YK170 17β-Estradiol EIA キット
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1 YK Estradiol EIA FOR RESEARCH LABORATORY USE ONLY FAX: TEL: Website: ask@yanaihara.co.jp
2
3 YK170: 17 -Estradiol EIA. 20 (E1) (E2) (E3) YK Estradiol EIA 17-1) pg/ml 2) 41 duplicate 3) ) 5)SA-HRP 6) 100 µl 7)OPD 1 8 8) 9) 10) ) 2
4 IgG HRP HRP - - HRP 17-3
5 IgG ng SA-HRP 12 ml 1 HRP ml % 0.1 M ( ph 5.0 ) 7. OPD 2 o ml 1 1M ml ml 1 1% Tween
6 . ( ) µl 1 ml) 8 12 ( 490 nm (492 nm ) 2.5 ) (1000 ml) ml 0.1 ml 9.9 ml 4,000 pg/ml 0.2 ml 0.4 ml 1,333 pg/ml pg/ml 0 pg/ml 16.5 pg/ml 4,000 pg/ml 16.5 pg/ml 16.5 pg/ml 5.5 pg/ml 5.5 pg/ml 16.5 pg/ml 6 ml 6 ml 5
7 ml OPD 1 50 ml ( ) 950 ml (20 30 ) 350 µl µl 100 µl 50 µl SA-HRP 100 µl 2 7. OPD µl µl 490 nm 492 nm 13. or 5 Parameter Log-Logit Log (linear ) 17-6
8 pg/ml - 7
9 VI O.D. (490 nm) β-Estradiol (pg/ml) Added 17β-Estradiol Observed Expected Recovery (%) B Added 17β-Estradiol Observed Expected Recovery (%) Added 17β-Estradiol Observed Expected Recovery (%)
10 B Added 17β-Estradiol Observed Expected Recovery (%) Concentration (pg/ml) A B Dilution Ratio Concentration (pg/ml) a b Dilution Ratio 9
11 B Concentration (pg/ml) a b Dilution Ratio (%) 17β-Estradiol 100 Testosterone 0.28 Estrone 0.94 Progesterone 0.01 d-aldosterone 0.00 Estriol 0.16 (+)-4-Androsterone-3,17-dione 0.02 Trans-Androsterone 0.02 Mestranol 0.14 Ethynylestradiol Methoxyestradiol 1.79 Hexestrol Estradiol 17-(β-D-Glucronide) Estradiol 3-Glucronide 17-Sulfate Estradiol 3-(β-D-Glucronide) Estradiol 3-Sulfate Estradiol 3-Sulfate 17-Glucronide Estradiol 3,7-Disulfate 0.00 Cholesterol 0.00 CV(%) CV(%) CV(%) CV(%)
12 Goda, Y. et al. : Development of the ELISA for detection of hormone-disrupting chemicals. WATER SCIENCE TECHNOLOGY 42(7-8): 81-88, Goda, Y. et al. : Development of the ELISA for detection of estrogenic hormones in environment. IWA 2nd World Water Congress, Berlin, Germany IWA CD-ROM: 269, Nichols, D. J. et al. : Runoff of estrogen hormone 17 -estradiol from poultry litter applied to pasture. JOURNAL OF ENVIRONMENTAL QUALITY 26(4): , FAX: TEL: ask@yanaihara.co.jp
YK052 Mouse Leptin ELISA キット
YK052 Mouse Leptin ELISA 取扱説明書 研究用試薬 FOR RESEARCH LABORATORY USE ONLY 株式会社矢内原研究所 418-0011 静岡県富士宮市粟倉 2480-1 FAX: 0544-22-2770 TEL: 0544-22-2771 Website: www.yanaihara.co.jp E-mail: ask@yanaihara.co.jp 目次
YK250 Human GIP (Active) ELISA FOR RESEARCH LABORATORY USE ONLY FAX: TEL: Website: E-mai
YK250 Human GIP (Active) ELISA FOR RESEARCH LABORATORY USE ONLY 418-0011 2480-1 FAX: 0544-22-2770 TEL: 0544-22-2771 Website: www.yanaihara.co.jp E-mail: ask@yanaihara.co.jp . 2. 3. 4. 56. 7. 812. 12. 1213
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3--2-3-Iodo-2-propynyl butylcarbamate (IPBC) Carbamic acid, butyl-, 3-iodo-2-propynyl ester Iodocarb CAS 55406-53-6 C 8 H 12 NO 2 I C (g/cm 3 ) (mmhg) log P ow 281.09 (280.9910) 64 68 1.51 1.57 (20 C)
平成26年度 化学物質分析法開発報告書
N,N- N,N-Dimethylacetamide Acethyldimethylamine CAS 127-19-5 C 4 H 9 NO 87.12 ~ 87.14 87.68413 163-165 C 1) - 18.59 C 2) 1 mg/l 25 C 3) 3.3 hpa 2 C 4) log P ow -.77 2).9429 2/4 C 1) 3.1 4) 1.31E - 8 atm-m
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平成26年度 化学物質分析法開発報告書
2,2-2,2 -Iminodiethanol Diethanol amine CAS 111-42-2 C 4 H 11 NO 2 105.1356-105.0790-28 C 1) 1.0881 g/cm 3 1)
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平成26年度 化学物質分析法開発報告書
2,2-2,2 -Iminodiethanol Diethanol amine CAS 111-42-2 C 4 H 11 NO 2 105.1356-105.0790-28 C 1) 1.0881 g/cm 3 1)
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