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1 L
2
3 % 3 30 p
4 2
5 OH 5000 p.4, , 3
6 4
7 R1=OH,R2=OH,R3=OH, 配糖体 = ブドウ糖 : デルフィニジングルコシド R1=OH,R2=OH,R3=H, 配糖体 = ブドウ糖 : シアニジングルコシド R1=OH,R2=OH,R3 =OCH 3, 配糖体 = ブドウ糖 : ペチュニジングルコシド R1= OCH 3,R2=OH,R3=H, 配糖体 = ブドウ糖 : ぺオニジングルコシド R1= OCH 3,R2=OH,R3 =OCH 3, 配糖体 = ブドウ糖 : マルビジングルコシド 5
8 DNA SOD OH, 100mg ng/ml 1% - E C
9 FDA Vol.34No.6p Laura BravoNutrition ReviewsVol.56N0.11p No.216p New Food industoryvol.40no.10p p No.264p FRAGRANCE JOURNALVol.25No.4p New Food industoryvol.41no.7p Vol.31No.2p Vol.73No.5p p
10 30 C () 100ml 100g Folin-Ciocalteu 660nm (+)- 1520% 100ml 5585mg 1.5 8
11 mg/100ml ml mg 100g 50540mg g mg 100g mg 1750mg/100g 850mg/100g g
12 mg 100g mg 2000mg/100g 2700mg/100g 1300mg/100g g mg 100ml mg mg/100ml 350mg/100ml 3 250mg/100ml 250mg/100ml ml mg 1 100ml 50320mg 100% mg/100ml ml %450mg 5 10
13 g 50mg ml 205mg C 47mg/100ml ml 370mg mg mg 3050mg/100g - - C HPLC C mg/100g 5.5 mg/100g029 mg/100g 4 7mg/100g 11
14 4565% HPLC 5 12
15 HPLC HPLC HPLC HPLC HPLC % % % 11 HPLC HPLC 1 13
16 mg/100ml mg/100g mg/100ml 3 8 HPLC HPLC mg/100g mg/100g 14
17 % %1 2529g g g g 30 HPLC 9 15
18
19 % % % g g g g 0.35g/kg mg/100ml g/kg 17
20 O SOSA SOD SOD SOSA units/g units/g units/g
21 2300units/g units/g %770units/g 60 19
22 190units/g units/g SOSA SOSA SOSA SOD SOSA SOSA unit/g 20
23 21
24 TBA IC50g ml/ml50% Inhibitory concentration 12 1ml g ml log(ic50) 30 2 log(ic50)
25 %1.59 log(ic50) SOSA TBA C E TBA
26 13 TBA
27
28 26
29 10% % &
30 100% & 20% ウ 28
31
32
33
34 g ml 32
35 30 33
36 34
37
38 % 51.2% 85.8% % % 36
39 66.6% 33.4% % 13.5% 41.3% 43.6%36.1% 39.0% 81.7%41.5% % 40.2% 37.8% 37
40 % 43.1% 21.3% 14.0% 8.4% 38
41 % 37.5%22.3%20.5% %10.7% %35.1%
42 39.5%20.2% 6 3.6%24.5% 5.3% 38.9%59.6% 20 40
43 % 10% 42.0%17.2% 15.8%13.1%
44 g3000mg 100g 50mg 100ml 205mg 100g g 3000mg mg/100ml mg/100g 42
45 1 340ml mg5 1625g 15110mg mg/100g mg/100g g mg1 1323g mg mg/100ml mg/100ml mg/100ml 1 180ml mg ml 90450mg 10105mg/100g 50mg/100g 2 205mg/100ml 1 400g ml mg % % g g % % g g 43
46 mg/100g mg/100g mg/100g mg/100g 1g 1g 1403units/g1350units/g 1303units/g907units/g 44
47 % g ml 45
48 % 33.4% 81.7%41.5% % %37.8% 1.4% 46
49 43.8% 43.1% 14.0% 8.4% % 35.0% 37.5%22.3%20.5% 5.3%38.9% 59.6%31.9% 22.2% 35.2%14.5% 50.6% 30.3%5.4% 2.2% 47
50 48
51 49
52 11 Folin-Ciocalteu Folin-Ciocalteu FC (+)- 1ml 5ml 10ml 1g 50% 8ml 30 10ml 0.5g 2ml 50% 30ml 30 50% 50ml 5g 50% 5ml 50% 5ml % 50ml mg/ml 1ml2 FC 0.5ml0.4M Na2CO3 5ml nm 50% 1ml mg/ml (+)- 50% 1ml FC F-L- 50
53 HPLC 10ml 40mM 40% ph310ml 1 20mM 20% ph320ml 0.45m HPLC 1g 7ml 30 10ml HPLC HPLC TSK-gelODS 80TS4.6mm250mm 20mM ph3.0 20mM 40% ph nm 1.0ml/min4010l (+)- (-)-(-)-(-)- (-)- (-)- HPLC HPLC 30 51
54 F- 5, N-(DMPO)(ESR) (SOD) SOD unit/g 515g 0.1M ph7.8 10ml 5g 0.1M ph ml 60 1g Tween80 1g 0.1M ph M ph ml 1g 0.1M ph7.850ml g 0.1M ph M ph ml M 5,5--1--N-(DMPO)15l 9.5mM -N,N,N,N,N -(DETAPAC)- 35l 2mM -50l SOD 50l 0.4units/ml -50l 160lESR 40 Mn 2+ 52
55 ESR JES-FR80 8mW335.2mT5mT100kHz mT TBA 30 5ml 20ml 25ml 1g 80% 5ml % 25ml 1g 80% 5ml 80% 10ml 30 80% 10ml % 50ml (+)-10mg 80% 10ml mg/ml 10ml 1ml1.25mg/ml 1%SDS 0.25ml % BHT2,6-0.25ml 0.4% TBA 0.2M (ph3) 1ml ml n- (150:10) 4ml 3000rpm20 532nm IC5050% 53
56 IC g/ml 0.1ml0.1mg/ml- 0.25ml20%w/v Tween40 0.5ml 45ml 0.2M ph6.85ml - 0.1ml 4.9ml - 470nm TBA IC50 54
57 CM 10 55
58 mg
59 57
60 58
61 59
62 60
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g 51 52
10 12 A 12 C 12 13 13 13 13 16 16 18 18 18 23 23 23 23 23 A 24 25 25 26 28 28 30 32 38 38 41 42 43 10 43 11 45 48 48 120g 51 52 12 3 12 3 350g 120g 1 7 10 260g 90g 90g 30g 9 11 2 gg1 g 1 120g 10 1 12 6
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24 11 10 24 12 10 30 1 0.45m1.00m 1.00m 1.00m 0.33m 0.33m 0.33m 0.45m 1.00m 2 23% 29% 71% 67% 6% 4% n=1525 n=1137 6% +6% -4% -2% 21% 30% 5% 35% 6% 6% 11% 40% 37% 36 172 166 371 213 226 177 54 382 704 216
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1 3 6 7 8 3 6 7 8 g mg 6 1 16 16 18 16 13 138 13 16 1 11 8 78 8 17 9 9 g mg 18 13 1 1 1 99 6 3 36 3 3 8 1 18 18 18 16 16 1 1 g mg 1 1 13 13 1 11 11 g mg 8 6 g 1 g mg 93 38 9 93 8 8 176 1 1 1 1 11 18
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1 2 3 4 5 6 7 1 2 3 4 5 6 7 2 2 2 1 1 2 9 10 11 12 13 14 15 16 17 1 8 2 3 4 5 6 7 1 2 3 4 5 6 7 8 9 10 9 11 12 13 13 14 15 16 17 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 1 2 3 4 5 6 7 8 9 11 12
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1 1 1 2 3 4 5 6 7 8 9 e e 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 10mm 150mm 60mm 25mm 40mm 30mm 25 26 27 1 28 29 30 31 32 e e e e e e 33 e 34 35 35 e e e e 36 37 38 38 e e 39 e 1 40 e 41 e 42 43
1 1 36 223 42 14 92 4 3 2 1 4 3 4 3429 13536 5 6 7 8 9 2.4m/ (M) (M) (M) (M) (M) 6.67.3 6.57.2 6.97.6 7.27.8 8.4 5 6 5 6 5 5 74 1,239 0 30 21 ( ) 1,639 3,898 0 1,084 887 2 5 0 2 2 4 22 1 3 1 ( :) 426 1500
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第18回海岸シンポジウム報告書
2011.6.25 2011.6.26 L1 2011.6.27 L2 2011.7.6 2011.12.7 2011.10-12 2011.9-10 2012.3.9 23 2012.4, 2013.8.30 2012.6.13 2013.9 2011.7-2011.12-2012.4 2011.12.27 2013.9 1m30 1 2 3 4 5 6 m 5.0m 2.0m -5.0m 1.0m
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