日本感染症学雑誌第82巻第3号
|
|
|
- うまじ うすい
- 6 years ago
- Views:
Transcription
1 Mycoplasma peumoiae Mycoplasma peumoiae Mycoplasma peumoiae SDC M. peumoiae M. peumoiae M. geitalium Mycoplasma Mycoplasma peumoiae
2 M. peumoiae M. peumoiae M. peumoiae MycoplasmaM. geitalium M. homiis M. salvarium M. fermetas M. orale M. peumoiae M. geitalium Mycoplasma M. peumoiae Mycoplasma M. peumoiae SDC Hif Bst µ µ p M. peumoiae M. peumoiae M. peumoiaemycoplasma Mycoplasma µ M. peumoiaem. peumoiae M. peumoiae
3 Table Straistestedithisstudy Name Strai(Type) Referece Cotetoftemplate LAMP Tt(mi.) * FH ATCC Clicalisolate 25.0 Clicalisolate 25.8 M29 ATCC Mac ATCC FH NBRC440(= ATCC553) Mycoplasmahomiis ATCC234(Typestrai) Mycoplasmasalvarium ATCC23064(Typestrai) Mycoplasmafermetas ATCC9989(Typestrai) Mycoplasmaorale ATCC2374(Typestrai) Mycoplasmageitalum ATCC33530(Typestrai) Acietobacterbaumai ATCC9606(Typestrai) Acietobacterlwofi ATCC5309(Typestrai) Alcaligeesfaecalis ATCC9209 Achromobacterxylosoxidas ATCC2706(Typestrai) Chlamydiapeumoiae KKp Chlamydiapsitaci Bud Chlamydiatrachomatis L2/434/Bu Chlamydiatrachomatis D/UW3/Cx Chryseobacterium idologees ATCC29897(Typestrai) Coxielabureti Niemile Escherichiacoli ATCC775(Typestrai) Flavobacterium breve ATCC4339(Typestrai) Flavobacterium odoratum ATCC465(Typestrai) Haemophlusifluezae ATCC9795 Klebsielapeumoiae ATCC3883(Typestrai) Legioelapeumophla ATCC3352(Typestrai) Legioelalogbeachae ATCC33462(Typestrai) Legioelabozemai ATCC3327(Typestrai) Legioelamicdadei ATCC3328(Typestrai) Moraxelacatarhalis Pseudomoasaerugiosa ATCC27853 Pseudomoasfluoresces ATCC3525(Typestrai) Pseudomoasstutzeri ATCC7588(Typestrai) Seratiamarcesces ATCC800 Staphylococcusaureus ATCC25923 Staphylococcusepidermidis ATCC4990(Typestrai) Streptococcuspeumoiae ATCC4969 Streptococcuspyogees ATCC965 * Tt(mi)deotestheamplficatiotimerequiredforthethresholdvalue(0.07)ofthevelocityofturbidityicreaseo LA320C,Loopamprealtimeturbidimetertobeexceeded. µ µ µ µ
4 M. peumoiae Fig. NucleotidesequeceofM.peumoiaeSDCrepetitiveelemet(M35024)usedtodesigtheLAMP primers.dna sequecesusedfortheprimerdesigigareshowbythesolidarrows.therestrictiosite forhifi,locatedbetweefadb,isidicatedbythedotedbox.adtheoligoucleotidesequeceof thelampprimersform.peumoiaesdcdetectioareshowithesolidliebox. p M. peumoiae Hif M. peumoiae Mycoplasma
5 Fig.2 ProtocolsforDNA extractiofrom (A)respiratoryspecimesad(B)PPLO cultures. Table2 CompositiooftheLAMP reactiomix TrisHCl KCl (NH4)2SO4 MgSO4 Twee20 Betaie dntps FIP BIP F3 B3 LoopF LoopB BstDNA polymerase Volume 20mM (ph8.8) 0mM 0mM 8mM 0.% 0.8M.4mM each 40pmol 40pmol 0pmol 0pmol 20pmol 20pmol 8Uits 20μL
6 M. peumoiae Fig.3 ComparisoofsesitivitybetweeLAMP(A),6SrRNA estedpcr(b)adpestedpcr(c)usigserialdilutiosofm.peumoiaem29geomicdna.laesadsymbols:lae/ ;600,000,Lae2/ ;60,000,Lae3/ ;6,000, Lae4/ ;600,Lae5/ ;60,Lae6/ ;6,Lae7/ ;0.6,Lae8/ ;0(copies/test),adLaeM;00bpDNA ladder, LaeM2;λHid Idigest Fig.4 SesitivityofLAMPusigserialdilutiosofplas middna icludigthetargetregiofrom them.peu moiaem29geomicdna. Symbols: ;600,000, ;60,000, ;6,000, ;600, ;60, ;6, ;0.6, ;0(copies/test) Fig.5 Agarosegelelectrophoresisadrestrictioaaly sisofthelamp productsamplifiedfrom severalm. peumoiaestrais. Lae;NBRC440 (FH), Lae2;ATCC5492 (Mac), Lae3;ATCC29342 (M29),Lae4;ATCC553 (FH), Lae5adLae6;cliicalisolates,LaeM;00bpDNA ladder, Mycoplasma
7 Table3 ResultsofdetctioforM.peumoiaefrom clicalsamplesbylampadpcr. Respiratoryspecime sputum trachealaspirate pharygealswab pleuralfluid urie trasthoraciceedleaspirate PPLO culturefrom specime sputum trachealaspirate pharygealswab pleuralfluid urie trasthoraciceedleaspirate LAMP() (%) 24(.8%) 24(.8%) LAMP() (%) 9(22.%) 9(22.%) PCR * ( ) (%) 24(.8%) 24(.8%) PCR * ( ) (%) 9(22.%) 9(22.%) * stpcrofestedpcrtargetigthe6srrna gee M. salvarium M. orale M. peumoiae M. peumoiae M. peumoiae M. peumoiae p hmw crl M. peumoiae SDC p SDC M. peumoiae M. peumoiae M. peumoiae M. geitaliummycoplasma M. peumoiae p M. peumoiae SDC SDC SDC M. peumoiae
8 M. peumoiae Mycoplasma Mycoplasma Ureaplasma Mycoplasma peumoiae Mycoplasma peumoiae M. peumoiae Mycoplasma peumoiae Chlamydia peumoiae, Legioella peumophila Mycoplasma peumoiae et al. Salmoella Mycoplasma peumoiae et al. Mycoplasma geitalium
9 Mycoplasma peumoiae Mycoplasma peumoiae Mycoplasma peumoiae Mycoplasma peumoiae et al. Mycoplasma peumoiae Mycoplasma peumoiae Mycoplasma peumoiae Mycoplasma peumoiae SDC M. peumoiae M. peumoiae
平成9年度水道事業年報 １概況 ２施設
() (mm) 12 3 31 12 3 31 4 5 6 7 8 9 10 11 12 1 2 3 145,085 146,117 146,352 146,409 146,605 146,685 146,807 147,014 147,002 147,277
QW-3414
MA1312-C P 1 2 3 A E L D E D A A E D A D D D D D E A C A C E D A A A C A C A C E E E D D D A C A C A A A A C A C A C E E C C E D D C C C E C E C C E C C C E D A C A C A C E L B B
2 7 ( ) µ 取扱説明書[NE-202]
![取扱説明書[NE-202]](/thumbs/39/20478473.jpg "取扱説明書[NE-202]")
NE-202 13.3 m m 1 2 3 m 4 5 6 7 a a a 8 9 10 11 12 a a a a 13 14 15 16 17 2.4 FH 1/XX 4 18 19 20 21 22 23 24 25 26 27 1 2 3 4 5 6 m 7 h 8 r 9 a P b c d e f g h i j ud k l m n o 28 29 30 31 32 33 34 35
1 2 http://www.japan-shop.jp/ 3 4 http://www.japan-shop.jp/ 5 6 http://www.japan-shop.jp/ 7 2,930mm 2,700 mm 2,950mm 2,930mm 2,950mm 2,700mm 2,930mm 2,950mm 2,700mm 8 http://www.japan-shop.jp/ 9 10 http://www.japan-shop.jp/
第18回海岸シンポジウム報告書
2011.6.25 2011.6.26 L1 2011.6.27 L2 2011.7.6 2011.12.7 2011.10-12 2011.9-10 2012.3.9 23 2012.4, 2013.8.30 2012.6.13 2013.9 2011.7-2011.12-2012.4 2011.12.27 2013.9 1m30 1 2 3 4 5 6 m 5.0m 2.0m -5.0m 1.0m
1 911 34/ 22 1012 2/ 20 69 3/ 22 69 1/ 22 69 3/ 22 69 1/ 22 68 3/ 22 68 1/ 3 8 D 0.0900.129mm 0.1300.179mm 0.1800.199mm 0.1000.139mm 0.1400.409mm 0.4101.199mm 0.0900.139mm 0.1400.269mm 0.2700.289mm
1 C 2 C 3 C 4 C 1 C 2 C 3 C
1 e N >. C 40 41 2 >. C 3 >.. C 26 >.. C .mm 4 C 106 e A 107 1 C 2 C 3 C 4 C 1 C 2 C 3 C 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124
平成２４年財政投融資計画ＰＤＦ出後８／０１６‐０３０
24 23 28,707,866 2,317,737 26,390,129 29,289,794 2,899,665 24 23 19,084,525 21,036,598 1952,073 24 23 8,603,613 8,393,427 967,631 925,404 202,440 179,834 217,469 219,963 66,716 64,877 3,160,423 2,951,165
液晶ディスプレイ取説TD-E432/TD-E502/TD-E552/TD-E652/TD-E432D/TD-E502D
1 2 3 4 5 6 7 1 2 3 4 5 6 7 2 2 2 1 1 2 9 10 11 12 13 14 15 16 17 1 8 2 3 4 5 6 7 1 2 3 4 5 6 7 8 9 10 9 11 12 13 13 14 15 16 17 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 1 2 3 4 5 6 7 8 9 11 12
000-.\..
1 1 1 2 3 4 5 6 7 8 9 e e 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 10mm 150mm 60mm 25mm 40mm 30mm 25 26 27 1 28 29 30 31 32 e e e e e e 33 e 34 35 35 e e e e 36 37 38 38 e e 39 e 1 40 e 41 e 42 43
EPSON LP-8900ユーザーズガイド
3 4 5 6 7 8 abc ade w p s 9 10 s s 11 p 12 p 13 14 p s 15 p s A B 16 w 17 C p 18 D E F 19 p w G H 20 A B 21 C s p D 22 E s p w 23 w w s 24 p w s 25 w 26 p p 27 w p s 28 w p 29 w p s 30 p s 31 A s B 32
2009 Aida et al. Caries Res 2006;40 2000 100 % 78.7 88.0 96.6 98.8 98.8 98.8 100.0 100.0 100 75 69.4 50 75.3 74.8 73.3 73.1 73.0 72.4 71.8 71.7 51.7 40.2 69.4 68.8 73.6 25 22.3 32.8 21.9 22.9 22.1
400 46 THE JAPANESE JOURNAL OF ANTIBIOTICS 65 6 Dec. 2012 LVFX 100 mg 3 / 7 150 mg 2 / 7 2 2006 2008 9 LVFX PK PD 2009 7 100 mg 1 3 500 mg 1 1 AUC/MIC
Dec. 2012 THE JAPANESE JOURNAL OF ANTIBIOTICS 65 6 399 45 2012 11 5 LVFX 500 mg 1 1 20 Chlamydia trachomatis C. trachomatismycoplasma genitalium M. genitalium LVFX 1 500 mg 1 1 7 22 22 C. trachomatis 17
ｎｓｇ０２－１３／ｋｙ０４５０５９３０１６０００３３２１０
φ φ φ φ κ κ α α μ μ α α μ χ et al Neurosci. Res. Trpv J Physiol μ μ α α α β in vivo β β β β β β β β in vitro β γ μ δ μδ δ δ α θ α θ α In Biomechanics at Micro- and Nanoscale Levels, Volume I W W v W
感染症学雑誌第80巻第6号
Candida Candida albicans Candida parapsilosis Candida glabrata Candida tropicalis C. albicans C. grabrata C. tropicalis µ Candida guilliermondii µc. parapsilosis µ Candida µcandida C. albicans µ µ µ µ
ネオマファーム施工標準軸組構造
注 1 注 2 注 2 SISI 1. 2. 30 35 40 50 15mm 303 (17kg/) 455 16mm, 18mm 250 (20kg/) 455 303 303 _ 6 455 650 10,465 910 2,730 1,820 1,820 910 2,275 1,365 415 450 650
untitled
-1- -2- -4- -5- 20 30 12-6- 8 15 1-7- -8- -9- -10- 40 60-11- -12- 3 4 70 90-13- -14- 2 4 3 5-15- a 3-16- -17- -18- 128 kg 150 200g 100 200g 250 750g 2 1 94 89 89 81 72 390 97 97 83 83 75 97 97 100 86
10 117 5 1 121841 4 15 12 7 27 12 6 31856 8 21 1983-2 - 321899 12 21656 2 45 9 2 131816 4 91812 11 20 1887 461971 11 3 2 161703 11 13 98 3 16201700-3 - 2 35 6 7 8 9 12 13 12 481973 12 2 571982 161703 11
0.45m1.00m 1.00m 1.00m 0.33m 0.33m 0.33m 0.45m 1.00m 2
24 11 10 24 12 10 30 1 0.45m1.00m 1.00m 1.00m 0.33m 0.33m 0.33m 0.45m 1.00m 2 23% 29% 71% 67% 6% 4% n=1525 n=1137 6% +6% -4% -2% 21% 30% 5% 35% 6% 6% 11% 40% 37% 36 172 166 371 213 226 177 54 382 704 216
+ + ø ø ø ø ø ø ø ø ø ± FH 28 D - 5S -.5 SH (5)
21.8 + + ø ø ø ø ø ø ø ø ø ++ + ++ + + + ± FH 28 D - 5S -.5 SH (5) 1 2 3 4 5 6 7 1 2 3 4 5 6 7 ** ** ** ** ** ** ** ** ** ** ** ** ** CL586-1861-4-** CL586-1868-3-** CL586-1823-5-** CL586-1835-4-** CL586-1827-6-**
感染症学雑誌第80巻第5号
Shigella Shigella sonnei S. sonnei S. sonnei Campylobacter Staphylococcus aureus S. sonnei S. boydii S. flexneri S. dysenteriae µ µµ Mse µµ Eco µ µ µ µ µ µ µmse µeco µ µ Mse Eco µ µ µ µ µeco µmse µ µ Table1
日本化学療法学会雑誌第56巻第1号
β β β β β Streptococcus pneumoniaehaemophilus influenzaemoraxella catarrhalismycoplasma pneumoniaechlamydia pneumoniae β Key wordsβ mys nilc laci ngis Table. Assessmentschedule Parameters Patientcharacteristics
LaVie Tab 安全上のご注意・サポートガイド PC-TS708T1W
PC-TS708T1W 2 3 4 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 2.4FH4 41 42 43 44 45 46 47 48 1 1 2 49 50 51 52 53 54 55 56 57 58 59 1 60 2 61
() 2
1 () 2 2 4 3 6,500 4 5 2 6 A B A B A B A B - A B 7 8 A B A B A B 9 JR JR 10 11 6 5 12 17 6 13 14 B A A B A B A B 2 1 8 15 8 16 17 9 18 3 4 5 mm mm 19 2 20 3 6 7 11 12 13 14 18 4 3 2 1 21 3 12 13 14 16
はじめに
LAMP 法を用いたウェルシュ菌エンテロトキシン遺伝子検出の検討 佐藤秀美 Development of detection of Clostridium perfringens enterotoxin gene using the LAMP assey Hidemi Sato はじめにウェルシュ菌による食中毒は, 大量に調理された食品を原因とすることが多く, しばしば大規模になるため, 原因物質の迅速な検出と判断が求められている.
l l l l 5
l l M FC00 FC00 CAC6 CAC6 SUS303.7kW SUS3kW SUS3 FC00 CAC6 SUS3 JOV FC00 CAC6 SUS0J FC00 CAC6 S5C FC00 CAC90 S5C V 00.530kW.53kW 53 l l l l 5 56 l 57 mm m l/min kw 70 l/min l/min l/min 5 7 l/min 5 l/min
DocuPrint C5450 ユーザーズガイド
1 2 3 4 5 6 7 8 1 10 1 11 1 12 1 13 1 14 1 15 1 16 17 1 1 18 1 19 1 20 1 21 1 22 1 23 1 24 1 25 1 26 27 1 1 28 1 29 1 30 1 31 1 2 12 13 3 2 10 11 4 9 8 7 6 5 34 24 23 14 15 22 21 20 16 19 18 17 2 35
limit&derivative
- - 7 )................................................................................ 5.................................. 7.. e ).......................... 9 )..........................................
2
1 12123456789012345678901234 12123456789012345678901234 12123456789012345678901234 12123456789012345678901234 12123456789012345678901234 12123456789012345678901234 12123456789012345678901234 12123456789012345678901234
a a b a b c d e R c d e A a b e a b a b c d a b c d e f a M a b f d a M b a b a M b a M b M M M R M a M b M c a M a R b A a b b a CF a b c a b a M b a b M a M b c a A b a b M b a A b a M b C a M C a M
日本化学療法学会雑誌第57巻第S-2号
Key words β I Table. Observationandtestschedule Testschedule Observation/Tests Beforetreatment Endoftreatment 5 9days aftercompletion oftreatment 4 6weeks aftercompletion oftreatment Informedconsent Patientbackground
Axygen_16p.indd
Axygen AxyPrep A Corning Brand / Axygen AxyPrep MAG Axygen IMAG MSD AxyPrep MAG 50IMAG MSD 96 15 ml 50 ml 2 Axygen AxyPrep MAG PCR AxyPrep MAG PCR PCR PCR dntps PCR AxyPrep MAG PCR Buffer 100 bp 60 bp
3 JAS JAS JAS 4
1 2 3 JAS JAS JAS 4 5 6 7 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ph 102 106 K 31 32 13 3 15 79 33 34 35 36 37 20 6 H.22.3 http://www.caa.go.jp/foods/pdf/syokuhin19.pdf 38
untitled
13 50 15 6.0 0.25 220 23 92 960 16 16 3.9 3.9 12.8 83.3 7 10 1150 90 1035 1981 1850 4700 4700 15 15 1150 1150 10 12 31 1.5 3.7%(224 ) 1.0 1.5 25.4%(1522 ) 0.7 30.6 1835 ) 0.7 1.0 40.3 (2421 ) 7.3
感染症学雑誌第77巻第8号
1 2 µ Streptococcus pneumoniae µ S. pneumoniae µ S. pneumoniae pbp β β pbp1a pbp2x pbp2b pbp mefe ermb pbp pbp1a pbp2x pbp2b pbp pbp pbp pbp 2x pbp1a pbp2x pbp 2b mefe ermb pbp β pbp2x β β β µ µ pbp3 µ
17-05 THUNDERBIRD SYBR qpcr Mix (Code No. QPS-201, QPS-201T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A4196K
17-05 THUNDERBIRD SYBR qpcr Mix (Code No. QPS-201, QPS-201T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A4196K - 18 - [1] 1 [2] 2 [3] 3 [4] 5 [5] : RNA cdna 13 [6] 14 [7] 17 LightCycler TM Idaho
M,,,,, M A A B,,,,, M M M a,,, B B, 2
, A A 1 M,,,,, M A A B,,,,, M M M a,,, B B, 2 B B B B,,, B B B B B B B M a 3 M a A A B B M, M M M M M b,,,, 4 , B B,,, A A M,,, 5 ,,,,,,,,, 6 , B B,,, 7 ,,,,,,,,,,,,,,,, 8 , 9 A B B 10 ,,,,,,, NM SHYM
OFISTAR S3100取扱説明書
2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 1 7 2 4 3 0 6 4 1 2 5 3 24 1 2 3 1 2 3 25 1 2 3 6 7 8 9 26 27 1 2 3 4 5 28 29 ABC 30 31 32 : : 33 1 8 2 1 3 1 2 3 4 34 1 2 35 1 1 36 1 2 3a 3b
1
1 2 VersionS 140mm 180mm 280mm 3,715mm 3,805mm 3,905mm 3 VersionS 4 5 VersionS VersionS 6 VersionS 7 8 VersionS VersionS 9 10 VersionS 16,500 5,350 70 2,740 2,310 540 490 5,850 8,600 4,8505,110 1,0501,050