1200 50 1000 Growth (mv) 400 45 40 35 Temperature (!"! 200 30 0 25 0 10 20 30 40 50 Time (hr) CO 2
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, Zymomonas
Z. mobilis A. pasteurianus SKU1108 Acidity (%) 4 3 2 1 0 38.5 C Wild 37 ºC 80-times 7 times 344 hrs Adaptation at 40! 39 ºC 50-times 39 C 40 ºC 70-times 80M 130M 200M 10 times 458 hrs 39.5 C 1 times 24 hrs 0 500 1000 1500 2000 Cultivation time (Hr) EtOH % (w/v) $! #! )**+% "$%&# "! '%&# Wild (!'#!!!! "#! #%! $&! %'!!"#$%&'(% 40 C!"(#)*+,-./# 21 times 1700 hrs TH-3 strain Zymomomas
IFO ) Acetobacter IFO pasteurianus Gluconacetobacter xylinus Gluconobacter frateurii SKU1108 IFO IFO CHM43 39 C ~37 C ~33 C ~30 C ~38.5 C 35 C 37 C 42 C 40 C 41 C (TH-3) 35 C 39 C (43AD) Tanticharoenia sakaeratensis NBRC ~34 C (35 C)
Kluyveromyces marxianus Zymomonas mobilis NBRC ~39 C 45 C 45 C 45 C CP4 ~38 C 40 C TISTR ~39 C 41 C (200M) Corynebacterium glutamicum KY9002 ~38 C 40 C 40 C 42 C
A. pasteurianus IFO ) A. pasteurianus IFO A. pasteurianus SKU1108 Ga. xylinus IFO 30 C 40 C: 40C(5) (2) 42 C: 42C (4) 42 C: 42CS (15) 37 C 38 C: NM-2 (4) 38.5 C: NM-3 (7) 39 C: NM-4 (8) 39.5 C: NM-5 (11) 40 C: NM-6 (13) 38.5 C 39 C: TH-1 (4) 39.5 C 40 C: TH-3 (11) 34 C: ITO-1 (3) 34.5 C: ITO-2 (6) 35 C: ITO-3 (8) 11; 92-kbp1; (pl) 3 8; 1; (Tn) 1; (64-kbp) 1; 1; pl 1 7; Tn 2; 2 4; 2; 2 G. frateurii CHM43 38.5 C: CHM43 AD (1) 1 Z. mobilis CP4 Z. mobilis TISTR C. glutamicum KY9002 C. glutamicum 36 C 37 C 38 C Z4-80a (4), Z4-80b (5), Z4-80c (3), Z4-80d (4) 37 C: 80M (6) 39 C: 130M (12) 40 C: 200M (12) 40.5 C 41 C: C-2 (4) 41.5 C: C-2D (7) 41 C: FT-1 (5) 13; 3 4; Tn 4; 1; 2 6; (34-kbp) 1 4; 1
Zymomonas 2 2 LPS 2 ( 2) C. glutamicum LPS Zymomonas ( 3) 2 ( 2) ( 1)
Zymomonas ( 3) ( 1) ( 1) C. glutamicum Zymomonas C. glutamicum DNA ( 5) RNA ( 1) 4 3
Zymomomas 41 C45 C A. pasteurianus IFOTH-3 Turbidity (Kle% Unit) Z. mobilis TISTR 30 C 39 C Temperature Growth 45 C Ace4c acid 30 C 44 C Time (hr) K. marxianus NCYC 3 Acidity (w/v%) Time (hr) S. cerevisiae Z. mobilis. marxianus
A. pasteurianus Jasmine Rice Vinegar (MEXT-ARDA ) A. pasteurianus IFO 40 C (300L) NEDO!"#$%&'()*)+, K. marxianus?&"$',32:@2%"$"0&%,"0@2a#b, NEDO -",.%/"0"1"2,&3,40&"25#%&'&67,$%8,92%2"05,!%60%22:0%6;,<#1'$'&%6=&:%,>%0)2:/0"7;,?#$0'$%8, NEDO
Zymomonas Vector control A B C +X +X +Y +X +Z control K. marxianus NBRC 30 35 36 37 Parent strain D Gluconacetobacter 属菌 NBRC
( 1250 Temperature)() 40 35 30 25 1.18 kwh /24hr/L 0 10 20 0 10 3.17 kwh /24hr/L 20 1000 750 500 250 0 30 Growth (mv) 65% ( A. pasteurianus)
(Vinegar) Ethanol Acetate ~100% ΔH 0 = -493 KJ Glucose Glutamate 50~60% ΔH 0 = -1554 kj 233 kwh/kl (~10%) 426 kwh/kl (~10%) 30 C Glucose Ethanol ~100% ΔH 0 = -102 kj 3 kl 33 C 43 C 300 kwh / 41.2 kl 2010 600 kl 400 3000kL 8,600 kl 2010 ~1.2 kwh ~18 kwh ~128 kwh 30 C ~86 kwh H 2
80 C Thermophile 45 C Thermotolerant 反応 XY XX Mesophile 30 C X +XX 20 C
Zymomonas
5470807 26214 2012-210068 24924 2013-000843 2518 2014-159048,,,,,,,, 2684
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