8~9 GSI 5cm 35.8g16.7~58.2g 63.0g20.7~124.2g 61.4g20.4 ~102.2g 17 GSI 1.1 0~5.2 4.0 0~17.7 1.9 0~9.6 1.2 0~4.5 0.60~2.6 0.50~2.2 67 66 76 60 3 9.5~27.5 8.9g1.7~9.6 3.6g1.3~6.3 5.3g 2.0~9.2 4.6g 2.1~8.1 7.9g3.2,12.6 16.2g13.8,18.6g GSI 9.5~27.5
80 1 2 3 30 4 5 10 mm 1 100 2 60L 15 5 PCR Flabobacterium psychrophilum 10 4060% F. psychrophilum 5 24 F. psychrophilum 1 5 2 F. psychrophilum 2 1 F. psychrophilu m 5 2 F. psychrophilum 3 F. psychrophilum 5 2 F. psychrophilum 4 1 3 3 F. psychrophilum 5 3 F. psychrophilum 5 F. psychrophilum 5 2 F. psychrophilum
1 18 0 16.721.8 17.640.5 0 620 3040 0.3370.597/
5.6km 2.1km 2 1m 0.3m/s 8km y3378.8x 2 222.34x+18889 y 400 80,000 80,000m 2 800003378.8x 2 222.34x+18889 x4.29 4.29m 3 /s 710 1.4
1.01 0.2km 2
4ml 25 30 30 9001,000rpm 0.075M KCl 15 1
400ppm Cl 5000ppm L COD TLM 250L 400ppm ml COD 100
20cm 25 20 1 2 10 ç 48 DO DO 0 2 COD 3 5 DO 25mg/L ph COD 10 10 10mg/L 20mg/L mg/l 30mg/L
H13 H14 H13 F1F1 F1 F2 H14 F1 BC1 BC1BC2 BC1 BC2 12,16,20 800L FRP BC2 60L 20 F1 BC2 BC2 DNA
DNA Aliah 2000 DNA DNA DNA DNA H14 32 27 H13 5 DNA PCR Crooijimans 1997MFW-1,12,18,29 Aliah 1999 Cca-21,30 5 NED,HEX,6-FAM PCR Aliah 1999 PCR PerkinElmer377DNA MFW-12,29 377DNA Cca-210.855,MFW-180.762 Cca-300.545 MFW-10.200 Cca-30 MFW-1 DNA MFW-12,29
Mycobacterium sp. PCR DNA 2001 12 % M. sp 2002 10 2003 24 PCR ISOGEN ISOGEN ISOGEN PCR PCR L 50L YNP-1 YNP-2Pao et al., 1990 94 65 7230 30 7210 EtBr % UV M. sp DNA M. tuberculosis DNA ISOGEN 160bp DNA ISOGEN ISOGEN 160bp DNA PCR PCR DNA DNA 75% DNA DNA
Koi herpesvirus KHV KHV KHV KHV 2002 12 30 10 30 480 84 PCR PCR ISOGEN DNA PCR PCR PCR L 40L KHV 9/5F 9/5RGilad et al., 2002 95 94 68 7230 39 72 EtBr 0.8% UV KHV DNAUSA R. P. Hedrick 484bp DNA KHV 100% 2001 KHV 2002 KHV 2002