22 2013 24514 hcg500 IU/kg4554 16 Nested-PCRVNN 2426 0.5 ppm1 90.9 80.9 SAI54.2±0.2 0.5 m 3 211 5,000 95.214,286/2 6 3 57 9 9 010 031 m 2 0.1 ml 30 330 7 mm S S340 2024 6 mm25 37 1040 49X 192X 192X 1 14 2 0 46.7% 11.1±8.2%2 06.7% 2.3±2.5%0.9±1.9%21819 2025314049 P < 0.05 98
P < 0.05192 2 2013 24 % ±SD 10,162±2,322 71.1±16.3 778±362 5.5±2.5 10,566±2,480 74.0±17.4 571±239 4.0±1.7 7,578±2,764 53.0±19.4 529±152 3.7±1.1 ±SD 10 192 606729.6 mm 11.3 cm % % ±SD ±SD % % 2.3±2.5 b 11.3±9.5 100 9.3 0.9±1.9 b 11.6±5.5 100 14.8 11.1±8.2 a 3.5±2.9 b ) Tukey-Kramer P < 0.05 606729.6 mm ±SD % ±SD 100 5.6 100 1.9 99
3 730g2.5 2.5 2.5m 2 78 6%6% 12%12%MP 2 3 1 1 1. 1 1 2 69122 6 3 TPU-2C2 0h 24h 48h 72h 168h 1cm 8 2.5mm/sec 90% 4K 25g 0h24h48h72h168h -80 QS-SOLUTION K 7-33 7 1. 1 2012 6 7 2013 2 26 2m 12.828.0 123 2m 15.027.721.39 1 1. 2. 2. SD 100
2 3. 4 5 9 2 6% 12% 6%12% 12 6% 12% 3 5. 12 168h 6%12% 6 4. 6. 12 4K K 2. 1 3 7 6 6% 20 15 6 6% 101
1. 4 1 10kg/ m3以下 30 18 500L 2 1 1 280L 30% 30% 50% 30% 1% 2% 50%30%30%+T130%+T2 g 300 180 180 180 g 150 190 190 190 g 080 130 130 130 g 070 100 100 100 g 006 012 g 600 600 606 612 1 1. 1kg 50%145 30%121 30%+T1 123 30%+T2 126 2 7 Edwardsiella tarda MEE0309 10 7 CFU/mL 1.5 100L 24 PBS TSA2548 TSA 1ml 1g 3 7 9 NBT PK 4 10% HE 100% 2. 222m 2 2.5 1.1,085 10.7gEP 35 1 1 35 1 HOBO onset U20-001-01-Ti1 2 1 60 3Edwardsiella tarda 2012 9 18 2013 1 21 30 E. tarda 9 18 NBT PK 4 1 21 6 E. tarda 1. 49.4g 102
96.4g 127.0g 2012 8 3 10 3 61 10 16 12 11 58 2013 1 7 3 6 58 26.920.013.5 1 2 30% +T1 50% 50% 30% 30%+T1 30%+T2 (%) 124.6±25.6 112.3±2.7 118.2±20.3 117.8±3.5 (%) 27.6±4.5 26.6±0.8 27.5±5.0 28.5±1.2 (%) 31.7±2.2 31.1±0.4 32.3±5.5 31.5±1.0 (%) 13.5±0.3 13.3±0.6 14.2±2.4 13.6±0.3 (%) 7.9±1.9 3.8±0.1 2.8±1.2 4.4±3.9 (%) 11.5±3.4 6.0±0.1 4.6±2.3 6.8±5.8 2 2. 3 NBT PK 3030%+T2 30%+T2 30%+T1 3 50% 30% 30%+T1 30%+T2 0.009±0.022 0.029±0.027 0.016±0.020 0.004±0.014 0.021±0.028 0.008±0.013 0.014±0.024 0.006±0.007 0.023±0.011 0.022±0.011 0.015±0.012 0.034±0.019 4 3. PK 30%+T1 30%+T2 30% 50%30% +T2 30% 50%30%+T2 4 4. 50% 30% 30%+T1 30%+T2 (μm) 107.6±40.6 81.7±15.2 129.9±37.3 125.4±24.8 (%) 564.2±122.5 515.8±152.5 452.9±126.9 461.7±106.1 (μm) 115.2±33.9 88.5±20.5 99.1±15.9 112.9±17.8 (%) 715.7±145.2 590.6±122.9 619.1±144.4 544.6±185.1 (μm) 106.7±27.8 97.8±25.4 107.6±29.5 110.6±33.8 2. 2012 6 26 2013 1 21 2m 13.328.0 1 2.43 0.65kPa3.381.37kPa 2 8 77.7%74.1% 5 (%) (%) 5. 7 8 9 10 11 12 1 00.0 40.0 80.0 93.3 98.3 100.0 98.3 01.7 65.0 88.3 91.7 96.7 100.0 98.3 01.7 15.0 11.7 08.3 11.7 006.7 10.0 01.7 13.3 05.0 10.0 08.3 008.3 13.3 3Edwardsiella tarda NBT 6PK 4 6. NBT 9 0.068±0.044 0.050±0.014 1998. 9. 4-12 103
6 1 MP 0.1 2% MP 2 333m 2 51 1,476g3 1 1 2012 5 28 7 23 8 2m 20.424.6 0 2 4 8 6 2 333m 2 38 1,926g 3 1 1 2012 10 22 12 17 8 2m 24.5 15.80 2 4 8 8 1 1. 1 (5 28 7 23 ) 4 2 2 0h48h96h168h 8 L C3 104
2. 4 0h48h96h168h 8 L C 6 3. 8 4 168h 16.3 4 4 4 8 6. 8 4 8 4. K 4 8 5 5. 2 (5 28 7 23 ) 105
1)1 1 360 1.4g 30L 8 24 6 20 1 44 8 4 4 2 3 1 2g 2 8 20 10 24 12 25 2 26 2)0 24 11 13 0 0.5g1,500 30L 12 1 126 12 4 4 4 3 1 1g 2 1 22 3 14 2006 1)1 13.926.9 13.916.210 24 38.1 67.3112.8 254.315 1 12 2)0 1 2 13.720.3 3 14 41.3 34.5 55.2 27.6mm 25.2mm28.1mm 2 106
2 2 1 MP MP 2%MP 2%MP 3 500L 3 25 137g3 1 1 2013 1 21 3 18 8 1 250L 0 4 8 6 9 NBTPK PK 11.8 14.6 107 1 1 2 L a ()b C 2 4 NBT PK 3 NBT 34
26 3 517-0404 3564-3 TEL 0599-53-0016 FAX 0599-53-2225 E-mail: suigi@pref.mie.jp http://www.mpstpc.pref.mie.lg.jp/sui/