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1 RNA- RNA RNA Nanotechnology & Therapeutics Lexington RNA Salt Lake City FNANO FNANO RNA RNA RNA- RNP RNA DNA DNA DNA RNA RNA ips CiRA RNA-Protein nanotechnology & synthetic biology Hirohide Saito The Hakubi Center for Advanced Research & Center for ips Cell Research and Application CiRA, Kyoto University, Kawahara-cho, Shogoin, Sakyo-ku, Kyoto, Japan RNA RNA RNP RNA RNP RNA RNA DNA Nature DNA DNA DNA staple DNA DNA RNA RNA RNA RNP RNA Luc Jaeger tecto RNA RNA tecto RNA RNA RNA Jaeger tecto RNA RNA RNA Pexian Guo RNA prna-way junction RNA Guo RNA prna RNA sirna pp.

2 RNA in vivo RNA RNA prna sirna EGFR ips prna NCI Bruce Shapiro Kirill Afonin RNA DNA-RNA RNA DNA-RNA RNAi RNA RNA RNA RNA RNA RNP RNP RNP L Ae RNA RNA RNA http: biocentre nasg kturn RNA L Ae RNP nm koff s L Ae L Ae RNA L Ae RNA RNP Discovery Studio RNP L Ae RNA RNA AFM RNA RNA L Ae

3 L Ae RNP RNA AFM L Ae L Ae RNA RNP RNA L Ae RNA- RNP RNP RNP RNP RNA- mrna trna mrna -UTR RNA- RNA RNA RNA RNA RNA RNA

4 L Ae RNA mrna -UTR RNA L Ae mrna RNA mrna L Ae L Ae mrna mrna L Ae RNA mrna L Ae RNP RNP L Ae mrna L Ae RNP RNA mrna RNA RNP RNA RNA RNP RNA RNA RNP RNA Jim Stapleton ips ICORP NEDO Saito, H. Inoue, T.Int. J. Biochem. Cell Biol.,,. Rothemund, P.W.Nature,,. Guo, P.Nat. Nanotechnol.,,. Jaeger, L. Leontis, N.B.Angew. Chem. Int. Ed. Engl.,,. Chworos, A., Severcan, I., Koyfman, A.Y., Weinkam, P., Oroudjev, E., Hansma, H.G., Jaeger, L.Science,,. Nasalean, L., Baudrey, S., Leontis, N.B., Jaeger, L. Nucl. Acids Res.,,. Shu, D., Shu, Y., Haque, F., Abdelmawla, S., Guo, P. Nat. Nanotechnol.,,. Afonin, K.A., Viard, M., Martins, A.N., Lockett, S.J., Maciag, A.E., Freed, E.O., Heldman, E., Jaeger, L., Blumenthal, R., Shapiro, B.A.Nat. Nanotechnol.,,. Klein, D.J., Schmeing, T.M., Moore, P.B., Steitz, T.A. EMBO J.,,. Ohno, H., Kobayashi, T., Kabata, R., Endo, K., Iwasa, T., Yoshimura, S. H., Takeyasu, K., Inoue, T., Saito, H. Nat. Nanotechnol.,,. Saito, H., Kobayashi, T., Hara, T., Fujita, Y., Hayashi, K., Furushima, R., Inoue, T.Nat. Chem. Biol.,,. Saito, H., Fujita, Y., Kashida, S., Hayashi, K., Inoue, T. Nat. Commun.,,. Stapleton, J. A., Endo, K., Fujita, Y., Hayashi, K., Takinoue,

5 M.,Saito,H., Inoue, T.ACS Synth. Biol.,,. Murata, S., Konagaya, A., Kobayashi, S., Saito, H., Hagiya, M.New Gener. Comput.,,. ips CiRA DC SPD ips RNA saito

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