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1 TEM with CCD Ver FILAMENT OFF 80 V () FILAMENT 2 25 FILAMENT OFF FILAMENT 1 min 2-5 L TEM(&CCD) FILAMENT ON CCD( ) - 1 -
2 3 TEM 3 CCD (CCD ) 7 CCD CCD &TEM
3 1. p3 return PAGE3 ( ) PAGE 3 P1: 28 2: 28 3: 28 4: 5: TEM 1. () 2. ( ) 3. >htset_80 return ok htset[80] 4. HT () 80 kv COND. STIG SPOT SIZE BEAM CURRENT 40 BIOS OBJ. STIG 5. >load_ht return >run return start ht?(kv) 80 return stop ht?(kv) 200 return ht step?(kv 10) 1 return time limit?(min) 120 return HT ACCEL VOLTAGE kv BRIGHTNESS FILAMENT : : : BRIT. TILT SHIFT X DEF X CCD 6. CCD CCD ON cool / off / warm OFF COOL 25 o C Auto - 3 -
4 Open Auto 8. Heat Sink cool Reset TEM 15 cm 9. (1kV/s ) DOWN BEAM CURRENT DOWN (TEM ) (!!) 2 2 1!!!! 12. AIR PUMP ( ) p3 return PAGE3 ( ) P4 32 ( 5 ) cm cm P P1: 28 2: 28 3: 28 4: 35 5:
5 p1 p5 18. FILAMENT FILAMENT ON 4 BEAM CURRENT +1, 2, 3 ( 6) BIOS BIOS ) (kv) BEAM CURRENT * FILAMENT * BIAS * LOW MAG GUN+SHIFT X or Y * MAG1 SELECTOR FOCUS SHIFT Y DEF Y MAG2 LOW MAG WOBBLER HT IMAGE X or Y : : DIFF SCREEN SHUTTER PHOTO SHIFT X or Y GUN K (MAG2 ) FOCUS PAGE1 DV 0 2. Z DEF X or Y 3. GUN DEF x DEF y SHIFT x SHIFT y - 5 -
6 CRT BRIGHTNESS (X and Y ) 5. SpotSize 1 BRIGHTNESS 6. GUN SHIFT x SHIFT y() Y X 7. SpotSize 5 BRIGHTNESS 8. BRIGHT-SHIFT x SHIFT y () BRIGHTNESS COND. STIGM. DEF x DEF y 11. SpotSize 1 1. Mag1 LOW MAG ( ) 2. WOBBLER-IMAGE X IMAGE Y FOCUS ( FOCUS ) 3. WOBBLER-HT BRIGHT TILT DEF x DEF y WOBBLER-HT OFF (2-3 ) 4. DIFF (3 ) MAG1-6 -
7 OBJ STGM DEF x DEF y over under TEM 4. WOBBLER-STIGM BRIGHTNESS SHIFT (CCD ) PHOTO AUTO 3. 2s 4s PHOTO PHOTO 6. PHOTO ( ) 7. CCD 1. ON OFF COOL (TEM ) CCD TEM K CCD Gatan Digital Micrograph 5. Image info Global info Specimen, operator CameraView(search) 6. CameraView search Autoexplosure - 7 -
8 Focus loupe Auto Survey Camera inserted 7. TEM SCREEN 8. CameraView Start camera 9. Yes 10. magnification voltage TEM mode imaging 11. OK 12. value ( 10000) Brightness start view 13. stopview 14. Advanced setting Process: Gain Normalized Bining Setup: Search/Focus Search 2 Focus Bining 1 CameraView(focus) 15. CameraView focus search 16. Focus loupe CameraAquire 17. Binning 1 AdvancedSetting 18. Start Acquire 19. magnification voltage TEM mode imaging 20. OK - 8 -
9 CCD 1. Display control 2. Edit menu Data Edit Add scale bar 3. tool 4. standard tool 5. Target Page Save 10 Voltage, Magnification 6. File menu Save As Gatan 16 MB/ CD-R User Data 7. TIFF, JPEG File menu Save Display As TEM CCD 1. camera inserted 2. TEM Screen 3. 20k FILAMENT FILAMENT ON - 9 -
10 1. CCD Camera Insert 2. SCREEN 3. BEAM CURRENT FILAMENT FILAMENT ON FILAMENT cm 15 1cm 15() TEM PUMP AIR AIR PUMP ( ) p3 return PAGE3 ( ) 14. P4 32 ( 5 ) cm cm 16. FILAMENT ON FILAMENT ON FILAMENT BEAM CURRENT 2. FILAMENT ON ()FILAMENT OFF CAMERA ( ) ( ) 7. CAMERA 8. CAMERA
11 >fun return >50 return TEM 7. EVAC CCD &TEM 1. CCD Camera Insert 2. SCREEN 3. Gatan Digital Micrograph 4. CCD cool off TEM OFF 5. CCD FIRST LIGHT OFF 6. OFF ON 7. PAGE P3 28 ( ) 11. FILAMENT BEAM CURRENT 12. FILAMENT ON ()FILAMENT OFF cm 10 10cm 15( ) TEM
12 11. N PUMP AIR Accelerator voltage () down BEAM CURRENT down 80kV () HT ( )OFF 16. ACD () keitatcm@mbox.nc.kyushu-u.ac.jp
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