Table 1 Liver function and serum amylase levels after the ligation of the common bile duct in rats (93)

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2 Table 1 Liver function and serum amylase levels after the ligation of the common bile duct in rats (93)

Fig. 1 Serum insulin (IRI) and plasma glucagon (IRG) levels after the ligation of the common bile duct. Fig. 3 Electron micrograph of the acinar cells at 2nd weeks after ligation. ( ~5,000).

3 Fig. 1 Serum insulin (IRI) and plasma glucagon (IRG) levels after the ligation of the common bile duct. Fig. 3 Electron micrograph of the acinar cells at 2nd weeks after ligation. ( ~5,000). A lot of zymogen granules (Z) were preserved and intracellular organells were well developed. N: Nucleus, G: Golgi Apparatus, Mt: Mitochondria, rer: rough Endoplasmic Reticulum, Lu: Acinar lumen Fig. 2 Light micrograph of the pancreas at 2nd weeks after ligation (H-E Staining, ~200) (94)

4 Fig. 6 Light micrograph of pancreas at the 4th Fig. 4 Electron micrograph of B cells at 2nd weeks after ligation. ( ~10,000). Many of B cells preserved a lot of secretory granules and their intracellular organelles were well developed. Sg: Secretory granule, rer: rough Endoplasmic Reticulum, G: Golgi apparatus, Mt: Mitochon dria weeks after ligation, (H-E Staining, ~100) Fig. 5 Electron micrograph of B cells at 2nd weeks after ligation. ( ~30,000) Granular mem branesfused with each other and (ƒ -shaped exocytosis ( ª) were often observed. Ca: Capil larylumen, Sg: Secretory granule (95)

Fig. 7 Electron micrograph of acinar cells at the 4th weeks after ligation. Acinar lumen ( ª) became narrow and the acinar parenchyma became atrophic with marked decrease of zymogen granules.

5 Fig. 7 Electron micrograph of acinar cells at the 4th weeks after ligation. Acinar lumen ( ª) became narrow and the acinar parenchyma became atrophic with marked decrease of zymogen granules.( ~5,000). N: Nucleus, rer: rough Endoplasmic Reticlum Fig. 9 Electron micrograph of A cells at the 4th weeks after ligation. Many of A cells showed enlargement of the Golgi apparatus and juvenile secretory granules were observed around them. ( ~8,000) N: Nucleus, G: Golgi apparatus, rer: rough Endoplasmic Reticulum. Fig. 8 Electron micrograph of B cells at the 4th weeks after ligation. Many of B cells showed degenerative changes: decrease in their secretory granules, swollen cisternae of the rough endoplas micreticulum and appearance of vacuoles. ( ~ Fig. 10 Electron micrograph of A cells at the 4th weeks after ligation. ( ~25,000). ƒ -shaped exocytosis ( ª) were often observed at the capil larylumen. Sg: Secretory granuled, Ca: Capillar ylumen 10,000). V: Vacuole, Ly: Lysosome, Mt: Mito chondria,sg: Secretory granule (96)

6 Table 2 Diameter and number of zymogen granules in acinar cells. (mean }SD) (* p<0.05) (97)

3) Opie EL: Cholelithiasis and pancreatic disease. Amer J Med Sci 121: 27, 1901 4) Kelly TR: Gallstone pancreatitis: Patho physiology.

8 3) Opie EL: Cholelithiasis and pancreatic disease. Amer J Med Sci 121: 27, ) Kelly TR: Gallstone pancreatitis: Patho physiology.surgery 80: 488, ) Thurston OG: Pathogenesis of gallstone pan creatitis.amer J Surg 120: 301, ) ŽO î Ž, ¼: _ Î Ç ÆäX Š. ŠO ÈŽ Ã, 3: 355, ) Luft JH: Improvements in epoxy resin embed dingmethods. J Biophys Biochem Cytol 9: 409, ) Tuzhilin SA, et al: Hepatic lesions in pan creatitisclinicoexperimental data. Amer J Gastroent 64: 108, ) Zieve L, et al:: Secretion of pancreatic enzyme. III. Response of patients with cirrhosis to se cretinand pancreozymin. Am J Dig Dis 12: 303, ) Ermak TH, et al: Zymogen granules of pan creasdecrease in size in response to feeding. Cell Tissue Res 214: 51, ) Starzl YE, et al: The effect of diabetes mellitus on portal blood hepatotrophic factors in dogs. Surg Gynecol Obstet 140: 549, ) Bucher NLR, et al: Regulation of hepatic regeneration in rats by synergetic action of insulin and glucagon. Proc Nat Acad Sci 72: 1157, ) c Œd, ¼: äx à ª å ŠŠÖ- B [-ƒ Ž ŽhŒƒ Ž Ì Ý ð ß Á Ä. ˆãŠw Ì ä Ý, 99: 179, ) Marco J: Elevated plasma glucagon level in cirrhosis of the liver. N Engl J Med 289: 1107, ) Scherwin RS: Hyperglucagonemia in cirrhoss: Altered secretion and sensitivity to glucagon. Gastroenterology 74: 1224, ) Muller WA, et al: The influence of the antece dentdiet upon glucagon and insulin secretion. New Eng J Med 285: 1450, ) Felig P, et al: Concentration of glucagon and

9 ELECTRON MICROSCOPIC OBSERVATION ON THE PANCREAS AFTER THE EXPERIMENTAL LIGATION OF THE COMMON BILE DUCT IN RATS Keiho SHIM, Ichikun KO, Euichol KIM, Katsusuke SATAKE and Kaoru UMEYAMA 1st Department of Surgery, Osaka City University Medical School The present examination has been attempted to investigate morphological changes of pancreatic acinar and islet cells after complete ligation of common bile duct in rats using electron microscope. At the early stage of the ligation of the common bile duct (2nd weeks after the ligation), the most of acinar cells and B cells of the Langerhans islands showed hyperfunctional appearance, but no remarkable changes were observed in the A cells. At the late stage (4th weeks after the ligation), acinar cells became atrophied and B cells were degenerative changes, however many of A cells showed hyperfunctional appearance. (100)

Ÿ ( ) ,166,466 18,586,390 85,580,076 88,457,360 (31) 1,750,000 83,830,000 5,000,000 78,830, ,388,808 24,568, ,480 6,507,1

Ÿ ( ) ,166,466 18,586,390 85,580,076 88,457,360 (31) 1,750,000 83,830,000 5,000,000 78,830, ,388,808 24,568, ,480 6,507,1 ( ) 60,000 120,000 1,800,000 120,000 100,000 60,000 60,000 120,000 10,000,000 120,000 120,000 120,000 120,000 1,500,000 171,209,703 5,000,000 1,000,000 200,000 10,000,000 5,000,000 4,000,000 5,000,000