Microsoft Word - RMD_75.doc
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1 Review Multi Dimensional Data Multi Dimensional Acquire *.nd MetaMorph.nd Muliti Dimensional Acquisition Stack Tiff Multi Dimensional Acquisition Z nd nd 1 Review Multi Dimensional Data nd Review Multi Dimensional Data nd nd nd MetaMorph StackTiff nd Molecular Devices Japan KK/ Imaging Team (1/15)
2 1. Apps Review Multi Dimensional Data Review Multi Dimensional Data 2. Multi Dimensional Data Set Utilities Molecular Devices Japan KK/ Imaging Team (2/15)
3 3. Browse for Folder OK 4. Data Sets*.nd Data Sets Z Molecular Devices Japan KK/ Imaging Team (3/15)
4 5. Review Multi Dimensional Data Wavelengths Wavelengths MetaMorph Illumination MetaMorph 6. Time Z Time 1set Z 1set Molecular Devices Japan KK/ Imaging Team (4/15)
5 7. Selections[X s] Clear 8. Z MetaMorph Select Best Focus 1 Z ROI Select Best Focus Molecular Devices Japan KK/ Imaging Team (5/15)
6 9. Display ROI Active Region Full Image Source R:G:B:Gray Color Composite RGB() Z Z Projection Z ProjectionZ TypeZ Projection Maximum Minimum AngleZ Projection 90 PlanesZ Projection All Plane Z Projection OrientationAngle Border ColorAngle Create Rotation Z DistanceZ User Specified (m) Z Projection Molecular Devices Japan KK/ Imaging Team (6/15)
7 11. Enable Montage # of Thumbnails MetaMorph Multi Dimensional Acquisition Enable Montage Multi Dimensional Acquisition Review Multi Dimensional MetaMorph Version7.1 Multi Dimensional Acquisition Review Multi Dimensional nd Molecular Devices Japan KK/ Imaging Team (7/15)
8 Multidimensional Acquisition Event Type Color Molecular Devices Japan KK/ Imaging Team (8/15)
9 Event Type,Color Molecular Devices Japan KK/ Imaging Team (9/15)
10 MetaMorph Review Multidimensional Data 1.Main Review Multidimensional Data Open Sequential Image As Multi Dimensional Image Browse File Name Convention(Ex:***) Settings Open Sequential Image + seq Review Multi Dimensional Data Next 2.Dimension Info Molecular Devices Japan KK/ Imaging Team (10/15)
11 2-1.Dimension Info File Name Convention Simple Sequence Dimension Sizes Image Name increments Image Range To Open Molecular Devices Japan KK/ Imaging Team (11/15)
12 Simple Sequence Increment Simple Sequence () Order of dimensions in selected file 12 TIFF GFP RFP Z File001.tif GFP Z=1 T=1 File002.tif GFP Z=2 T=1 File003.tif GFP Z=3 T=1 File004.tif RFP Z=1 T=1 File005.tif RFP Z=2 T=1 File005.tif RFP Z=3 T=1 File006.tif GFP Z=1 T=2 File007.tif GFP Z=2 T=2 File008.tif GFP Z=3 T=2 File009.tif RFP Z=1 T=2 File010.tif RFP Z=2 T=2 File011.tif RFP Z=3 T=2 Dimension increment Z increment (Z=3) GFP RFP (Wavelength(W)=2) ZWT increment(t=2) Dimension Dimension Next 3.Dimension Properties Molecular Devices Japan KK/ Imaging Team (12/15)
13 2-2. Dimension Info File Name Convention Complex Sequence Increment By Increment Image Increment Plane stack Increment ( Stack ) Name Increment By Image Changes By Increment By Plane Increment Next 3.Dimension Properties Molecular Devices Japan KK/ Imaging Team (13/15)
14 3. Dimension Properties Open Sequence Image Wavelength Color Wavelength Image Stage Label Add Stage Labels Z DistanceZ Z (m) Next 4.Preview Molecular Devices Japan KK/ Imaging Team (14/15)
15 4.Preview Preview OK 5. File Multi Dimensional Tools Open Sequential As Multi Dimensional Image Molecular Devices Japan KK/ Imaging Team (15/15)
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