180525_Nucleofector_Technology_冊子改訂【2校】.indd
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1 Bioscience Solutions Nucleofector Nucleofector Technology for Hard-to-transfect Cells
2 D-Nucleofector LV 4D-Nucleofector LogWare Nucleofector PLUS 4D-Nucleofector Y Nucleofection 4D-Nucleofector HT Nucleofector Nucleofector Nucleofector well Nucleofector 96-well Shuttle 100 Nucleofector Nucleofector Nucleofection Nucleofector II 2 Nucleofector 15 Nucleofector Nucleofector Nucleofector 2
3 2 3 Nucleofector 4 5 Nucleofector 6 4D-Nucleofector 7 4D-Nucleofector X 8 Nucleofection 4D-Nucleofector Y 9 4D-Nucleofector LV well Shuttle 11 HT Nucleofector 12 Nucleofector 2b 13 4D-Nucleofector
4 Nucleofector 1998 Nucleofector 2001 Nucleofector 2 4D-Nucleofector HT Nucleofector 21 CFR part11 / annex11 4D-Nucleofector LogWare Nucleofector Nucleofector 競合他社 Bエレクトロポレーション SSC SSC 30.31% 2.75% GFP GFP 1 Nucleofector NK NKL NKL pmaxgfp 2.5 µg Nucleofector VO-017 B 25 mv 96 µf 24 Nucleofector 18 Nucleofector John Coligan, Laboratory of Immunogenetics, NIH/NIAID J Immunol Methods (2004 ) Nucleofector 99% DNA A B C D µg egfp GFP TMR DNA2 3.5 PFA TMR(A) GFP (B) DAPI(C) 3 (D) 4
5 Nucleofector Cas9 plasmid Nuclease sirna 4000 % 導入効率 hes Cell line H9 hes Cell line BG01V hes Cell line H9.2 hes Cell line HUES 9 Human MSC Human NSC Mouse NSC Rat hippocampal neurons Rat NSC Rat cortical neurons Mouse glial cells Human T-cells (unstimulated) HUVEC PBMC 3 Nucleofector Human Pre Adipocytes Rat cardiomyocytes D-Nucleofector DNA RNAPNA H9 ESpmaxGFP Nucleofection H9 24(A) GFP SSEA4 Oct4 DAPI Jennifer Moore, RutgersUniversity, Piscataway, USA 5
6 Nucleofector Nucleofector Nucleofector Nucleofector Nucleofector PC 3 Nucleofector Nucleofector pmaxgfp Nucleofector Nucleofector Nucleofector Nucleofector ector Nucleofector 4D-Nucleofector 96-well Shuttle LV HT Nucleofector Nucleofector 2b () (1-16) (1-96) (1) (384) (1) 20 µl µl 20 µl 1 ml, 20 ml 20 µl 100 µl (20 µl) 2 x x x x 10 6 ( )1 x x 10 9 (1 ml) 2 x x 10 6 (100 µl) 2 x x x x 10 7 ( )1 x x 10 9 (20 ml) DNA µg (20 µl) 1 5 µg (100 µl) µg µg(1 ml) µg 1 5 µg 20 µl Nucleocuvette well Shuttle AAF-1002B ( ) AAF-1002X ( X ) AAF-1002Y ( Y ) AAM-1001S AAF-1002L AAU-1001 AAB ,000,000 X 1,450,000 Y 1,700,000 2,400,000 1,050,000 6
7 4D-Nucleofector 4D-Nucleofector 4D-Nucleofector 1 X 1 X 4D-Nucleofector 1 2 USB 3 4D-Nucleofector 100 µl 20 µl µl Nucleocuvette µl Nucleocuvette 1 4D-Nucleofector µl Nucleocuvette 100 µl Nucleocuvette 5020 µl Nucleocuvette well Shuttle LV 1 ml 20 ml cm 5 USB 96-well Shuttle USB 2 X Nucleofection 20 µl Nucleocuvette 100 µl Nucleocuvette Nucleofection 20 µl 100 µl 96-well Shuttle 3 Y
8 4D-Nucleofector X 4D-Nucleofector X 2Nucleocuvette 100 µl Nucleocuvette 100 µl 20 µl 16-well Nucleocuvette 96-well Nucleocuvette RNAi 100 µl 20 µl Nucleocuvette 1 4D-Nucleofector 96-well Shuttle HT Nucleofector 96-well Shuttle 4D-Nucleofector (%) μl 100 μl 20 μl 100 μl 20 μl 100 μl 20 μl 100 μl 20 μl 100 μl Human T Cell (FI-115) Human T Cell (EO-115) HUVEC (CA-167) NHDF-neo (DT-130) Rat Cortex Neurons (CU-133) 導入効率 生存率 5 2Nucleocuvette 20 µl 100 µl 24 8
9 4D-Nucleofector Y Nucleofector 4D-Nucleofector Y % Clonetics 4D-Nucleofector Y AD1AD2Nucleofector D- poly-d-lysine PDL AD1 4D-Nucleofector Y pmaxgfp 1MAP2 maxgfp HUVEC 神経細胞またはグリア細胞 内皮細胞 その他の細胞 神経細胞向けの基本プロトコル 内皮細胞向けの基本プロトコル 最適化プロトコル 最適化 4D-Nucleofector Y キット AD1 4D-Nucleofector Y キット AD2 4D-Nucleofector Y キット 7 HUVEC ,000 1AD1 4D-Nucleofector Y CA µg pmaxgfp 24 maxgfp M. Sauvage FR 9
10 LV New 4D Nucleofector X X 4D Nucleofector LV X LV T CHO-S, HEK293-S K D-Nucleofector LogWare 21CFT part11 8 4D-Nucleofector LV 2 ipsc, CD34, T Ex-vivo Sleeping Beauty PiggyBac T CAR T CHO-S, HEK293-S 1 ml Nuclocuvette 1 ml 1 10 LV Nuclocuvette 20 ml Volume Cells 100 µl 5x ml 3-5x µl 1x ml 1x µl 1x ml 1x µl 5X ml 1x µl 5x ml 1x10 9 Human T cells K562 HL-60 CHOs (Lonza clone) FreeStyle 293-F Efficiency (%) Viability (Pl neg cells; %) 9 pmaxgfp 10
11 96-well Shuttle 96-well Shuttle 4D-Nucleofector 3 4D-Nucleofector X 96-well Shuttle 96-well Nucleocuvette 96-well Shuttle P1-P5 96-well Nucleofector SE, SF, SG 96-well Nucleofector / Nucleocuvette % 導入効率 T A20 ARPE-19 BHK-21 BT549 BV2 Cal-85-1 Calu-3 CAMA 1 CHO-DG44 (DHFR-) Cor.At COS-7 DU 145 EL4 GH3 GM00131 H4 HCC1954 HCT 116 HDQ-P1 HEL J-774 KE-37 L5178Y MC/9 MCF10A MDA-MB-231 MDA-MB-415 MDCK mimcd3 PtK1 RIN m5f SET-2 Sf9 SK-MEL-5 SK-N-MC T-47D UACC well Nucleofector 11
12 HT Nucleofector HT Nucleofector Nucleofector 96-well Shuttle HT Nucleofector 3 2 HT Nucleofection HT Nucleofector 96-well Shuttle 384 Nucleocuvette SBS Well Shuttle HT Nucleofector (%) x10 4 Nucleofection 5 µl 96-well Shuttle Cho-K1 HeLa-S3 HUVEC Jurkat NIH-3T3 導入効率 HT Nucleofector システム 導入効率 96-well Shuttle システム 生存率 HT Nucleofector システム 生存率 96-well Shuttle システム well Shuttle HT Nucleofector HT Nucleofector 96-well Shuttle HT Nucleofector 12
13 Nucleofector 2b Nucleofector 2b 2001 DNA sirna 50 5 DNA 90% sirna 99% D A20 HL-60 Jurkat K-561 KG-1a Raji RAW RBL-1 T2 THP-1 U-937 WEHI-231 Nucleofector egfp GFP maxgfpr H-2K K % 13
14 4D-Nucleofector 4D-Nucleofector, 96-well Shuttle 384-well Nucleofector 4D-Nucleofector, 96-well Shuttle, HT Nucleofector 96-well Shuttle Nucleofector Lonza 4D-Nucleofector 5 5 4D-Nucleofector 4D-Nucleofector Nucleofector 4D-Nucleofector, 96-well Shuttle, 384-well Nucleofector SE, SF, SG 3Nucleofector 100 µl Nucleocuvette 16-well Nucleocuvette 4D-Nucleofector X 4D-Nucleofector X 4D-Nucleofector Y RNAi / 2 x x x x x µl 20 µl 350 µl Table 1 4D-Nucleofector, 96-well Shuttle, HT Nucleofector 14
15 4D-Nucleofector Y 24 4D-Nucleofector Y Nucleofector II/2b Nucleofection Nucleofector II/2b Nucleofector Nucleofector 5 Nucleofector C L R T V Nucleofector Nucleofector 4D-Nucleofector Y Nucleofector 2 Nucleocutor Y Nucleofector II/2b LV X SF P3 2 LV unit Nucleocuvette 96-well Nucleocuvette 384-well Nucleocuvette LV 96-well Shuttle 384-well Nucleofector Nucleofector II/2b CAR T RNAi / 1 x 10 1 x 10 2 x x x x ml20 ml 20 µl 20 µl 100 µl 1 ml 2 20 ml
16 Nucleofector 4D-Nucleofector AAF-1002B 1,000,000 4D-Nucleofector X AAF-1002X 1,450,000 4D-Nucleofector Y AAF-1002Y 1,700, well Shutte add-on (PC ) AAM-1001S 2,400,000 X 4D-Nucleofector LV AAF-1002L 4D-Nucleofector LogWare SAAF-1001 HT Nucleofector AAU-1001 HT Nucleofector AWT-1001 Nucleofector 2b AAB ,050,000 4D-Nucleofector Core 1 AWA-3001-B 4D-Nucleofector X 1 AWA-3001-X 4D-Nucleofector Y 1 AWA-3001-Y 4D-Nucleofector LV 1 AWA-3001-LV 96-well Shutte Device 1 AWA-3001-S Nucleofector 2b Device 1 AWA b HT Nucleofector 1 AWA-3001-HT 100 μl Nucleocuvette 20 μl Nucleocuvette ; 16-well Dipping Electrode D-Nucleofector P 4D-Nucleofector X V4XP ,000 V4XP ,400 V4XP ,200 P 4D-Nucleofector X V4XP ,000 V4XP ,400 V4XP ,200 P 4D-Nucleofector X V4XP ,000 V4XP ,400 V4XP ,200 P 4D-Nucleofector X V4XP ,000 V4XP ,400 V4XP ,200 P 4D-Nucleofector X V4XP ,000 V4XP ,400 V4XP ,200 4D-Nucleofector X, 96 V4XP ,000 4D-Nucleofector X, 64 V4XC ,000 AD 4D-Nucleofector Y () V4YP-1A24 58,000 AD2 4D-Nucleofector Y () V4YP-2A24 58,000 4D-Nucleofector Y, 48 V4YP-9A48 116,000 SE4D-Nucleofector X V4XC ,000 V4XC ,400 V4XC ,200 SF4D-Nucleofector X V4XC ,000 V4XC ,400 V4XC ,200 SG4D-Nucleofector X V4XC ,000 V4XC ,400 V4XC ,200 1 ml Nucleocuvette Cartridge 20 ml Nucleocuvette Cartridge D-Nucleofector LV SF4D-Nucleofector LV L / LV V4LC-2002 V4LC-2020 V4LC-2520 P 4D-Nucleofector LV L / LV V4LP-3002 V4LP-3020 V4LP
17 20 μl Nucleocuvette ; 96-well well Shuttle P 96Nucleofector V4SP ,800 P 96Nucleofector V4SP ,000 P 96Nucleofector V4SP ,000 P 96Nucleofector V4SP ,000 P 96Nucleofector V4SP ,000 96Nucleofector (160 ) V4SP ,000 SE96Nucleofector V4SC ,800 SF96Nucleofector V4SC ,800 SG96Nucleofector V4SC , µl Aluminum Cuvette Nucleofector II/2b B Nucleofector VAPA ,000 VPA ,500 T Nucleofector VAPA ,000 VPA ,500 CD34+ Nucleofector VAPA ,000 VPA ,500 Nucleofector VAPA ,000 VPA ,500 Nucleofector VAPA ,000 VPA ,500 T Nucleofector VPA ,500 Nucleofector VPA ,500 Nucleofector VAPA ,000 VPA ,500 Nucleofector VAPA ,000 VPA ,500 B Nucleofector VAPA ,000 VPA ,500 Nucleofector VAPA ,000 VPA ,500 / Nucleofector VAPF ,000 VPF ,500 Nucleofector VAPE ,000 VPE ,500 Nucleofector VAPD ,000 VPD ,500 NHEM-Neo Nucleofector VAPD ,000 VPD ,500 HCAEC () Nucleofector VAPB ,000 VPB ,500 HUVEC ( ) Nucleofector VAPB ,000 VPB ,500 HMVEC-L () Nucleofector VAPB ,000 VPB ,500 Nucleofector VAPI ,000 VPI ,500 Nucleofector VAPI ,000 VPI ,500 17
18 µl Aluminum Cuvette Nucleofector II/2b Nucleofector VAPD ,000 MEF Nucleofector VAPD ,000 VPD ,500 MEF Nucleofector VAPD ,000 VPD ,500 MEF Nucleofector VPD ,500 Nucleofector VAPI ,000 VPI ,500 /Nucleofector VAPL ,000 VPL ,500 Nucleofector VAPG ,000 VPG ,500 Nucleofector VAPG ,000 VPG ,500 Nucleofector VAPG ,000 VPG ,500 Nucleofector VAPI ,000 VPI ,500 Nucleofector VAPI ,000 VPI ,500 Nucleofector VAPC ,000 VPC ,500 Nucleofector VAPI ,000 VPI ,500 CD34+ Nucleofector VAPA ,000 VPA ,500 (MSC) Nucleofector VAPE ,000 VPE ,500 Nucleofector VAPH ,000 VPH ,500 Nucleofector VAPH ,000 VPH ,500 Nucleofector 18 VPH ,500 ES Nucleofector VAPH ,000 VPH ,500 (NSC) Nucleofector VAPG ,000 VPG ,500 (NSC) Nucleofector VAPG ,000 VPG ,500 Nucleofector 1 VAMI ,000 VMI ,500 Nucleofector 2 VAMI ,000 VMI ,500 Nucleofector VMI ,500 Cell Line Kits Nucleofector R VACA ,500 VCA ,500 Nucleofector T VACA ,500 VCA ,500 Nucleofector V VACA ,500 VCA ,500 Nucleofector C VACA ,500 VCA ,500 Nucleofector L VACA ,500 VCA ,500 Nucleofector 18 VCO-1001N 48,000 18
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20 Lonza Cologne GmbH Cologne, Germany For research use only. Not for use in diagnostic procedures. The Nucleofector Technology is covered by patent and/or patent pending rights owned by the Lonza or its affiliates. All trademarks belong to Lonza or its affiliates or to their respective third party owners. The information contained herein is believed to be correct and corresponds to the latest state of scientific and technical knowledge. However, no warranty is made, either expressed or implied, regarding its accuracy or the results to be obtained from the use of such information and no warranty is expressed or implied concerning the use of these products. The buyer assumes all risks of use and/or handling. Any user must make his own determination and satisfy himself that the products supplied by Lonza Group Ltd or its affiliates and the information and recommendations given by Lonza Group Ltd or its affiliates are (i) suitable for intended process or purpose, (ii) in compliance with environmental, health and safety regulations, and (iii) will not infringe any third party s intellectual property rights Lonza. All rights reserved. CD-BR005 02/18 RES-LF PNT
遺伝子導入 Nucleofector 152 Nucleofector 15 D-Nucleofector 155 Nucleofection 156 Nucleofector D-Nucleofector Nucleofector 2b Nucleofector 16
バイオリサーチ 遺伝子導入 Nucleofector 151 Nucleofector 157 Nucleofector 163 Nucleofector 225 遺伝子導入 Nucleofector 152 Nucleofector 15 D-Nucleofector 155 Nucleofection 156 Nucleofector D-Nucleofector 158 159 Nucleofector
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