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- あいり ひがき
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1
2 2016/17 P 1 P 8 P13 P18 P23 P28 7 P33 P41 15 P48 P65 GC-MS/MSLC-MS/MS
3 2016/ AH3 75.4% HI AH1pdm09 B AH3 D151GD151N HA AH1pdm09 AH3 B /17-1 -
4 well MDCKLLC-MK2HEp-2HeLa RD-18sVero Caco /17 1) 0.75% HI A(H1N1)pdm09 B 3 A(H3N2) 2 QIAamp Viral RNA Mini Kit QIAGEN RNA OneStep RT-PCR Kit QIAGEN NA RT-PCR PCR QIAQuick PCR Purification Kit QIAGEN DNA BigDye Terminator v3.1 Cycle Sequencing Kit Applied BiosystemsBigDye XTerminator KitApplied BiosystemsApplied Biosystems 3500 MEGA6 NJ AH1pdm09 NA H275Y 1) AH3 NA E119VD151VQ226HG248RK249ER292KN294S 1,3,4) B NA R150KD197ED197NI221TN294SG407S 1) 2016/ ) 6) - 2 -
5 / AH1pdm09 1.5%AH %B 7.7% B % B Victoria 15.4% A H1pdm09 1.5% B Yamagata 7.7% A H3 75.4% - 3 -
6 2016/ B Victria B Yamagata AH3 AH1pdm /17 AH3 2 AH1pdm B B 1:2 1:1.6 7)B AH3 D151GD151N NA HI AH3 49 A/Hong Kong/4801/2014 NA D151GD151N HI HA 3C.2a 10% 7) - 4 -
7 3C.2a 3C.2 3C.3a 3C.3 AH3 HA AH1pdm09 B 10 HI AH1pdm /18 WHO A/Michigan/45/2015 8) A/California/7/2009 AH3 2014/ /15 A/Hong Kong/4801/2014 A/Switzerland/ B - 5 -
8 A(H1N1)pdm09 NA A(H3N2) NA B NA /16-6 -
9 70% 1) :, 3, ),,,:,60,61-66,2009 3) Monto,A.S.,McKimm-Berschkin,et al.:antimicrobial Agents and Chemotherapy.,50, ,2006 4) Sheu,T.G.,Deyde,et al.:antimicrobial Agents and Chmotherapy.,52, , ) : l ) ; nsenshou01/houdou.html ),;2016/ ) WHO;Recommended composition of influenza virus vaccines for use in the northern hemisphere influenza season tion.pdf?ua=
10 9 28.6% 5~ g MPN MPN AD g No.2OXOID OXOIDOXOID - 8 -
11 9ml OXOID CCDA OXOID OXOID C.jejuniC.coli 10g C.jejuniC.coli McFarland 1 1ml 150g High Dose McFarland ml 150g (Low Dose) 25g g 100ml mL 9mL 3 100g MPN AD % % 7 C9 CD10 C1 C 2 C A B 6.9%5 /72-9 -
12 100.0% 80.0% 60.0% 40.0% 20.0% 0.0% 14.3% 28.6% 14.3% 14.3% 0.0% 0.0% 0.0%0.0% 0.0% 100.0% 80.0% 60.0% 40.0% 20.0% 0.0% 11.1% 0.0% 0.0% 0.0% 0.0% 33.3% 11.1% 0.0% A-1 A-2 B-1 B-2 C-1 C-2 D-1 D High dose Low Dose High Dose 56 Low Dose 28 MPN
13 ~7 6.9%5 / H %8 5 H %5 1 H %8/28 H %(17/168 ) H
14 - 12 -
15 - 13 -
16 Copy G
17 Copy G
18 - 16 -
19 - 17 -
20 LC/MS/MS (1) LC/MS 1mg/ml (2) 2 1 (3) (4) Waters Xevo TQD 1-1 LC Waters:HSS C181.8µm mm µL A B ml/min
21 Time(min) A B MS ESI+ 1.5kV 1200 L/hr MRM m/z CV(V) CE(eV) C (1) 1.0 g 100mL 5mL SepPak AC-2 400mg 3 C18(1g)tC18(900mg)HLB(500mg Waters C18 82% 2 60% 40% 1 15mL 5mL 15mL30mL45mL 2 C18 5mL BL C18 C18 HLB 82 BL mL 30mL 45mL
22 10 5mL 10mL 3 C18 15mL BL mL 10mL 5mL 10mL 40 (2) 4 13 C ppb ppb (3) ng/g 1000 ng/g
23 ng/g (ng/g) % % % ppb 2 ~ ~ 50 ng/g % 3 % % % 1000 % % LC/MS/MS
24
25 (1) GC 257 LC (2) %8.4(4.2) 10%(27.6) Trace0.83 % (3) -2 (4) 3 0.1mg/kg (5) GC 7890B MS 7000CAgilent LC ACQUITY UPLC-H Class MS Xevo TQD Waters
26 1-1 GC/MS/MS VF5MS 0.25mm 30m 0.25µm 70(2 ) 25/ 150 3/ 200 8/ 300(4 ) µl EI 70eV 1-2 LC/MS/MS Waters:HSS C18 1.8µm mm 45 A5mmol/L B5mmol/L 0.4 ml/min 2 µl A B L/hr550 ESI 0.75V (1) -2 A B 10 ml PSA ODSSep-Pak Vac 1g 2g Waters GC/NH2InertSep GC/NH2(500mg/500mg/6mL GL ) 2 GC/MS/MS LC/MS/MS GC/MS/MS PEG300 50ppm GC PEG ppm (2) GC
27 50200 RSD% GC LC % A % A A B
28 ODS 50 S/N 10 A B
29 - 27 -
30 (1) (2) (3) HLB200 mg 6 ccprime-hlb 200 mg 6 cc Waters (4) 5.0g 3 0.5ppm ppm (5) 100ppm 5ppm 2:8 (6) Waters Xevo TQD LC ACQUITY UPLC-H Class 1-1 LC Waters:BEH C18 1.7µm mm 50 A0.05 B 0.4 ml/min 10 µl Posi Time(min) A B MS ESI(+) 0.75 kv (+) 1200L/hr
31 33L/hr MRM (m/z) CVV CE(eV) RT(min) 1 Oxytetracycline Chlortetracycline Tetracycline Oxolinic_acid Ormetoprim Sulfaquinoxaline Sulfadimidine Sulfamonomethoxine Sulfamerazine Sulfamonomethoxine Trimethoprim Pyrimethamine Flubendazole RT 10mL 5.0 HLB PRiME HLB 0.2%(1:1:3) 50mL 10 ml 3,000rpm 10min 10 ml ml 30mL20mL ml 1.6 ml ml ( 5 5 ml (9:15 ml 10 ml Filter 0.2 µm LC/MS/MS
32 (1) (2) HLB HLB N PRiME-HLB HLB 70120RSD10 HLB PRiME-HLB N HLB PRiME -HLB (3) LC
33 2 HLB (ppm) (ppm) 0.5g 10mL RSD RSD Oxytetracycline Chlortetracycline Tetracycline Oxolinic_acid Ormetoprim Sulfaquinoxaline Sulfadimidine Sulfamonomethoxine Sulfamerazine Sulfadimethoxine Trimethoprim Pyrimethamine Flubendazole PRiME-HLB 0.5g 10mL g 5mL 10 (ppm) (ppm) RSD RSD Oxytetracycline Chlortetracycline Tetracycline Oxolinic_acid Ormetoprim Sulfaquinoxaline Sulfadimidine Sulfamonomethoxine Sulfamerazine Sulfadimethoxine
34 Trimethoprim Pyrimethamine Flubendazole HLB PRiME-HLB
35 - 33 -
36
37 100cm 2,12.5cm8cm100ml 50ml 200rpm 10min 0.2m ICS-1100 g/cm 2 / (g/cm 2 /day)
38 mg/m 2 NaCl E=1/2 mv 2 m: v:e
39 (m/s)2 (g/cm2/day) ^ /2 (mg/m 2 )NaCl
40 1/2 482m 181 9cm cm
41 () mg/m 2 NaCl mg/m 2 NaCl
42 mg/m 2 (m/s) 2 NaCl
43 m 4m 1.2km 1.2km 1.3km 1km m 15m 1.7km
44 US cm ) 1 1 ph EC SO4 2 NO3 Cl NH4 Na K Ca 2 Mg 2 ph EC ph EC 1 28 ph ph 5.6 EC 3.91mS/m 3.66mS/m phec ph EC [ms/m] H19 H20 H21 H22 H23 H24 H25 H26 H27 H H19 H20 H21 H22 H23 H24 H25 H26 H27 H28 1 ph EC
45 Na Cl nss-so4 2 nssnon-sea salt NO3 [%] Na+ Cl- Mg2+ ss-so42- K+ nss-so42- ss-ca2+ NO3- nss-ca2+ NH4+ H28 5 H28 5 H+ 2 R1R2 28 R1 R2 3 R10 86% R1 R2 R1 [%] Cations + Anions [μeq/l] R2 [%] EC [ms/m] 3 R1 R2 EC EC Na K
46 NO3 nss-so4 2 NH4 9 3) 6 ph EC [mm] [ms/m] Na + K + [μmol/l] [μmol/l] NO 3 - nss-so 4 2- [μmol/l] [μmol/l] NH 4 + ph [μmol/l] ph unit H28 H28 H23H27 H23H27 H23H
![4 2 5 5 5 24 28 6 7 EC Na + [mm] 400 300 200 100 y = 0.8778x + 15.288 R² = 0.](/docs-images/98/137890006/images/47-0.jpg "84 [ms/m] 12 10 8 6 4 2 y = 1.0224x - 0.048 R² = 0.")
![9485 [μmol/l] 700 600 500 400 300 200 100 y = 1.0043x - 3.8525 R² = 0.](/docs-images/98/137890006/images/47-1.jpg "9517 0 0 100 200 300 400 0 0 2 4 6 8 10 12 0 0 100 200 300 400 500 600 700 [mm]")
47 EC Na + [mm] y = x R² = 0.84 [ms/m] y = x R² = [μmol/l] y = x R² = [mm] [ms/m] [μmol/l] 6 ECNa 1 1 EC Na 11km
48 EC Na Na Cl EC EC nss-so 4 2- NO 3 - NH y = x R² = y = x R² = y = x R² = [μmol/l] [μmol/l] 40 [μmol/l] [μmol/l] [μmol/l] [μmol/l] ph nss-ca y = 0.701x R² = [μmol/l] y = x R² = [μmol/l] 7 nss-so4 2 NO3 NH4 phnss-ca nss-so4 2 NO3 NH4 ph nss-ca 2 4m 15m ph
49 ph EC Na + Cl EC Na K NO3 nss-so4 2 NH4 9 2 nss-so4 2 NO3 NH4 ph nss-ca ) https:// 2) EANETTechnical Manual for Wet Deposition Monitoring in East Asia )
50 (1) ,24 (2) 1 St- 1 St- 2 St- 3 St- 4 St- 1 St- 2 St- 3 St-3 St-2 St-1 St-1 St-2 St-3 St
51 (3) 20 ph (4) 2 Shannon ASPT ASPT 10 ASPT ASPT ASPT ASPT ASPT =() 29 4 Shannon Shannon Weaver1963 () H n N ln n N S N nii 3 (1) 1 2 (a)(b) 3 4 Shannon -1 St- 1 30m 20cm
52 ASPT Shannon St- 2 30m 20cm 9 7 ASPT Shannon St- 3 40m 30cm ASPT ASPT Shannon St- 4 20m 40cm ASPT ASPT 26 Shannon
53 2 (a) (b)
54 3 4 4 ph 7 SS St DO St- 1St- 2St- 3 St ASPT St- 2 St- 1 St- 3St- 4 Shannon 26 St- 1 St- 2 St- 3St- 4 BOD
55 2 BOD75 ASPT BOD75% 10 ASPT St St- 1St- 3St BOD75%(mg/l) BOD75% ASPT H18 H19 H20 H21 H22 H23 H24 H25 H26 H27 H ASPT BOD75%(mg/l) BOD75% ASPT H18 H19 H20 H21 H22 H23 H24 H25 H26 H27 H ASPT (a) St- 1 (c)st- 3 BOD75%(mg/l) BOD75% ASPT H18 H19 H20 H21 H22 H23 H24 H25 H26 H27 H ASPT BOD75%(mg/l) BOD75% ASPT H18 H19 H20 H21 H22 H23 H24 H25 H26 H27 H ASPT (b) St- 2 (d)st- 4 2 BOD75% ASPT
56 (2) 5 6 (a)(b) 7 ASPT 8 Shannon 5-1 St- 1 3m 40 29cm/s 34m ASPT ASPT Shannon St- 2 6m 60 18cm/s ASPT 6.0 ASPT Shannon
57 6 (a) (b) -3 St- 3 8m 70cm ASPT ASPT Shannon
58 7 ASPT 8 8 ph St- 1 St- 3DO St- 1 St ASPT ASPT St- 2 7 ASPT ASPT St- 3 1 ASPT ASPT ASPT ASPT St
59 1 St- 3 Shannon St- 2 8 Shannon ASPT ASPT 3 St- 1 St- 3 BOD75 St- 1St BOD75 ASPT St St- 2St- 3 ASPT ASPT 4 (1) ASPT St- 2 St- 1 St- 3St- 4 Shannon 28 St- 1St- 3 St St BOD75%
60 ASPT St St- 1St- 3St BOD75%(mg/l) BOD75% ASPT ASPT H19 H20 H21 H22 H23 H24 H25 H26 H27 H ASPT (a)st- 1 1 (c)st- 3 2 BOD75%(mg/l) BOD75% ASPT ASPT H19 H20 H21 H22 H23 H24 H25 H26 H27 H ASPT BOD75%(mg/l) ASPT ASPT H19 H20 H21 H22 H23 H24 H25 H26 H27 H ASPT (b)st- 2 3 BOD75% ASPT (2) ASPT ASPT St- 1 St- 3 Shannon 3 St- 2 St- 1St BOD75 ASPT St St- 2St- 3 ASPT ASPT (3)
61
62 St
63 St- 2 St
64 St
65 (2) St
66 St- 2 St
67 12 COD 28 COD 2 COD COD COD COD COD COD No.2 No No.3 No1,3,4,6,7 No10,11,16 No1,2,3 ( 1) No11 No No7 B COD 3.0mg/L 8 A 2.0mg/L No.1 No.1 No.4 No.3 No.6 No.7 No.10 No
68 2.1 COD COD (75) 1 COD 1 COD75% (mg/l) No1,3,4,6,7 5 No6 No10 No1,2,3 28 No6 No ,8, No6,7,10 No m 3m 1m COD 4. 66
69 COD COD No No7 6 8 No10 No6 6 8 No No mg/L No6,7 6 6,7,10 3 2mg/L No.6No.7No.10 3 No6 No.6 No ,8,10 No.10 4, No No.6,7,10 No.2 No.6,7,10 No.2 No6 8,No ,8 No6No mg/L 2 No.6,7,10 8 No.2 6 No.6,7,10 No.2 67
70 COD (mg/l) COD mg/l 68
71 mg/l COD COD No.6,7,10 No.2 20,000 20,000 mg/l 15,000 10,000 5,000 15,000 10,000 5,
72 COD COD 12mg/l COD 2mg/l mg/l COD COD COD COD COD 6.2 COD COD COD COD COD 70
73 6.3 COD 4 10 COD 6.1 COD ,8 COD COD COD 6.4 COD COD 3 1 COD COD 1.5mg/l mg/l COD COD COD 7 2 COD COD COD COD COD 71
74 COD COD COD 1) 27 2) 72
75 COD mg/l mg/l 73
76 mg/l mg/l 74
77 a mg/l 75
78 GC-MS/MSLC-MS/MS GC 28
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2,2-2,2 -Iminodiethanol Diethanol amine CAS 111-42-2 C 4 H 11 NO 2 105.1356-105.0790-28 C 1) 1.0881 g/cm 3 1)
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N,N- N,N-Dimethylacetamide Acethyldimethylamine CAS 127-19-5 C 4 H 9 NO 87.12 ~ 87.14 87.68413 163-165 C 1) - 18.59 C 2) 1 mg/l 25 C 3) 3.3 hpa 2 C 4) log P ow -.77 2).9429 2/4 C 1) 3.1 4) 1.31E - 8 atm-m
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3, ,104 t mg/l 30 mg/l BOD mg 14 C 8, /kg 11 8, 1 11 OH = cm 3 / sec 11 OH /cm ppm
3, 3-1 200019 4800 CAS 91941 1138 3, 3-4, 4 - -3, 3 - H 2 N Cl Cl NH 2 C 12 H 10 C l2 N 2 253.13 1 95 1. 2 3, 4 132-133 402 5 >200 5 700kg/m 3 5 8.73 = 1 5.610-8 kpa 4.210-7 mmhg20 4 6 log Pow3.51 3.57
地域再生計画
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H22応用物理化学演習1_濃度.ppt
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食糧 その科学と技術 No.46( )
, IARC A ppb ppm, WHOµg/L GC GC GCMS GC MS, d O O O NH 2 Br 2 NEt 3 Br NH 2 NH 2 Br Br GC, [C H NO Br] + m/z [C H NO Br] m/z SIM [C H D NO Br] m/z [C H D NO Br] + m/z Br Br HBr [C H NO Br] + m/z GC -d
平成25年度 化学物質分析法開発報告書
2,4-2,4-Dimethylaniline 2,4-2,4-Xylidine CH 3 CH 3 CAS 95-68-1 C 8 H 11 N 121.18 121.0892-14.3 C 2) 214 C 2) 0.9723 g/cm 3 2) 0.133 mmhg (25 C) 1) 1390 mg/l (25 C) 1) 3.710 3 mg/l 25 C WSKOWWIN 5) 6.0710
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030801調査結果速報版.PDF
15 8 1 15 7 26 1. 2. 15 7 27 15 7 28 1 2 7:13 16:56 0:13 3km 45 346 108 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9 3.10 3.11 3. 3.1 26 7 10 1 20cm 2 1 2 45 1/15 3 4 5,6 3 4 3 5 6 ( ) 7,8 8 7 8 2 55 9 10 9 10
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JAJP.qxp
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gc-guide
...1...3 (Direct Injection)...5 OCI...7 PTV (...9 FID...11 ECD...13 AOC-20i/s...15...17...19 10 1 10 1 2µL 1µL 1mL 0.10.2mL He (He) 25cm/sec40cm/sec 30mL/min 1:501:100 Table Ex. Rtx-1, 515m :Ex. 100cm/sec
/ /.. / PCA P レジャー産業と顧客満足の課題
1 1983 1983 2 3700 4800 5500 3300 15 3 100 1000 JR 4 14 2000 55% 72% 1878 2000 5 ( ) 22 1,040 5 946 42 15 25 30 30 4 14 39 1 24 8 6 390 33 800 34 34 3 35 () 37 40 1 50 40 46 47 2 55 4.43 4 16.98 40 55
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Isobutyl alcohol IUPAC 2-methylpropan-1-ol 2--1-2-Methyl-1-Propanol, Isobutanol CH 3 H 3 C OH CAS 78-83-1 C 4 H 10 O log P ow ( C) ( C) (kpa) (g/l) 74.12 74.14 1) -108 2) 108 2) 1.2 2) 87 2) 0.8 2) (74.1214)
埼環協ニュース第229号
General incorporated association Saitama-Prefecture Environmental Measurement Association URL http://www.saikankyo.jp () ( 25 BOD 28 5 810 26 1 10 19.6 7 5.5 2.1 20 7 0 19 7 ( ) 20140131( ) (TSPSPMPM2.5)
AllPrep DNA/RNA Micro プロトコールとトラブルシューティング ( /2008)
July 2007 AllPrep DNA/RNA Micro DNA RNA 5 x 10 5 5 mg Sample & Assay Technologies DNA RNA 3 DNA RNA 11 DNA RNA 19 24 2 AllPrep DNA/RNA Micro 07/2007 DNA RNA RNA AllPrep DNA Spin Column DNA RNeasy MinElute
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土壌の観察・実験テキスト −土壌を調べよう！−
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19 m 3 m 3 m 3 /s m 3 3 m 3 m 3 m 3 m 3 m 3 m 3 m 3 2km 2km 4km 3km 2km 2km 16km () 2km 2km 4km 4km 2km 2km 16km m 3 m 3 m 3 264,970 m 3 1,209.6 m 3 36,850 m 3 1,728 m 3 m 3 m 3 m 3 m 3 m 3 m 3 m
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23 1 23 7 11 (). 2. 3. 4. 22 22 5. 6. 52 59 6 22 1 3o. 2 1) 53.1ppm 1 盤.4ppm.1ppm 3 1 3 盤監 4 たたm. 監 たた m. 監.4.4.3.3.2.2 5.1.1. 4 監盤 7 8 9 1 11 12 1 2 3.1ppm 22 22. 13 14 1 監 1 盤 17 18 19 2 21 22 2) 54.4ppm.6ppm
64 3 g=9.85 m/s 2 g=9.791 m/s 2 36, km ( ) 1 () 2 () m/s : : a) b) kg/m kg/m k
63 3 Section 3.1 g 3.1 3.1: : 64 3 g=9.85 m/s 2 g=9.791 m/s 2 36, km ( ) 1 () 2 () 3 9.8 m/s 2 3.2 3.2: : a) b) 5 15 4 1 1. 1 3 14. 1 3 kg/m 3 2 3.3 1 3 5.8 1 3 kg/m 3 3 2.65 1 3 kg/m 3 4 6 m 3.1. 65 5
98-02.PDF
1 982 2207 CAS 67630 2- H 3 C H 3 C CH OH C 3 H 8 O 60.10 1 99.9 % n- 1. 2-88.5 3 82.5 3 11.7c.c. 455 4 212 d 20 4 0.78505 3 2.1 = 1 4 4.4 kpa 33 mmhg20 4 log Pow0.05 0.07 5 m/z45, 1.0 43 0.19 27 0.17