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1 Light sheet Microscopy for Multiview Imaging of Large Specimens
2 Be Amazed at Where Life Can Take You! Lightsheet Z BACGFP 3D csp cystein string protein BAC H.BellenM.Weber P.Tomancak MPI- CBG, Dresden, Germany 2
3 Simpler. More intelligent. More integrated. Carl Zeiss Z- Z- CO 2 3
4 Your insight into the Technology Behind It 最大の光量子効率と最高の速度 ライトシート蛍光検鏡 LSFM では蛍光の励起と検出が Illumination 2 つの異なる光路に分けられます 励起する照射軸は 検出軸と垂直になります すなわち 試料の一枚の極めて とは 特長 アプリケーション システム構成 技術仕様 サービス 薄い領域を一度に照射できるので 焦点面の蛍光のみを Light sheet Focal overlap 励起することで固有の光学セクショニングを作成できる のです ピンホールや画像処理は必要ありません 焦点面 からの光は 共焦点や他のレーザスキャン顕微鏡のように 画素単位での検出ではなく カメラのピクセルに一度に x 集められます カメラベースの検出器で画像情報を並列 Detection 処理で集めることで より早く そして他の顕微鏡シス テムで使うより少ない励起光でイメージングが可能に Detection Optics z なります LSFMは 光学セクショニング効果と焦点面か らの並列処理による画像取得を組み合わせています これは 3D イメージングを極めて高速にし かつ励起 効率も高めることができます y z x ライトシート蛍光検鏡 LSFM の原理 図は Jörg Ritter氏の博士論文 ボン大学 ドイツ 2011年 より 4
5 Tailored Precisely to Your Applications / C. 3D 3D 5
6 at Work fps 2 24 Tg Bactin:H2A-mCherry 6 2 3DMIP Maximum Intensity Projection: 80fps M. Weber J. Huisken MPI-CBG Dresden, Germany
7 at Work. Parhyale hawaiensis Histone-RFP 2 4 :W Plan- Apochromat 20x/1.0:30ms:561nm 400 Parhyale hawaiensis4 Histone2BmRFPruby 3D C. Staber J. Zeitlinger Stowers Institute for Medical Research, Kansas City, USA 3TBZEN A.Pavlopoulos P.Tomancak MPI-CBG Dresden, Germany 7
8 Flexible Choice of Components CO2 3. 5x/0.1 10x/ nm 445nm 488nm 515nm 561nm 638nm LED 5. ZEN 2012 black edition Multiview Processing 3D VisArt 2. 5x/ x/1.0 40x/1.0 63x/ Standard Highend 8
9 System Overview Highend LBF LCD TFT 30 9
10 5x/0.1 10x/0.2 LED 405 nm 445 nm 488 nm 515 nm 561 nm 638 nm Standard ICX 285 CCD Highend scmos, x/ x/1.0 40x/1.0 63x/1.0 Multiview Processing 3D VisArt 8 TB 32 TB TempModule S1 TempModule S1 TempModule CZ-LSFM CO2 10
11 800 mm 450 mm 500 mm 75 kg LB Rack Lightsheet 600 mm 700 mm 550 mm 80 kg 900 mm 750 mm 770 mm 90 kg IR LED / / nm x 2.5x 0.7x 2.5x 0.36x 0.7x 60 m 2.8 mm 2.8 mm 5x 0.7x 0.36 x >5 mm < 1 m 5 mm 2 m 14 m 488 nm 11
12 Standard 2 CCD Sony ICX 285 / C 6.45 m AD Highend 14 bit > 10 fps Z scmos/ C 6.5 m Mpixel AD 15 bit 30 fps Z 150 Mbyte / 42 C 0.5 / 10 C 42 C 1.5 / 1.0 / 0.1 C CO 2 CO2 0 % 10 % 12
13 4 x / y / z / 10 mm / 50 mm / 10 mm / nm / 650 nm / 200 nm / nm / 1 m / 50 nm / / Z 2 mm / 3B nm 20 mw 50 mw 445 nm 25 mw 40 mw 488 nm 30 mw 50 mw 100 mw 515 nm 20 mw 50 mw 561 nm 20 mw 50 mw 75 mw 638 nm 75 mw 13
14 22 C 3 C 15 C 35 C 30 C < 65% 2000 m 60 / 3 h C VC-C8 80 HzRMS 12,5 m /s RMS = root mean square / ISO V AC 240 V AC 10% 100 V AC 125 V AC 10% Hz Hz 3.5 A 8 A 800 VA 750 VA PC 600 VA 600 VA / I / IP 20 EMC II DIN EN CISPR 11/ DIN EN PC 700 W 600 W US US US US US EP
15 Count on Service in the True Sense of the word 15
16 facebook.com/zeissmicroscopy twitter.com/zeiss_micro youtube.com/zeissmicroscopy flickr.com/zeissmicro Printed in Japan P13 MIC 023 PR SK-12/ TM Tel Fax
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