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1) SDS-PAGE 2) 3) 5mM SDS-PAGE 365nm 0.5mMDTT 11S (GA)(GB) Bowman-Birk (BBI) 7S Gly m Bd 30K 4) 33 11S 0.69 0.39 Bowman-Birk 7S 11S 11S 11S 11S Bowman-Birk 18
SH SS SDS-PAGE Monobromobimane mbbr 365nm mbbr SS CBB mbbr GA GB BBI Bowman-Birk GA GB BBI 1) Buchanan, B.B., Adamidi, C., Lozano, R. M., Yee, B. C., Momma, M., Kobrehel, K., Ermel, E., Frick, O. L., Thioredoxin-linked mitigation of allergic responses to wheat., Pro.Natl.Acad.Sci.USA 94 pp.5372-5377 1997 2) O Keefe, D. O., Quantitative electrophoretic analysis of proteins labeled with monobormobimane Analytical Biochemistry 222 86-94 (1994) 3) 51406-412 (2004) 4) Kohyama, K., Sano, Y., Doi, E., Rheological characteristics and gelation mechanism of tofu (soybean curd), J. Agric. Food Chem., 43, 1808-1812 (1995) 19