Title ゾウリムシの生物対流実験 ( 複雑流体の数理とその応用 ) Author(s) 狐崎, 創 ; 小森, 理絵 ; 春本, 晃江 Citation 数理解析研究所講究録 (2006), 1472: 129-138 Issue Date 2006-02 URL http://hdl.handle.net/2433/48126 Right Type Departmental Bulletin Paper Textversion publisher Kyoto University
1472 2006 129-138 129 (So Kitsunezaki) Graduate School of Human Culture, Nara Women s Univiversity (Rie Komori) (Terue Harumoto) $\dot{\mathrm{x}}^{\mathrm{l}}\mathrm{j}\backslash$ Hele-Shaw cell Pammecium tetraurelia ( ) P. tetraurelia $\mathrm{x}^{\iota}1\mathrm{l}$ 1 Introduction $\neg \mathrm{x}\backslash$ (bioconvection) Rayleigh- B\ enard (RB) $Tetrahymena_{\backslash }$ $Chlamydomonas_{\text{ }}$ Bacillus subtdis $[1, 2, 3, 4]_{\text{ }}$ (1) (2) Rayleigh-Taylor(RT) $[5, 6, 7]_{0}$ $\text{ }$ oxygentaxis( ) phototaxis( ) gyrotaxis geotaxis ( ) Navie Stokes $\mathrm{r}\mathrm{b}$ $[8, 9, 10, 11, 12, 13]_{0}$ $[14]_{\text{ }}$ $\neg \mathrm{x}\backslash$ $\mathrm{x}\gamma_{\backslash P. tetraurelia }$ Hele-Shaw cell 2 $\urcorner \mathrm{x}\backslash$ $\mathrm{x}\neg\backslash$
stigmasterol 130 thermotaxis, glavanotaxis, chemotaxis, oygentaxis, rheotaxis $[15, 16, 17]_{\text{ }}$ gyrotaxis $\mathrm{x}^{\iota}1[perp]$ P. tetmurelia 2 P. tetmurelia wild-type, stock 51 $(15^{\mathrm{o}}C)$ $(\mathrm{f}\mathrm{f}\mathrm{l}\backslash 400\mathrm{m}1)$ 1 $25^{\mathrm{o}}C$ 3\sim 4 (stationary phase) $10^{3}\mathrm{c}\mathrm{e}11\mathrm{s}/\mathrm{m}1$ $\langle$ SMBIII[20] $1\mathrm{m}1$ $10^{5}\mathrm{c}\mathrm{e}11\mathrm{s}/\mathrm{m}1$ 1/40 $5\mu l$ 15 $n_{0}$ 2 Hele-Shaw cell $W=66.7\pm 0.1mm_{\text{ }}$ $D=1.23\pm 0.05mm$ $H$ $V$ $n_{0}$ SMBIII $26\sim 28^{\text{ }}C$ Hele-Shaw cell $\mathrm{x}^{\backslash }1\mathrm{A}$ (Canon EOSIOD) (wraymer SW-700TD) 3 $P$. tetraurelia $\mathrm{x}\backslash [[perp]_{-}$ $V=1\mathrm{O}\mathrm{O}\mathrm{O}\mu l$ 1 10.5% phosphate-buffered wheat grass powder ( Pines Int., Inc., Lawrence, $\mathrm{k}\mathrm{s}$) infusion supplemented $\mathrm{m}\mathrm{g}/1$ with 0.5 and inoculated with Enterobacter aerogenes 2-3 days before use $[18, 19]$.
$[perp]\backslash$ 131 $\mathrm{x}\urcorner\backslash$ 1: ( ) $V=1000\mu l$. ( )no, ( )n0 $=0.61\pm 0.22\mathrm{x}10^{5}\mathrm{c}\mathrm{e}11\mathrm{s}/\mathrm{m}1$ $=0.21\pm 0.03\mathrm{x}10^{5}\mathrm{c}.\mathrm{e}11\mathrm{s}/\mathrm{m}1$ $\mathrm{x}^{\mathrm{t}}1[perp]$ $\mathrm{x}^{\backslash $\mathrm{x}^{\overline{\backslash }1}[perp]$ P. tetraurelia }1\mathrm{A}_{-}$ ( ) $\mathrm{x}^{\overline{\backslash }1}[perp]$ $n_{0}$ $H$ $\mathrm{x}$] $\langle$ 1 $y$ $\urcorner 1 (space-time image ) \mathrm{x}\backslash$ 2 2 2 space-time image $1000\mu l$ ( ) XL- 1 $500\mu l$ ( )
132 2: space-time images $n_{0}=0.61\pm 0.22\mathrm{x}10^{5}\mathrm{c}\mathrm{e}11\mathrm{s}/\mathrm{m}1$, $V=1\mathrm{O}\mathrm{O}\mathrm{O}\mu l,$ $y=0.75h_{\text{ }}$ $500\mu l,$ $y=0.5h_{\text{ }}$ Harashima. akasiwo $H$ $\neg \mathrm{x}$ $\backslash$ $\mathrm{x}\mathrm{j}[perp]\backslash$ $[14]_{\text{ }}$ $[perp] \mathrm{x}^{\overline{4}}\mathrm{f}$ $\mathrm{r}\mathrm{t}$ $\mathrm{x}^{\overline{\backslash }1}[perp]$ $[5, 6]_{\text{ }}$ 2 $\mathrm{x}\urcorner\backslash$ 4 $\mathrm{x}1[perp]\backslash$ Hele- Shaw cell ( ) $\mathrm{x}^{\overline{\backslash }1}[perp]$ 3 3: $\mathrm{x}1[perp]\backslash$ $(0.3\mathrm{m}\mathrm{m}\phi)_{0}$ $\mathrm{x}^{\iota}1[perp]$ geotaxis ( )
$[perp]\backslash$ 133 4: $\mathrm{x}^{\backslash }1[perp]$ 4 1 Hele-Shaw cell $arrow$ $arrow$ $arrow$ $arrow$ 2 $\mathrm{x}^{\backslash $\urcorner \mathrm{x}\backslash$ }1[perp]$ $\mathrm{x}^{\backslash 1 }1L$ $\mathrm{y}$] $\mathrm{x}\urcorner\backslash$ $\mathrm{y}\mathrm{j}\mathrm{l}\backslash$ $\mathrm{x}\neg\backslash$ ( )
$(\mathrm{m}^{06}\mathrm{n}\vee \mathrm{s})$ 0.8 134 0.3 air 0.2 0.1 $\mathrm{n}_{2}$ $0_{0}$ 0.2 $\mathrm{s}\mathrm{p}^{0.4}\mathrm{e}\mathrm{e}\mathrm{d}$ 1 5: 6: $\mathrm{t}\mathrm{a}$ 5 $22\mathrm{m}\mathrm{m}$ ( ) 1/40 $n_{0}=1.9\pm \mathrm{o}.26\mathrm{x}10^{5}\mathrm{c}\mathrm{e}11/\mathrm{m}1$ $2.\mathrm{l}\mathrm{m}\mathrm{m}$ 800\mu 4 5, 6 5 ImageJ 2 1 $0.1\sim 0.2\mathrm{m}\mathrm{m}/\mathrm{s}$ 2 http: $//\mathrm{r}\mathrm{s}\mathrm{b}$.info $\mathrm{n}\mathrm{i}\mathrm{h}.\mathrm{g}\mathrm{o}\mathrm{v}/\mathrm{i}\mathrm{j}/$..
135 7: $3\sim 0.6\mathrm{m}\mathrm{m}/\mathrm{s}$ 0 a 7 1 $\mathrm{t}^{\mathrm{a}}$ 8 50 1 1 50 xl- 1 ( ) ( ) $\mathrm{x}^{\mathrm{t}}1[perp]$ $\mathrm{x}^{\iota}1[perp]$
$1\mathrm{f}\mathrm{f}\mathrm{i}^{\text{ }}\lrcorner]\text{ _{ }\subset l^{\text{ } }}\backslash \text{ ^{ }},/\text{ }\underline{\ovalbox{\tt\small REJECT}}\mathrm{f}\check{\mathrm{f}\mathrm{l}} \mathrm{f}\underline{\mathrm{f}\mathrm{i}}_{1}^{\text{ }}\underline{\ovalbox{\tt\small REJECT}}\not\in\ovalbox{\tt\small REJECT}_{l}\underline{0.1\mathrm{m}\mathrm{m}}. 1t^{\prime\backslash }\vee\sim--1^{\mathrm{i}} - \backslash r^{1},\backslash \grave{\mathrm{t}},$ $\sim$ $\vee$! $ $ $\backslash$ $r^{1}$ $\ovalbox{\tt\small REJECT}_{l^{J }}\backslash _{-} \ovalbox{\tt\small REJECT}^{\prime^{J }}-\backslash -,\nearrow \backslash \prime^{\prime^{\prime^{-\backslash }}\backslash }--\backslash _{\backslash } ^{\wedge^{\prime }} $ $\dot{\mathrm{x}}1$ $\backslash$ 138 1 $t $., $($ $0.1\mathrm{m}\mathrm{m})_{0}$ 8: 1 1 3 ( ) ( ) $\mathrm{x}^{\mathrm{t}}1[perp]$
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