*24:02/03/04/+ B*15:01/04/07/+ B*51:01/03/07/+ C*04:01/03/04/+ C*14:02/05/06/+ 22D30 A*11:01/02/03/+ A*24:02/03/04/+ B*51:01/03/07/+ B*15:01/04/07/+ C*04:01/02/03/+ C*14:02/05/06/+ 22D33 B*15 B*51 22D39 A*24:02/69/70/+ 22D47 A*11:01/02/03/+ A*24:02/03/04/+ B*15:01/04/07/+ B*51:01/03/07/+ C*04:01/02/03/+ C*14:02/05/06/+ 22D48 A11 A24 B51 B62 Cw4 Cw4 22D51 A11 A24 B51 B62 Cw4 Cw14 22D52 A*24:02/03/04/+ A*11:01/02/03//+ B*51:01/02/03/+ B*15:01/04/06/+
25 Lab.ID HLA-DRB1 HLA-DRB3/4/5 HLA-DQB1 HLA-DPB1 HLA-DQA1 22D01-2 DRB1*04:01/02/03/+ DRB1*08:01/02/03/+ DRB4*01:01/02/03/+ - DRB1*04:05/45/57/+ 22D05-2 DRB1*08:03/36 DQB1*04:01 DQB1*06:01 22D06-4 DRB4*01:01/02/03/+ - 22D06-5 DRB1*04:05/45/57/+ 22D06-6 DRB1*08:03/36 22D06-7 DQB1*04:01 DQB1*06:01 22D08-2 DRB1*04:01/03/05/+ DRB1*08:01/03/05/+ DRB4*01:01/02/03/+ - DQB1*04:01/02 22D12-2 DRB1*04:01/03/05/+ DRB1*08:01/03/05/+ DRB4*01:01/02/03/+ - DQB1*04:01/02 22D14-2 DRB1*04:01/02/03/+ DRB1*08:01/02/03/+ DRB4*01:01/02/03/+ - 22D16-2 DRB1*04:05/06/10 DRB1*08:03 DRB4*01:01/02/03/+ - DQB1*04:01/02 22D17-2 DRB1*04:01/03/05/+ DRB1*08:01/03/05/+ DRB4*01:01/02/03/+ DQB1*04:01/02 22D20 DRB1*04:01/03/05/+ DRB1*08:01/03/05/+ DRB4*01:01/02/03/+ - DQB1*04:01/02 - DRB1*04:05 DQB1*06:01/02/03/ + DQB1*06:01/02/03/ + DQB1*06:01/02/03/ + DQB1*06:01/02/03/ + 22D21-2 DRB1*08:03 22D23-2 DRB1*04:01/03/05/+ DRB1*08:01/03/05/+ DRB4*01:01/02/03/+ DQB1*04:01/02 22D30-2 DRB1*04:01/03/05/+ DRB1*08:01/03/05/+ DRB4*01:01/02/03/+ - DQB1*04:01/02 DQB1*06:01/02/03/ + DQB1*06:01/02/03/ + DPB1*02:01 DPB1*04:02 22D34 DQA1*01:03 DQA1*03:03 22D47-2 DRB1*04:01/03/05/+ DRB1*08:01/03/05/+ DRB4*01:01/02/03/+ - DQB1*04:01/02 22D48-2 DR4 DR8 DQ4 DQ6 22D51-2 DR4 DR8 DR53 - DQ4 DQ6 22D52-2 DRB1*04:01/03/05/+ DRB1*08:01/03/05/+ DRB4 - DQB1*06:01/02/03/ +
26 Lab.ID HLA-DRB1 HLA-DRB3/4/5 HLA-DQB1 HLA-DPB1 HLA-DQA1 22D01-2 DRB1*08:01/02/03/+ DRB1*14:02/03/05/+ DRB3*01/02/03-22D05-2 DRB1*08:02 DRB1*14:05/56 DQB1*03:02:01 DQB1*05:03:01 22D06-4 DRB3*01:01/02/03/+ - 22D06-5 DRB1*08:02 22D06-6 DRB1*14:05 22D06-7 DQB1*05:03 DQB1*03:02 22D08-2 DRB1*08:02/04/13/+ DRB1*14:01/05/06/+ DRB3*01:01/02:01/+ - DQB1*03:02/05/07/+ DQB1*05:01/02/03/+ 22D12-2 DRB1*08:02/04/13/+ DRB1*14:01/02/05/+ DRB3*01/02/03 - DQB1*05:01/02/03/+ DQB1*03:02/05/07/+ 22D14-2 DRB1*08:02 DRB1*14:05/45/56 DRB3*02:02/23-22D14-3 DRB1*08:02 DRB1*14:05/45/56 DRB3*02:02/23-22D16-2 DRB1*08:02 DRB1*14:01/02/05/+ DRB3*01:01/02:01/+ - DQB1*03:02/05/07/+ DQB1*05:01/02/03/+ 22D17-2 DRB1*08:02/04/13/+ DRB1*14:01/02/05/+ DRB3*01:01/02:01/+ DQB1*03:02/05/07/+ DQB1*05:01/02/03/+ 22D20 DRB1*08:02/04/13/+ DRB1*14:01/02/05/+ DRB3*02:01/02/03/+ - DQB1*05:01/02/03/+ DQB1*03:02/05/07/+ DRB1*08:02 22D21-2 DRB1*14:05 DQB1*03:02 DPB1*04:02 DPB1*05:01 22D23-2 DRB1*08:02/04/13/+ DRB1*14:01/02/05/+ DRB3*01/02/03 DQB1*05:01/02/03/+ DQB1*03:02/05/07/+ 22D30-2 DRB1*08:02/04/13/+ DRB1*14:01/02/05/+ DRB3*01/02/03 - DQB1*05:01/02/03/+ DQB1*03:02/05/07/+ 22D34 22D47-2 DRB1*08:02/04/13/+ DRB1*14:01/02/05/+ DRB3*02:01/02/03/+ - DQB1*03:02/05/07/+ DQB1*05:01/02/03/+ 22D48-2 DR8 DR14 DQ5 DQ8 22D51-2 DR8 DR14 DR52 - DQ5 DQ8 22D52-2 DRB1*08:02/04/09//+ DRB1*14:01/02/05//+ DRB3 - DQA1*01:05 DQA1*03:01:0 1
27 Lab.ID HLA-DRB1 HLA-DRB3/4/5 HLA-DQB1 HLA-DPB1 HLA-DQA1 22D01-2 DRB1*04:01/02/03/+ - DRB4*01:01/02/03/+ - 22D05-2 DRB1*04:05/45/57/+ DRB1*04:10 DQB1*04:01 DQB1*04:02 22D06-4 DRB4*01:01/02/03/+ - 22D06-5 DRB1*04:05/45/57/+ DRB1*04:10 22D06-7 DQB1*04:01 DQB1*04:02 22D08-2 DRB1*04:01/03/05/+ - DRB4*01:01/02/03/+ - DQB1*04:01/02-22D10-2 DRB1*04:05/45/57/+ DRB1*04:10 22D12-2 DRB1*04:01/03/05/+ - DRB4*01:01/02/03/+ - DQB1*04:01/02-22D14-2 DRB1*04:01/02/03/+ - DRB4*01:01/02/03/+ - 22D16-2 DRB1*04:05/06/10 - DRB4*01:01/02/03/+ - DQB1*04:01/02-22D17-2 DRB1*04:01/03/05/+ - DRB4*01:01/02/03/+ - DQB1*04:01/02-22D20 DRB1*04:01/03/05/+ - DRB4*01:01/02/03/+ - DQB1*04:01/02 - DRB1*04:05 DRB1*04:10 22D21-2 DQB1*04:01 DQB1*04:02 DPB1*02:01 DPB1*04:02 22D23-2 DRB1*04:01/03/05/+ - DRB4*01:01/02/03/+ DQB1*04:01/02-22D30-2 DRB1*04:01/03/05/+ - DRB4*01:01/02/03/+ - DQB1*04:01/02-22D34 DQA1*03:03-22D47-2 DRB1*04:01/03/05/+ - DRB4*01:01/02/03/+ - DQB1*04:01/02-22D48-2 DR4 DR4 DQ4 DQ4 22D51-2 DR4 - DR53 - DQ4-22D52-2 DRB1*04:01/03/05/+ - DRB4 -
28 Lab.ID HLA-DRB1 HLA-DRB3/4/5 HLA-DQB1 HLA-DPB1 HLA-DQA1 22D01-2 DRB1*04:01/02/03/+ DRB1*14:02/03/05/+ DRB3*01/02/03 DRB4*01:01/02/03/+ DRB1*04:03/06/60/+ 22D05-2 DRB1*14:03 DQB1*03:01/21/22/+ DQB1*03:02 22D06-4 DRB3*01:01/02/03/+ DRB4*01:01/02/03/+ 22D06-5 DRB1*04:06 22D06-6 DRB1*14:03 22D06-7 DQB1*03:01/21/22/+ DQB1*03:02 22D08-2 DRB1*04:01/03/05/+ DRB1*14:03/12/40 DRB3*01:01/02:01/+ DRB4*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*03:02/05/07/+ 22D12-2 DRB1*04:01/03/05/+ DRB1*14:03/12/40 DRB3*01/02/03 DRB4*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*03:02/05/07/+ 22D14-2 DRB1*04:01/02/03/+ DRB1*14:02/03/05/+ DRB3*01/02/03 DRB4*01:01/02/03/+ 22D16-2 DRB1*04:05/06/10 DRB1*14:03/12 DRB3*01:01/02:01/+ DRB4*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*03:02/05/07/+ 22D17-2 DRB1*04:01/03/05/+ DRB1*14:03/12/40 DRB3*01:01/02:01/+ DRB4*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*03:02/05/07/+ 22D20 DRB1*04:01/03/05/+ DRB1*14:03/12/40 DRB4*01:01/02/03/+ DRB3*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*03:02/05/07/+ DRB1*04:06 22D21-2 DQB1*03:01 DQB1*03:02 22D23-2 DRB1*04:01/03/05/+ DRB1*14:03/12 DRB3*01/02/03 DRB4*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*03:02/05/07/+ DPB1*02:0 1 DPB1*05:01 22D30-2 DRB1*04:01/03/05/+ DRB1*14:03/12/40 DRB3*01/02/03 DRB4*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*03:02/05/07/+ 22D34 DQA1*03:01:01 DQA1*05:03 22D47-2 DRB1*04:01/03/05/+ DRB1*14:03/12/40 DRB4*01:01/02/03/+ DRB3*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*03:02/05/07/+ 22D48-2 DR4 DR14 DQ7 DQ8 22D51-2 DR4 DR14 DR52 DR53 DQ7 DQ8 22D52-2 DRB1*04:01/03/05/+ DRB1*14:03/12/40/+ DRB3 DRB4
29 Sample Lab.No 方法 結果 正解 アリル表記 HLA 型アリル表記 HLA 型 # D20 SSP - - DQB1*06:01 DQ6 # D47 SSP C*08:01/02/03/+ Cw6 Cw*0801 Cw8
スライド 1
県立広島病院輸血部 藤井明美 その他 造血幹移植 臓器移植 輸血関連 SSP 法のみ その他 造血幹移植 臓器移植 輸血関連 SSP 法のみ 参加部門 a b c d 参加部門 a b c d No. DNA-QC Lab. No. 使用試薬 No. DNA-QC Lab. No. 使用試薬 1 25D04 H-1,H-2 2 25D06 L-1 3 25D10 L-4 4 25D11 L-2 5
DynalTransplantDiagnostic.indd
2011 1 SeCore HLA SBT 1 2 AllSet + Gold SSP HLA 7 3 SSP UniTray HLA 11 4 27 5 32 ii 1 SeCore HLA SBT SeCore 2 SeCore HLA SBT SeCore 3 SeCore 4 HLA-I 5 HLA-II 5 utype 5 GSSP 6 1 SeCore HLA SBT Sequencing
MHC 2013; 20 ( 2 QC QC DNA-QC 3. QCWS 集会参加および参加証明書発行 QCWS QCWS QCWS 解析方法 DNA-QC Luminex SSO SSO SSP SBT QC FlowPRA Lab Screen WAK Flow ICFA 4 HL
Major Histocompatibility Complex 2013; 20 (: 13 16 QCWS # 1. ワークショップの経過 24 1 QCWS HP QCWS 24 2 57 DNA-QC 53 QC 38 1 20 QCWS DNA-QC QC 4 QCWS 4 4 4 16 QCWS 5 21 57 DNA-QC 53 QC 37 5 6 7 HP 8 25 HP 2. QCWS
スライド 1
DNAタイピング結果解析 HP掲載用 試料説明 日本赤十字社 九州ブロック血液センター 黒田 ゆかり 22nd DNA-QCサンプル 購入した細胞から抽出したDNAを4サンプル配布 ルミネックスSSO法用の陰性コントロール DNase free water を配布 配布サンプル Sample ID (Cap Color) a.dna Label Color H31 H32 H33 Volume Label
スライド 1
Flow PRA 法の検査状況の解析 福岡赤十字病院 検査部 移植検査課 / 輸血細胞治療部 金本人美 参加施設数 -Flow PRA- Screening ClassⅠ 25 施設 ClassⅡ 24 施設 Single antigen ClassⅠ 2 施設 ClassⅡ 2 施設 18th 17th Screening ClassⅠ 22 施設 ClassⅡ 20 施設 Single antigen
MHC23-3
Major Histocompatibility Complex 2016; 23 (3): 168 184 1) 2) 3) 4) 5) 1) 6) 7) 8) 1) 2) 3) 4) HLA 5) 6) 7) 8) HLA 11 25 28 10 22 1 4 61 44 6 1 10 11 5 HLA 60 3 27 3 5 9 5 10 19 5 50 22.4 5.9 8.2% 78.7%
22 S2-4 教育講演 ( 認定制度講習会 ) 9 月 14( 土 ) 10:00 ~ 12:00 座長 EL-1 EL-2 EL-3 HLA 1,2) 1) 2) HLA HLA 17th QC ワークショップ集会 9 月 14 日 ( 土 ) 13:00 ~ 16:00 タイピング結果解析 1
22 特別講演 Ⅰ 9 月 15 日 ( 日 ) 13:40 ~ 14:40 座長 SL-1 ips 特別講演 Ⅱ( スポンサード ランチ付き ) 9 月 16 日 ( 月 ) 12:00 ~ 13:00 座長 SL-2 HLA シンポジウム Ⅰ 9 月 15 日 ( 日 ) 14:40 ~ 16:40 免疫寛容を誘導する養子免疫療法 : その基礎と臨床応用 座長 S1-1 CREST JST S1-2
スライド 1
抗体検査結果解析 (HP 掲載用 ) その他検査法およびクロスマッチ 日本赤十字社中央血液研究所中島文明 その他検査法 ダイレクト クロスマッチ 定 義 その他抗体検査 FlowPRA, LABScreen, WAKFlow 以外の HLA 抗体検査において SH0~SH04 の 4 サンプルを対象としていること ダイレクト クロスマッチ, FCM, ICFAなどクロスマッチ可能な検査方法において
日本組織適合性学会誌第23巻2号
Major Histocompatibility Complex 2016; 23 (2): 115 122 HLA 1 1) 1) HLA MHC 1900 HLA HLA キーワード : HLA HLA HLA HLA 2 HLA HLA HLA 3 1 1 HLA HLA 図 1 2016 6 30 2016 8 2 105 0013 1 10 14 3 5 TEL: 03 5776 0048
スライド 1
部門別解析および結果評価 - 抗体検査 - 日本赤十字社近畿ブロック血液センター高陽淑 参加状況 1. 参加施設の構成 2. 抗体検査実施状況 報告の内容 抗体検査の結果評価 1. 部門別総合判定結果 ( 抗体検出 特異性同定 ) 2. 方法間 施設間の結果比較 3. 部門別評価結果 ( 抗体検出 特異性同定 ) まとめ 1. 17thQCWS 抗体検査に関する講評 2. 18thQCWSに向けて
2 3
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09030549_001.図書館31-1
vol.31 NO.1 2 3 5 6 10 12 8 1947-1954- 1950-1838-1904 1952-1996 1 913-1954 1981-1958- 1 902-1992 1972-1946- 1 940- 1 2 3 3 5 6 6 8 8 12 8 8 1 2 2 http://library.hokkai-s-u.ac.jp/cgi-bin/tosyokan/index.cgi
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