The Next Stage Mastercycler X50

Eppendorf Mastercycler X50 3»We cannot solve our problems with the same thinking we used when we created them.«albert Einstein, Physicist Mastercycler X50 Gradient Eppendorf PCR 2Dgradient Steady- Slope * 2 * PCR 10 C/s PCR > 6 7 > 8 > 9

4 Eppendorf Mastercycler X50 The Next Stage of Engineering 2D-Gradient Mastercycler X50 flexlid 1 10 50 > PCR 2D-Gradient > 10 C/s > 384 > > 10 > flexlid > > 2 1 10 PC 50 2D-Gradient 2 Application Note 387 > Fast PCR > PCR > PCR > >

Eppendorf Mastercycler X50 5 10 C/s Application Note 274 Mastercycler X50 Mastercycler X50 > > 詳しくはこちら : www.eppendorf.com /mastercycler

6 Eppendorf Mastercycler X50 The Next Stage of PCR Optimization PCR PCR GC-rich DNA DNA Eppendorf 2D-Gradient Cross-Examination T A 51.9 52.3 53.5 55.4 58.2 60.8 63.2 65.9 68.5 70.5 71.7 72.3 T D 99 2D-Gradient -actin PCR 35 PCR -actin 98.5 97.3 95.6 12 51.9 ~72.3 C 90.2~99 C 8 65.9 C 70.5 C 93.4 93.4 C 95.6 C 2 95 C 91.7 90.6 90.2 > > 詳しくは Application Note 387をご覧ください

Eppendorf Mastercycler X50 7

8 Eppendorf Mastercycler X50 The Next Stage of Speed PCR 10 C/s 5 C/s Mastercycler X50 [hh:mm:ss] [ C/s] Mastercycler X50s* 00:39:29 10 Mastercycler X50l* 00:45:02 5 TAdvanced 96S 00:47:05 8 PeqSTAR 96X 00:47:10 5 TAdvanced 96 00:47:37 6 Biorad C1000 00:49:18 5 Agilent SureCycler 8800 00:50:33 6 Proflex (96-well) 00:50:54 6 Mastercycler nexus gradient* 00:51:15 3 Applied Biosystems Veriti Fast 00:56:13 5 SimpliAmp 00:56:44 4 Biorad T100 01:03:52 4 * Eppendorf > > 詳しくは Application Note 274をご覧ください :»Comparative run time evaluations of PCR thermal cyclers«.

Eppendorf Mastercycler X50 9 PCR PCR PCR Mastercycler X50 > 0.2 C at 20-72 C > 0.15 C > > > > >

10 Eppendorf Mastercycler X50 Mastercycler X50s Mastercycler X50a Mastercycler X50p Mastercycler X50h *1 96 384 0.1/0.2 ml 4 99 C Fast, Intermediate, Standard, Safe 6 2D-Gradient 12 8 24 16 1 30 C 30 99 C 37 110 C flexlid *2 20 C 72 C 95 C ±0.2 C ±0.3 C ± 0.15 C 10 C/s 5 C/s 5 C/s 2.3 C/s USB (W D H) 27.5 43 33 cm 11.5 kg 100 V, 50 60 Hz 850 W *1 *2

Eppendorf Mastercycler X50 11 Mastercycler X50i Mastercycler X50l Mastercycler X50r Mastercycler X50t 4 99 C Fast, Intermediate, Standard, Safe 6 12 8 24 16 1 30 C 30 99 C 37 110 C flexlid *2 ±0.2 C ±0.3 C ± 0.15 C 10 C/s 5 C/s 5 C/s 2.3 C/s USB 27.5 43 33 cm 10.7 kg 100 V, 50 60 Hz 850 W

Order no. Mastercycler X50s 96 6311 000.010 1,200,000 Mastercycler X50a 96 6313 000.018 1,130,000 Mastercycler X50p 96 6315 000.015 1,200,000 Mastercycler X50h 384 6316 000.019 1,200,000 Mastercycler X50i* 96 6301 000.012 990,000 Mastercycler X50l* 96 6303 000.010 920,000 Mastercycler X50r* 96 6305 000.017 990,000 Mastercycler X50t* 384 6306 000.010 1,060,000, 5 m 6313 070.040 * Mastercycler X50 s a p h 9 www.eppendorf.com/mastercycler 101-0031 2-4-5 Tel: 03-5825-2361 Fax: 03-5825-2365 Email: info@eppendorf.jp BIO-RAD C1000 is a registered trademark of Bio-Rad Laboratories, Inc., USA. Agilent SureCycler 8800 is a registered trademark of Agilent Technologies, Inc., USA. Proflex is a registered trademark of Life Technologies Corp., USA. Applied Biosystems Veriti Fast is registered trademark of Applied Biosystems LLC, USA. SimpliAmp is a registered trademark of Life Technologies Corp., USA. Eppendorf, the Eppendorf Brand Design, Mastercycler, SteadySlope and flexlid are trademarks of Eppendorf AG, Germany. All rights reserved, including images and graphics. Copyright 2017 by Eppendorf AG. 25C0817S Printed in Japan.