NanoVuePlus_H1-4_CC2014.indd

Size: px

Start display at page:

Download "NanoVuePlus_H1-4_CC2014.indd"

れれこびき
5 years ago
Views:

1 NanoVue Plus

2 NanoVue Plus...6 NanoVue Plus Sample... 7 Life Science DNA/RNA CyDye Protein UV UV Protein A280 A BCABradford LowryBiuret Utilities NanoVue Plus PVC E v Print Via Computer PVC

3 Pathlength Calibration Kit Pathlength Calibration Kit NanoVue plus HP or co.jp/technologies/spectro/nvu.asp % % 4.5 kg AC V50 60 Hz

4 4 English 2 a b c 2 A B C C 1 1 _ 1 _ 1 3

5 Esc 1 0A/100%T : NanoVue Plus : : ,4,5, Utilities p.26 Esc 4

6 1 5. Utility 2. Regional Language Japanese 2 5. Utility4. Preferences Quality Assurance On 2 2 Note: 1 Off On Quality Assurance On Quality Assurance On µl 2 µl 1 2 5

7 NanoVue Plus

8 NanoVue Plus Sample µl Note >Lid Switch >Quality Assurance µl 8 >Lid Switch ,5 Auto-print USB Bluetooth

9 Lid Switch Lid Switch Auto-Read Utility Preferences Lid Switch On / Methods Favorites / p.26 Utility RegionalJapanese English Utility Printer Utility Printer Auto-Print On Printer Built-in USB Computer<USB> Quality Assurance Utility Preferences Quality Assurance On 2 2 8

10 Life Science 6 DNA 1 DNA RNA 2 RNA Oligo 3 Tm Calculation 4 DNA Tm CyDye 5 cdna Protein 6 Life Science 10 mm 9

11 1. DNA/RNA 230 nm260 nm280 nm320 nm 260 nm 280 nm Abs260/Abs nm 230 nm Abs260/Abs230 EDTAGTC 2.0 DNA 50RNA nm 1 DNA 1 RNA mm0.5 mm AutomaticAuto 0.5 mm 0.2 mm AutomaticAuto C 3 2 Volume Diluent µl 99 µl Volume 1 Diluent Dilution Factor 100 Esc nm 5 µg/mlng/µlµg/µl µg/ml Factor 10

12 6 DNA 50RNA Esc nm260 nm280 nm 320 nm 2 Abs260/Abs280Abs260/Abs nm 9 DNA RNA Esc 1.p nm 230 nm260 nm 280 nm 320 nm 7. Sample Number: 1 8. Save Method: 9.Auto-Print: Esc 11

13 2. CyDye DNA 19 (8 CyDye Fluorescein10 Alexa) [Custom] 1 1. Life Sciences 5. CyDye DNA CyDye 2 [Custom] [Custom] 3 2-Custom-1 2-Custom-2 µl mol-1cm-1 2-Custom nm 0 2-Custom [None] 4 12

14 5 0.2 mm0.5 mm Automatic Auto Automatic Auto 0.5 mm 0.2 mm 6 OnOff Off 7 On 8 9 DNA µl None Factor Custom 12 13

15 13 14 FOIFrequency of Incorporation 1, / 4. DNA DNA 5. Sample Number 1 6. Save Method 7.A u t o - P r i n t / Esc 14

16 Protein Protein UV UV BCABradford LowryBiuret 5 UVChristian Warburg A nm BSAIgG BCA 3-1Protein UV UV Protein A 280 A nm 260 nm Christian Warburg Biochemische Zeitung 310, mg ml=1.55 Abs Abs = 1 Abs Abs nm Abs 260 Abs Abs nm BSAIgGLysozyme 15

Protein UV 1 1 Protein UV UV 2 0.2 mm0.5 mm AutomaticAuto 0.5 mm 0.2 mm AutomaticAuto 3 1.00 9999 C 3 2 Volume Diluent 0.

17 Protein UV 1 1 Protein UV UV mm0.5 mm AutomaticAuto 0.5 mm 0.2 mm AutomaticAuto C 3 2 Volume Diluent µl 99 µl Volume 1Diluent 99 Dilution Factor Esc nm nm nm µg/mlng/µlµg/µl 16

8 Esc 9 10 260 nm280 nm 320 nm 10 Protein UV Esc 1.p.16 1 2. 3. 230 330 nm 230 nm260 nm 280 nm 320 nm 7.

18 8 Esc nm280 nm 320 nm 10 Protein UV Esc 1.p nm 230 nm260 nm 280 nm 320 nm 7. Sample Number: 1 8. Save Method: 9.Auto-Print: Esc 17

Protein A 280 Christian Warburg BSAIgGLysozyme E1% 1% 1 1 Protein UV UV 2 0.2 mm0.5 mm AutomaticAuto 0.5 mm 0.

19 Protein A 280 Christian Warburg BSAIgGLysozyme E1% 1% 1 1 Protein UV UV mm0.5 mm AutomaticAuto 0.5 mm 0.2 mm AutomaticAuto C 3 2 Volume Diluent µl 99 µl Volume 1Diluent 99 Dilution Factor Esc nm 18

5 Christian WarburgBSAIgGLysozymeMolarextinction Mass ExtinctionE 1% Christian WarburgWarburg Christian mg/ml= 1.55 Abs 280 0.76 Abs 260 BSA10 mg/ml BSA 6.

20 5 Christian WarburgBSAIgGLysozymeMolarextinction Mass ExtinctionE 1% Christian WarburgWarburg Christian mg/ml= 1.55 Abs Abs 260 BSA10 mg/ml BSA 6.7 AU IgG10 mg/ml IgG 13.7 AU Lysozyme10 mg/ml Lysozyme 26.4 AU Molar extinction M -1 cm -1 Mass Extinction L g -1 cm -1 E 1%10 mg/ml1 % L g -1 cm -1 6 Esc nm280 nm 320 nm 8 Protein A280 Esc 19

21 1.p nm 230 nm260 nm280 nm 320 nm 7. Sample Number: 1 8. Save Method: 9.Auto-Print: Esc 20

3-2BCABradford Lowry Biuret 2-1 Protein UV

Lowry 4 Biuret 5 2 BCA 562 nm Bradford 595

22 3-2BCABradford Lowry Biuret 2-1 Protein UV UV 4 BCA BCA 2 1 BCA 562 nm BCA Bradford 595 nm BSA Lowry Folin-Ciocalteu phenol 750 nm BSA Biuret nm 4 1 BCA 2 Bradford 3 Lowry 4 Biuret 5 2 BCA 562 nm Bradford 595 nm Lowry 750 nm Biuret 546 nm

4 8 µg/mlµg/µlpmol/µlmg/dlmmol/lµmol/lg/lmg/lµg/l U/I%ppmppbconc DP 0 2 DP 5 DP 1 98768.

23 4 8 µg/mlµg/µlpmol/µlmg/dlmmol/lµmol/lg/lmg/lµg/l U/I%ppmppbconc DP 0 2 DP 5 DP Esc 3 5 Regression, Zero Regression, Interpolation, 3 Cubic Spline, 6 Standards Manual Standards p Manual p.23 p Standards 7-1 Replicates Off Standards Protein Esc Standards C Std Calibration 2 Parameters Esc 22

24 Calibration 11-1 Replicates 12-1 C C Replicates 13-1 OK Esc Standards Next 23

25 Esc Proein 1.p Sample Number: 1 8. Save Method: 9.Auto-Print: Esc 6 Manual Standards C 8-2 Calibration 2 Parameters Esc 24

26 Calibration C 10-2 Esc Standards Esc Protein 1.p Sample Number: 1 8. Save Method: 9.Auto-Print: Esc 25

27 4. Utilities 5 Utilities

28 4-1 Day Month Year Hour Minute Utilities Esc 4-2,. Utilities Esc 4-3 Built in USB Bluetooth Utilities Esc 4-4 Games: Games Yes No Theme: History: Auto Standby: 1 2 Auto-Print: Auto-Print On 27

29 Quality Assurance: 2 Quality Assurance On Utilities Esc 4-5 Utilities 4-6 Utilities 28

4-7NanoVue Plus 1 :NanoVue Plus Calibration Fluid

ID NanoVue Plus Calibration Fluid 3 Calibration Value

NanoVue Plus Calibration Fluid 1 2% Status OK OK (C) 7 10

30 4-7NanoVue Plus 1 :NanoVue Plus Calibration Fluid Utility 7 Pathlength Calibration 2 Certificate ID NanoVue Plus Calibration Fluid 3 Calibration Value NanoVue Plus Calibration Fluid Cal 4 OK 5 5 µl NanoVue Plus Calibration Fluid 1 2% Status OK OK (C) 7 10 OK OK Esc Utility 8 0.5mm 0.2mm 10 / 0.5mm 0.05mm 0.2mm 0.02mm 2.0% Esc Utility 29

5. 5-1 4-7: NanoVue Plus NanoVue Plus Calibration Fluid Calibration Value 1 4-7: NanoVue Plus 1. 4. 5. Utility 7. Pathlength Calibration 2 Cal 0.

31 : NanoVue Plus NanoVue Plus Calibration Fluid Calibration Value 1 4-7: NanoVue Plus Utility 7. Pathlength Calibration 2 Cal 0.5 mm 0.2 mm Cal mm / 2 = Abs 0.2 mm / 5 = Abs : NanoVue Plus 5. 5 µl 1 5 µl : NanoVue Plus 6. 30

0.5 mm 6 2. 0.020 Abs 0.5 mm 0.015 Abs 0.

2 mm 0.2 mm 1 5. 7 2. 0.020 0.5mm 0.015 0.

32 0.5 mm Abs 0.5 mm Abs 0.2 mm mm 0.5 mm mm mm 0.2 mm mm mm mm 0.5 mm mm 0.2 mm 5. 31

33 mm 0.02 Abs 0.2 mm Abs : NanoVue Plus

34 Spare Paper for Printer 20 rolls

35 33

36 6. Quality Assurance 10 mm 0.2 Abs 10 ng/µl 2 µl 3 5µl Quality Assurance 3 Quality Assurance µl NanoVue Plus

37 7. Cell holder obstructed (no light) Cell holder obstructed (low light) Cell holder obstructed (signal fail) Calibration problem (UV) on reference channel Calibration problem (IR) on reference channel. No light on reference channel Low light on reference channel 35

38 PVC E v1.5 USB USB USB ver2.0 OS Windows2000 Windows XP sp2 OS Administrator User / Guest PVC PC FTDI USB Serial Converter Drivers PVC CD-ROM Microsoft.NET Framework PC PVC Software FIDI USB Serial Converter Drivers USB Serial Port USB USB PC 36

39 1. CD-ROM PC PVC 2. setup.exe 3. ReadMe / 4. USB 5. PVC Software C:\Program Files\PVC\ Next 6. USB USB USB 6 7. Installation Complete Close 8. P C USB PC PC 9. USB to Serial ConverterUSB <-> Serial 10. USB Serial Port PC PVC PVC PVC Application 12. PVC Application file > Setup Com Port Printer Tools 13. PVC Application Option / / Microsoft ExcelTM, Microsoft WordTM PVC 37

40 Print Via Computer (PVC) PC 1. NanoVue Plus Utilities Auto-Print Printer USB 2. P C PVC 3. P V C COM COM 4. P V C PC NanoVue Plus 1 Connected PC PVC Running 5. PVC Setup 6. P V C 1 option 2 PVC 19~20 7. PC PC Wave Scan NanoVue Plus Esc PC 8. print 9. P C PVC PC PC file Setup 10. PVC Viewer 38

41 1 2 39

42 60

43 OFF TEL : : : 30

44 FAQ Q&A Web FAQ Web FAQ Q&A 9:00 17: Life Sciences Academy lifesciences-ac.com GE Life Sciences Academy 8

NanoVue_H1-4.indd

NanoVue_H1-4.indd NanoVue 71-2888-36 ... 2... 2... 2... 3... 4... 5... 6 NanoVue...6 NanoVue Sample... 7 Life Science... 9 1. DNA/RNA... 10 2. CyDye... 12 3.... 15 3-1Protein UV UV Protein A280 A280... 15 3-2BCABradford