Division of Biofunctional Chemistry The Chemical Society of Japan Vol. 19, No.3 (2004.12. 1) 1 3 4 8-12 16 19 20 5 21 19 22 24 8 26 7 27
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Chem. & Eng News (Oct. 25, 2004) Insight Nobel Prizes and Fuzzy Boundaries Experience shows you don t have to be a chemist to win the Nobel Prize in Chemistry 2003 2004 Wennerstr Lund Univ. 1962 Perutz Kendrew Watson, Crick Wilkins - 2 -
Top Scientist 63 ERATO ICORP SORST COE 21COE 53 60 Izatt-Christensen 1998 Backer Lecture 1999 Vielberth Lectureship 2002 2004 2003 786 138 ISI-Thomson Scientific 100 1990 2000 10 1993 2003 8-3 -
ON-OFF ON-OFF 1), 3) 1. N N Zn N N N N N Zn Zn N N N Zn N N (NO 3 ) 4 (NO 3 ) 4 1 2 400 1 2 1 0 1 2 1, 2 1 400 450 500 550 1 Fluorescence titration curve of 1 with the phosphorylated - 4 - Fluorescence Intensity (a.u.) 300 200 100 0 F/Fo-1 0 Wavelength (nm) peptide (µm) Fluorescence Spectrum change of 1 (1 µm) upon the addition of a phosphorylated peptide (EEEIpYEEFD) (Inset) peptide (l ) and the corresponding non-phosphorylated peptide (s ).
M -1 (Figure 1) 10 3 M -1 Dpa-Zn(II) 1 X 2 1, 2 PET 3 10 7 Zn P Zn 2. 1, 2 3, 4 2) N Zn N N N N N N Zn Zn N N N N 3 N N 4 N N Zn N - 5 -
CD 3, 4 3 i, i+7 (K app = 1.6 x 10 6 M -1 ) α- α- 3, 4 3. 3) i) ii) 1, 2 PTP1B PTP1B 4) SDS-PAGE (SDS-PAGE) 2 iii) WW 4-6 -
WW 1) Akio Ojida, Yasuko Mito-oka, Masa-aki Inoue, Itaru Hamachi J. Am. Chem. Soc. 2002, 124, 6256. 2) Akio Ojida, Masa-aki Inoue, Yasuko Mito-oka, Itaru Hamachi J. Am. Chem. Soc. 2003, 125, 10184. 3) Akio Ojida, Yasuko Mito-oka, Kazuki Sada, Itaru Hamachi J. Am. Chem. Soc. 2004, 126, 2454. 4) Akio Ojida, Takahiro Kohira, Itaru Hamachi Chem. Lett. 2004, 33, 1024. - 7 -
e-mail oga@ms.naist.jp S 400 nm 2λ (800 nm λ 800 nm 400 nm (400 nm) 2λ (800 nm (PDT) PDT PDT PDT S 0 1. p 1) 2) 2 180 (3) ( ) ( ) (4D) 3) (5D) - 8 -
2. 6 (7P) GPC (8) 8 (5D) GPC 3. 5D :4D :W avelength /nm s (2) Z-scan 2 400 8000 300 4D 4D s (2) 6000 200 964 nm 370 GM 4000 0 5D s (2) 2000 887nm 7600 GM 4D 20 0 800 900 1000 1100 1200 1300 σ ( 2) / G M 100 900 1100 1300-9 -
(5M) (7M) (8) 5M 7M 5D 7P 3000 1-5M s (2) 5D 1/4 1800 GM 5M 1800 GM 7M 1200 GM 8 1000 GM 2 5) sample 4D 5D 5M 7M 8 σ (2) / GM 370 7600 1800 1200 1000 1000 GM GM PDT 1.5 2 GM 5D 7600 GM 4000 PDT 4. PDT QOL 630 nm PDT 5D PDT 733 nm 1270 nm 5D PDT (9) 9-10 -
5D HeLa PDT PDT PDT PDT 1) (a) Albota, M. et al. Science 1998, 281, 1653; (b) Reinhardt, B. A. et al. Chem. Mater. 1998, 10, 1863 2) (a) Ogawa, K.; Kobuke, Y. Angew. Chem., Int. Ed. 2000, 39, 4070; (b) Kobuke, Y.; Ogawa, K. Bull. Chem. Soc. Jpn. 2003, 78, 689. 3) Ogawa, K.; Zhang, T.; Yoshihara, K.; Kobuke, Y. J. Am. Chem. Soc. 2002, 124, 22. 4) Ogawa, K.; Ohashi, A.; Kobuke, Y.; Kamada, K.; Ohta, K. J. Am. Chem. Soc. 2003, 125, 13356. 5) Zhou, X; Ren, A.-M.; Feng, J.-K. Chem. Eur. J. 2004, 10, 5623. - 11 -
- nojimtcm@mbox.nc.kyushu-u.ac.jp http://www.takenaka.cstm.kyushu-u.ac.jp/ DNA (1) DNA(ssDNA) (2) ssdna - (POC) (3) (APEG) - 1 (A) 5 - ssdna (B) DNA POC (C) APEG DNA DNA (D) - 1. - (POC) (A) DNA(ssDNA) (B) POC (C) APEG (D) - 12 -
1. POC A(RNase A) 2 RNase A 20 S-peptide 104 (S-protein) i RNase S-peptide POC S- peptide-s-protein POC APEG 2 POC ii 19 mer S-peptide FastMoc iii N- 5-24 5 -HS-(CH 2 ) 6 -CGA CGT TGT AAA ACG ACG GCC AGT-3 HPLC HPLC MALDI TOF-MS APEG PEG(M r =4000) 90 100 mm NaCl DNA 3.3 x 10 4 M -1 iv n=9 2. (A) - (POC) (B) APEG. POC (QCM) v 3 QCM (2.5 mmφ, 4.9 mm 2 ) - 5 -SH ssdna(poc ) ssdna POC ( 3A ) QCM (Affinix Q vi, - 13 -
Initium, Inc.) APEG ( 3A ) APEG POC 3B DNA QCM 3. QCM APEG (A) ssdna POC ( ) APEG ( ) APEG (B) DNA ( ) APEG ( ) ssdna APEG ( ) 2. POC - PEG S-peptide S-peptide PEG S-protein S-peptide-S-protein QCM 2 (BSA)(pI=4.7) A(RNase A)(pI=9.3) APEG BSA POC A B 4. - APEG (A) APEG BSA ( ) S-protein ( ) (B) APEG BSA ( ) S-protein ( ) - 14 -
QCM ( 4A) BSA S-protein BSA S- peptide-s-protein POC QCM APEG BSA S-protein ( 4B) APEG POC BSA RNase A 4B (data not shown) APEG DNA vii i) R. T. Raines, Chem. Rev., 98, 1045-1065 (1998). ii) N. J. Ede, G. W. Tregear and J. Haralambidis, Bioconjugate Chem., 5, 373 (1994). iii) K. Ohtsuka, R. Kajiki, M. Waki, T. Nojima and S. Takenaka, Analyst, 129, 888-889 (2004). iv) K. Ohtsuka, K. Uemura, T. Nojima, M. Waki and S. Takenaka, in preparation. v) S. Fukusho, H. Furusawa and Y. Okahata, Chem. Commun., 2002, 88-89 (2002). vi) http://www.initium2000.com/affinix_q.html vii) - 15 -
1 1. RNA N RNA N RNA RNA 1A, B N RNA N RNA 1C RNA - 16 -
N RNA RNA 1B RNA RNA (A) N RNA (B) N RNA (C) 5 2, 3 2. - - DNA ATP DNA ATP-dependent DNase 2-17 -
DNA ATP DNase DNA 1 : 1 2a ATP DNA k cat - - (a) ATP (b) ATP 4 3. - 3 E1 ATP E2 E3 E1 E1 3A E1 PPi E1 E1 3B - 18 -
3 (A) ATP E1 (B) E1 1, 5, 30 (1) H. Furusawa, A. Murakawa, S. Fukusho, and Y. Okahata, ChemBioChem, 217-220 (2003) (2) H. Matsuno, H. Furusawa, and Y. Okahata, Chem. Commun., 470-471 (2002) (3) H. Matsuno, H. Furusawa, and Y. Okahata, Biochemistry, in press (2004) (4) H. Furusawa, A. Minamide, and Y. Okahata, submitted - 19 -
19 6 10 11 10 8 9 -DNA 300 - - 200 20-20 -
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14 10 29 DNA 3 SNP 45 NO PeT photoinduced electron transfer 8 diabody DNA - 24 -
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7 2005 2005 1 21 1-2-45 12 24 1. Back To The Cell 2. 3. 4. 5. 6. 7. ( ) A4 2.5 cm Microsoft word 4,000 5,000 5,000 6,000 6,000 7,000 2,000 3,000-27 -
02250 5 41364 2005 FAX 981-8555 1-1 022-717-8808 FAX 022-717-8807 ogawa@biochem.tohoku.ac.jp http://www-pclab.ph.tokushima-u.ac.jp/fbc/7thsymp.html - 28 -
Vol.19, No.3 2004 12 1 101-8307 1-5, Office of the Secretary ; The Chemical Society of Japan, 1-5 Kanda-Surugadai,Chiyodaku,Tokyo 101-8307,Japan URL:http://seitai.chemistry.or.jp/ mailto:seitai@chemistry.or.jp - 29 -