J Life Sci Res 2014; 12: 15-19 2014 Osaka Prefecture University Journal of Life Science Research Online ISSN 2186-5809 583-8555 3-7-30 577-8567 3-1-1 2014 9 11 2014 10 21 Exposure mild-level LED light induces retina edema Miho KAWATE, Akemi KINOSHITA, Hideo SINAGAWA and Mariko YAMANO Department of Clinical Nutrition, School of Comprehensive Rehabilitation, Osaka Prefecture University, 3-7-30 Habikino, Habikino City, Osaka 583-8555, Japan; Department of Health Neutrition, Higashiosaka Junior College, 3-1-1 Higashiosaka City, Osaka 577-8567, Japan Received 11 September 2014; accepted 21 October 2014 Key words : LED blue light LED ; mouse retina ; edema LED 390 495 nm 380 nm 780 nm 1 LED 5 1 LED 2 LED 2 LED LED LED 3 50 1 4 Suzuki LED 5 LED 1 2 12 5 monocyte chemotactic protein-1 (MCP-1) LED 5 2 Suzuki C57BL/6J C57BL/6N LED LED E-mail: myamano@rehab.osakafu-u.ac.jp
16 2.1 7 C57BL/6N SLC 6 1 2 LED 1 LED1W 2 LED 2 LED2W 2 LED LED E-Bright Osaka, Japan 466 nm 1000 lux Fig. 1 1 12 8 20 MF 22 ± 2 C 25-61 Suzuki 5 2.2 200 mg/kg 4 0.1 M PFA PFA 30 0.1 M 12 μm 1 H&E vascular endotelial growth factor VEGF Santa Cruz, mouse IgG, 200 platelet-derived growth factor receptor beta PDGR-β Abcam, rabbit IgG, 100 glial fibrillary acidic protein GFAP Santa Cruz, goat IgG, 500 monocyte chemotactic protein-1 MCP-1: Abcam, rabbit IgG, 200 caspase-3 (Cell Signaling, rabbit IgG 500 Alexa-fluor 594 Alexafluor 488 Invitrogen VECTASHIELD 4,6-diamidino-2- phenylindole (DAPI) (Vector) G Alexa-fluor 594, F Alexa-fluor 488, DAPI V Cell Sense Fig. 1 LED LED 1000 lux 8 20 12 1 LED1W 2 LED2W LED 1 2 H&E Fig. 2 A C LED DAPI inner nuclear layer INL outer nuclear layer ONL LED DAPI Fig. 2D F LED1W INL 2 INL ONL H&E
17 Fig. 2 H&E DAPI A-C H&E A LED1W B LED2W C Scale bar = 50 μm D-F DAPI D INL, ONL LED1W E INL LED2W F INLONL GCL IPL INL OPL ONL Scale bar = 50 μm Fig. 3 LED2W Caspase-3 LED2W caspase-3 Scale bar = 50 μm Caspase-3 6 LED1W, LED2W caspase-3 Fig. 3 MCP-1 Fig. 4 MCP-1 LED VEGF 8 VEGF Fig. 4 GFAP Fig. 4 LED2W LED2W MCP-1 MCP-1 INL, OPL, ONL GFAP GCL VEGF Scale bar = 100 μm 7 GFAP LED ganglion cell layer GCL Fig. 4 H&E GCL PDGFR-β 8
18 Fig. 5 PDGFR-β PDGFR-β A GCL INL PDGFR-β LED2W C PDGFR-β B&D DAPI PDGFR-β Scale bar = 20 μm Fig. 5A GCL INL PDGFR-β Fig. 5C LED2W PDGFR-β inner plexiform layer IPL INL DAPI Fig. 5B&D LED LED 1 2, MCP-1 GFAP LED VEGF PDGFR-β PDGFR-β 2 LED 9, 10 Caspase-3 LED 11 LED LED 1. 12 LED 2 LED LED LED
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