A) " " B a. b. c. i. ii. iii. Bartholomew & Mittwer a. b. a. (Ziehl-Neelsen) b. Truan't Auramine-Rhodamine stain c. "Tbcolor Merck"
2. A) 1) 2) (v/v) 3g 30ml 60ml (2% 5ml, 5 ) 3) " "
AFB AFB 20ml 20ml 10 AFB ( ) Thimerosol 0.02% 0.50ml 80ml 20ml ( ) B) a. 1884 Hans Christian Gram Hucker
b. 100 1983 Davis Davis, J. A.,et al. J.Bacteriol.,156,837-854,1983. c. i. () 10 100ml 1% 1g 2g
300ml v/v 2.5 100ml 510 11g 100ml 10ml 5 5ml 510 30 10~20
, ii. Hucker 1020% 0.2g 20ml 0.8g 80ml 2g 100ml 20mg 100
iii. Bartholomew & Mittwer Bartholomew & Mittwer (B&M)Hucker 5 2 (Hucker ) 1 5 30 2 30 30 Hiss halo
a. 1 0.3% Indian ink( 1%) b. 1 20% 1 2 31 420% OLT
Lembach-Sous 0.9 0.3 95100 95 1.5 3.0 10 10
a.(ziehl-neelsen) 3 35 1015 30
b. Truan't Auramine-Rhodamine stain 1.50g 0.75 10ml 50ml 75ml 25ml 70 0.5 3 0.5 10 1520 23 30 B 500nm 530nm
c. "Tbcolor Merck" 3 II 0.8% I I II 10 II 10 2% 3%
impression film Dienes Dienes 2.5g 1.25g 2.5g 0.25g 100ml 10g Dienes 10g 10g 20g 0.05g 100ml
,18:43-49.1991. Davis, J. A.,et al. : Chemical mechanism of the gram stain and synthesis of new electron opaque marker for electron microscopy which replaces the iodine mordant of the stain.j.bacteriol.,156,837-854,1983. 3..,31,944-948,1982 MERCK,,1993,