[PDF] パーソナルスペクトルモニター GeneQuant pro 取扱説明書

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ふみなうばら
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1 GeneQuant pro GE imagination at work

2 DNARNA nm UV280 nm DNARNAoligo Base Sequence Tm Protein 595 Assay Protein 280 Measurement PC Windows... 25

.. 16 Base Sequence... 18 Tm... 19 Protein 595 Assay.

3 cal check

4 % % kg 5 cm / V 1 Instrument Ready 3

5 on0 off 3. 4

6 GeneQuant pro nm PCR DNARNA μg/mlng/μlμg/μlpmol/μlpmol phosphate 260 nm nm 260/ / nm PCR nearest neighbour Breslauer et al., Proc. Natl. Acad. Sci. U.S.A. 83, nm546 nm 562 nm BCA 600 nm PC Ecxel GLP PC 5

260/280 320 nm PCR nearest neighbour Breslauer et al., Proc. Natl.

7 128 64pixel set-up 28 3 Instrument Ready enter abs 12 set-up 14 set-up cal check 31 select print Base Sequence DNA RNA oligo PC base seq 18 Tm stop set ref enter 260 nm Tm 19 escape set-up 6

8 abs nm DNA nm DNA 260/ /230 RNA nm RNA 260/ /230 oligo nm / 260/ /230 protein 595 assay Set-up nm set-up protein 280 meas nm cell culture 600 nm OD 7

230260280320 nm / 260/280 260/230 protein 595 assay Set-up

9 DNARNA DNA RNA 10 mm 260 nm μg/ml 33 μg/ml Base Sequence = A 260 DNARNA mm set-up 27 μg/ml set-up ng/μlμg/μlpmol/μl pmol phosphate pmol/μl PCR 1 μg/ml = 1 ng/μl = μg/μl pmol/μl = μg/ml 1,000 pmol phosphate = μg/ml 315 DNA MW MWg/mol= da 312.2dC 288.2dG 328.2dT MW RNA dt 303.2dU ' 3' 17 2 MW 61 MW g/molna23.0k 39.1TEA

ng/μlμg/μlpmol/μl pmol phosphate pmol/μl PCR 1 μg/ml = 1 ng/μl = 0.

10 260/280 DNA RNA nm 280 nm 280 nm 260 nm 260/ nm 280 nm nm 230 nm Tris EDTA RNA 260/ RNA nm RNA 260/280260/ mm set-up 9

11 nm 320 nm set-up A 260 A 280 A 320 = A 260 A 320 = A 260 A 320 /A 280 A 320 = A 260 A 320 /A 230 A SWIFT nm 230 nm 260 nm280 nm 320 nm 10

12 nm 595 nm BSA 546 nm BCA bicinchoninic acidbca 562 nmbca nm

13 UV280 nm 280 nm A nm Christian Warberg Biochemisceh Zeitung 310, nm mg/ml = 1.55 A A 260 = 1 A A nm 320 nm set-up nm nm nm BSA 1 = mg/ml mg/ml= A 280 A 280 HiTrap 12

76 A 260 = 1 A 280 2 A 260 280 nm 320 nm set-up 2 260280 nm 2

14 600 nm OD OD OD OD 1OD 600 = cells / ml GeneQuant pro OD OD OD 2.0 set-up 10 mm 13

15 set-up set-up abs select enter stop No Sample No. 11 PrinterSerialPC None PCAuto Print print PrintSurvey Scan DNARNAoligo EnglishDeutschFrancaisEspanol Italiano 14

16 base seqtmabsdnarnaoligoprotein 595 assayprotein 280 meascell culture set ref enter... set up print PC print 15

17 DNARNAoligo 8 Pathlength mm Units) 320 nm Use 320nm Dilution factor Insert sample μg/mlng/μlμg/μlpmol/μlpmol pmol/μlpmololigo 320 nmuse 320 nm 1 10,000 set ref enter DNA/RNA nm 260/230260/ stop oligo Default Factor Calculated Factor Use Default Factor Insert sample 33 μg/mlssdna 37 μg/ml set ref enter oligo 260/230260/280 stop 16

pmol/μlpmololigo 320 nmuse 320 nm 1 10,000 set ref enter DNA/RNA 230260280320 nm

18 nm nm select set-up select Auto enter DNA Determination Date : 28 May 2003 Name : Factor : 50.0 Units : μg/ml Pathlength : 10 mm Dilution Factor : 1 Use 320 nm : No No. 230 nm 260 nm 280 nm 320 nm 260/ /230 Conc Oligonucleotide Determination Date : 28 May 2003 Name : Dflt Factor : 33.0 Units : μg/ml Pathlength : 10 mm Dilution Factor : 1 Use 320 nm : No No. 230 nm 260 nm 280 nm 320 nm 260/ /230 Conc

471 0.255 ----- 1.850 2.045 23.5 Oligonucleotide Determination Date : 28 May 2003 Name : Dflt Factor : 33.

19 Base Sequence 8 Base Type) Phosphorylated Counter Ion) MW Other MW Theor. Abs MW Calculated MW) Calculated Factor DNARNA NaKTEA Other Other 1.0 1,000.0 enter ACG T/U 10 64delete cancelselect select AU/μmol pmol/μl μg/ml Base Sequence Date : 28 May 2003 Name : AGC, AGC, AGC, AGC, AGC Base Type : DNA Phosphorylated : Yes Counter Ion : Na Theor. Abs : Calculated MW : Calculated Factor :

0 enter ACG T/U 10 64delete cancelselect select AU/μmol pmol/μl μg/ml Base Sequence Date : 28 May

20 Tm 8 10 mer Primer Conc. Buffer Molarity Tm Calculated Tm Insert sample pmol/μl nearest neighbourtm set ref enter Tm enter Tm Tm nearest neighbour stop Tm Date : 28 May 2003 Name : AGC, AGC, AGC, AGC, AGC Phosphorylated : Yes Counter Ion : Na Primer Conc. : 1.0 Buffer Molaryty : 0.1 Calculated Tm : 57.0 deg.c No. 260 nm Meas. Tm

May 2003 Name : AGC, AGC, AGC, AGC, AGC Phosphorylated : Yes Counter Ion : Na Primer Conc.

21 Protein 595 Assay 8 Method No. of Standards Wavelength Pathlength Units New Standards? Std#1 Insert Std#1 Std#2 Insert Std#2 Insert Sample Bradford 595Biuret 546BCA mm μg/mlmg/mlμgmg/ml set ref enter enter enter enter Y set ref enter stop 20

22 Protein 595 Date : 28 May 2003 Name : Method : Bradford No. of Standards : 6 No. Abs. Conc Slope : Corre. Coeff : Intercept : No Abs. Conc

23 Protein 280 Measurement 8 1Coeff 1 2Coeff nm Use 320 nm Insert sample 280 nm nm nm set ref enter protein 280 means stop Protein 280 nm Measurment Date : 28 May 2003 Name Coeff. 1 : Coeff. 2 : Use 320 nm : No : No. 260 nm 280 nm 320 nm Conc

24 8 Factor Insert sample set ref enter cell culture 600 nmod stop Cell Culture 600 nm Date : 28 May 2003 Name : Factor : 2.0 No. OD600 Result

25 >2.0A Absorbance >2.0A Invalid Curve Solution too dilute Too much light 1 Ref.1 error 2 Ref.2 error Lamp failure Protein 595Cell Culture2.000 A (100 μg/ml0.1 mg/ml) Lamp too hot Filter error Grating error 24

26 Seiko DPU Seiko DPU-414 DIP SW On print Seiko DPU-414 set-up Print PC Windows Excel 2Hyperterminal Serial Hyperterm.exe GeneQunat pro OK Com 1 OK COM1 / 19,2008 1OK ASCII ASCII OK 2 25

27 Excel 26

28 10 mm GeneQuant pro 15 mm 8.5 mm ng/μl 1 μl mm > 2, > > > μl UV > ,500 3 > dsdna 10 mmabs260 = μg/ml = ng/μl DNA PCR ng/μl 7 μl dsdna 2 μg/ml μl 14 ng dsdna dsdna 20 μg/ml μl 60 ng dsdna 70 μl UV RNA DNA 70 μl 27

29 GeneQuant pro 5 nm 10 mm 20 mm μl μl μl UV μl 28

30 10 mm mm 0.5 mm 0.1 M NaOH 0.1 M HCl Decon nm A

32 GLP/GMP ISO 9001 cal check Good Laboratory PracticeGLP GLP nm nm 260 nm NIST

33 / 1.6AT5 mm 20 mmfst2 32

34 A B 2 5 C B 2 A

35 Fixed at 230, 260, 280, 320, , 595 and 600 nm Survey scan : nm Czerny - Turner configuration with 1,200 lines/mm holographic grating Automatic upon switch on 5 nm 1 nm 0.5 nm Long life borosilicate glass pulsed duterium lamp Silicon photodiode 0 to A for 230, 260, 280 and 320 nm 0 to A for , 595 and 600 nm 1.0% or A to A, whichever is the greater 0.5% of absorbance value < 0.1% T at 220 nm using Acetone Centronics parallel as standard 9 pin serial via interface adapter lead mm 4 kg V AC, 50/60 Hz, 80 VA Safety standard EN EMC emissions EN Generic emissions part 1 EMC immunity EN Generic immunity part 1 Susceptibility standard IEC 801 Quality System Designed and manufactured in accordance with an ISO 9001 approved quality system 34

36 12 Biochrom Ltd., 22 Cambridge Science Park, Milton Road, Cambridge CB4 0FJ, UK 35

37 2006 GE Home Page (NS)

NanoVuePlus_H1-4_CC2014.indd

NanoVuePlus_H1-4_CC2014.indd NanoVue Plus 71-3327-34 ... 2... 2... 2... 3... 4... 5... 6 NanoVue Plus...6 NanoVue Plus Sample... 7 Life Science... 9 1. DNA/RNA... 10 2. CyDye... 12 3.... 15 3-1Protein UV UV Protein A280 A280... 15