Characteristic of protein error involving bromothymol blue Yuji Suzuki Bromothymol blue (BTB) hardly changed color from yellow to blue even by human serum albumin (HSA), but in the presence of Brij35, it changed from yellow to blue, which is called protein error. This phenomenon was investigated by an experiment and a calculation based on the chemical equilibrium of the protein error. The characteristic of this color change was sufficiently explained by the calculation. The color change of BTB from acid color to basic color was found to be caused by the increase in the molar absorptivity of the dye-protein complex produced by the reaction between BTB and HSA in the presence of Brij35. Bromothymol blue, Protein error, Color change
HD H D [H ][D ] [HD] KD P D [PD] PD KPD [P ][D ] HM H M [H ][M ] KHM [HM] P M [PM] PM KPM [P ][M ] P Y [PY] PY KPY [P ][Y ] [PS] P S PS KPS [P ][S] S HD [SHD] SHD KSD [S][HD]
CP CX KWKAKW 1 KB[H ]KB[H ] 2 [SHD] CD [D ] [D KD(CD ] KSDCS[H ] CX [D KDCD ] KSDCS[H ] CD[H ] [H] KD KSDCS[H ] (CD [H ] [H] KD KSDCS[H ] [SHD] CD [D ] [D KDCD ] [HD] CD [D ] [D KD(CD ) ] [HD] CD [D ] KDCD EDL KSDCS[H ] EHDL HDL(CD [D ]) EDS KDCD KSDCS[H ] EHDS HDS(CD [D ]) ESHDL SHDL[SHD] ESHDS SHDS[SHD] EBB EDL EHDL ESHDL EYB EDS EHDS ESHDS KD(CD [PD] EDL KSDCS[H ] EHDL HDL(CD [D ] [PD] [SHD] ) EPDL n DL[PD] KD(CD [PD] EDS KSDCS[H ] EHDS HDS(CD [D ] [PD] [SHD]) EPDS DS[PD] ESHDL SHDL[SHD] ESHDS SHDS[SHD] EBP EDL EHDL EPDL ESHDL EYP EDS EHDS EPDS ESHDS KDCD EDL EHDL HDL(CD [D ]) EDS KDCD EHDS HDS(CD [D ]) EBB EDL EHDL EYB EDS EHDS
KD(CD [PD] EDL EHDL HDL(CD [D ] [PD]) EPDL n DL[PD] EDS KD(CD [PD] EHDS HDS(CD [D ] [PD]) EPDS PDS[PD] EBP EDL EHDL EPDL EYP EDS EHDS EPDS