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1 Agilent Syed Salman Lateef Agilent Technologies, Inc. Bangalore, India ( C) ( ) Agilent Poroshell EC-C18 Agilent 126 Infinity LC (LOD).2 µg/ml 3 9 % Agilent 129 Infinity LC (UHPLC) UHPLC LOD 5 1

5 ( C) ( ) Agilent Poroshell EC-C18 Agilent 126 Infinity LC

2 (DHA) µg/ml 2,3 4 DHA AOAC DHA C UVDHA 22 nm ph nm 5 AOAC UV UV HPLC Milli Q (Millipore Elix 1 ) Lab-Scan ( ) Fluka () o- Fluka () Sigma-Aldrich () Agilent 126 Infinity LC Agilent 126 Infinity (G1312B) Agilent 126 Infinity (G1367E G133B) Agilent 126 Infinity (G1316A) Agilent 126 Infinity (G4212B) 1-mm Max- Light UHPLC Agilent 129 Infinity LC Agilent 129 Infinity (G422A) Agilent 129 Infinity (G4226A G133B) Agilent 129 Infinity (G1316C) Agilent 129 Infinity (G4212A) 1-mm Max- Light : Agilent Poroshell 12 EC-C x 1 mm 2.7µm ( ) : Agilent ChemStation B.4.2 2

Agilent 126 Infinity (G1367E G133B) Agilent 126 Infinity (G1316A) Agilent 126 Infinity (G4212B) 1-mm Max- Light UHPLC Agilent 129 Infinity LC Agilent

3 UHPLC 1 A 5, µg/ml (ppm) 5, ppm 1 ppm 1 A 2 A ph 2.5 ph o- 5 ml ph g 5 Agilent.2 µm ( ) A 5 µl 6 (RT) (RSD) 3 (LOD) (LOQ) Agilent 126 Infinity LC Agilent 129 Infinity LC TCC 2 ºC 2 ºC 4 Hz 4 Hz DAD (nm) mm 1 µl 1 mm 1 µl 4 ºC 4 ºC A 1 Agilent 126 Infinity LC Agilent 129 Infinity LC (µg/ml) (µg/ml) (µg/ml) mm (KH 2 PO 4 ) o- ph 2.5 B 6 % - 4 % 2 mm (KH 2 PO 4 ) o- ph % - 4 % ( ) %B ( ) %B ml/min 1.5 ml/min 5 µl 3. 4 µl 5. 3

5 21. 23. 243.5 1 mm 1 µl 1 mm 1 µl 4 ºC 4 ºC A 1 Agilent 126 Infinity LC Agilent 129 Infinity LC (µg/ml) (µg/ml) (µg/ml) 1 1 55.

4 ph 2.5 C 4 ( 2 % TCC 5 % 5 % 3 %) C 18 ppm 7, ppm 5 ppm HPLC UHPLC LOD LOQ RT RSD C A - Poroshell EC-C18 Agilent Poroshell EC-C18 TCC B 6 % 4 % ph nm 21. nm 23. nm 4 ºC Margolis % Margolis - 4 % MS ( ) ALS ( ) - ALS ALS 4 ºC % 1 % MS 1 Agilent 126 Infinity LC B 25 % Agilent Poroshell 12 EC-C18 23 nm 4

nm 4 ºC Margolis 6 22 89 % Margolis - 4 % 3 8 6 4 2 1.191 1.

5 (LOD) (LOQ) / (S/N) 3 LOD S/ N 1 LOQ S/N LOD LOQ LOD S/N = 3.5 µg/ ml LOQ S/N = 16.2 µg/ml 3 LOQ LOD LOQ LOQ.2 µg/ml µg/ml ( 1 ng) (LOQ ) S/N 16 mau y = 14.11x R 2 = (ppm) 3 1 µg/ml 225 µg/ml Sl : 1 LOD µg/ml S/N LOQ µg/ml S/N (µg/ml) R 2 n= L1=87 % (98 % 1 %) % 11 % L1 = 16 % % 3 5

2 µg/ml ( 1 ng) (LOQ ) S/N 16 mau 35 3 25 2 15 y = 14.11x + 15.542 R 2 =.9998 12.

6 (RT) RSD (%) RSD 1 (L1) 1.9 % RT RSD.13 % RT RSD RSD 4 RSD (%) µg/ml 7, µg/ml 5 µg/ml 4 (TCC ) % 3 % RSD (%) 6

7 : 1. ml/min 2 % : 1.2 ml/min :.98 ml/min 23 nm % % RT % % % RT % % RT TCC : 2 ºC 5 % : 21 ºC : 19 ºC : 5 µl 5 % : 5.25 µl : 4.75 µl : nm 3 nm : nm : nm % RT 5 % RT TCC UV DAD 3 (3 µg) (4, µg) (2 µg) (o- ph 2.5 ) (6 µg) (8, µg) (4 µg) (%) (5 3 ) / nm 3 9 % 7

2..5 5..2 6.1.2 : 21. 23. 243.5 nm 3 nm : 213. 233.3 246.5 nm : 27. 227. 24.5 nm 2.5.1.1 5.6.2 5.2.5 3.3.1.8 3.3.1 3.4.3 4 4 2 % RT 5 % RT TCC UV DAD 3 (3 µg) (4, µg) (2 µg) (o- ph 2.

8 3 (O- 1 O- 2 O- 3 ) ( 6) 3 O µg/ml 145 µg/ml O- 1 O- 3 E3 E33 ( ) µg/ml µg/ml 6 8 µg/ml O O- 2 O nm _ 2 2 _ 2 2 _ 2 2 _ 2 µg/ml O ± ± ±.7 O ± ± 43 ± O ± ± ± ( ) 23 nm

448 O- 1 12.512 O- 2 O- 3 5 23 nm 3 12 12.

9 UHPLC HPLC Agilent 129 Infinity LC UHPLC HPLC 25 UHPLC % UHPLC B 9 % 7 % 9 % 7 % Agilent 129 LC Infinity LOD.5 µg/ml LOQ.2 µg/ml (S/N =16) Agilent 126 Infinity LC 7 mau Agilent Poroshell 12 EC-C18 3. x 75 mm 2.7-µm UHPLC 6 y = x R 2 = (ppm) 7 Agilent 129 Infinity LC 1 µg/ml 225 µg/ml ( 5 ng 1125 ng) 9

2 µg/ml (S/N =16) Agilent 126 Infinity LC 7 mau 4 3 2 1 16 14 12 1 8.389.285 2.

10 HPLC RT RSD (%) 8 RSD (%) 2.5 % RT RSD.5 % Agilent Poroshell 12 EC-C % Agilent 129 Infinity LC 5 UHPLC RSD (%) RSD (%) UHPLC 6 1

11 1. INCHEM chemical safety information for intergovernmental organization: documents/cicads/cicads/cicad26. htm#subsectionnumber: US FDA, Food contamination and adultration: Data on Benzene in Soft Drinks and Other Beverages Data through May 16, FoodContaminantsAdulteration/ ChemicalContaminants/Benzene/ ucm55815.htm 3. L.K Gardner, G.D Lawrence, Benzene Production from Decarboxylation of Benzoic Acid in the Presence of Ascorbic Acid and a Transition-Metal Catalyst, J. Agric. Food. Chem, 41: , K. Zerdine; M.L. Rooney; J Vermue, The Vitamin C Content of Orange Juice packed in an Oxygen Scavenger Material, Food Chemistry, 82: , L. Novakova; P. Solich; D. Solichova, HPLC methods for simultaneous determination of ascorbic acid and dehy-droascorbic acids, Trends in Analytical Chemistry, 27: , S.A. Margolis; E. Park, Stability of Ascorbic Acid in Solutions Stored in Autosampler Vials, Clinical Chemistry, 47:

gov/food/foodsafety/ FoodContaminantsAdulteration/ ChemicalContaminants/Benzene/ ucm55815.htm 3. L.K Gardner, G.

12 Agilent Technologies, Inc., 211 Published in Japan, September 1, JAJP

2 (FDA) 1 1 A D E K 1 LC UV 15 HPLC 3.5 UHPLC Agilent 12 Infinity LC Agilent 12 Infinity (G1311B) Agilent 12 Infinity (G13E) Agilent 12 Infinity (G131

2 (FDA) 1 1 A D E K 1 LC UV 15 HPLC 3.5 UHPLC Agilent 12 Infinity LC Agilent 12 Infinity (G1311B) Agilent 12 Infinity (G13E) Agilent 12 Infinity (G131 1 Agilent 12 Infinity 1 Siji Joseph Agilent Technologies, Inc. Bangalore, India 12 1 2 4 5 3 2 1 4 1 12 14 4 2 Agilent 12 Infinity 2 4 5 3 2.5 1. 1.5 2. 2.5 3. 2 (RP-HPLC) Agilent Poroshell EC-C1 Agilent