UM PM Q24 ( /2013)
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- ありみち うづき
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1 July 2011 PyroMark Q24 Sample & Assay Technologies
2 1 User Manual 1 Safety Information 2 User Manual 2 Introduction 3 User Manual 3 General Description PyroMark Q External Application PyroMark Q24 Software PyroMark Q24 Vacuum Workstation DNA PCR 8 2 PyroMark Q24 07/2011
3 5.3.4 DNA PyroMark Q24 Plate PyroMark Gold Q24 Reagents PyroMark Q Waste Container PyroMark Q PyroMark Q PyroMark Q24 Vacuum Workstation PyroMark Q24 Vacuum Workstation Vacuum Workstation PyroMark Q24 07/2011 3
4 4 4.1 PyroMark Q24 QIAGEN CD-ROM PDF PyroMark Q24 User Manual CD-ROM PDF PyroMark Q24 Software User Guide USB PyroMark Q24 Vacuum Workstation PyroMark Q24 Plate Holder 4.2 PyroMark Q24 PyroMark Q24 Vacuum Workstation User Manual Appendix A PyroMark Q24 PyroMark Q24 Vacuum Workstation User Manual Appendix A PyroMark Q24 70 cm 27 inch 10 cm 4 inch PyroMark Q m 59 inch PyroMark Q24 PyroMark Q24 10 cm PyroMark Q24 PyroMark Q V AC Hz 160 VA PyroMark Q24 Vacuum Workstation 100 V AC 50/60 Hz 25 VA PyroMark Q24 10 PyroMark Q24 3 AC 4 PyroMark Q24 07/2011
5 5 PyroMark Q24 User Manual 3.4 PyroMark Q24 Instrument PyroMark Q Administration 2. Set Date and Time OK Set USB 1. USB USB 2. Administration OK 3. Copy Unsaved Runs OK 4. Select 5. USB Close 6. USB USB 1. USB USB 2. Administration OK 3. Copy Recently Saved Runs OK 4. Select 5. USB Close 6. USB PyroMark Q24 07/2011 5
6 5.1.4 QIAGEN 1. USB USB 2. Administration OK 3. Copy Log Files OK 4. USB Close 5. USB USB USB 2. Administration OK 3. Extract Damaged Runs OK 4. USB Close 5. USB PyroMark Q24 PyroMark Q24 Software PC *.pyrorun *.pyrosetup PyroMark Q24 *.pyrorun *.pyrosetup About OK 2. OK QIAGEN 1. USB USB upgrade 2. USB USB 3. Administration OK 4. Upgrade Software OK External Application Run External Application QIAGEN 6 PyroMark Q24 07/2011
7 5.2 User Manual 1 Safety Information PyroMark Q24 Software User Guide PyroMark Q24 Software Windows StartAll Programs / PyroMark / PyroMark Q24 F1 5.3 PyroMark Q PyroMark Q24 Vacuum Workstation 80 PyroMark Q24 Plate cat. no PCR PyroMark Q24 Cartridge cat. no Streptavidin Sepharose High Performance 34 µm 5 ml GE Healthcare Milli-Q 70 PyroMark Binding Buffer cat. no PyroMark Denaturation Solution cat. no PyroMark Wash Buffer concentrate cat. no PyroMark Annealing Buffer cat. no PyroMark Q24 Vacuum Workstation PyroMark Q24 Vacuum Workstation 1. PCR 100 µl ml Vacuum Prep Tool Waste ContainerWaste Container 6. PCR PyroMark Q24 07/2011 7
8 5.3.2 DNA 5' PCR DNA User Manual Appendix B PCR PCR Streptavidin Sepharose High Performance, GE Healthcare PCR 39 µl µl Binding buffer 40 µl 80 µl PCR Streptavidin Sepharose HP Lot. no Lot. no. 1 µl Lot. no. 2 µl 2 µl PCR 38 µl PyroMark PCR Kit PCR 5 10 µl 1 PCR 1 40RLU Streptavidin Sepharose HP 1 µl PyroMark Binding Buffer 40 µl PCR 5 20 µl 80 µl 4. PCR 5. PCR ,400 rpm Vacuum Workstation DNA PyroMark Q24 Plate Vacuum Workstation Denaturation Solution MSDSs OSHA*/ACGIH /COSHH QIAGEN * OSHA Occupational Safety and Health Administration (United States of America). ACGIH American Conference of Government Industrial Hygienists (United States of America). COSHH Control of Substances Hazardous to Health (United Kingdom). 8 PyroMark Q24 07/2011
9 5.3.5 PyroMark Q24 Plate Holder PyroMark Q24 Vacuum Workstation Waste Conteiner PyroMark Q24 Vacuum Workstation 5 50 ml ml Denaturation Solution 2 50 ml 1 x Wash Buffer 3 50 ml 4 70 ml 5 PCR Plate Q24 Plate 4 5 P PyroMark Q24 07/2011 9
10 3. 4. On Vacuum Prep Tool Tool ml Waste Container Tool Tool Off Parking P ml 5 8. Annealing Buffer 0.3 µm 25 µl PyroMark Q24 Plate Holder 9. PCR PyroMark Q24 Plate 10. On Tool 11. PCR 15 Tool Tool Tool Denaturation Solution Tool Washing Buffer Tool Tool PyroMark Q24 Plate Tool Off Tool Off Off 10 PyroMark Q24 07/2011
11 18. PyroMark Q24 Plate Vacuum Prep Tool 19. Tool 4 Off On 5 70 ml 21. Tool Tool Off Tool Parking P PyroMark Q24 Vacuum Workstation Plate Holder F 1. PyroMark Q24 Plate Holder Vacuum Workstation PyroMark Q24 Plate PyroMark Gold Q24 Reagents 1. PyroMark Gold Q24 Reagents EnzymeSubstrate 620 µl dntp 2 8 dntp S Substarate E Enzyme A.C.G.T dntps PyroMark Q24 07/
12 3. ToolsPre Run Information PyroMark Q24 PyroMark Q24 PyroMark Q24 PyroMark Q24 QIAGEN Warning Messages 7 hh:mm 12 PyroMark Q24 07/2011
13 PyroMark Q24 Plate USB USB USB USB 2. Run OK 3. SelectBack 4. Select PyroMark Q24 07/
14 5.5.4 Environment Priming Running Stopped Saving Finished USB USB USB Warning Messages Warning Messages 3 7 Stop USB Close 2. USB PyroMark Q24 Plate PyroMark Q24 Plate PyroMark Q24 07/2011
15 Milli-Q PyroMark Q24 07/
16 Shutdown OK 2. It is now safe to turn off the instrument Waste Container Vacuum Workstation Denaturation Solution MSDSs OSHA*/ACGIH /COSHH QIAGEN * OSHA Occupational Safety and Health Administration (United States of America). ACGIH American Conference of Government Industrial Hygienists (United States of America). COSHH Control of Substances Hazardous to Health (United Kingdom). Milli-Q 1. Vacuum Prep Tool Waste Container 5. Vacuum Workstation PyroMark Q24 07/2011
17 Light button 1/3 1/4 QIAGEN Tel PyroMark Q24 07/
18 6 PyroMark Q24 User Manual 1 Safety Information PyroMark Q24 PyroMark Q24 1 QIAGEN Warranty ExtensionFull Cover Agreement QIAGEN Tel techservice-jp@qiagen.com 6.1 PyroMark Q24 PyroMark Q24 Validation Oligo cat. no AQ CpG PyroMark Q24 PyroMark Q24 Validation Oligo QIAGEN Tel techservice-jp@qiagen.com 6.2 PyroMark Q Shutdown OK 2. It is now safe to turn off the instrument 18 PyroMark Q24 07/2011
19 dispensing unitprocess chamber Heating block 70 User Manual 3 General Description 3.4 PyroMark Q24 Instrument PyroMark Q Shutdown OK 2. It is now safe to turn off the instrument PyroMark Q24 07/
20 6.3 PyroMark Q24 Vacuum Workstation Vacuum Workstation Denaturation Solution MSDSs OSHA*/ACGIH /COSHH QIAGEN * OSHA Occupational Safety and Health Administration (United States of America). ACGIH American Conference of Government Industrial Hygienists (United States of America). COSHH Control of Substances Hazardous to Health (United Kingdom) PyroMark Q24 Vacuum Workstation Vacuum Workstation Milli-Q 1. Vacuum Prep Tool Tool 4. Tool PyroMark Q24 07/2011
21 mm Allen key Milli-Q PyroMark Vacuum Prep Filter Probe cat. no Vacuum Prep Tool Tool 4. 2 mm Allen key PyroMark Q24 07/
22 mm Allen key Seal - VPT Filter probes, PyroQ24VW cat. no Vacuum Prep Tool Tool 4. 2 mm Allen key Tool PyroMark Q24 07/2011
23 6.3.4 Waste Container Cap-Vacuum bottle, PyroVW Cat. no Vacuum Prep Tool Vacuum 100 cm Vacuum Tube 50 cm Vacuum Tube 6. PyroMark Q24 07/
24 6.3.5 Waste Container 2 Vacuum Workstation Millipore Millex-FG50 Filter Unit Catalogue Number SLFG Vacuum Prep Tool Waste Container PyroMark Q24 07/2011
25 7 QIAGEN QIAGEN QIAGEN QIAGEN 1. Run Information PyroMark Control Oligo QIAGEN PyroMark Q24 07/
26 7.1 a DNA PCR b PCR c 5 PCR d e f PCR g h Substrate PCR 1 DNA PCR PCR 1 PCR User Manual Appendix B HPLC User Manual Appendix B PCR 5.3 QIAGEN 26 PyroMark Q24 07/2011
27 a b c d Substrate e Pre Run Information ToolsPre Run Information Tools Pre Run Information f PyroMark Gold Q24 Reagents Handbook 20 Annealing Buffer g a SNP b PCR dutp c d e f g SNP dutp dutp dttp Dispensation Order QIAGEN PyroMark Q24 07/
28 7.2 PyroMark Q24 Analysis Software User Guide Troubleshooting 7.3 a Too many unsaved runs in the USB instrument. Please go to folder Unsaved Runs and save them to USB stick b The required value was not reached. The run will be stopped c Run name is invalid. d Could not copy file to USB stick e Failed to connect to the mixer, please restart the instrument Restart f Failed to connect to the hardware, please restart the instrument g Connection to the hardware is lost, please restart the instrument h No valid upgrade folders/files found on USB stick QIAGEN Tel techservice-jp@qiagen.com PyroMark Q24 Software USB QIAGEN USB QIAGEN QIAGEN QIAGEN USB upgrade User Manual Troubleshooting QIAGEN a b USB USB c USB USB QIAGEN 28 PyroMark Q24 07/2011
29 7.4 Vacuum Workstation a b c Waste Container Waste Container Vacuum Workstation a b c d PyroMark Q24 Plate PCR PyroMark Q24 Plate 7.5 PyroMark Q24 PyroMark Control Oligo Cat. no PyroMark Control Oligo PyroMark Q24 PyroMark Q24 Vacuum Workstation QIAGEN Tel techservice-jp@qiagen.com PyroMark Q24 07/
30 30 PyroMark Q24 07/2011
31 PyroMark Q24 07/
32 Trademarks: QIAGEN, PyroMark (QIAGEN Group). Trademarks and Disclaimers QIAGEN Handbook User Manual QIAGEN, all rights reserved Forefront Tower II Tel: Fax: /2013 Sample & Assay Technologies
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