NGS_anal_overview.v2.pptx

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4 1 st vs. 2 nd generation sequencer Sanger NGS Total bp / run 300k bp 200G bp Cost / Mb $500 $0.1 Read length 900 bp 100 bp #reads / run 384 2,000,000,000 Run time / run 8 hr 8 days Library prep labor intensive, expensive cloning needed) simple, cheap no cloning) [AB 3730XL-384well] [Illumina HiSeq2000]

5 40+)-0;;1)0#)%"+ %31-<3.3$:3K?316&1/ 6/.-+#0"&0$&-<%$=>1%< 3OJMR68?:'3./313.0=-1 >3K?316&1/ B0'00108C:&:",:'01;0.;4 + :' N Q0:36088 ) 1; N<0@@&1/ F.; N 0@@8&60=-1:@36&S6 B0'00108C:&:"",0;T0163;4!"!#$%&$')*

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12 \X:?1R'3067RM!9$ 6/.データ解析の基本戦略 それぞれのステップの 定番ソフトウェアを組 み合わせて使う,&'<&/8&-:&40% 34551/2,"+-./01/2!"#$%&' )*+$%&',"+-6-1/0& &95:&%%1;/'<471=>4=;/ 5&4?-./01/2 2&/&-4//;>4=;/ 4%%;814>&0-2&/&%?/;A7&02&-01%8;B&:C D;=E-./01/2 - 多くはUNIX向けコマ ンドラインプログラム - プログラムAからプロ グラムBに受け渡す為 のデーター変換が必要 かもしれない - 業界標準のフォーマッ トでの作業を心がける

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