Fusarium oxysporum PCR 281 Fusarium oxysporum PCR Fusarium oxysporum Schlechtend. : Fr F. oxysporum F. oxysporum Formae speciales : f. sp. 150 F. oxys
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- さや なかきむら
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1 81 Fusarium oxysporum PCR Fusarium oxysporum Schlechtend. : Fr F. oxysporum F. oxysporum Formae speciales : f. sp. 150 F. oxysporum Solanum lycopersicum F. oxysporum F. oxysporum f. sp. lycopersici FOL 1 3 f. sp. radicis-lycopersici FORL 4 Fusarium 1 olecular Phylogeny and PCR-based Differentiation of the Pathogenic Types of Fusarium oxysporum in Tomato. By Yasushi HIRANO and Tutomu ARIE Fusarium oxysporum PCR F. oxysporum PCR I Polygalacturonase 80 F. oxysporum endopolygalacturonase pg1, pg5 exopolygalacturonase pgx1, pgx F. oxysporum ClustalW NJ 1pg1 1,585 1,587 bp pg5 1,873 bp pgx1 1,794 1,813 bp pgx4 1,375 bp F. oxysporum pg UTRs UTR UTR polygalacturonase PG pgx1 4 PG HIRANO and ARIE, 009 II 1 4 PG F. oxysporum 35
2 t polygalacturonase AT1 1AT1 36
3 83 1 uni a unif unir sp13 b sp13 f sp13 r sp3 c sp3 f sp3 r sprl d sprlf sprlr 5 ATCATCTTGTGCCAACTTCAG 3 5 GTTTGTGATCTTTGAGTTGCCA 3 5 GTCAGTCCATTGGCTCTCTC 3 5 TCCTTGACACCATCACAGAG 3 5 CCTCTTGTCTTTGTCTCACGA 3 5 GCAACAGGTCGTGGGGAAAA 3 5 GATGGTGGAACGGTATGACC 3 5 CCATCACACAAGAACACAGGA 3 nt of Saitama ly1 pg1 nt of Saitama ly1 pg1 nt of Saitama rly pgx4 nt of Saitama rly pgx4 nt of Saitama ly1 pg1 nt of Saitama ly1 pg1 nt of Saitama rly pgx4 nt of Saitama rly pgx4 a Amplifies 67 bp fragment from ff. sp. lycopersici and radicis lycopersici b Amplifies 445 bp fragment from f. sp. lycopersici races 1 and 3 c Amplifies 518 bp fragment from f. sp. lycopersici races and 3 d Amplifies 947 bp fragment from f. sp. radicis lycopersici pg1 pgx4 pg1 FOL 1 3 FORL f. sp. melongenae f. sp. cucumerinum DNA uni FOL FORL 67 bp sp3 FOL bp FOL 1 FORL pg1 pgx4 sp13 FOL bp sprl FORL 947 bp 1 4 uni PCR 1 3 sp13 PCR 1 3 sp3 PCR 3 sprl PCR 4 uni sp13 sp3 sprl PCR 4 F. oxysporum PCR uni f. sp. conglutinans f. sp. tulipae f. sp. dianthi f. sp. melonis F. oxysporum 67 bp f. sp. colocasiae f. sp. fragariae f. sp. lactucae f. sp. lagenariae f. sp. niveum f. sp. raphani f. sp. phaseoli f. sp. spinaciae f. sp. tracheiphilum sp bp 3 sp3 518 bp sprl 37
4 Primer set 947 bp 67 bp 518 bp 445 bp FOL FORL race 1 race race 3 uni sp13 sp3 sprl uni sp13 sp3 sprl uni sp13 sp3 sprl uni sp13 sp3 sprl uni uni f. sp. cucumerinum f. sp. melongenae B 67 bp Primer set uni sp13 sp3 sprl FOL race 1 FOL race FOL race 3 FORL PCR f. sp. colocasiae AFF f. sp. conglutinans AFF f. sp. cucumerinum AFF f. sp. dianthi AFF f. sp. fragariae AFF f. sp. lactucae AFF f. sp. lagenariae AFF f. sp. melongenae AFF B f. sp. melonis AFF 3051 f. sp. niveum AFF f. sp. phaseoli AFF 3577 f. sp. raphani AFF f. sp. spinaciae AFF f. sp. tracheiphilum AFF 3575 f. sp. tulipae AFF NPF Fo PCR 947 bp PCR PCR PCR DNA DNA 5 70 uni PCR bp 56 uni DNA DNA PCR DNA g/ l 6 DNA pg/ l DNA 1.0 pg/ l 5 38
5 85 4 F. oxysporum f. sp. F. oxysporum f. sp. lycopersici race 1 F. oxysporum f. sp. lycopersici race F. oxysporum f. sp. lycopersici race 3 F. oxysporum f. sp. radicis lycopersici F. oxysporum f. sp. colocasiae F. oxysporum f. sp. conglutinans F. oxysporum f. sp. cucumerinum F. oxysporum f. sp. dianthi F. oxysporum f. sp. fragariae race 1 1 race 1 11 race 5 race 3 9rly race 1 3 F. oxysporum f. sp. lactucae F. oxysporum f. sp. lagenariae F. oxysporum f. sp. melongenae F. oxysporum f. sp. melonis F. oxysporum f. sp. niveum F. oxysporum f. sp. phaseoli F. oxysporum f. sp. raphani F. oxysporum f. sp. spinaciae F. oxysporum f. sp. tracheiphilum F. oxysporum f. sp. tulipae F. oxysporum f. sp. lycopersici uni universal primer set FToFO9701 f. sp. lycopersici race 3 Saitama rly f. sp. radicis lycopersici AFF f. sp. melonis sp13 race 1 and 3 specific primer set FToFO9701 Saitama rly sp3 race and 3 specific primer set FToFO9701 Saitama rly sprl rly specific primer set FToFO9701 Saitama rly 4 PCR 39
6 DNA pg/ l uni primer set FToFO9701 f. sp. lycopersici race sp13 primer set FToFO9701 sp3 primer set FToFO9701 sprl primer set Saitama rly f. sp. radicis lycopersici 5 DNA PCR Saitama ly1 Saitama ly Saitama ly1 Saitama ly 1 1 uni sp13 sp3 sprl uni sp13 sp3 sprl uni sp13 sp3 sprl uni sp13 sp3 sprl uni sp13 sp3 sprl 67 bp 518 bp 445 bp 6 PCR PCR DNA 1.0 pg/ l PCR PCR cm 1 DNA PCR 1 0 F. oxysporum 6 1 DNA DNA PCR 67 bp 445 bp uni sp13 sp3 sprl 7 40
7 87 1 uni sp13 sp3 sprl uni sp13 sp3 sprl 67 bp 445 bp 7 PCR 1 PCR HIRANO and ARIE, PCR 0 PCR HIRANO, Y. and T. ARIE 006 : J. Gen. Plant Pathol. 7 : : icrobes Environ. 4 : /03/17 DDVP /03/ /03/ / 03/4 EP /03/ / 03/ /03/ /03/ / 03/7 P 355 DL 1/03/04 EDDP 1700 DL1/3/ /03/ /03/ /03/ H 1/03/ L 1/03/
「諸雑公文書」整理の中間報告
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1 2 (90cm 70cm 2015) 3 (68cm 28cm 30cm 12kg 2015) (77.5 109.5cm 2015) 4 (22cm 50cm 50cm 4.6kg 2015) (45cm 62.5cm 2015) (47.4cm 62.5cm 2014) 5 (28.5cm 23.5cm) (45cm 62cm 2015) (97cm 107cm 2015) 6 7 8 9
10 117 5 1 121841 4 15 12 7 27 12 6 31856 8 21 1983-2 - 321899 12 21656 2 45 9 2 131816 4 91812 11 20 1887 461971 11 3 2 161703 11 13 98 3 16201700-3 - 2 35 6 7 8 9 12 13 12 481973 12 2 571982 161703 11
