感染症学雑誌第80巻第5号
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- しょうすけ いしなみ
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1 Shigella Shigella sonnei S. sonnei
2 S. sonnei Campylobacter Staphylococcus aureus S. sonnei S. boydii S. flexneri S. dysenteriae µ µµ Mse µµ Eco µ µ µ µ µ µ µmse µeco µ µ Mse Eco µ µ µ µ µeco µmse µ µ
3 Table1 Typingresultsforeachisolateorstrainusingcolicintyping,pulsed-fieldgelelectrophoresis,andamplifiedfragmentlengthpolymorphism No. Spices Yearof Isolation Originoratthemakingof apassagedestination Colicintyping Pulsed-fieldgel electrophoresis Amplifiedfragment lengthpolymorphism S.boydi S.boydi S.boydi11 S.boydi1 S.boydi13 S.flexneri S.flexneri 3a S.flexneri-93 S.dysenteriae ATCC929 ATCC11 ATCC2929 ATCC293 ATCC2931 DomesticoutbreakinJapan SameascaseNo. SameascaseNo. SameascaseNo. SameascaseNo. SameascaseNo. SameascaseNo. SameascaseNo. SameascaseNo. SameascaseNo. andsingapore Domesticsporadicinfection Indonesia Vietnam Indonesia Singapore Egypt SouthKorea andnepal Russia China MexicoandPanama ThePhilippines BaliinIndonesia ATCC1227 ATCC122 ATCC1229 ATCC123 ATCC1232 BaliinIndonesia A 9A µ µ µ Ecoµ Mse µ µ
4 Eco Mse S. sonnei S. boydii Eco Mse µ µ µ µ Xba S. sonnei S. sonnei S. sonnei S. sonnei S. sonnei
5 Fig.1 Amplifiedfragmentlengthpolymorphism paternsanddendrogram of1shigelaisolates.the dendrogram wasconstructedwithfingerprinting I(BioRad)byunweightedpair-groupmethodusingaveragelinkage.ThepercentageofsimilaritycalculatedbytheDicecoeficientisshownabove thedendrogram. S. flexneri S. flexneri S. flexneri S. boydii
6 Table2 Evaluationoftyping Typing Typeability *1 Reproducibility *2 Discriminatorypower *3 Easeofinterpretation *4 Easeofperformance * Colicin PFGE AFLP NT * Excelent Excelent Good Excelent Good Good *1 Proportion(%)ofstrainstypeable. *2 PercentageofsimilaritycalculatedbyDicecoeficientamongeachofthreestrainsintriplicate. *3 Simpson sindex. 14) N:totalnumberofstrainsinthesamplepopulation. N N nj nj S j S :totalnumberoftypesdescribed. nj:numberofstrainsbelongingtothejthtype. *4, * Thesejudgmentsrepresenttheviewsoftheauthors.Seethetextfordetails. * Nottested. Table3 Reproducibilityofpulsed-fieldgelelectrophoresisandamplifiedfragmentlength polymorphism ATCC929 Trial Third Pulsed-fieldgelelectrophoresis ATCC *1 929 First 1 *2 1 1 ATCC2929 First ATCC2931 First Amplifiedfragmentlengthpolymorphism ATCC929 First ATCC2929 First ATCC2931 First *1 AmericanTypeCultureColection. ATCC2929 Third ATCC2931 Third *2 PercentageofsimilaritycalculatedbyDicecoeficient, Dicecoeficient:2n XY/(n X+n Y) n X:totalnumberofDNA fragmentsfrom isolatex. n Y:totalnumberofDNA fragmentsfrom isolatey. n XY:totalnumberofDNA fragmentsidenticalintwoisolates.
7
8 S. sonnei S. sonnei S. flexneri Shigella dysenteriae Shigella sonnei Shigella Shigella sonnei et al. Shigella sonnei et al. Campylobacter
9 Staphylococcus aureus Salmonella Shigella flexneri et al. Acinetobacter baumannii Legionella pneumophila Aeromonas et al. Shigella Shigella Shigella Shigella Shigella Shigella
TEL:03-5253-1111 2478 TEL:03-3595-2337 FAX:03-3503-7964 Shigella sonnei 200g 300 500cm 0.2 0.3mm 25g 0.45 m Shigella sonnei 3mL 35 37 18 ml 0.1 ml 10 10 ml 40mL 0.1 200 L 25g 1 225mL 35 37 18 24 Shigella
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