1 2 3 4 DNA ms DNA 1) mt DNA 2) 2 DNA DNA 2011 3 5 3 1 DNA ALC 3) 523 820 822 2 DNA 3 4 msdna 5) Kai et al. (2005) 11 6) f61 f100 f60 f112 f86 f1770 f178 f160 f153 f204 f65 Cui et al. (2005) 3 Cst-6 Cst-7 Cst-8 2
Furukawa et al. (2004) fms13 Takagi et al. (2001) Tru-17 16 7) PCR 8) 3 DNA 3 9 10 HE 11) PIC 12) 13) ( ) 1 4 PIT 14) 2011 5 1 6 11 DNA mtdna msdna DNA 2011 7 6 11 78.03mm 61.32 92.80mm 11.46g 5.27 19.46g 0 22 2011 6 7 19.9 3 6 6 1.5 ALC 0 22 mtdna 0 22 msdna 3
43 32 38 19 23 23 0.939 0.974 1 76 33 1 15 300 2011 0 21 1 12 4 5 5 msdna 5 msdna16 43 32 38 17.2 19.0 HE 0.667 0.952 PIC 0.637 0.950 2 P>0.05 2 4
PI 2 8.2 10-28 2.5 10-27 7.3 10-29 76 2 3 2011 0 21 1 21 7 4 14 mtdna msdna mtdna 3 mtdna msdna msdna DNA mtdna msdna msdna PI 10 4 msdna16 mtdna Cui JZ, Shen XY, Yang GP, Gong QL, Gu QQ (2005) Characterization of microsatellite DNAs in Takifugu rubripes genome and their utilization in the genetic diversity analysis of T. rubripes and T. pseudommus. Aquaculture 250: 129-137. Furukawa S, Takeshima H, Otaka T, Mitsuboshi T, Shirasu K, Ikeda D, Kaneko G, 5
Nishida M, Watabe S (2004) Isolation of microsatellite markers by in silico screening implicated for genetic linkage mapping in Japanese pufferfish Takifugu rubripes. Fish. Sci. 70: 620-628. Kai W, Kikuchi K, Fujita M, Suetake H, Fujiwara A, Yoshiura Y, Ototake M, Venkatesh B, Miyaki K, Suzuki Y (2005) A Genetic Linkage Map for the Tiger Pufferfish, Takifugu rubripes. Genetics 171: 227-238. Reza S, Furukawa S, Mochizuki T, Matsumura H, Watabe S (2008) Genetic comparison between torafugu Takifugu rubripes and its closely related species karasu Takifugu chinensis. Fish. Sci. 74: 743-754. Shields GS, Kocher TD (1991) Phylogenetic relationship of North American Ursids based on analysis of mitochondrial DNA. Evolution 45: 218-221. Takagi M, Sakai H, Takei T, Kaneko Y (2001) Mechanism of inbreeding and genetic diversity of the Tiger puffer Takifugu rubripes. In: Japanese Fisheries Resource Conservation Association Report (in Japanese). Japanese Fisheries Resource Conservation Association, Tokyo, pp 41-47. 6
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mt DNA ms DNA DNA msdna mtdna DNA DNA (Microsoft, Microsoft Excel TM ) 1 11
msdna mtdna (Microsoft, Excel VBA TM ) Perl 76 msdna mtdna 21 1 DNA PIC 0.9 6 msdna f100, f160, f60, f1770, f204, f153 mtdna 300bp 800 ( 822 ) 98 16 msdna 820 822 mtdna mtdna 15 36 1 ( 1) Perl DNA DNA ( 2) 12
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