Streptococcus pyogenes Streptococcus Streptococcus pyogenes Streptococcus dysgalactiae equisimilis S. pyogenes S. pyogenes S. dysgalactiae equisimilis S. pyogenes S. dysgalactiae equisimilis S. pyogenes S. dysgalactiae equisimilis Streptococcus pyogenes S. pyogenes S. pyogenes S. pyogenes S. dysgalactiae equisimilis S. pyogenes
Table Strainsevaluated Strains Lancefield sgroup Hemolysis T type source Streptococcuspyogenes(0304) Streptococcuspyogenes(03090) Streptococcuspyogenes(03) Streptococcuspyogenes(040) Streptococcuspyogenes(0404) Streptococcusagalactiae(0408) Streptococcusanginosus(003X3) Streptococcusconstelatus(SM004) Streptococcusdysgalactiaesubsp.equisimilis(003CV) Streptococcusdysgalactiaesubsp.equisimilis(003G) Streptococcusanginosus(SM003) Streptococcusconstelatus(SM004) Streptococcusdysgalactiaesubsp.equisimilis(003X) Streptococcusdysgalactiaesubsp.equisimilis(0403) B C F C G α T T T8 T4 T blood necrosis blood Streptococcus milleri S. pyogenes S. agalactiae S. anginosus S. constellatus S. dysgalactiae equisimilis S. pyogenes S. anginosus S. constellatus S. dysgalactiae equisimilis S. pyogenes µ µ S. pyogenes µ S. pyogenes S. pyogenes S. dysgalactiae equisimilis
Table Comparisonof8rapiddiagnostickitsfordetectinggroup streptococcuswithimmunochromatographicalmethod StatCheck Strep RapidTesta Strep (DaichiPureChemical (KINOSLaboratories, Co.,Ltd.) Inc.) DIPSTICK Eiken (EikenChemical Co.,Ltd.) ImmunoCard STT! (TFB,INC.) QuickVue CLEVIEW (WakoPureChemical Industries,Ltd) (NihonSchering K.K.) TESTPCK Plus (BBOTT JPN Co.,Ltd) QuickVue Dipstick Strep (SumitomoSeiyaku BiomedicalCo.,Ltd.) totalrequiredtime(min) 7 7 extractiontime(min) reactiontime(min) 4 ~ 30 (mixintheample) 30 0 8 8 ~ 30 ~ 30 ~ 30 numberofreagents 3 3 (mixintheample) packingunit(test) 0 0 0 shape platetype cardtype platetype expiraction(month) (8) 4 4 storage( ) ~30 ~ 30 ~ 30 room temperature S. pyogenes S. dysgalactiae equisimilis S. pyogenes S. anginosus S. constellatus S. pyogenes S. dysgalactiae equisimilis S. dysgalactiae equisimilis S. pyogenes
Table3 Comparisonofsensitivity(CFU/mL)of8rapiddiagnostickitsfordetectinggroup streptococcustostreptococcuspyogenesisolatedfrom clinicalsource Streptococcuspyogenes(0304)T QuickVueDipstickStrep(SumitomoSeiyakuBiomedicalCo.,Ltd.) TESTPCK Plus(BBOTT JPN Co.,Ltd) CLEVIEW (NihonScheringK.K.) QuickVue(WakoPureChemicalIndustries,Ltd) ImmunoCardSTT!(TFB,INC.) DIPSTICK Eiken (EikenChemicalCo.,Ltd.) RapidTestaStrep(DaichiPureChemicalCo.,Ltd.) StatCheckStrep(KINOSLaboratories,Inc.) Streptococcuspyogenes(03090)T QuickVueDipstickStrep(SumitomoSeiyakuBiomedicalCo.,Ltd.) TESTPCK Plus(BBOTT JPN Co.,Ltd) CLEVIEW (NihonScheringK.K.) QuickVue(WakoPureChemicalIndustries,Ltd) ImmunoCardSTT!(TFB,INC.) DIPSTICK Eiken (EikenChemicalCo.,Ltd.) RapidTestaStrep(DaichiPureChemicalCo.,Ltd.) StatCheckStrep(KINOSLaboratories,Inc.) Streptococcuspyogenes(03)T8 QuickVueDipstickStrep(SumitomoSeiyakuBiomedicalCo.,Ltd.) TESTPCK Plus(BBOTT JPN Co.,Ltd) CLEVIEW (NihonScheringK.K.) QuickVue(WakoPureChemicalIndustries,Ltd) ImmunoCardSTT!(TFB,INC.) DIPSTICK Eiken (EikenChemicalCo.,Ltd.) RapidTestaStrep(DaichiPureChemicalCo.,Ltd.) StatCheckStrep(KINOSLaboratories,Inc.) Streptococcuspyogenes(040)T4 QuickVueDipstickStrep(SumitomoSeiyakuBiomedicalCo.,Ltd.) TESTPCK Plus(BBOTT JPN Co.,Ltd) CLEVIEW (NihonScheringK.K.) QuickVue(WakoPureChemicalIndustries,Ltd) ImmunoCardSTT!(TFB,INC.) DIPSTICK Eiken (EikenChemicalCo.,Ltd.) RapidTestaStrep(DaichiPureChemicalCo.,Ltd.) StatCheckStrep(KINOSLaboratories,Inc.) Streptococcuspyogenes(0404)T QuickVueDipstickStrep(SumitomoSeiyakuBiomedicalCo.,Ltd.) TESTPCK Plus(BBOTT JPN Co.,Ltd) CLEVIEW (NihonScheringK.K.) QuickVue(WakoPureChemicalIndustries,Ltd) ImmunoCardSTT!(TFB,INC.) DIPSTICK Eiken (EikenChemicalCo.,Ltd.) RapidTestaStrep(DaichiPureChemicalCo.,Ltd.) StatCheckStrep(KINOSLaboratories,Inc.) :Strongpositive :Positive :Thetraceatthetestbandpositionwereobserved :Negative unit:cfu(colonyformingunit)/ml
Table4 ReactionoftheevalutionofhemolyticstreptococcalstrainsotherthanGroup streptococcus( CFU/mL)with8rapiddiagnostickitsfordetectinggroup streptococcus StatCheck Strep RapidTesta Strep (KINOS Laboratories, Inc.) (DaichiPure Chemical Co.,Ltd.) DIPSTICK Eiken (EikenChemicalCo.,Ltd.) ImmunoCard STT! (TFB,INC.) QuickVue CLEVIEW (WakoPure Chemical Industries,Ltd) (NihonSchering K.K.) TESTPCK Plus (BBOTT J PN Co.,Ltd) QuickVue Dipstick Strep (SumitomoSeiyaku BiomedicalCo., Ltd.) Species(StrainNo) Streptococusagalactiae(GroupBstreptococci)(0408) Streptococusanginosus(GroupCstreptococci)(003X3) Streptococusconstelatus(GroupFstreptococci)(SM004) Streptococusdysgalactiaesubsp.equisimilis(GroupCstreptococci)(003CV) Streptococusdysgalactiaesubsp.equisimilis(GroupGstreptococci)(003G) :Negative S. pyogenes S. pyogenes S. pyogenes S. pyogenes S. dysgalactiae equisimilis
Table Comparisonofsensitivityof8rapiddiagnostickitsfordetectinggroup streptococcustogroup streptococcusother thanstreptococcuspyogenesisolatedfrom clinicalsource Streptococcusanginosus(SM003) QuickVueDipstickStrep(SumitomoSeiyakuBiomedicalCo.,Ltd.) TESTPCK Plus(BBOTT JPN Co.,Ltd) CLEVIEW (NihonScheringK.K.) QuickVue(WakoPureChemicalIndustries,Ltd) ImmunoCardSTT!(TFB,INC.) DIPSTICK Eiken (EikenChemicalCo.,Ltd.) RapidTestaStrep(DaichiPureChemicalCo.,Ltd.) StatCheckStrep(KINOSLaboratories,Inc.) Streptococcusconstelatus(SM004) QuickVueDipstickStrep(SumitomoSeiyakuBiomedicalCo.,Ltd.) TESTPCK Plus(BBOTT JPN Co.,Ltd) CLEVIEW (NihonScheringK.K.) QuickVue(WakoPureChemicalIndustries,Ltd) ImmunoCardSTT!(TFB,INC.) DIPSTICK Eiken (EikenChemicalCo.,Ltd.) RapidTestaStrep(DaichiPureChemicalCo.,Ltd.) StatCheckStrep(KINOSLaboratories,Inc.) Streptococcusdysgalactiaesubsp.equisimilis(003X) QuickVueDipstickStrep(SumitomoSeiyakuBiomedicalCo.,Ltd.) TESTPCK Plus(BBOTT JPN Co.,Ltd) CLEVIEW (NihonScheringK.K.) QuickVue(WakoPureChemicalIndustries,Ltd) ImmunoCardSTT!(TFB,INC.) DIPSTICK Eiken (EikenChemicalCo.,Ltd.) RapidTestaStrep(DaichiPureChemicalCo.,Ltd.) StatCheckStrep(KINOSLaboratories,Inc.) Streptococcusdysgalactiaesubsp.equisimilis(0403) QuickVueDipstickStrep(SumitomoSeiyakuBiomedicalCo.,Ltd.) TESTPCK Plus(BBOTT JPN Co.,Ltd) CLEVIEW (NihonScheringK.K.) QuickVue(WakoPureChemicalIndustries,Ltd) ImmunoCardSTT!(TFB,INC.) DIPSTICK Eiken (EikenChemicalCo.,Ltd.) RapidTestaStrep(DaichiPureChemicalCo.,Ltd.) StatCheckStrep(KINOSLaboratories,Inc.) :Strongpositive :Positive :Thetraceatthetestbandpositionwereobserved :Negative unit:cfu/ml S. pyogenes S. dysgalactiae equisimilis S. pyogenes
S. pyogenes S. pyogenes S. pyogenes S. dysgalactiae equisimilis S. dysgalactiae equisimilis S. pyogenes S. pyogenes S. dysgalactiae equisimilis S. dysgalactiae equisimilis S. dysgalactiae equisimilis emm stg S. pyogenes S. dysgalactiae equisimilis S. pyogenes S. dysgalactiae equisimilis S. dysgalactiae equisimilis S. pyogenes S. pyogenes Streptococcus pyogenes
Streptococcus equisimilis Streptococcus dysgalactiae equisimilis Streptococcus dysgalactiae equisimilis Streptococcus dysgalactiae equisimilis et al. Streptococcus milleri
Streptococcus pyogenes Streptococcus S. pyogenes S. dysgalactiae equisimilis S. pyogenes S. dysgalactiae equisimilis S. pyogenes S. pyogenes S. dysgalactiae equisimilis