NanoVue_H1-4.indd

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えみおとじま
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1 NanoVue

2 NanoVue...6 NanoVue Sample... 7 Life Science DNA/RNA CyDye Protein UV UV Protein A280 A BCABradford LowryBiuret Utilities NanoVue PRINT VIA COMPUTER PVC E v PVC E v Print Via Computer PVC Print Via Computer PVC

3 % % 4.5 kg AC V Hz

4 4 English 2 abc 2 ABC C 1 1 _ 1 _ 1 3

5 1 0A/100%T : NanoVue : : ,4,5, Utilities p.26 4

6 1 5. Utility 2. Regional Language Japanese 2 5. Utility4. Preferences Quality Assurance On 2 2 Note: 1 Off On Quality Assurance On Quality Assurance On µl 2 µl 1 2 5

7 NanoVue

8 NanoVue sample µl Note >Lid Switch >Quality Assurance µl 8 >Lid Switch ,5 Auto-print USB Bluetooth

9 **. Lid Switch > **. Lid Switch Auto-Read Utility Preferences Lid Switch On / Methods Favorites / p.26 Utility RegionalJapanese English Utility Printer Utility Printer Auto-Print On Printer Built-in USB Computer<USB> Quality Assurance Utility Preferences Quality Assurance On 2 2 8

10 Life Science 6 DNA 1 DNA RNA 2 RNA Oligo 3 Tm Calculation 4 DNA Tm CyDye 5 cdna Protein 6 Life Science 10 mm 9

11 1. DNA/RNA 230 nm260 nm280 nm320 nm 260 nm 280 nm Abs260/Abs nm 230 nm Abs260/Abs230 EDTAGTC 2.0 DNA 50RNA nm 1 DNA 1 RNA mm0.5 mm AutomaticAuto 0.5 mm 0.2 mm AutomaticAuto C 3 2 Volume Diluent µl 99 µl Volume 1 Diluent 99 Dilution Factor nm 5 µg mlng µlµg µl µg ml Factor 10

12 6 DNA 50RNA nm260 nm280 nm 320 nm 2 Abs260/Abs280Abs260/Abs nm 9 DNA RNA 1.p nm 230 nm260 nm280 nm 320 nm 7. Sample Number: 1 8. Save Method: 9.Auto-Print: 11

2. CyDye DNA 1 CyDye 2 3 Cy3...550 nm Cy5...649 nm Fluorescein...495 nm 4 Coefficient µl mol-1cm-1 5 0.2 mm0.5 mm AutomaticAuto 0.5mm 0.

13 2. CyDye DNA 1 CyDye 2 3 Cy nm Cy nm Fluorescein nm 4 Coefficient µl mol-1cm mm0.5 mm AutomaticAuto 0.5mm 0.2 mm AutomaticAuto Dilution Factor... Factor...DNA DNA RNA Volume... µl Start RNA... RNA ng Background... Cy3 Cy5 430 nmfluorescein 400 nm 12

14 nm260 nm280 nm 3 A260/A280A260/ A230Adye/A nm DNA 4 Total DNA... cdna ng DNA Yield...Start RNA cdna Total cdna/start RNA 100 % Nucleotides... Nucleotides Dye Per Probe... pmol/ul 13

15 / 4. CyDye 7. Sample Number: 1 8.Save Method: 9.Auto-Print: / CyDye 14

16 Protein Protein UV UV BCABradford LowryBiuret 5 UVChristian Warburg A nm BSAIgG BCA 3-1Protein UV UV Protein A 280 A nm 260 nm Christian Warburg Biochemische Zeitung 310, mg ml=1.55 Abs Abs = 1 Abs Abs nm Abs 260 Abs Abs nm BSA 1= mg ml mg ml=1.115 Abs

17 Protein UV 1 1 Protein UV UV mm0.5 mm AutomaticAuto 0.5 mm 0.2 mm AutomaticAuto C 3 2 Volume Diluent µl 99 µl Volume 1 Diluent 99 Dilution Factor nm nm nm µg mlng µlµg µl 16

8 9 10 260 nm280 nm 320 nm 10 Protein UV 1.p.12 1 2. 3. 230 330 nm 230 nm260 nm280 nm 320 nm 7.

18 nm280 nm 320 nm 10 Protein UV 1.p nm 230 nm260 nm280 nm 320 nm 7. Sample Number: 1 8. Save Method: 9.Auto-Print: 17

Protein A 280 1 1 Protein UV UV 2 0.2 mm0.5 mm AutomaticAuto 0.5 mm 0.2 mm AutomaticAuto 3 1.00 9999 C 3 2 Volume Diluent 0.01 9999 1 µl 99 µl Volume 1 Diluent 99 Dilution Factor 100 1.

19 Protein A Protein UV UV mm0.5 mm AutomaticAuto 0.5 mm 0.2 mm AutomaticAuto C 3 2 Volume Diluent µl 99 µl Volume 1 Diluent 99 Dilution Factor nm 5 Christian WarburgBSAIgGLysozymeMolarextinction Mass ExtinctionE 1% Christian WarburgWarburg Christian mg/ml= 1.55 Abs 280 Abs 260 BSA10 mg/ml BSA mg/ml= Abs 280 IgG10 mg/ml IgG Lysozyme10 mg/ml Lysozyme 18

Molar extinction M -1 cm -1 Mass Extinction L g -1 cm -1 E 1%10 mg/ml1 % L g -1 cm -1 6 7 8 260 nm280 nm 320 nm 8

20 Molar extinction M -1 cm -1 Mass Extinction L g -1 cm -1 E 1%10 mg/ml1 % L g -1 cm nm280 nm 320 nm 8 Protein UV 1.p nm 230 nm260 nm280 nm 320 nm 7. Sample Number: 1 8. Save Method: 9.Auto-Print: 19

2-2BCABradford Lowry Biuret 2-1 Protein UV UV 4 BCA 2 1 BCA 562

Lowry 10 1500 µg/ml 5 100 µg/ml Biuret 1 15 mg/ml 130 mg/ml 1

21 2-2BCABradford Lowry Biuret 2-1 Protein UV UV 4 BCA 2 1 BCA 562 nm Bradford 595 nm BSA Lowry Folin-Ciocalteu phenol 750 nm BSA Biuret nm 4 BSA NanoVue BCA µg/ml µg/ml Bradford µg/ml µg/ml Lowry µg/ml µg/ml Biuret 1 15 mg/ml 130 mg/ml 1 BCA 2 Bradford 3 Lowry 4 Biuret 5 2 BCA 562 nm Bradford 595 nm Lowry 750 nm Biuret 546 nm

4 8 µg/mlµg/µlpmol/µlmg/dlmmol/lµmol/lg/lmg/lµg/l U/I%ppmppbconc DP 0 2 DP 5 DP 1 98768.

22 4 8 µg/mlµg/µlpmol/µlmg/dlmmol/lµmol/lg/lmg/lµg/l U/I%ppmppbconc DP 0 2 DP 5 DP Regression, Zero Regression, Interpolation, 3 Cubic Spline, 6 Standards Manual Standards p Manual p.23 p Standards 7-1 Replicates Off Standards Protein Standards C Std Calibration 2 Parameters 21

23 Calibration 11-1 Replicates 12-1 C C Replicates 13-1 OK Standards Next C 22

24 Proein 1.p Sample Number: 1 8. Save Method: 9.Auto-Print: 6 Manual Standards C 8-2 Calibration 2 Parameters 23

25 Calibration C 10-2 Standards Protein 1.p Sample Number: 1 8. Save Method: 9.Auto-Print: 24

26 4. Utilities 5 Utilities

27 4-1 Day Month Year Hour Minute Utilities 4-2,. Utilities 4-3 Built in USB Bluetooth Utilities 4-4 Games: Games Yes No Theme: History: Auto Standby: 1 2 Auto-Print: Auto-Print On 26

28 Quality Assurance: 2 Quality Assurance On Utilities 4-5 Utilities 4-6 Utilities 27

4-7NanoVue 1 :NanoVue Calibration Fluid 28-9244-05 1

NanoVue Calibration Fluid 3 Calibration Value NanoVue

Calibration Fluid 1 2% Status OK OK (C) 7 10 OK OK

29 4-7NanoVue 1 :NanoVue Calibration Fluid Utility 7 Pathlength Calibration 2 Certificate ID NanoVue Calibration Fluid 3 Calibration Value NanoVue Calibration Fluid Cal 4 OK 5 5 µl NanoVue Calibration Fluid 1 2% Status OK OK (C) 7 10 OK OK Utility 8 0.5mm 0.2mm 10 / 0.5mm 0.05mm 0.2mm 0.02mm 2.0% Utility 28

5. 4-1 Pathlength Calibration NanoVue Calibration Fluid

Applications2 Single Wavelength1 259nm 0.5mm 0.5 mm 0.

020 5 4 0.5mm 1 0.07mm Calibration Value 1/2 0.

30 Pathlength Calibration NanoVue Calibration Fluid Calibration Value 1 NanoVue [C] Path factor disabled 2 Applications2 Single Wavelength1 259nm 0.5mm 0.5 mm 0.2 mm 3 NanoVue Calibration Fluid Calibration Value 1/ mm mm Calibration Value 1/ Single Wavelength1 259nm 0.2mm 6 0.2mm mm Calibration Value 1/ [C] Path factor disabled

31 Spare Paper for Printer 20 rolls

32 1. PRINT VIA COMPUTER PRINT VIA COMPUTER PVC v PVC v PVC v CD-ROM PDF TEL: CD- ROM PDF CD-ROM GE TEL: FAX:

33 PVC E v1.5 USB USB USB ver2.0 OS Windows2000 Windows XP sp2 OS Administrator User / Guest PVC PC FTDI USB Serial Converter Drivers PVC CD-ROM Microsoft.NET Framework PC PVC Software FIDI USB Serial Converter Drivers USB Serial Port USB USB PC 32

34 1. CD-ROM PC PVC 2. setup.exe 3. ReadMe / 4. USB 5. PVC Software C:\Program Files\PVC\ Next 6. USB USB USB 6 7. Installation Complete Close 8. PC USB PC PC 9. USB to Serial ConverterUSB <-> Serial 10. USB Serial Port PC PVC PVC PVC Application 12. PVC Application file > Setup Com Port Printer Tools 13. PVC Application Option / / Microsoft ExcelTM, Microsoft WordTM PVC 33

35 PVC E v1.4 USB USB USB ver2.0 OS Windows2000 Windows XP sp2 OS Administrator User / Guest PVC PC FTDI USB Serial Converter Drivers PVC CD-ROM Microsoft.NET Framework PC PVC Software USB FIDI USB Serial Converter Drivers USB Serial Port USB USB PC 34

36 1. CD-ROM PC PVC 2. setup.exe 3. PC Microsoft.Net Framework 4 4. PVC Software C:\Program Files\PVC\ Next 5. PC USB PC PC 6. USB to Serial ConverterUSB <-> Serial 7. USB Serial Port 6 8. PC PVC PVC PVC Application 9. PVC Application file > Setup Com Port Printer Tools 10. PVC Application Option / / Microsoft ExcelTM, Microsoft WordTM PVC 35

37 Print Via Computer PVC v1.0 v1.1 Print Via Computer PVC NanoVue PCPC PVC PC PVC Viewer PVC PC OS Windows 2000 TM Windows XP TM OS 1. PC CD-ROM PC PC NanoVue 2. CD-ROM PC 3. PC OK Yes PC 5. PC 6. NanoVue PC USB 7. PC The Found New Hardware Wizard PVC PC NanoVue PC Found New Hardware PVC 8 36

38 PVC PC 1. NanoVue Utilities Auto-Print Printer USB 2. PC PVC 3. PVC COM COM 4. PVC PC NanoVue 1 Connected PC PVC Running 5. PVC Setup 6. PVC 1 option 2 PVC 19~20 7. PC PC Wave Scan NanoVue PC 8. print 9. PC PVC PC PC file Setup 10. PVC Viewer 37

39 1 2 38

40 60

41 OFF TEL : : : 30

42 FAQ Q&A Web FAQ Web FAQ Q&A 9:00 17: Life Sciences Academy lifesciences-ac.com GE Life Sciences Academy 8

NanoVuePlus_H1-4_CC2014.indd

NanoVuePlus_H1-4_CC2014.indd NanoVue Plus 71-3327-34 ... 2... 2... 2... 3... 4... 5... 6 NanoVue Plus...6 NanoVue Plus Sample... 7 Life Science... 9 1. DNA/RNA... 10 2. CyDye... 12 3.... 15 3-1Protein UV UV Protein A280 A280... 15