NanoVue 71-2888-36
... 2... 2... 2... 3... 4... 5... 6 NanoVue...6 NanoVue Sample... 7 Life Science... 9 1. DNA/RNA... 10 2. CyDye... 12 3.... 15 3-1Protein UV UV Protein A280 A280... 15 3-2BCABradford LowryBiuret... 20 4. Utilities... 25 4-1... 26 4-2... 26 4-3... 26 4-4... 26 4-5...27 4-6...27 4-7NanoVue... 28 5.... 29 5-1... 29 5-2... 30 5-3... 30 5-4... 30... 31 1. PRINT VIA COMPUTER...31 2....31 PVC 5.2.2.2E v1.5... 32 PVC 5.1.0.1E v1.4... 34 Print Via Computer PVC... 36 Print Via Computer PVC... 37 1
5 35 1 4 2 3 31 80% 31 40 50% 4.5 kg AC 90 240 V 50 60 Hz 2 3 20 2
4 English 2 abc 2 ABC C 1 1 _ 1 _ 1 3
1 0A/100%T : NanoVue : : 1. 2. 3,4,5,6 7. 8. 9. Utilities p.26 4
1 5. Utility 2. Regional Language Japanese 2 5. Utility4. Preferences Quality Assurance On 2 2 Note: 1 Off On Quality Assurance On Quality Assurance On 2 3 5 µl 2 µl 1 2 5
NanoVue 1 2 3 9 4 9 9 5 6
NanoVue sample 1 2 2 µl 1 3 1 Note >Lid Switch 8 5 4 5 >Quality Assurance 7 2 2 2 6 23 3 7 2 µl 8 >Lid Switch 10 9 10 4,5 Auto-print USB Bluetooth 11 6 7
12 7 10 13 **. Lid Switch > **. Lid Switch Auto-Read Utility Preferences Lid Switch On / Methods Favorites / p.26 Utility RegionalJapanese English Utility Printer Utility Printer Auto-Print On Printer Built-in USB Computer<USB> Quality Assurance Utility Preferences Quality Assurance On 2 2 8
Life Science 6 DNA 1 DNA RNA 2 RNA Oligo 3 Tm Calculation 4 DNA Tm CyDye 5 cdna Protein 6 Life Science 10 mm 9
1. DNA/RNA 230 nm260 nm280 nm320 nm 260 nm 280 nm Abs260/Abs2801.8 260 nm 230 nm Abs260/Abs230 EDTAGTC 2.0 DNA 50RNA 40 220 320 nm 1 DNA 1 RNA 2 2 0.2 mm0.5 mm AutomaticAuto 0.5 mm 0.2 mm AutomaticAuto 3 1.00 9999 C 3 2 Volume Diluent 0.019999 1 µl 99 µl Volume 1 Diluent 99 Dilution Factor 100 1.00 9999 4 320 nm 5 µg mlng µlµg µl µg ml Factor 10
6 DNA 50RNA 40 0.01 9999 7 8 9 230 nm260 nm280 nm 320 nm 2 Abs260/Abs280Abs260/Abs230 260 nm 9 DNA RNA 1.p.9 1 2. 3. 220 320 nm 230 nm260 nm280 nm 320 nm 7. Sample Number: 1 8. Save Method: 9.Auto-Print: 11
2. CyDye DNA 1 CyDye 2 3 Cy3...550 nm Cy5...649 nm Fluorescein...495 nm 4 Coefficient µl mol-1cm-1 5 0.2 mm0.5 mm AutomaticAuto 0.5mm 0.2 mm AutomaticAuto 6 8 7 8 Dilution Factor... Factor...DNA DNA RNA 40 33 Volume... µl Start RNA... RNA ng Background... Cy3 Cy5 430 nmfluorescein 400 nm 12
9 10 11 230 nm260 nm280 nm 3 A260/A280A260/ A230Adye/A260 260 nm DNA 4 Total DNA... cdna ng DNA Yield...Start RNA cdna Total cdna/start RNA 100 % Nucleotides... Nucleotides Dye Per Probe... pmol/ul 13
1. 2. 3. / 4. CyDye 7. Sample Number: 1 8.Save Method: 9.Auto-Print: / CyDye 14
3. 5 7 Protein Protein UV UV BCABradford LowryBiuret 5 UVChristian Warburg A 280280 nm BSAIgG BCA 3-1Protein UV UV Protein A 280 A 280 280 nm 260 nm Christian Warburg Biochemische Zeitung 310, 384 1941 mg ml=1.55 Abs 280-0.76 Abs 260 1 = 1 Abs 280-2 Abs 260 320 nm Abs 260 Abs 280 2 2 Abs 260 2 0 280 nm 2 0.00 1 BSA 1=1.115 0 0.8 mg ml mg ml=1.115 Abs 280 15
Protein UV 1 1 Protein UV UV 2 0.2 mm0.5 mm AutomaticAuto 0.5 mm 0.2 mm AutomaticAuto 3 1.00 9999 C 3 2 Volume Diluent 0.01 9999 1 µl 99 µl Volume 1 Diluent 99 Dilution Factor 100 1.00 9999 4 320 nm 5 260 nm 1 0.76 0 9999 6 280 nm 1 1.55 0 9999 7 µg mlng µlµg µl 16
8 9 10 260 nm280 nm 320 nm 10 Protein UV 1.p.12 1 2. 3. 230 330 nm 230 nm260 nm280 nm 320 nm 7. Sample Number: 1 8. Save Method: 9.Auto-Print: 17
Protein A 280 1 1 Protein UV UV 2 0.2 mm0.5 mm AutomaticAuto 0.5 mm 0.2 mm AutomaticAuto 3 1.00 9999 C 3 2 Volume Diluent 0.01 9999 1 µl 99 µl Volume 1 Diluent 99 Dilution Factor 100 1.00 9999 4 320 nm 5 Christian WarburgBSAIgGLysozymeMolarextinction Mass ExtinctionE 1% Christian WarburgWarburg Christian mg/ml= 1.55 Abs 280 Abs 260 BSA10 mg/ml BSA mg/ml= 1.115 Abs 280 IgG10 mg/ml IgG Lysozyme10 mg/ml Lysozyme 18
Molar extinction M -1 cm -1 Mass Extinction L g -1 cm -1 E 1%10 mg/ml1 % L g -1 cm -1 6 7 8 260 nm280 nm 320 nm 8 Protein UV 1.p.18 1 2. 3. 230 330 nm 230 nm260 nm280 nm 320 nm 7. Sample Number: 1 8. Save Method: 9.Auto-Print: 19
2-2BCABradford Lowry Biuret 2-1 Protein UV UV 4 BCA 2 1 BCA 562 nm Bradford 595 nm BSA Lowry Folin-Ciocalteu phenol 750 nm BSA Biuret 2 546 nm 4 BSA NanoVue BCA 0.5 20 µg/ml 1 2000 µg/ml Bradford 125 1500 µg/ml 10 100 µg/ml Lowry 10 1500 µg/ml 5 100 µg/ml Biuret 1 15 mg/ml 130 mg/ml 1 BCA 2 Bradford 3 Lowry 4 Biuret 5 2 BCA 562 nm Bradford 595 nm Lowry 750 nm Biuret 546 nm 3 3 4 1 9 0 20
4 8 µg/mlµg/µlpmol/µlmg/dlmmol/lµmol/lg/lmg/lµg/l U/I%ppmppbconc DP 0 2 DP 5 DP 1 98768.2 98768 3 5 Regression, Zero Regression, Interpolation, 3 Cubic Spline, 6 Standards Manual Standards p.23 7-1 15-1 Manual p.23 p.24 7-2 12-2 6 Standards 7-1 Replicates Off1 2 3 8-1 Standards Protein Standards 9-1 0.001 9999 C Std.1 10-1 Calibration 2 Parameters 21
Calibration 11-1 Replicates 12-1 C C Replicates 13-1 OK Standards Next C 22
14-1 15-1 15-1 Proein 1.p.16 1 2. 3. 7. Sample Number: 1 8. Save Method: 9.Auto-Print: 6 Manual Standards 7-2 0.001 9999 C 8-2 Calibration 2 Parameters 23
Calibration 9-2 0.001 9999 C 10-2 Standards 11-2 12-2 12-2 Protein 1.p.20 1 2. 3. 7. Sample Number: 1 8. Save Method: 9.Auto-Print: 24
4. Utilities 5 Utilities 1 2 3 4 5 6 7 8 25
4-1 Day Month Year Hour Minute Utilities 4-2,. Utilities 4-3 Built in USB Bluetooth Utilities 4-4 Games: Games Yes No Theme: History: Auto Standby: 1 2 Auto-Print: Auto-Print On 26
Quality Assurance: 2 Quality Assurance On Utilities 4-5 Utilities 4-6 Utilities 27
4-7NanoVue 1 :NanoVue Calibration Fluid 28-9244-05 1 Utility 7 Pathlength Calibration 2 Certificate ID NanoVue Calibration Fluid 3 Calibration Value NanoVue Calibration Fluid Cal 4 OK 5 5 µl 2 1 2 6 NanoVue Calibration Fluid 1 2% Status OK OK (C) 7 10 OK OK Utility 8 0.5mm 0.2mm 10 / 0.5mm 0.05mm 0.2mm 0.02mm 2.0% Utility 28
5. 4-1 Pathlength Calibration NanoVue Calibration Fluid Calibration Value 1 NanoVue [C] Path factor disabled 2 Applications2 Single Wavelength1 259nm 0.5mm 0.5 mm 0.2 mm 3 NanoVue Calibration Fluid Calibration Value 1/2 0.020 5 4 0.5mm 1 0.07mm Calibration Value 1/2 0.020 5 Single Wavelength1 259nm 0.2mm 6 0.2mm 1 0.07mm Calibration Value 1/5 0.015 7 [C] Path factor disabled 8 9 29
4-2 1 2 3 4 5 4-3. 4-4 1 2 3 Spare Paper for Printer 20 rolls 28-9182-26 30
1. PRINT VIA COMPUTER PRINT VIA COMPUTER PVC5.2.2.2 v1.5 32 33 PVC5.1.0.1 v1.4 34 35 PVC v1.0 1.1 36 CD-ROM PDF TEL: 03-5331-9336 2. CD- ROM PDF CD-ROM GE TEL:03-5331-9336 FAX:03-5331-9370 E-mail:Tech-JP@ge.com 31
PVC 5.2.2.2.E v1.5 USB USB USB ver2.0 OS Windows2000 Windows XP sp2 OS Administrator User / Guest PVC PC FTDI USB Serial Converter Drivers PVC CD-ROM Microsoft.NET Framework PC PVC Software FIDI USB Serial Converter Drivers USB Serial Port USB USB PC 32
1. CD-ROM PC PVC 2. setup.exe 3. ReadMe / 4. USB 5. PVC Software C:\Program Files\PVC\ Next 6. USB USB USB 6 7. Installation Complete Close 8. PC USB PC PC 9. USB to Serial ConverterUSB <-> Serial 10. USB Serial Port 9 11. PC PVC PVC PVC Application 12. PVC Application file > Setup Com Port Printer Tools 13. PVC Application Option / / Microsoft ExcelTM, Microsoft WordTM 14. 37 PVC 33
PVC 5.1.0.1.E v1.4 USB USB USB ver2.0 OS Windows2000 Windows XP sp2 OS Administrator User / Guest PVC PC FTDI USB Serial Converter Drivers PVC CD-ROM Microsoft.NET Framework PC PVC Software USB FIDI USB Serial Converter Drivers USB Serial Port USB USB PC 34
1. CD-ROM PC PVC 2. setup.exe 3. PC Microsoft.Net Framework 4 4. PVC Software C:\Program Files\PVC\ Next 5. PC USB PC PC 6. USB to Serial ConverterUSB <-> Serial 7. USB Serial Port 6 8. PC PVC PVC PVC Application 9. PVC Application file > Setup Com Port Printer Tools 10. PVC Application Option / / Microsoft ExcelTM, Microsoft WordTM 11. 37 PVC 35
Print Via Computer PVC v1.0 v1.1 Print Via Computer PVC NanoVue PCPC PVC PC PVC Viewer PVC PC OS Windows 2000 TM Windows XP TM OS 1. PC CD-ROM PC PC NanoVue 2. CD-ROM PC 3. PC 10 4. OK Yes PC 5. PC 6. NanoVue PC USB 7. PC The Found New Hardware Wizard PVC 6 7 8. PC NanoVue PC Found New Hardware PVC 8 36
PVC PC 1. NanoVue Utilities Auto-Print Printer USB 2. PC PVC 3. PVC COM COM 4. PVC PC NanoVue 1 Connected PC PVC Running 5. PVC 16 18 Setup 6. PVC 1 option 2 PVC 19~20 7. PC PC Wave Scan NanoVue PC 8. print 9. PC PVC PC PC file Setup 10. PVC Viewer 37
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