1 SOP
Copyright 2007 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner. eppendorf, Eppendorf Reference, Eppendorf Research, Eppendorf Research plus, Multipette plus, Multipette stream/xstream, Varipette, Varispenser, Biomaster, Combitip and PICASO are registered trademarks of Eppendorf AG, Hamburg, Germany. AESOP13640-/0310
1... 5 1.1... 5 1.1.1... 5 1.1.2... 5 1.1.3... 5 1.2... 6 1.2.1... 6 1.2.2... 6 1.2.3... 6 1.2.4... 6 1.2.5... 6 1.2.6... 6 2... 7 2.1... 7 2.1.1... 7 2.1.2... 7 2.1.3... 7 2.1.4... 7 2.2... 8 2.2.1... 8 2.2.2... 8 2.2.3... 8 3... 9 3.1 Systematic error... 9 3.2 Randam error... 9 4... 10 4.1... 10 4.1.1 4900/4910 4830... 10 4.1.2... 10 4.1.3... 10 4.1.4... 10 4.1.5 4720... 11 4.1.6... 11 4.1.7 Xplorer... 11 4.2... 11 5... 12 5.1... 12 6... 13 6.1... 13 7... 14 7.1... 14 7.2... 14 7.2.1... 15 7.2.2 4830... 15 7.2.3 4910... 16 7.2.4 V 3111... 17 3
7.2.5 V 3120 -... 18 7.2.6 M 3114... 19 7.2.7 M 3122... 20 7.2.8... 20 7.2.9 Xplorer... 20 7.3... 21 7.3.1 4900... 21 7.3.2 F 3112... 22 7.3.3 F 3121... 22 7.4... 23 8 Z... 24 8.1 Z... 24 9... 25 9.1... 25 9.1.1 4900... 25 9.1.2 F 3112... 26 9.1.3 F 3121... 27 9.2... 28 9.2.1 4910... 28 9.2.2 V 3111... 29 9.2.3... 30 9.2.4 V 3120... 31 9.2.5 4830... 32 9.2.6 4720... 32 9.2.7 Xplorer... 33 9.3... 34 9.3.1 M 3114... 34 9.3.2 4860... 35 9.3.3 M 3122... 35 9.3.4 Xplorer... 36 9.4... 37 9.4.1 4981... 37 9.4.2 stream/xstream... 38 9.5... 39 9.5.1... 39 9.5.2... 39 9.6 EN ISO 8655... 40 9.6.1... 40 9.6.2 4830... 41 9.6.3... 41 9.6.4... 42 9.6.5... 42 4
1 1 EN ISO 8655 Volume-measuring equipment with pistons. 1.1 1.1.1 1.1.2 EN ISO 8655 10μL 6 5 1.1.3 * 1μL~10μL >10μL~ ~100μL >100μL~1,000μL >1mL~10mL >10mL~ ~200mL mg 0.001 0.01 0.1 0.1 1 * 5
1 1.2 1.2.1 50μL 1.2.2 PICASO 3113 004.001 EN ISO 8655, Part 6 1.2.3 15 C 30 C 50% 1.2.4 2 1.2.5 ISO 3696 grade 3 1.2.6 6
2 2 2.1 4780 4980/4981 1/5 4985 stream/xstream 2.1.1 3 10 50% 10% 2.1.2 3 10 50% 10% 2.1.3 4780 4980/4981 4985 stream Xstream 10 2.1.4 10 7
2 2.2 2.2.1 1. 2. 3. 4. 3 5. 5 6. 7. 1 2.2.2 1. 2. 2~3mm 3. 1~3 V 1-10 ml V 1-10 ml 5 4. 2.2.3 1. 2. 3. 4. 5. 6. 7. 9 8. 50% 8
3 3 3.1 Systematic error X Z n n = Z 29 Z =Systematic error e s μl e X X Systematic error% e 100 X X X 3.2 Random error n =Random error (s) s X Z X n 1 CV Random error CV % S X 100 30 9
4 4 4.1 121 C 20 1 4.1.1 4900/ 4910 4830 4900/4910 4830 1. 1 2. 4.1.2 1. 2. 5mL 10mL 1 3. 4. 5mL 10mL 4.1.3 1. 2. 4.1.4 10
4 4.1.5 4720 4720 4780 4980/ /4981 4985 stream Xstream 4.1.6 1. 2. 3. 4. 4.1.7 Xplorer Xplorer 1. 2. PUSH UP TO RELEASE 4.2 60% 1. 2. 3. 4. 11
5 5 5.1 1. 2. 20μL 3. 30 4. 12
6 6 6.1 13
7 7 7.1 ISO3696 1 3 12 4830 7 9 7.2 V 3120 M 3122 V 3111 M 3114 4910 4830 14
7 7.2.1 15 30 C ±0.5 C EN ISO 8655, Part 6 V 3120 M 3122 V 3111 M 3114 4910 1. 2. 1 3. 10 4. 5. 9 24 Z 6. 7. 2 6 8. 50% 7.2.2 4830 No. 4910 092.001 1. 2. 3. 15
7 7.2.3 4910 No. 4910 092.001 1. B 2. 3. 4. 16
7 7.2.4 V 3111 No. 3111 501.016 1. D 2. 90 3. 1 0.1-2.5μL 0.5-10μL 2-20μL 10-100μL 20-200μL 100-1,000μL 500-5,000μL 1-10mL 0.1μL 0.5μL 1μL 5μL 10μL 50μL 250μL 510μL 4. 5. 6. 17
7 7.2.5 V 3120-0 No. 3120 633.006 1. 2. 3. 4. 5. 6. 7. 18
7 7.2.6 M 3114 No. 3111 501.016 1. D 2. 90 3. 1 0.5-10μL 10-100μL 30-300μL 0.5μL 5μL 10μL 4. 5. 6. 19
7 7.2.7 M 3122 18 7.2.8 1 1 20 C 7.2.9 Xplorer Xplorer Xplorer 20
7 7.3 14 10 7.3.1 4900 No. 4910 092.001 1. 2. B 3. 14 1 1 2 5 10μL 10 20μL 25 50μL 100μL 200 250μL 500 1,000μL 1,500 2,000 2,500μL 0.5μL 1μL 2.4μL 5μL 12μL 46μL 118μL 21
7 7.3.2 F 3112 No. 3111 501.016 1. D 2. 90 3. 1 10μL 20μL 25μL 50μL 100μL 200μL 250μL 500μL 1,000μL 0.8μL 0.8μL 0.8μL 0.8μL 0.8μL 38μL 38μL 38μL 38μL 7.3.3 F 3121 18 22
7 7.4 14 mg µl 1. μl 2. 3. 4. 5. 23
8 Z 24 8 Z 8.1 Z Z μl/mg EN ISO 8655 C kpa 80 85 90 95 100 101.3 105 15 15.5 1.0017 1.0018 1.0018 1.0019 1.0019 1.0019 1.0019 1.0020 1.0020 1.0020 1.0020 1.0020 1.0020 1.0021 16 16.5 1.0019 1.0020 1.0020 1.0020 1.0020 1.0021 1.0021 1.0021 1.0021 1.0022 1.0021 1.0022 1.0022 1.0022 17 17.5 1.0021 1.0022 1.0021 1.0022 1.0022 1.0023 1.0022 1.0023 1.0023 1.0024 1.0023 1.0024 1.0023 1.0024 18 18.5 1.0022 1.0023 1.0023 1.0024 1.0023 1.0024 1.0024 1.0025 1.0025 1.0025 1.0025 1.0026 1.0025 1.0026 19 19.5 1.0024 1.0025 1.0025 1.0026 1.0025 1.0026 1.0026 1.0027 1.0026 1.0027 1.0027 1.0028 1.0027 1.0028 20 20.5 1.0026 1.0027 1.0027 1.0028 1.0027 1.0028 1.0028 1.0029 1.0028 1.0029 1.0029 1.0030 1.0029 1.0030 21 21.5 1.0028 1.0030 1.0029 1.0030 1.0029 1.0031 1.0030 1.0031 1.0031 1.0032 1.0031 1.0032 1.0031 1.0032 22 22.5 1.0031 1.0032 1.0031 1.0032 1.0032 1.0033 1.0032 1.0033 1.0033 1.0034 1.0033 1.0034 1.0033 1.0034 23 23.5 1.0033 1.0034 1.0033 1.0035 1.0034 1.0035 1.0034 1.0036 1.0035 1.0036 1.0035 1.0036 1.0036 1.0037 24 24.5 1.0035 1.0037 1.0036 1.0037 1.0036 1.0038 1.0037 1.0038 1.0037 1.0039 1.0038 1.0039 1.0038 1.0039 25 25.5 1.0038 1.0039 1.0038 1.0040 1.0039 1.0040 1.0039 1.0041 1.0040 1.0041 1.0040 1.0041 1.0040 1.0042 26 26.5 1.0040 1.0042 1.0041 1.0042 1.0041 1.0043 1.0042 1.0043 1.0042 1.0044 1.0043 1.0044 1.0043 1.0044 27 27.5 1.0043 1.0045 1.0044 1.0045 1.0044 1.0046 1.0045 1.0046 1.0045 1.0047 1.0045 1.0047 1.0046 1.0047 28 28.5 1.0046 1.0047 1.0046 1.0048 1.0047 1.0048 1.0047 1.0049 1.0048 1.0049 1.0048 1.0050 1.0048 1.0050 29 29.5 1.0049 1.0050 1.0049 1.0051 1.0050 1.0051 1.0050 1.0052 1.0051 1.0052 1.0051 1.0052 1.0051 1.0053 30 1.0052 1.0052 1.0053 1.0053 1.0054 1.0054 1.0054
9 9 20~25 C ±0.5 C 10 ISO 8655 9.1 9.1.1 4900 μl 1 2 5 10 10 20 25 50 100 200 250 500 1,000 1,500 2,000 2,500 Systematic error (inaccuracy) % μl 20μL 20μL, L ±2.5 ±0.025 ±2.0 ±0.04 ±1.5 ±0.075 ±1.0 ±0.1 200μL ±1.0 ±0.8 ±0.8 ±0.7 ±0.6 ±0.6 ±0.6 ±0.6 ±0.6 ±0.6 ±0.6 ±0.6 1,000μL 2,500μL ±0.1 ±0.16 ±0.2 ±0.35 ±0.6 ±1.2 ±1.5 ±3 ±6 ±9 ±12 ±15 Random error (imprecision) % μl ±1.8 ±1.2 ±0.8 ±0.5 ±0.5 ±0.3 ±0.3 ±0.3 ±0.2 ±0.2 ±0.2 ±0.2 ±0.2 ±0.2 ±0.2 ±0.2 ±0.018 ±0.024 ±0.04 ±0.05 ±0.05 ±0.06 ±0.075 ±0.15 ±0.2 ±0.4 ±0.5 ±1 ±2 ±3 ±4 ±5 25
9 9.1.2 F 3112 Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 200μL 10 ±1.2 ±0.12 ±0.6 ±0.06 20 ±1.0 ±0.2 ±0.3 ±0.06 25 ±1.0 ±0.25 ±0.3 ±0.075 50 ±0.7 ±0.35 ±0.3 ±0.15 100 ±0.6 ±0.6 ±0.2 ±0.2 1,000μL 200 ±0.6 ±1.2 ±0.2 ±0.4 250 ±0.6 ±1.5 ±0.2 ±0.5 500 ±0.6 ±3 ±0.2 ±1 1,000 ±0.6 ±6 ±0.2 ±2 26
9 9.1.3 F 3121 Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 20μL 40mm 10 ±1.2 ±0.12 ±0.6 ±0.06 20μL, L 46mm 20 ±0.8 ±0.16 ±0.3 ±0.06 200μL 53mm 10 ±1.2 ±0.12 ±0.6 ±0.06 20 ±1.0 ±0.2 ±0.3 ±0.06 25 ±1.0 ±0.25 ±0.3 ±0.075 50 ±0.7 ±0.35 ±0.3 ±0.15 100 ±0.6 ±0.6 ±0.2 ±0.2 200 ±0.6 ±1.2 ±0.2 ±0.4 1,000μL 71mm 200 ±0.6 ±1.2 ±0.2 ±0.4 250 ±0.6 ±1.5 ±0.2 ±0.5 500 ±0.6 ±3 ±0.2 ±1 1,000 ±0.6 ±6 ±0.2 ±2 27
9 9.2 9.2.1 4910 Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 10μL 0.1-2.5μL 0.25 ±12.0 ±0.03 ±6.0 ±0.015 1.25 ±2.5 ±0.031 ±1.5 ±0.019 2.5 ±1.4 ±0.035 ±0.7 ±0.018 10μL 20μL 20μL, L 0.5-10μL 1 ±2.5 ±0.025 ±1.8 ±0.018 5 ±1.5 ±0.075 ±0.8 ±0.04 10 ±1.0 ±0.1 ±0.4 ±0.04 20μL 20μL, L 2-20μL 2 ±3.0 ±0.06 ±2.0 ±0.04 10 ±1.0 ±0.1 ±0.5 ±0.05 20 ±0.8 ±0.16 ±0.3 ±0.06 200μL 2-20μL 2 ±5.0 ±0.1 ±1.5 ±0.03 10 ±1.2 ±0.12 ±0.6 ±0.06 20 ±1.0 ±0.2 ±0.3 ±0.06 10-100μL 10 ±3.0 ±0.3 ±0.7 ±0.07 50 ±1.0 ±0.5 ±0.3 ±0.15 100 ±0.8 ±0.8 ±0.15 ±0.15 50-200μL 50 ±1.0 ±0.5 ±0.3 ±0.15 100 ±0.9 ±0.9 ±0.3 ±0.3 200 ±0.6 ±1.2 ±0.2 ±0.4 1,000μL 50-250μL 50 ±1.4 ±0.7 ±0.3 ±0.15 100 ±1.1 ±1.1 ±0.3 ±0.3 250 ±0.6 ±1.5 ±0.2 ±0.5 100-1,000μL 100 ±3.0 ±3 ±0.3 ±0.3 500 ±1.0 ±5 ±0.2 ±1 1,000 ±0.6 ±6 ±0.2 ±2 2,500μL 500-2,500μL 500 ±1.5 ±7.5 ±0.3 ±1.5 1,000 ±0.8 ±8 ±0.2 ±2 2,500 ±0.6 ±15 ±0.2 ±5 28
9 9.2.2 V 3111 Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 10μL 0.1-2.5μL 0.25 ±12.0 ±0.03 ±6.0 ±0.015 1.25 ±2.5 ±0.031 ±1.5 ±0.019 2.5 ±1.4 ±0.035 ±0.7 ±0.018 10μL 20μL 20μL, L 0.5-10μL 1 ±2.5 ±0.025 ±1.8 ±0.018 5 ±1.5 ±0.075 ±0.8 ±0.04 10 ±1.0 ±0.1 ±0.4 ±0.04 200μL 2-20μL 2 ±5.0 ±0.1 ±1.5 ±0.03 10 ±1.2 ±0.12 ±0.6 ±0.06 20 ±1.0 ±0.2 ±0.3 ±0.06 10-100μL 10 ±3.0 ±0.3 ±1.0 ±0.1 50 ±1.0 ±0.5 ±0.3 ±0.15 100 ±0.8 ±0.8 ±0.2 ±0.2 20-200μL 20 ±2.5 ±0.5 ±0.7 ±0.14 100 ±1.0 ±1 ±0.3 ±0.3 200 ±0.6 ±1.2 ±0.2 ±0.4 1,000μL 100-1,000μL 100 ±3.0 ±3 ±0.6 ±0.6 500 ±1.0 ±5 ±0.2 ±1 1,000 ±0.6 ±6 ±0.2 ±2 5,000μL 500-5,000μL 500 ±2.4 ±12 ±0.6 ±3 2,500 ±1.2 ±30 ±0.25 ±6.25 5,000 ±0.6 ±30 ±0.15 ±7.5 5,000μL 1-10mL 1,000 ±3.0 ±30 ±0.6 ±6 5,000 ±0.8 ±40 ±0.2 ±10 10,000 ±0.6 ±60 ±0.15 ±15 29
9 9.2.3 Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 20μL 0.5-10μL 1 ±2.5 ±0.025 ±1.8 ±0.018 5 ±1.5 ±0.075 ±0.8 ±0.04 10 ±1.0 ±0.1 ±0.4 ±0.04 200μL 5-100μL 10 ±2.0 ±0.2 ±1.0 ±0.1 50 ±1.0 ±0.5 ±0.3 ±0.15 100 ±0.8 ±0.8 ±0.2 ±0.2 300μL 20-300μL 30 ±2.5 ±0.75 ±0.7 ±0.21 150 ±1.0 ±1.5 ±0.3 ±0.45 300 ±0.6 ±1.8 ±0.2 ±0.6 1,000μL 50-1,000μL 100 ±3.0 ±3 ±0.6 ±0.6 500 ±1.0 ±5 ±0.2 ±1 1,000 ±0.6 ±6 ±0.2 ±2 5,000μL 100-5,000μL 500 ±3.0 ±15 ±0.6 ±3 2,500 ±1.2 ±30 ±0.25 ±6.25 5,000 ±0.6 ±30 ±0.15 ±7.5 30
9 9.2.4 V 3120 Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 10μL 0.1-2.5μL 0.1 ±48.0 ±0.048 ±12 ±0.012 0.25 ±12.0 ±0.03 ±6.0 ±0.015 1.25 ±2.5 ±0.031 ±1.5 ±0.019 2.5 ±1.4 ±0.035 ±0.7 ±0.018 10μL 20μL 20μL, L 0.5-10μL 0.5 ±8.0 ±0.04 ±5.0 ±0.025 1 ±2.5 ±0.025 ±1.8 ±0.018 5 ±1.5 ±0.075 ±0.8 ±0.04 10 ±1.0 ±0.1 ±0.4 ±0.04 20μL 20μL, L 2-20μL 2 ±5.0 ±0.1 ±1.5 ±0.03 10 ±1.2 ±0.12 ±0.6 ±0.06 20 ±1.0 ±0.2 ±0.3 ±0.06 200μL 2-20μL 2 ±5.0 ±0.1 ±1.5 ±0.03 10 ±1.2 ±0.12 ±0.6 ±0.06 20 ±1.0 ±0.2 ±0.3 ±0.06 10-100μL 10 ±3.0 ±0.3 ±1.0 ±0.1 50 ±1.0 ±0.5 ±0.3 ±0.15 100 ±0.8 ±0.8 ±0.2 ±0.2 20-200μL 20 ±2.5 ±0.5 ±0.7 ±0.14 100 ±1.0 ±1.0 ±0.3 ±0.3 200 ±0.6 ±1.2 ±0.2 ±0.4 300μL 30-300μL 30 ±2.5 ±0.75 ±0.7 ±0.21 150 ±1.0 ±1.5 ±0.3 ±0.45 300 ±0.6 ±1.8 ±0.2 ±0.6 1,000μL 100-1,000μL 100 ±3.0 ±3 ±0.6 ±0.6 500 ±1.0 ±5 ±0.2 ±1 1,000 ±0.6 ±6 ±0.2 ±2 31
9 Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 5,000μL 500-5,000μL 500 ±2.4 ±12 ±0.6 ±3 2,500 ±1.2 ±30 ±0.25 ±6.25 5,000 ±0.6 ±30 ±0.15 ±7.5 10mL 1-10mL 1,000 ±3.0 ±30 ±0.6 ±6 5,000 ±0.8 ±40 ±0.2 ±10 10,000 ±0.6 ±60 ±0.15 ±15 9.2.5 4830 Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 2 ±6.0 ±0.12 ±4.0 ±0.08 3 ±5.0 ±0.15 ±3.0 ±0.09 5 ±4.0 ±0.2 ±2.0 ±0.1 10 ±3.0 ±0.3 ±1.5 ±0.15 20 ±2.0 ±0.4 ±0.8 ±0.16 9.2.6 4720 Systematic error (inaccuracy) Random error (imprecision) ml % ml % ml 4720+ S 2.5 ±1.0 ±0.025 ±0.2 ±0.005 5 ±0.4 ±0.02 ±0.2 ±0.01 10 ±0.3 ±0.03 ±0.2 ±0.02 4720+ P 1 ±0.6 ±0.006 ±0.2 ±0.002 5 ±0.5 ±0.025 ±0.1 ±0.005 10 ±0.3 ±0.03 ±0.1 ±0.01 32
9 9.2.7 Xplorer Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 10μL 20μL 20μL, L 0.5-10μL 1 ±2.5 ±0.025 ±1.8 ±0.018 5 ±1.5 ±0.075 ±0.8 ±0.04 10 ±1.0 ±0.1 ±0.4 ±0.04 200μL 5-100μL 10 ±2.0 ±0.2 ±1.0 ±0.1 50 ±1.0 ±0.5 ±0.3 ±0.15 100 ±0.8 ±0.8 ±0.2 ±0.2 300μL 20-300μL 30 ±2.5 ±0.75 ±0.7 ±0.21 150 ±1.0 ±1.5 ±0.3 ±0.45 300 ±0.6 ±1.8 ±0.2 ±0.6 1,000μL 50-1,000μL 100 ±3.0 ±3 ±0.6 ±0.6 500 ±1.0 ±5 ±0.2 ±1 1,000 ±0.6 ±6 ±0.2 ±2 5,000μL 250-5,000μL 500 ±3.0 ±15 ±0.6 ±3 2,500 ±1.2 ±30 ±0.25 ±6.25 5,000 ±0.6 ±30 ±0.15 ±7.5 10mL 0.5-10mL 500 ±3.0 ±30 ±0.6 ±6 2,500 ±0.8 ±40 ±0.2 ±10 5,000 ±0.6 ±60 ±0.15 ±15 33
9 9.3 9.3.1 M 3114 μl μl Systematic error (inaccuracy) Random error (imprecision) % μl % μl 10μL 20μL 20μL, L 0.5-10μL 8 1 ±8.0 ±0.08 ±5.0 ±0.05 5 ±4.0 ±0.2 ±2.0 ±0.1 10 ±2.0 ±0.2 ±1.0 ±0.1 12 8 200μL 10-100μL 8 10 ±3.0 ±0.3 ±2.0 ±0.2 50 ±1.0 ±0.5 ±0.8 ±0.4 100 ±0.8 ±0.8 ±0.3 ±0.3 12 8 300μL 30-300μL 8 30 ±3.0 ±0.9 ±1.0 ±0.3 150 ±1.0 ±1.5 ±0.5 ±0.75 300 ±0.6 ±1.8 ±0.3 ±0.9 12 8 34
9 9.3.2 4860 Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 10μL 20μL 20μL, L 0.5-10μL 1 ±5.0 ±0.05 ±3.0 ±0.03 5 ±3.0 ±0.15 ±1.5 ±0.075 10 ±2.0 ±0.2 ±0.8 ±0.08 200μL 5-100μL 10 ±2.0 ±0.2 ±2.0 ±0.2 50 ±1.0 ±0.5 ±0.8 ±0.4 100 ±0.8 ±0.8 ±0.25 ±0.25 300μL 20-300μL 30 ±2.5 ±0.75 ±1.0 ±0.3 150 ±1.0 ±1.5 ±0.5 ±0.75 300 ±0.6 ±1.8 ±0.25 ±0.75 1,250μL 50-1,200μL 120 ±6.0 ±7.2 ±0.9 ±1.08 600 ±2.7 ±16.2 ±0.4 ±2.4 1,200 ±1.2 ±14.4 ±0.3 ±3.6 9.3.3 M 3122 Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 10μL 20μL 20μL, L 0.5-10μL 0.5 ±12.0 ±0.06 ±8.0 ±0.04 1 ±8.0 ±0.08 ±5.0 ±0.05 5 ±4.0 ±0.2 ±2.0 ±0.1 10 ±2.0 ±0.2 ±1.0 ±0.1 200μL 10-100μL 10 ±3.0 ±0.3 ±2.0 ±0.2 50 ±1.0 ±0.5 ±0.8 ±0.4 100 ±0.8 ±0.8 ±0.3 ±0.3 300μL 30-300μL 30 ±3.0 ±0.9 ±1.0 ±0.3 150 ±1.0 ±1.5 ±0.5 ±0.75 300 ±0.6 ±1.8 ±0.3 ±0.9 35
9 9.3.4 Xplorer Systematic error (inaccuracy) Random error (imprecision) μl % μl % μl 10μL 20μL 20μL, L 0.5-10μL 1 ±5.0 ±0.05 ±3.0 ±0.03 5 ±3.0 ±0.15 ±1.5 ±0.075 10 ±2.0 ±0.2 ±0.8 ±0.08 200μL 5-100μL 10 ±2.0 ±0.2 ±2.0 ±0.2 50 ±1.0 ±0.5 ±0.8 ±0.4 100 ±0.8 ±0.8 ±0.25 ±0.25 300μL 15-300μL 30 ±2.5 ±0.75 ±1.0 ±0.3 150 ±1.0 ±1.5 ±0.5 ±0.75 300 ±0.6 ±1.8 ±0.25 ±0.75 1,250μL 50-1,200μL 120 ±6.0 ±7.2 ±0.9 ±1.08 600 ±2.7 ±16.2 ±0.4 ±2.4 1,200 ±1.2 ±14.4 ±0.3 ±3.6 36
9 9.4 20~25 C ±0.5 C 10 ISO 8655 stream/xstream DIS speed level 7 9.4.1 4981 ml μl Systematic error (inaccuracy) Random error (imprecision) % μl % μl 0.1 2 ±1.6 ±0.032 ±3.0 ±0.06 20 ±1.0 ±0.2 ±2.0 ±0.4 0.2 4 ±1.3 ±0.052 ±2.0 ±0.08 40 ±0.8 ±0.32 ±1.5 ±0.6 0.5 10 ±0.9 ±0.09 ±1.5 ±0.15 100 ±0.8 ±0.8 ±0.6 ±0.6 1 20 ±0.9 ±0.18 ±0.9 ±0.18 200 ±0.6 ±1.2 ±0.4 ±0.8 2.5 50 ±0.8 ±0.4 ±0.8 ±0.4 500 ±0.5 ±2.5 ±0.3 ±1.5 5 100 ±0.6 ±0.6 ±0.6 ±0.6 1,000 ±0.5 ±5 ±0.25 ±2.5 10 200 ±0.5 ±1 ±0.6 ±1.2 2,000 ±0.5 ±10 ±0.25 ±5 25 500 ±0.4 ±2 ±0.6 ±3 5,000 ±0.3 ±15 ±0.25 ±12.5 50 1,000 ±0.3 ±3 ±0.5 ±5 10,000 ±0.3 ±30 ±0.3 ±30 37
9 9.4.2 stream/xstream ml μl Systematic error (inaccuracy) Random error (imprecision) % μl % μl 0.1 10 ±1.6 ±0.16 ±2.5 ±0.25 50 ±1.0 ±0.5 ±1.5 ±0.75 100 ±1.0 ±1 ±0.5 ±0.5 0.2 20 ±1.3 ±0.26 ±1.5 ±0.3 100 ±1.0 ±1 ±1.0 ±1 200 ±1.0 ±2 ±0.5 ±1 0.5 50 ±0.9 ±0.45 ±0.8 ±0.4 250 ±0.9 ±2.25 ±0.5 ±1.25 500 ±0.9 ±4.5 ±0.3 ±1.5 1 100 ±0.9 ±0.9 ±0.55 ±0.55 500 ±0.6 ±3 ±0.3 ±1.5 1000 ±0.6 ±6 ±0.2 ±2 2.5 250 ±0.8 ±2 ±0.45 ±1.125 1,250 ±0.5 ±6.25 ±0.3 ±3.75 2,500 ±0.5 ±12.5 ±0.15 ±3.75 5 500 ±0.8 ±4 ±0.35 ±1.75 2,500 ±0.5 ±12.5 ±0.25 ±6.25 5,000 ±0.5 ±25 ±0.15 ±7.5 10 1,000 ±0.5 ±5 ±0.25 ±3 5,000 ±0.4 ±20 ±0.25 ±13 10,000 ±0.4 ±40 ±0.15 ±15 25 2,500 ±0.3 ±8 ±0.35 ±8.8 12,500 ±0.3 ±38 ±0.25 ±31 25,000 ±0.3 ±75 ±0.15 ±38 50 5,000 ±0.3 ±15 ±0.50 ±25 25,000 ±0.3 ±75 ±0.20 ±50 50,000 ±0.3 ±150 ±0.15 ±75 38
9 9.5 20~25 C ±0.5 C 10 ISO 8655 9.5.1 Systematic error (inaccuracy) Random error (imprecision) 0.5-2.5mL 2.5mL ±0.6% 0.1% 1.0-5.0mL 5.0mL ±0.5% 0.1% 2-10mL 10mL ±0.5% 0.1% 5-25mL 25mL ±0.5% 0.1% 10-50mL 50mL ±0.5% 0.1% 20-100mL 100mL ±0.5% 0.1% 9.5.2 Systematic error (inaccuracy) Random error (imprecision) M 25mL ±0.2% 0.1% H 50mL ±0.2% 0.1% 39
9 9.6 EN ISO 8655 μl 9.6.1 Error limit for systematic error (inaccuracy) 1μL 2μL 5μL 10μL 20μL 50μL 100μL 200μL 500μL 1,000μL 2,000μL 5,000μL 10,000μL ±5.0% ±4.0% ±2.5% ±1.2% ±1.0% ±1.0% ±0.8% ±0.8% ±0.8% ±0.8% ±0.8% ±0.8% ±0.6% ±0.05μL ±0.08μL ±0.125μL ±0.12μL ±0.2μL ±0.5μL ±0.8μL ±1.6μL ±4.0μL ±8.0μL ±16.0μL ±40.0μL ±60.0μL Error limit for random errors (imprecision) ±5.0% ±0.05μL ±2.0% ±0.04μL ±1.5% ±0.075μL ±0.8% ±0.08μL ±0.5% ±0.1μL ±0.4% ±0.2μL ±0.3% ±0.3μL ±0.3% ±0.6μL ±0.3% ±1.5μL ±0.3% ±3.0μL ±0.3% ±6.0μL ±0.3% ±15.0μL ±0.3% ±30.0μL 2 40
9 9.6.2 4830 Error limit for systematic error (inaccuracy) Error limit for random errors (imprecision) 5μL ±2.5% ±0.13μL ±1.5% ±0.08μL 10μL ±2.0% ±0.2μL ±1.0% ±0.1μL 20μL ±2.0% ±0.4μL ±0.8% ±0.16μL 50μL ±1.4% ±0.7μL ±0.6% ±0.3μL 100μL ±1.5% ±1.5μL ±0.6% ±0.6μL 200μL ±1.5% ±3.0μL ±0.4% ±0.8μL 500μL ±1.2% ±6.0μL ±0.4% ±2.0μL 1,000μL ±1.2% ±12.0μL ±0.4% ±4.0μL 9.6.3 Error limit for systematic error (inaccuracy) Error limit for random errors (imprecision) 0.001mL ±5.0% ±0.05μL ±5.0% ±0.05μL 0.002mL ±5.0% ±0.1μL ±5.0% ±0.1μL 0.003mL ±2.5% ±0.075μL ±3.5% ±0.11μL 0.01mL ±2.0% ±0.2μL ±2.5% ±0.25μL 0.02mL ±1.5% ±0.3μL ±2.0% ±0.4μL 0.05mL ±1.0% ±0.5μL ±1.5% ±0.75μL 0.1mL ±1.0% ±1.0μL ±1.0% ±1.0μL 0.2mL ±1.0% ±2.0μL ±1.0% ±2.0μL 0.5mL ±1.0% ±5.0μL ±0.6% ±3.0μL 1mL ±1.0% ±10.0μL ±0.4% ±4.0μL 2mL ±0.8% ±16.0μL ±0.4% ±8.0μL 5mL ±0.6% ±30.0μL ±0.3% ±15.0μL 10mL ±0.5% ±50.0μL ±0.3% ±30.0μL 25mL ±0.5% ±125.0μL ±0.3% ±75.0μL 50mL ±0.5% ±250μL ±0.25% ±125.0μL 100mL ±0.5% ±500μL ±0.25% ±250.0μL 200mL ±0.5% ±1,000μL ±0.25% ±500.0μL 41
9 9.6.4 Error limit for systematic error (inaccuracy) Error limit for random errors (imprecision) 0.01mL ±2.0% ±0.2μL ±1.0% ±0.1μL 0.02mL ±2.0% ±0.4μL ±0.5% ±0.1μL 0.05mL ±1.5% ±0.75μL ±0.4% ±0.2μL 0.1mL ±1.5% ±1.5μL ±0.3% ±0.3μL 0.2mL ±1.0% ±2.0μL ±0.3% ±0.6μL 0.5mL ±1.0% ±5.0μL ±0.2% ±1.0μL 1mL ±0.6% ±6.0μL ±0.2% ±2.0μL 2mL ±0.6% ±12.0μL ±0.2% ±4.0μL 5mL ±0.6% ±30.0μL ±0.2% ±10.0μL 10mL ±0.6% ±60.0μL ±0.2% ±20.0μL 25mL ±0.6% ±150.0μL ±0.2% ±50.0μL 50mL ±0.6% ±300.0μL ±0.2% ±100.0μL 100mL ±0.6% ±600.0μL ±0.2% ±200.0μL 200mL ±0.6% ±1,200μL ±0.2% ±400.0μL 9.6.5 Error limit for systematic error (inaccuracy) Error limit for random errors (imprecision) 1mL ±0.6% ±6.0μL ±0.1% ±1.0μL 2mL ±0.5% ±10.0μL ±0.1% ±2.0μL 5mL ±0.3% ±15.0μL ±0.1% ±5.0μL 10mL ±0.3% ±30.0μL ±0.1% ±10.0μL 10mL ±0.2% ±40.0μL ±0.1% ±20.0μL 25mL ±0.2% ±50.0μL ±0.1% ±25.0μL 50mL ±0.2% ±100.0μL ±0.1% ±50.0μL 100mL ±0.2% ±200μL ±0.1% ±100.0μL 42
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