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1 2007 Time required for developing protective immunity against coldwater disease in cultured Ayu Plecoglossus altivelis altivelis after vaccination Hideo HARA* Plecoglossus altivelis altivelis Flavobacterium psychrophilum MC MC MC g PH L - ml/cgygg glphml rpm mlml/cgy ml FKB CFU/mL FKBrpm / FKB FKB ISA-A FA L FKB Montanidae IMS IMS IMSFKB MCMC DKF-% mlimsfkbml gml gmlml ml MC *
2 MC kgmcg MC MC PHmL/CGY ml rpmml ml/cgyml CFU/mL/CGY CFU/mL CFU/mL ml L mmmmmm L/ Fisher Relative percent survival :RPS=- /Cory and Amend g CFU/mL CFU/mL CFU/mL CFU/mL CFU/mL RPS P RPS- RPS - P RPS-- P RPS
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4 アユ冷水病ワクチンの効果発現時期 2 1 無処理対照区 注射ワクチン区 経口ワクチン区 3日後攻撃 攻撃菌数 CFU/mL 5 3日後攻撃 攻撃菌数 CFU/mL) 7日後攻撃 攻撃菌数 CFU/mL 7日後攻撃 攻撃菌数 CFU/mL 日後攻撃 攻撃菌数 CFU/mL 日後攻撃 攻撃菌数 CFU/mL 図1 試験1における攻撃試験のの推移
5 RPS(%) ワクチン 投 与 完 了 から 攻 撃 開 始 までの 日 数 RPS RPS(%) ワクチン 投 与 完 了 から 攻 撃 開 始 までの 日 数 RPS
6 アユ冷水病ワクチンの効果発現時期 2 1 無処理対照区 注射ワクチン区 経口ワクチン区 3日後攻撃 攻撃菌数 CFU/mL 3日後攻撃 攻撃菌数 CFU/mL 2 1 7日後攻撃 攻撃菌数 CFU/mL 14日後攻撃 攻撃菌数 CFU/mL 日後攻撃 攻撃菌数 CFU/mL 図3 試験2における攻撃試験のの推移
7 gg MC g g g RPS RPS MC RPS % MC MC RPS g Oncorhynchus mykiss g MC gg g MC g MC Wakabayashi H Toyama T and Iida T A study on serotyping of Cytophaga psychrophila isolated from fishes in Japan Fish Pathol Rahaman M H Ototake M Iida Y Yokomizo Y and Nakanishi T Efficacy of oil-adjuvanted vaccine for coldwater disease in ayu Plecoglossus altivelis Fish Pathol Rahaman M H Ototake M and Nakanishi T Water-soluble adjuvants enhance the protective effect of Flavobacterium psychrophilum vaccines in ayu Plecoglossus
8 altivelis Fish Pathol - CORY T R and AMEND D F Immunization of sockeye salmononcorhynchus nerka against vibriosis using the hyperosmotic infiltration techniqueaquaculture : : -
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More information2004 3 16 3 ( 2003 6 ) Lethenteron reissneri Anguilla japonica Hypomesus nipponensis Plecoglossus alteviles altivelis Salangichthys microdon Salvelimus leucomaenis leucomaenis Salvelimus leucomaenis pluvius
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