Matrix FL-JOE-TMR-CXR No.TBD015200111 DG2860 ABI PRISM 310 Genetic Analyzer ABI PRISM 377 DNA Sequencer Project ABI PRISM 310 Genetic Analyzer ABI PRISM 377 DNA Sequencer CE 4 Matrix FL-JOE-TMR-CXR 4 DNA 4 DNA TMR DNA 6- -4',5'- -2',7'- - JOE DNA - X - CXR DNA Fluorescein MatrixJOE MatrixTMR Matrix CXR Matrix Matrix FL-JOE-TMR-CXR PowerPlex 16 System (a,b) PowerPlex 1.2 System (a,b) TMR Fluorescent STR Systems (a)
STR Fluorescent STR Systems (a) Technical Manual #TMD006 PowerPlex 1.1 System (a,b) Technical Manual #TMD008PowerPlex 1.2 System Technical Manual #TMD009PowerPlex 2.1 System (a) Technical Manual #TMD011 PowerPlex 16 System (a,b) Technical Manual #TMD012 Technical Manual www.promega.com Matrix FL-JOE-TMR-CXR 20µl Fluorescein Matrix 20µl JOE Matrix 20µl TMR Matrix 20µl CXR Matrix 1ml Blue Dextran Loading Solution 20 Matrix www.promega.com DG2860 ABI PRISM 310 Genetic Analyzer VII 310 47cm 50µm 10 Genetic Analyzer Buffer Performance Optimized Polymer 4 100µS/cm POP-4 AmrescoUltra-Pure 1ml Grade 0606 100µS/cm 20 4 100µS/cm DNA 2
ABI PRISM 310 Genetic Analyzer User's Manual ABI PRISM 310 Collection ABI PRISM 310 Genetic Analyzer User's Manual GeneScan sample info GeneScan GS STR POP4 1mlA 4 30 none 2µl 25µl 953 3 48 96 Run Raw Data 1 40 GeneScan Project Raw Data sample raw data X 5 5 8 1
File New Matrix points 100,000 2 X start at Blue Fluorescein Matrix DG288A Green JOE Matrix DG284A Yellow TMR Matrix DG289A Red CXR Matrix DG290A OK GeneScan Matrix Standards System ABI GeneScan Project sample install new matrix open 4 3 400 3000rfu 3 TMR CXR PowerPlex 16 System 10 Amelogenin TMR CXR 15 Amelogenin 104 110 15 TMR CXR
ABI PRISM 377 DNA Sequencer VI TBE 10 Nalgene 0.2 Long Ranger BMA 50611 Long Ranger Singel TM pack for ABI Sequencers 36cm 377-36cmBMA 36cm 0.2mm 50691 36-10ammonium persulfate 34- V3131 TEMED V3161 Urea V3171 0.2mm Liqui-Nox ABI PRISM 377 DNA Sequencer 1 1 ABI PRISM 377 DNA Sequencer Long Ranger Blue Dextran Loading Solution TEMED Long Ranger 2 ABI PRISM 377 DNA Sequencer 36cm 1Liqui-Nox 2 0.2mm 4
2 5Long Ranger 50ml 5 Long Ranger 5 18g 6M 26ml - 10TBE 5ml 1 50Long Ranger 5ml 5 50ml Long Ranger Singel TM Packs 0.2µm Nalgene 50ml 35µl TEMED 250µl 10 30cc 2 36 34 64 96 3 2 ( ) ABI PRISM 377 Collection GeneScan Analysis Software User s Manual sample info GeneScan run none
1 2mm 377 ABI PRISM 377 DNA Sequencer User s Manual TBE 1 30cc 19 PreRun 15 20 40 Status Window 1.5µl Blue Dextran Loading Solution 1.5µl 952 3 15 20 Pause 30cc 1.5µl
Cancel 3 Run 3 GeneScan Project Raw Data sample raw data X 5 5 8 1 new points 100,000 2 X start at Blue Fluorescein Matrix DG288A Green JOE Matrix DG284A Yellow TMR Matrix DG289A Red CXR Matrix DG290A OK GeneScan Matrix Standards System ABI GeneScan Project sample install new matrix open 4 3 400 3000rfu 3
TMR Matrix Raw Data TMR Matrix ABI PRISM 310 Genetic Analyzer GeneScan Analysis raw data sample start at 3529 2 1Liqui-Nox 2N HCl 15 PowerPlex 16 System 2 1,000rfu VI PowerPlex 16 System Technical Manual, #TMD012 IX.A
Project run GeneScan New Project Project Add Sample Files GeneScan 2 2 3 3 0.3233 2 3 0.6269 2 0.02 3Save As 6 8 3 4.06.08.0 10.0 2 4 2 0.3233 0.6269 Sample Install New Matrix 2 V.A 2 1 3
4 B 4 CD B C 1 2 3 4 4 A B C B
E-Mail: prometec @jp.promega.com CE ABI PRISM 310 Genetic Analyzer 100µS/cm 20 4003000rfu 5 Fluorescein: Blue JOE: Green TMR: Yellow CXR: Red CE 1 1 2 CE ABI PRISM 310 Genetic Analyzer 10Genetic Analyzer Buffer
CE 1 ABI PRISM 310 Genetic Analyzer 5 III.B 1 IV.D 1 V start at start at 6
5 ABI PRISM 310 Genetic Analyzer GeneScan Analysis 270 6 Matrix FL-JOE-TMR-CXR 4 ABI PRISM 310 Genetic Analyzer GeneScan Analysis start at JOE Matrix 4
10 Blue Dextran 0.05g 88.25 500µl 15mg/ml blue dextran 4.1mM EDTA (ph 8.0) 50ml 250µl 10 TBE 10 107.8g Tris base 7.44g EDTA Ultra-Pure Grade (Na 2 EDTA2H 2 O) 55.0g Amresco 0606 Tris base EDTA 800ml ph 100µS/ ph 8.3 cm 1 (a)str loci are the subject of U.S. Pat. No. 5,766,847 and German Pat. No. DE 38 34 636 C2, issued to the Max-Planck-Gesellschaft zur Forderung der Wissenschaften, ev, Germany. Exclusive rights have been licensed to Promega Corporation for uses in human clinical research and diagnostics applications and all forms of human genetic identity. The development and use of STR loci is covered by U.S. Pat. No. 5,364,759 assigned to Baylor College of Medicine, Houston, Texas. Rights have been licensed to Promega Corporation for all applications. U.S. Pat. No. 5,599,666 has been issued to Promega Corporation for allelic ladders for the loci CSF1PO, F13A01, FESFPS, LPL and vwa. U.S. Pat. No. 5,674,686 has been issued to Promega Corporation for allelic ladders for the locus CSF1PO and the combination of allelic ladders for the loci CSF1PO, FESFPS and TH01. U.S. Pat. No. 5,783,406 has been issued to Promega Corporation for allelic ladders for the locus CSF1PO. U.S. Pat. No. 6,156,512 has been issued to Promega Corporation for allelic ladders for the loci D16S539, D7S820, D13S317 and D5S818. Use of Promega s STR Systems requires performance of the polymerase chain reaction (PCR), which is the subject of European Pat. Nos. 201,184 and 200,362, and U.S. Pat. Nos. 4,683,195, 4,965,188 and 4,683,202 owned by Hoffmann-La Roche. Purchase of Promega s STR Systems does not include or provide a license with respect to these patents or any other PCR-related patent owned by Hoffmann-La Roche or others. Users of Promega s STR Systems may, therefore, be required to obtain a patent license, depending on the country in which the system is used. For more specific information on obtaining a PCR license, please contact Hoffmann-La Roche. (b)u.s. Pat. No. 5,843,660 has been issued to Promega Corporation for multiplex amplification of STR loci. Other patents are pending. 2000, 2001 Promega Corporation. All Rights Reserved. GenePrint and PowerPlex are trademarks of Promega Corporation and are registered with the U.S. Patent and Trademark Office. ABI PRISM and GeneScan are registered trademarks of The Perkin-Elmer Corporation. Liqui-Nox is a registered trademark of Alconox, Inc. Long Ranger is a registered trademark of FMC Corporation. Long Ranger Singel is a trademark of BMA, Inc. Nalgene is a registered trademark of Nalge Nunc International. All prices and specifications are subject to change without prior notice. Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date information on Promega products.