V Q v V 1000 VE 1 C DL E = DL l l l l
V Q v V 1000 VE 1 C DL E = DL l l l l
l l l l
2
V Q k v V 1000 VE 1 C DL E = DL l l l l l l l 0.488 0.253 4 50 V = 1000 10 1 0.015 0.17
W Q k v V 1000 VE 1 C DL E = DL l l l l 0.488 0.336 4 50 1 W = 0.013 1000 25 0.1
l
l
l
l
l l l l l l l l l l l l l l l l l l l l l
l l
l l l l l l
l l l l l l l l l l l l
l l l l l
l l l l l
l l l l l l l l l l
l l l l l l l l l l l l l
l l l l l l (ml) (cells/ml) (ml) = 5 7.5 10 (cells/ml) l l l l l l l l l l l l
l l l l l l l
l l l l
l l [ S] [ S] Vmax V = + b K m +
V = n Vmax [ S] n [ K ] + [ S] m n + b 2,3,7,8-TeCDD - (pg/well) (pg/ml) 25000 A B= C= RLU A/1901000 ln(b100) 20000 23.75 125.00 9.43 22880 11.9 62.5 8.74 19448 5.94 31.3 8.05 16471 15000 2.97 15.6 7.35 12357 1.48 7.81 6.66 7517 10000 0.742 3.91 5.97 4754 0.371 1.95 5.27 2114 0.186 0.977 4.58 1233 5000 0.0928 0.488 3.89 434 0 Relative Light Unit 3.0 5.0 7.0 9.0 11.0 V max = 28631 n= 6.59 [2,3,7,8-TeCDD]C=ln(B100) K m = 7.75 b= 192 ( b) RLU Km 1 ( V max RLU b n = )n
l e = 100 l l 100 = 3 (ng/g ng/m N ) = 3 (g m N ) 21 On C = Cs 21 Os l l
l l l
C DL QDL k v VE 1 = C 1000 VE V QL QQL k v VE 1 = 1000 VE V l l l l l l l l l l l l l l C DL QDL k v VE 1 QQL k v VE 1 = C QL= 1000 VE W 1000 VE W
l l l l l l l l l l l l l l 20 80 80 y = 0.253x y = 0.351x y = 0.336x 15 R 2 60 = 0.994 R 2 60 = 0.940 R 2 = 0.820 (n=46) (n=50) (n=44) 10 40 40 5 20 20 0 0 0 0 20 40 60 80 0 50 100 150 0 50 100 150 (ng/m 3 N) (ng/g) (ng/g) (ng-teq/m 3 N) (ng-teq/g) (ng-teq/g)
V Q k v V 1000 VE 1 C DL E = DL l l l l l l l 300 0.217 0.08 10 1 V = 0.061 1000 5 0.17
W Q k v V 1000 VE 1 C DL E = DL l l l l l l l 300 0.217 0.2 10 1 W = 0.13 1000 10 0.1
l l l
l
l l l l l l l l l l l l l l l
l l l l l l
l l l l
l l l l l l l l l l l l l
l l l l
l l l l l l l
l 21 On C = Cs 21 Os l
V Q k v V 1000 VE 1 C DL E = DL l l l l l l l 100 0.375 0.07 50 1 V = 0.031 1000 25 0.17 W Q k v V 1000 VE 1 C DL E = DL l l
l l l l W 100 0.872 0.07 50 1 = 0.061 1000 50 0.1
l l l l l
l
l l
l
l
l l l l l l
l l l l l l l l l l l l l l
l l l l l l l l l l
l l l
2 i i i s s 2 2 i 2 i w = s ( s n) i i y = + + - x 1 * e l
1 ln -1 y- X = 10 VE 1 Cs = X n v VE V l l l l 21 On C = Cs 21 Os C w VE 1 = X n v VE W l l l l
V Q v V 1000 VE 1 C DL E = DL l l l l l l l 10 1 20 1 V = 0.12 1000 10 0.17 W Q v V 1000 VE 1 C DL E = DL l l l l
l l l W 3 1 20 1 = 0.060 1000 10 0.1
l
l l l
V ' E = C 1 v V E C 2 1 x 1000 l l l l l l l 1 V ' E = 250 1 20 = 1 1 5 1000 l l l l l l
l l l l l l l l l l l
l l l l l l l l
l l l l l l l l l l l l l l l l ll l l l l l
l l l l
( AD ) y = D x B 1 C l l 3 (ng/ml) (ml) 1 (ng/m N ng/g) = 3 (ml) (ml) (m N g) 21 On C = Cs 21 Os
l l l l l l l l l l Q = 0.0744 1000 3 ( )( ng - TEQ/m N ) l l 0.9779 0.8351 Q ( )( ng - TEQ/g) = 0.0038 1000 l l
Q ( )( ng - TEQ/g) = 0.0038 1000 0.9779 l ll l l ll l l ll l l ll l l ll l l ll l
l l 100 10 1 0.1 0.01 0.001 n=29 y = 0.0744(x/1000 0.8351 R2 = 0.979 0.0001 1 10 100 1000 10000 100000 1E+06 l l 100 10 1 0.1 n=12 y = 0.0038(x/1000 0.9779 R2 = 0.988 0.01 1000 10000 100000 1000000 10000000