1990Dr. Sackett EBMEvidence-Based Medicine EBMEBM DI EBMDI DI 2 Vol. 22 No. 72000
Evidence-Based Drug InformationEBDI EBDI IF IF EBDI EBDI EBDI EBDI Vol. 22 No. 72000 3
Y2K2000 1011 12 21 1998 Celera GenomicsCelera199910 2031.8 12 Celera40 102080 10 Incyte Pharmaceuticals National Institute of Health Department of Energy Human Genome Project1991 1994 Celera 1999112310 Whitehead Institute for Biomedical Research Genome Sequencing Center at the Washington UniversityBaylor College of MedicineJoint Genome Institute Sanger Centre 2005 2003 4 Vol. 22 No. 72000
Celera 6500 100300 6300 173 Human Genome Sciences6700 SNP Consortium, Ltd Expressed Sequence TagsESTs Single Nucleotide PolymorphismsSNPs 1000 1999 SNPs The SNP Consortium, LtdTSC Wellcome Trust1400 300 TSC Astra ZenecaBayerBristol-Myers Squibb Hoffmann-La RocheGlaxo-WellcomeHoechst Marion RousselMotorolaNorvatisPfizer SearleSmithKline Beecham Whitehead Institute for Biochemical ResearchWashington University s Genome Sequence CenterWellcome Trust s Sanger CentreStanford Human Genome CenterCold Spring Harbor Laboratory 1123SNP 2566SNPs TSC200130 SNPs15 URL Vol. 22 No. 72000 5
6 Vol. 22 No. 72000 http://snp.cshl.org/ http://www.ncbi.nln.nih.gov/genome/seq/ ftp://ftp.sanger.ac.uk/pub/human/sequences ftp://genome.wustl.edu/pub/gsc1 1999 Sanger Centre 23 3300 545 SNPs 102003 10 1996 13 1219 12 [(3)] 21 [(4)] 2001 cdna 14 2115 SNPs 2004 19996000 cdna 13 2200 2200 23
7 Vol. 22 No. 72000 hierachy a RNA Rational Drug Design Combinatorial Chemistry High-Throughput Screening Pharmacogenomics SNPs DNA99.9 0.1DNA 30 0.1300 SNPs
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9 Vol. 22 No. 72000 30 SNPs b SNPs Polymerase Chain ReactionPCRDNA DNA 15 35 CVD35 CVD35 E PCR
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cm 3.5mm mm 304025 linoleic acid linolenic acidoleic acid stearic acidpalmitic acid glogulin pentosan linamarin 4.5g pentosan g 36 Vol. 22 No. 72000
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DI REPORT 10 HIV DI 1981AIDS AIDS LAVHTLV-ARV 1986 HIV AIDS HIVHIV2 HIVHIV1 HIV2HIV1 HIV 120nm HIV HIV PAEIAHIV WB HIV HIV HIVHIV window periodsilent AIDS HIV HIVp24HIV gag PCRPCR PAparticle agglutination HIV HIV HIV EIAenzyme immunoassay ELISAEnzyme-linked immunosorbent assay EIA HIV Vol. 22 No. 72000 45
DI REPORT HIV HIV CLEIAchemiluminescent enzyme immunoassay EIA WBwestern blot immunoblotting method HIV SDSPAGE HIV1 Immunochromatography Assay HIV HIVHIV HIV HIV HIVHIV HIV PCRpolymerase chain reaction HIVDNA HIVCD4 HIVRNA DNADNA CD4DNA RNA CD4PCR DNA chain reaction HIVDNA RTPCRreverse transcription polymerase chain reaction HIVRNA HIVRNA HIVRNA HIV 97 31 Vol.53No6861995 Vol.51No6591993 992000 ABC AIDSATL HIV 46 Vol. 22 No. 72000
DI REPORT HIV12 PA HIV1 HIV2 2 210 1 HIV1 HIV2 555 286,000 H118 HIV12 PA PA HIV1 HIV2 2 210 1 205 70,000 555 154,000 H72 HIV12 EIA HIV1 HIV2 303 28 1 961 58,000 H101 HIV1HIV2 EIA HIV1 HIV2 AxSYM 28 1 HIV HIV 1 100 75,000 [] 4 230ml 45,000 H91 HIV1HIV2EIA PLUS EIA HIV1 HIV2 303 28 7 HIVHIV HIV OPDOPD 100 75,000 AIDSAIDS H87 AntiHIV12 EIA HIV1 HIV2 303 28 1 POD 192 108,400 HIV10 H86 HIV12 CLEIA HIV1 HIV2 1200 210 1 HIV12 84 80,000 AMPPD 100ml6 150,000 250ml6 30,000 300ml4 64,000 H93 Vol. 22 No. 72000 47
DI REPORT 1 2 12 HIV HIV 12 WB HIV1 28 1 WB HIV2 28 1 WB HIV1HIV2 130 3 28 1 WB HIV 28 4 HIV 1HIV2 15 130 1 HIV 1 21 2 HIV12HIV HIV 63 72,000 63 81,000 10 40,000 27 118,800 100 63,500 HIV1 H37 HIV2 H36 WB HIV12 HIV 1HIV2 H36 WBEIA AIDS HIV HIV H2 H111 48 Vol. 22 No. 72000
DI REPORT HIVEIA EIA HIV HIV p24 603 301 28 11 HIVIgG HIV OPDOPD 1N 100 160,000 H411 HIV1 PCR HIV1DNA 28 HIV 6 PN HIV DNA 10 HIV 96 195,000 HIV 96 135,000 HIV1DNA H64 HIV1 RT PCR HIV1 RNA 28 6 HIV1 QS HRP A B 10 24 95,000 HIV HIV H93 HIV EIA HIV1 OHIV2 HIVp24 100 28 HIV4HIV4 HIV4C1 HIV4C2HIV4 S1 60 48,000 HIV1HIV2 HIVp24 HIV H1112 125 Vol. 22 No. 72000 49
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immunolgobulin YFcIgGIgAIgMIgDIgE IgE T IgE IgG IgG MHC MHC T 58 Vol. 22 No. 72000
59 Vol. 22 No. 72000 MHC T T Y!!! 12TTTh1 12 BIgG T TTTh2 13131013 10B
TNF CDcluster of differentiation CD4TCD8T CD CD86TCD28 CD160 97T TMHC TMHCT TT B BY IIMHC 60 Vol. 22 No. 72000
BB MHCTT AIDS, CD, Ig, MHC, TNF AIDS acquired immunodeficiency syndrome CD cluster of differentiation Ig immunogloblin MHC major histocompatibility complex TNF tumor necrosis factor Vol.21 No.8 Vol.22 No.2 Vol.21 No.9 Vol.22 No.3 Vol.21 No.10 TDNA Vol.21 No.11 Vol.22 No.4 10Vol.22 No.5 Vol.21 No.12 11Vol.22 No.6 Vol.22 No.1 12Vol.22 No.7 Vol. 22 No. 72000 61
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23 10 07-378 12 PI2000-10, 10 1112 1212 13 147,938 vu 2000-20014,500 1220 25,555,333 1,394,932 24,160,401 Vol. 22 No. 72000 65
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