Tox
|
|
|
- きみかず いいはた
- 5 years ago
- Views:
Transcription
1 CytoTox 96 Non-Radioactive Cytotoxicity Assay No. TB163J G1780 I. 2 II. 2 III. A. 4 B. CytoTox 96 Assay 4 IV. A. 5 B. 5 C. LDH 5 V. A. 7 B. 8 C. LDH 8 D. 9 VI. A. 11 B. 12 VII. 13 VIII. 14 IX. A. 15 B. 15 Tel prometec@jp.promega.com 1
2 I. CytoTox 96 Non-Radioactive Cytotoxicity Assay 51 Cr CytoTox 96 Assay RI 51 Cr テート(lactate dehydrogenase; LDH)LDH(INT) (30) 96 (diaphorase) LDH(1) 51 Cr () (2,3) CytoTox 96 (4) (5 8) (9) CytoTox 96 Assay 51 Cr i) ii) iii) 96ELISA CytoTox 96 Assay NAD + + LDH + NADH Diaphorase NADH + INT NAD + + ( ) II. CytoTox 96 Non-Radioactive Cytotoxicity Assay 1,000 G vials Substrate Mix 60ml Assay Buffer 25µl LDH Positive Control 3ml Lysis Solution (10 ) 65ml Stop Solution : Substrate Mix Assay Buffer -20 Substrate Mix 6~8LDH Positive ControlLysis Solution (10)Stop Solution 46 Tel prometec@jp.promega.com 2
3 ( ) Effector Cell Spontaneous LDH Release Control Target Cell Spontaneous LDH Release Control Target Cell Maximum LDH Release Control Lysis Solution (10 ) Volume Correction Control Culture Medium Background Control 250 g Target Cell Maximum LDH Release Control Lysis Solution(10 ) 250g4 LDH 50µl ( ) 5,000 LDH Positive Control Assay Buffer Substrate Mix Substrate Mix 50µl 30 50µl Stop Solution 490nm 1. CytoTox 96 Non-Radioactive Cytotoxicity Assay Tel prometec@jp.promega.com 3
4 III. A. 2(LDH) CytoTox 96 Assay (V.D) LDH LDH AB LDH(3) 5% 1% BSA B. CytoTox 96 Assay CytoTox 96 Assay5 #2 #3( LDH LDH) 51 Cr3 LDH # 1. Effector Cell Spontaneous LDH Release( LDH ) : LDH # 2. Target Cell Spontaneous LDH Release( LDH ): LDH # 3. Target Cell Maximum LDH Release( LDH ) : 100% LDH # 4. Volume Correction Control( ) : Lysis Solution (10 ) # 5. Culture Medium Background( ) : LDH Tel prometec@jp.promega.com 4
5 IV. (YAC-1K562Daudi) LDHCytoTox 96 Assay S/N ( 2) LDH Positive Control LDH ( IX.A ) V 96 : PBS + 1% BSA ( ) A. 1. V µl/ 50µl/100µl/ 2. Culture Medium Background 3 4 : Target Cell Maximum LDH Release Lysis Solution (10) / Volume Correction Control 3. : LDHLDH Positive Control 2µl10ml PBS(1% BSA) (5,000) 34 B µl Lysis Solution(10 ) 10µl % CO g 4 C. LDH 1. 50µl Assay Buffer12ml2037 Assay Buffer 37 12ml Assay BufferAssay Buffer Substrate Mix : : Substrate Mix -20 6~8 3. Substrate Mix50µl 30 Tel prometec@jp.promega.com 5
6 4. Stop Solution 50µl 5. Stop Solution1 490nm 492nm 6. Culture Medium Control2 : 1 100µl 100µl 150µl LDH (05,00010,00020,000 cells/100µl) V µl Lysis Solution( / ) 250 g 4 50µl Assay Buffer Substrate Mix 1 50µl Substrate Mix 30 50µl Stop Solution 490nm 2. Tel prometec@jp.promega.com 6
7 V. A Effector Cell Spontaneous LDH Release ( LDH ): LDH 34 ( ) 2. : V96( 4 ) : µl / 3. Target Cell Spontaneous LDH Release (LDH ): 3 4 ( 4 ) ( ) 4. Target Cell Maximum LDH Release ( LDH ):34(4) 100µl10µlLysis Solution(10 ) Triton X %Lysis Solution45 5. Volume Correction Control ():100µl() µl Lysis Solution(10 ) Lysis Solution(10) 6. Culture Medium Background ( ): µl LDH 7. :LDH Positive Control (LDH ): (LDH) LDH Positive Control 2µl 10ml PBS + 1% BSA (5,000 )5,000 LDH Positive Control13,500L g4 Tel prometec@jp.promega.com 7
8 3. CytoTox 96 Non-Radioactive Cytotoxicity Assay CytoTox 96 Assay 3 4 B % CO Target Cell Maximum LDH Release 100µl 10µl Lysis Solution(10 ) : 5µl Lysis Solution (10 ) g 4 C. LDH 1. 50µl( ) 2. Assay Buffer 12ml Assay Buffer 37 12ml Assay Buffer 12ml Assay Buffer Substrate Mix µlSubstrate Mix µl Stop Solution 5. Stop Solution 1 490nm 492nm Tel prometec@jp.promega.com 8
9 D. 1. Target Cell Spontaneous LDH ReleaseEffector Cell Spontaneous LDH Release Culture Medium Background 2. Target Cell Maximum LDH Release ControlVolume Correction Control : % = Effector Spontaneous Target Spontaneous 100 Target Maximum Target Spontaneous CytoTox 96 Assay 4 : C3H/HeJ NK/LAK IL-2(500ng/ml) 5 : YAC-1 : RPMI mM HEPES + 5% FBS : 96 : 1 50µl 10,000 : 50µl 10:1 0.02:1 : 5%CO CytoTox 96 Non-Radioactive Cytotoxicity Assay Tel prometec@jp.promega.com 9
10 1. 10:1 ( ) Culture Medium Background ( ) = = Target Spontaneous ( ) Culture Medium Background ( ) = = Effector Spontaneous ( ) Culture Medium Background ( ) = = Target Maximum ( ) Volume Correction Control ( ) = = % = Effector Spontaneous Target Spontaneous 100 Target Maximum Target Spontaneous % = = 77.3% ( 10:1 ) Tel prometec@jp.promega.com 10
11 VI. A. CytoTox 96 Assayテート LDH (SDScetrimide) CytoTox 96 Assay Lysis Solution 100µl 15µl Lysis Solution(10 )( 9% Triton X-100) 3745~60 (50µl)96Substrate Mix(50µl) µl Stop Solution ELISA 490nm LDH Y490nmX5 Lysis Solution(10 ) 37 45~60 50µl ( ) 5,000 LDH Positive Control Assay Buffer Substrate Mix Substrate Mix 50µl 30 50µl Stop Solution 490nm 5. CytoTox 96 Non-Radioactive Cytotoxicity Assay Tel prometec@jp.promega.com 11
12 B. CytoTox 96 Assay ( ) (10)N-methyl-Daspartate(NMDA)(11) CytoTox 96 Assay 6 NMDA HEK 293 (12) 20 LDH LDH LDH LDH Substrate Mix (50µl) 30 Stop Solution 490nm % = Experimental LDH release (OD490) Maximum LDH release (OD490) 6. NMDA (11) CytoTox 96 Non-Radioactive Cytotoxicity Assay ( ) Tel prometec@jp.promega.com 12
13 VII. Effector Cell Spontaneous LDH Release Control Target Cell Spontaneous LDH Release Control LDH Culture Medium Background Control LDH AB LDH 5% 1% BSA Culture Medium Background Control (< cells/ml) G 250 g (%) (%) LDH (%) 4 6~8 ( V.B.3) LDH ( V.C.3) 15~20 490nm 492nm LDH ( V.C.2 V.C.3) Target Cell Maximum Release ( IV) Tel prometec@jp.promega.com 13
14 VIII. 1. Nachlas, M.M. et al. (1960) The determination of lactic dehydrogenase with a tetrazolium salt. Anal. Biochem.1, Korzeniewski, C. and Callewaert, D.M. (1983) An enzyme-release assay for natural cytotoxicity. J. Immunol. Meth. 64, Decker, T. and Lohmann-Matthes, M.L. (1988) A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity. J. Immunol. Meth. 115, Brander, C. et al. (1993) Carrier-mediated uptake and presentation of a major histocompatibility complex class I-restricted peptide. Eur. J. Immunol. 23, Behl, C. et al. (1994) Hydrogen peroxide mediates amyloid beta protein toxicity. Cell 77, Lappalainen, K. et al. (1994) Comparison of cell proliferation and toxicity assays using two cationic liposomes. Pharm. Res. 11, Allen, M.J. and Rushton, N. (1994) Use of the CytoTox 96 Assay in routine bio-compatibility testing in vitro. Promega Notes 45, Sinensky, M.C., Leiser, A.L. and Babich, H. (1995) Oxidative stress aspects of the cytotoxicity of carbamide peroxide: in vitro studies. Toxicol. Lett. 75, Moravec, R. (1994) Total cell quantitation using the CytoTox 96 Non-Radioactive Cytotoxicity Assay. Promega Notes 45, Singer, C.A. et al. (1999) The mitogen-activated protein kinase pathway mediates estrogen neuroprotection after glutamate toxicity in primary cortical neurons. J. Neurosci. 19, Miroslav, C. et al. (1995) Using Promega s CytoTox 96 Non-Radioactive Cytotoxicity Assay to measure cell death mediated by NMDA receptor subunits. Promega Notes 51, Gorman, C.M., Gies, D.R. and McCray, G. (1990) Transient production of proteins using an adenovi rus transformed cell line. DNA Prot. Eng. Technol. 2, 3. Tel prometec@jp.promega.com 14
15 IX. A. PBS + 1% BSA 0.2g/l KCl 8.0g/l NaCl Lysis Solution (10 ) 9% (v/v) Triton X g/l KH2PO4 1.15g/l Na2HPO4 1%(w/v) bovine serum albumin Stop Solution 1M acetic acid B. CellTiter 96 Non-Radioactive Cell Proliferation Assay 1,000 G4000 5,000 G4100 CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay (a) 1,000 G5421 5,000 G ,000 G5440 CellTiter 96 AQueous MTS Reagent Powder (a) 1g G mg G1112 CellTiter 96 AQueous One Solution Cell Proliferation Assay (a) 200 G3582 1,000 G3580 5,000 G3581 (a)the MTS tetrazolium compound is the subject of U.S. Pat. No. 5,185,450 assigned to the University of South Florida and is licensed exclusively to Promega Corporation Promega Corporation. All Rights Reserved. CellTiter and CytoTox 96 are trademarks of Promega Corporation and are registered with the U.S. Patent and Trademark Office. Triton is a registered trademark of Union Carbide Chemicals and Plastics Co., Inc. Tel prometec@jp.promega.com 15
SC-85X2取説
I II III IV V VI .................. VII VIII IX X 1-1 1-2 1-3 1-4 ( ) 1-5 1-6 2-1 2-2 3-1 3-2 3-3 8 3-4 3-5 3-6 3-7 ) ) - - 3-8 3-9 4-1 4-2 4-3 4-4 4-5 4-6 5-1 5-2 5-3 5-4 5-5 5-6 5-7 5-8 5-9 5-10 5-11
<4D6963726F736F667420506F776572506F696E74202D208376838C835B83938365815B835683878393312E707074205B8CDD8AB78382815B83685D>
i i vi ii iii iv v vi vii viii ix 2 3 4 5 6 7 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60
活用ガイド （ソフトウェア編）
(Windows 95 ) ii iii iv NEC Corporation 1999 v P A R T 1 vi P A R T 2 vii P A R T 3 P A R T 4 viii P A R T 5 ix x P A R T 1 2 3 1 1 2 4 1 2 3 4 5 1 1 2 3 4 6 5 6 7 7 1 1 2 8 1 9 1 1 2 3 4 5 6 1 2 3 4
I
I II III IV V VI VII VIII IX X XI XII XIII XIV 1. 2 3 4 5 2. 6 7 8 3. 1 2 3 9 4 5 10 6 11 4. 1 2 3 1 2 12 1 2 3 1 2 3 13 14 1 2 1 15 16 1. 20 1 21 1 22 23 1 2 3 4 24 1 2 ok 25 1 2 26 1 2 3 27 2. 28
困ったときのQ&A
ii iii iv NEC Corporation 1997 v P A R T 1 vi vii P A R T 2 viii P A R T 3 ix x xi 1P A R T 2 1 3 4 1 5 6 1 7 8 1 9 1 2 3 4 10 1 11 12 1 13 14 1 1 2 15 16 1 2 1 1 2 3 4 5 17 18 1 2 3 1 19 20 1 21 22 1
III
III 1 1 2 1 2 3 1 3 4 1 3 1 4 1 3 2 4 1 3 3 6 1 4 6 1 4 1 6 1 4 2 8 1 4 3 9 1 5 10 1 5 1 10 1 5 2 12 1 5 3 12 1 5 4 13 1 6 15 2 1 18 2 1 1 18 2 1 2 19 2 2 20 2 3 22 2 3 1 22 2 3 2 24 2 4 25 2 4 1 25 2
iii iv v vi vii viii ix 1 1-1 1-2 1-3 2 2-1 3 3-1 3-2 3-3 3-4 4 4-1 4-2 5 5-1 5-2 5-3 5-4 5-5 5-6 5-7 6 6-1 6-2 6-3 6-4 6-5 6 6-1 6-2 6-3 6-4 6-5 7 7-1 7-2 7-3 7-4 7-5 7-6 7-7 7-8 7-9 7-10 7-11 8 8-1
これわかWord2010_第1部_100710.indd
i 1 1 2 3 6 6 7 8 10 10 11 12 12 12 13 2 15 15 16 17 17 18 19 20 20 21 ii CONTENTS 25 26 26 28 28 29 30 30 31 32 35 35 35 36 37 40 42 44 44 45 46 49 50 50 51 iii 52 52 52 53 55 56 56 57 58 58 60 60 iv
パワポカバー入稿用.indd
i 1 1 2 2 3 3 4 4 4 5 7 8 8 9 9 10 11 13 14 15 16 17 19 ii CONTENTS 2 21 21 22 25 26 32 37 38 39 39 41 41 43 43 43 44 45 46 47 47 49 52 54 56 56 iii 57 59 62 64 64 66 67 68 71 72 72 73 74 74 77 79 81 84
これでわかるAccess2010
i 1 1 1 2 2 2 3 4 4 5 6 7 7 9 10 11 12 13 14 15 17 ii CONTENTS 2 19 19 20 23 24 25 25 26 29 29 31 31 33 35 36 36 39 39 41 44 45 46 48 iii 50 50 52 54 55 57 57 59 61 63 64 66 66 67 70 70 73 74 74 77 77
untitled
i ii iii iv v 43 43 vi 43 vii T+1 T+2 1 viii 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 a) ( ) b) ( ) 51
2
1 2 3 4 5 6 7 8 9 10 I II III 11 IV 12 V 13 VI VII 14 VIII. 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 _ 33 _ 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 VII 51 52 53 54 55 56 57 58 59
平成18年版 男女共同参画白書
i ii iii iv v vi vii viii ix 3 4 5 6 7 8 9 Column 10 11 12 13 14 15 Column 16 17 18 19 20 21 22 23 24 25 26 Column 27 28 29 30 Column 31 32 33 34 35 36 Column 37 Column 38 39 40 Column 41 42 43 44 45
活用ガイド （ソフトウェア編）
(Windows 98 ) ii iii iv v NEC Corporation 1999 vi P A R T 1 P A R T 2 vii P A R T 3 viii P A R T 4 ix P A R T 5 x P A R T 1 2 3 1 1 2 4 1 2 3 4 5 1 1 2 3 4 5 6 6 7 7 1 1 2 8 1 9 1 1 2 3 4 5 6 1 2 3 10
活用ガイド (ソフトウェア編)
ii iii iv NEC Corporation 1998 v vi PA RT 1 vii PA RT 2 viii PA RT 3 PA RT 4 ix P A R T 1 2 3 1 4 5 1 1 2 1 2 3 4 6 1 2 3 4 5 7 1 6 7 8 1 9 1 10 1 2 3 4 5 6 7 8 9 10 11 11 1 12 12 1 13 1 1 14 2 3 4 5 1
エクセルカバー入稿用.indd
i 1 1 2 3 5 5 6 7 7 8 9 9 10 11 11 11 12 2 13 13 14 15 15 16 17 17 ii CONTENTS 18 18 21 22 22 24 25 26 27 27 28 29 30 31 32 36 37 40 40 42 43 44 44 46 47 48 iii 48 50 51 52 54 55 59 61 62 64 65 66 67 68
01_.g.r..
I II III IV V VI VII VIII IX X XI I II III IV V I I I II II II I I YS-1 I YS-2 I YS-3 I YS-4 I YS-5 I YS-6 I YS-7 II II YS-1 II YS-2 II YS-3 II YS-4 II YS-5 II YS-6 II YS-7 III III YS-1 III YS-2
MultiPASS Suite 3.20 使用説明書
TM MultiPASS Suite Ver.3.20 for Windows ii iii Copyright 2000 Canon Inc. ALL RIGHTS RESERVED iv v vi vii viii ix x 1 1 1-1 1 1 2 3 1-2 4 5 1 1-3 1 6 1-4 7 1 8 9 1-5 10 1 11 1-6 1 1-7 1 1-8 2 1 1-9 1 1
ii iii iv CON T E N T S iii iv v Chapter1 Chapter2 Chapter 1 002 1.1 004 1.2 004 1.2.1 007 1.2.2 009 1.3 009 1.3.1 010 1.3.2 012 1.4 012 1.4.1 014 1.4.2 015 1.5 Chapter3 Chapter4 Chapter5 Chapter6 Chapter7
MultiPASS B-20 MultiPASS Suite 3.10使用説明書
TM MultiPASS Suite Ver.3.10 for Windows ii iii Copyright 1999 Canon Inc. ALL RIGHTS RESERVED iv v vi vii viii ix x 1 2 3 4 5 6 7 8 9 xi xii 1 1 1-1 1 2 3 1-2 4 5 1 1-3 6 1-4 1 7 8 1-5 9 10 11 1-6 1 1-7
入門ガイド
ii iii iv NEC Corporation 1998 v P A R 1 P A R 2 P A R 3 T T T vi P A R T 4 P A R T 5 P A R T 6 P A R T 7 vii 1P A R T 1 2 2 1 3 1 4 1 1 5 2 3 6 4 1 7 1 2 3 8 1 1 2 3 9 1 2 10 1 1 2 11 3 12 1 2 1 3 4 13
3 5 18 3 5000 1 2 7 8 120 1 9 1954 29 18 12 30 700 4km 1.5 100 50 6 13 5 99 93 34 17 2 2002 04 14 16 6000 12 57 60 1986 55 3 3 3 500 350 4 5 250 18 19 1590 1591 250 100 500 20 800 20 55 3 3 3 18 19 1590
i ii iii iv v vi vii ( ー ー ) ( ) ( ) ( ) ( ) ー ( ) ( ) ー ー ( ) ( ) ( ) ( ) ( ) 13 202 24122783 3622316 (1) (2) (3) (4) 2483 (1) (2) (3) (4) (5) (6) (7) (8) (9) (10) (11) 11 11 2483 13
活用ガイド （ハードウェア編）
(Windows 98) 808-877675-122-A ii iii iv NEC Corporation 1999 v vi PART 1 vii viii PART 2 PART 3 ix x xi xii P A R T 1 2 1 3 4 1 5 6 1 7 8 1 9 10 11 1 12 1 1 2 3 13 1 2 3 14 4 5 1 15 1 1 16 1 17 18 1 19
i
i ii iii iv v vi vii viii ix x xi ( ) 854.3 700.9 10 200 3,126.9 162.3 100.6 18.3 26.5 5.6/s ( ) ( ) 1949 8 12 () () ア イ ウ ) ) () () () () BC () () (
パソコン機能ガイド
PART12 ii iii iv v 1 2 3 4 5 vi vii viii ix P A R T 1 x P A R T 2 xi P A R T 3 xii xiii P A R T 1 2 3 1 4 5 1 6 1 1 2 7 1 2 8 1 9 10 1 11 12 1 13 1 2 3 4 14 1 15 1 2 3 16 4 1 1 2 3 17 18 1 19 20 1 1
パソコン機能ガイド
PART2 iii ii iv v 1 2 3 4 5 vi vii viii ix P A R T 1 x P A R T 2 xi P A R T 3 xii xiii P A R T 1 2 1 3 4 1 5 6 1 2 1 1 2 7 8 9 1 10 1 11 12 1 13 1 2 3 14 4 1 1 2 3 15 16 1 17 1 18 1 1 2 19 20 1 21 1 22
困ったときのQ&A
ii iii iv NEC Corporation 1998 v C O N T E N T S PART 1 vi vii viii ix x xi xii PART 2 xiii PART 3 xiv P A R T 1 3 1 2 PART 3 4 2 1 1 2 4 3 PART 1 4 5 5 6 PART 1 7 8 PART 1 9 1 2 3 1 2 3 10 PART 1 1 2
『戦時経済体制の構想と展開』
1 15 15 17 29 36 45 47 48 53 53 54 58 60 70 88 95 95 98 102 107 116 v 121 121 123 124 129 132 142 160 163 163 168 174 183 193 198 205 205 208 212 218 232 237 237 240 247 251 vi 256 268 273 289 293 311
1... 1 2... 1 1... 1 2... 2 3... 2 4... 4 5... 4 6... 4 7... 22 8... 22 3... 22 1... 22 2... 23 3... 23 4... 24 5... 24 6... 25 7... 31 8... 32 9... 3
3 2620149 3 6 3 2 198812 21/ 198812 21 1 3 4 5 JISJIS X 0208 : 1997 JIS 4 JIS X 0213:2004 http://www.pref.hiroshima.lg.jp/site/monjokan/ 1... 1 2... 1 1... 1 2... 2 3... 2 4... 4 5... 4 6... 4 7... 22
Dual-Luciferase Reporter (DLR TM ) Assay System (a,b,c) DLR TM Assay (Photinus pyralis) (Renilla reniformis ) Luciferase Assay Reagent II (LAR II) Sto
Dual-Luciferase Reporter Assay System No. TM040J 20039 E1910 E1960 I........................................................................ 1 A. Dual-Luciferase Reporter Assay..........................................
長崎県地域防災計画
i ii iii iv v vi vii viii ix - 1 - - 2 - - 3 - - 4 - - 5 - - 6 - - 7 - - 8 - - 9 - 玢 - 10 - - 11 - - 12 - - 13 - - 14 - - 15 - - 16 - - 17 - - 18 - - 19 - - 20 - - 21 - - 22 - - 23 - - 24 - - 25 - -
™…
i 1 1 1 2 3 5 5 6 7 9 10 11 13 13 14 15 15 16 17 18 20 20 20 21 22 ii CONTENTS 23 24 26 27 2 31 31 32 32 33 34 37 37 38 39 39 40 42 42 43 44 45 48 50 51 51 iii 54 57 58 60 60 62 64 64 67 69 70 iv 70 71
1... 1 1... 1 2... 1 3... 1 4... 4 5... 7 6... 7 7... 12 8... 12 9... 13 10... 13 11... 13 12... 14 2... 14 1... 14 2... 16 3... 18 4... 19 5... 19 6.
3 2620149 1 3 8 3 2 198809 1/1 198809 1 1 3 4 5 JISJIS X 0208 : 1997 JIS 4 JIS X 0213:2004 http://www.pref.hiroshima.lg.jp/site/monjokan/ 1... 1 1... 1 2... 1 3... 1 4... 4 5... 7 6... 7 7... 12 8... 12
30% OFF & 31 Eppendorf TC 30% OFF Cell Imaging Dishes 145/170 µm Cell Imaging Coverglasses & Slides 1/2/4/8 chamber > 2 mm 750 µl > 170 µm 145 µ
2016 8 16 10 31 Protect Your Cells from Contamination! 2016 > 30% OFF & 31 > 3 25% OFF info@eppendorf.jp 30% OFF & 31 Eppendorf TC 30% OFF 3 1 8 Cell Imaging Dishes 145/170 µm Cell Imaging Coverglasses
3 A 18 11 19 2 1 JIA 2 (JSCA) 3 4 5 6 7 8 9 10 11 12 13 14 B 4 I. (1) 18 3 29 30 33 12 18 3 542,264 22.5 62 46.3 242 149 29% 3 7 7 1 2 1 2 0.1.1 0.1.2 60 17 1 3 6 8 1 2 0.1.3 1 2 0.1.4 5 (2) (1) 0.2.2
i
14 i ii iii iv v vi 14 13 86 13 12 28 14 16 14 15 31 (1) 13 12 28 20 (2) (3) 2 (4) (5) 14 14 50 48 3 11 11 22 14 15 10 14 20 21 20 (1) 14 (2) 14 4 (3) (4) (5) 12 12 (6) 14 15 5 6 7 8 9 10 7
後期化学_01_濃度
2011 ( ) 1 4 1 100 g g % * 1 100 g 1 g 1 % * 2 (1 g)/(100 g) = 0.01 = 1 % 100 g 100 g 99 g 100 g 1 g 1 % 2.5 g 50 g (2.5 g)/(50 g) = 0.050 = 5.0 % 100 ml g 1 %(w/w) wt% % %(w/v) % / 2 % SI 2 / % 100 ml
untitled
I...1 II...2...2 III...3...3...7 IV...15...15...20 V...23...23...24...25 VI...31...31...32...33...40...47 VII...62...62...67 VIII...70 1 2 3 4 m 3 m 3 m 3 m 3 m 3 m 3 5 6 () 17 18 7 () 17 () 17 8 9 ()
ONLINE_MANUAL
JPN ii iii iv v 6 vi vii viii 1 CHAPTER 1-1 1 2 1-2 1 2 3 4 5 1-3 6 7 1-4 2 CHAPTER 2-1 2-2 2-3 1 2 3 4 5 2-4 6 7 8 2-5 9 10 2-6 11 2-7 1 2 2-8 3 (A) 4 5 6 2-9 1 2-10 2 3 2-11 4 5 2-12 1 2 2-13 3 4 5
ONLINE_MANUAL
JPN ii iii iv v vi 6 vii viii 1 CHAPTER 1-1 1 2 1-2 1 2 3 1-3 4 5 6 7 1-4 2 CHAPTER 2-1 2-2 2-3 1 2 3 4 5 2-4 6 7 8 2-5 9 10 2-6 11 2-7 1 2 2-8 3 (A) 4 5 6 2-9 1 2-10 2 3 2-11 4 5 2-12 1 2 2-13 3 4 5
2
1 2 3 4 5 (written by Dr. ) Stock Solution (one liter) in 4 Buffer A: 0.05 M glucose 20% (1.1 M) glucose 22.7 ml 0.025 M Tris-Cl ph8 1 M Tris (ph8.0) 12.5 ml 0.01 M EDTA 0.5 M EDTA 10 ml Buffer B: 5 M
i ii iii iv v vi vii viii ix x - 1 - - 2 - - 3 - - 4 - - 5 - - 6 - - 7 - - 8 - - 9 - - 10 - - 11 - - 12 - - 13 - - 14 - - 15 - - 16 - - 17 - - 18 - - 19 - - 20 - - 21 - - 22 - - 23 - - 24 - - 25 - -
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 () - 1 - - 2 - - 3 - - 4 - - 5 - 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57
LBP-1810 LIPS機能ガイド
レーザビームプリンタ ＬＩＰＳ機能ガイド ご使用前に必ずこの取扱説明書をお読みください 将来いつでも使用できるように大切に保管してください JPN JPN JPN JPN JPN Copyright 2001 Canon Inc. ALL RIGHTS RESERVED II III IV V VI VII VIII IX X 1 2 3 4 HDD 2 5 6 7 8 9 10 11 12 1
7 i 7 1 2 3 4 5 6 ii 7 8 9 10 11 1 12 13 14 iii.......................................... iv................................................ 21... 1 v 3 6 7 3 vi vii viii ix x xi xii xiii xiv xv 26 27
9 i 9 1 2 3 4 5 6 ii 7 8 9 10 11 12 .......................................... iii ... 1... 1........................................ 9 iv... v 3 8 9 3 vi vii viii ix x xi xii xiii xiv 34 35 22 1 2 1
i ii iii iv v vi vii viii ix x xi xii xiii xiv xv xvi 2 3 4 5 6 7 $ 8 9 10 11 12 13 14 15 16 17 $ $ $ 18 19 $ 20 21 22 23 24 25 26 27 $$ 28 29 30 31 $ $ $ 32 33 34 $ 35 $ 36 $ 37 38 39 40 $ 41 42 43 44
ITR Market View：情報漏洩対策市場2017目次
ITR Market View 2017... 1 1-1... 2 1-2... 3 1-2-1... 3 1-2-2... 6 1-2-3... 6 1-2-4... 6 1-3... 7... 15 2-1... 16 2-1-1... 16 2-1-2... 18 2-1-3... 21 2-2... 22... 23 3-1... 24 3-1-1... 24 3-1-2... 26 3-1-3...
Step2 入門
ii iii iv v vi NEC Corporation 1999 vii C O N T E N T S PART 1 PART 2 PART 3 viii PART 4 ix C O N T E N T S PART 5 x PART 6 xi C O N T E N T S PART 7 xii PART 8 PART 9 xiii C O N T E N T S xiv xv PART
untitled
1998 6 25 ( ) 1 10 1982 10 28 37/7 1990 12 14 45/94 (WHO) 1 1989 12 8 NGO (ECE) 3 1995 10 25 ECE 1991 2 25 1992 3 17 1998 6 4 1 2 1. 2. a b c (a) (b) d 17 3. a b (a) c (b) 4. 5. 3 1. 2. 3. 4. 5. 6. 7.
1.WizardPlusSV日本語ﾌﾟﾛﾄｺｰﾙ
Wizard Plus SV Minipreps DNA Purification System No. TB281J 2001 7 A1270, A1330, A1340, A1460 A1470 Wizard Plus SV Minipreps DNA Purification System (a) DNA 30 20,000bp DNA Recombinant RNasin Ribonuclease
i 1 2 MCB MCB WCB in vitro CAL in vitro CAL In vitro 5 In vivo
EU ICH ICH i 1 2 MCB 2 3 3 MCB WCB in vitro CAL 3 3 3 in vitro CAL 4 4 4 In vitro 5 In vivo 5 5 5 5 6 9 10 10 11 11 11 ii 11 12 12 13 13 13 14 16 17 17 17 19 1 in vivo BSE BSE DNA in vitro in vivo in vivo
ITR Market View：アイデンティティ／アクセス管理市場2018目次
ITR Market View: 2018... 1 1-1... 2 1-2... 3 1-2-1... 3 1-2-2... 5 1-2-3... 5 1-2-4... 5 1-3... 6... 9 2-1... 10 2-1-1... 10 2-1-2... 14 2-2 IDM IAM... 16 2-2-1 IDM IAM... 16 2-2-1-1... 16 2-2-1-2... 19
I
I II III IV V VI VII VIII IX X XI XII XIII XIV XV XVI XVII XVIII XIX XX XXI XXII XXIII XXIV XXV XXVI XXVII XXVIII 1 1. 2 3 2. 4 1 5 6 7 8 9 10 1 2 3 11 3. 12 13 14 1 2 3 15 4 5 16 1 2 3 17 4 18 4. 1 2
ROWCatPathology_J.book
www.dako.jp IHC Solution Visualization Systems Number of Steps 5 4 3 2 1 LSAB TM 2 EnVision TM G 2 Doublestain EnVision TM DuoFLEX Doublestain LSAB TM + EnVision TM G 2 EnVision TM + EnVision TM FLEX 1
AllPrep DNA/RNA Micro プロトコールとトラブルシューティング ( /2008)
July 2007 AllPrep DNA/RNA Micro DNA RNA 5 x 10 5 5 mg Sample & Assay Technologies DNA RNA 3 DNA RNA 11 DNA RNA 19 24 2 AllPrep DNA/RNA Micro 07/2007 DNA RNA RNA AllPrep DNA Spin Column DNA RNeasy MinElute
（報告書まとめ 2004/03/ ）
- i - ii iii iv v vi vii viii ix x xi 1 Shock G( Invention) (Property rule) (Liability rule) Impact flow 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 (
-2-
-1- -2- -3- 1 2 Pseudomonas avenae 34 36 3 25 20 4 1 200 300 2 18 20 5 24 10 ml 20 12 24 3-4- 4 32 33 3 1 60 30 3cm 1 2 1 3 1 3 2 3 1 2 1 1 3 2 2 1 300 3 1 5 0 1 1 2 2 3 3 4-5- 1nl 2n2 3n3 4n4 4N 100 N
262014 3 1 1 6 3 2 198810 2/ 198810 2 1 3 4 http://www.pref.hiroshima.lg.jp/site/monjokan/ 1... 1... 1... 2... 2... 4... 5... 9... 9... 10... 10... 10... 10... 13 2... 13 3... 15... 15... 15... 16 4...
四校＿目次～巻頭言.indd
107 25 1 2016 3 Key Words : A 114 67 58.84 Mann-Whitney 6 1. 2. 3. 4. 5. 6. I. 21 4 B 23 11 1 9 8 7 23456 108 25 1 2016 3 78 9 II. III. IV. 1. 24 4 A 114 2. 24 5 6 3. 4. 5. 3 42 5 16 6 22 5 4 4 4 3 6.
Impress Yourself The new Eppendorf Cell Culture Consumables
Impress Yourself The new Eppendorf Cell Culture Consumables 2 Eppendorf Cell Culture Consumables»Even with 50 years of experience, we started from the beginning.«proven Eppendorf Quality brought to a new
160720_BRO_プロメガ 機器カタログ.indd
2016-2017 INTEGRATED SOLUTIONS page 4 GloMax Discover page 4 GloMax Explorer page 6 GloMax Navigator page 7 GloMax 20/20 page 10 Maxwell RSC page 12 Quantus Fluorometer S/N Cell based assay NanoLuc GloMax
1... 1... 1... 3 2... 4... 4... 4... 4... 4... 6... 10... 11... 15... 30
1 2420128 1 6 3 2 199103 189/1 1991031891 3 4 5 JISJIS X 0208, 1997 1 http://www.pref.hiroshima.lg.jp/site/monjokan/ 1... 1... 1... 3 2... 4... 4... 4... 4... 4... 6... 10... 11... 15... 30 1 3 5 7 6 7
PG1501-PJ7816-CO36363-QuantStudio-3-and-5-brochure-Global-J.indd
Applied Biosystems QuantStudio 3 / QuantStudio 5 PCR PCR Applied Biosystems QuantStudio 3 QuantStudio 5 PCR Thermo Fisher Cloud 1 QuantStudio 3 and 5 Real-Time PCR Systems QuantStudio 3 and 5 Real-Time
1... 1 2... 3 3... 5 1... 5 2... 6 4... 7 1... 7 2... 9 3... 9 6... 9 7... 11 8... 11 5... 7
3 2620149 1 3 6 3 2 198829 198829 19/2 19 2 3 4 5 JISJIS X 0208 : 1997 1 http://www.pref.hiroshima.lg.jp/site/monjokan/ 1... 1 2... 3 3... 5 1... 5 2... 6 4... 7 1... 7 2... 9 3... 9 6... 9 7... 11 8...
44 4 I (1) ( ) (10 15 ) ( 17 ) ( 3 1 ) (2)
(1) I 44 II 45 III 47 IV 52 44 4 I (1) ( ) 1945 8 9 (10 15 ) ( 17 ) ( 3 1 ) (2) 45 II 1 (3) 511 ( 451 1 ) ( ) 365 1 2 512 1 2 365 1 2 363 2 ( ) 3 ( ) ( 451 2 ( 314 1 ) ( 339 1 4 ) 337 2 3 ) 363 (4) 46
I 12 1 26 4 23 42 1 12 2 12 3 12 4 13 5 13 6 13 7 13 8 14 9 14 10 14 11 14 2 26 3 26 10 27 28 11 28 12 28 13 28 VI 29 1 29 1 29 34 5 35 6 35 7 35 8 35 9 36 10 36 11 36 12 36 1 42 2 42 24 43 25 43 26 43
能書単頁9[1].5(2)
![能書単頁9[1].5(2)](/thumbs/87/96620151.jpg "能書単頁9[1].5(2)")
ips CytoTune -ips ver.1.0 1 I CytoTune ips 3 II 3 3 4 5 III CytoTune ips ips 6 6 6 6 7 1) 2) ips 8 ips 8 SeV 9 SeV 10 IV Q&A 11 V 12 12 VI...13 13...13 2 I CytoTune ips CytoTune ips 4 OCT3/4SOX2 KLF4c
i ii i iii iv 1 3 3 10 14 17 17 18 22 23 28 29 31 36 37 39 40 43 48 59 70 75 75 77 90 95 102 107 109 110 118 125 128 130 132 134 48 43 43 51 52 61 61 64 62 124 70 58 3 10 17 29 78 82 85 102 95 109 iii
178 5 I 1 ( ) ( ) 10 3 13 3 1 8891 8 3023 6317 ( 10 1914 7152 ) 16 5 1 ( ) 6 13 3 13 3 8575 3896 8 1715 779 6 (1) 2 7 4 ( 2 ) 13 11 26 12 21 14 11 21
I 178 II 180 III ( ) 181 IV 183 V 185 VI 186 178 5 I 1 ( ) ( ) 10 3 13 3 1 8891 8 3023 6317 ( 10 1914 7152 ) 16 5 1 ( ) 6 13 3 13 3 8575 3896 8 1715 779 6 (1) 2 7 4 ( 2 ) 13 11 26 12 21 14 11 21 4 10 (
VB-C50i/VB-C50iR 使用説明書
a ii iii iv a v vi vii viii d a a d ix a a d b a a a b x a a g a g a e a a xi a a a xii a a xiii xiv 1-2 1-3 d 1-4 1-5 1-6 1-7 1-8 1-9 1-10 1-11 1-12 2-2 2-3 a 2-4 a 2-5 a 2-6 2-7 2-8 2-9 2-10 2-11 2-12
呼吸鎖複合体酵素活性測定 酵素活性の測定には SPECTROstar Nano (BMG LABTECH) を用い 全ての測定は 100 μl の反応系で行う マルチウェルプレートは 96 well, half area UV-Star Microplate (Greiner Bio One) を用
呼吸鎖複合体酵素活性測定 酵素活性の測定には SPECTROstar Nano (BMG LABTECH) を用い 全ての測定は 100 μl の反応系で行う マルチウェルプレートは 96 well, half area UV-Star Microplate (Greiner Bio One) を用いる サンプルはヒトの組織 ( 大動脈中膜平滑筋層 心筋 脂肪組織 ) から調製した画分を用いる 画分は膜を破砕することで活性測定試薬を透過させるために
2620149 3 8 2 198802 492/ 198802 492 1 4 5 JISJIS X 0208 : 1997 JIS JIS X 0213:2004 http://www.pref.hiroshima.lg.jp/site/monjokan/ 1... 1 1... 1 2... 2 3... 2 4... 2 5... 3 6... 3 7... 4 8... 4 2... 4
OFISTAR H7000ファクス編（2版 ）
i ii A iii A A A A A A A A A B C D A A B C D iv B A AB A A B A B A v A C B A ABC A B C A A vi A B B A A A A A A vii A A viii ix 4 5 6 8 9 0 x xi 5 6 9 6 06 C 4 5 8 6 9 0 C 06 06 06 064 065 066 06 068 069
かんたん操作ガイド
1-2 i ii iii iv v vi vii viii 1Chapter 1-1 1-2 1-3 1-4 1-5 1-6 1-7 1 3 2 4 1-8 1-9 1-10 2Chapter 2-1 2-2 Check! 2-3 Point 2-4 Point 2-5 Point 2-6 1 a a b c 2 a b c b 2-7 c 5 3 4 6 a 2-8 b b c b 7 a c a
...J......1803.QX
5 7 9 11 13 15 17 19 21 23 45-1111 48-2314 1 I II 100,000 80,000 60,000 40,000 20,000 0 272,437 80,348 82,207 81,393 82,293 83,696 84,028 82,232 248,983 80,411 4,615 4,757 248,434 248,688 76,708 6,299
Adobe Acrobat DC 製品比較表
X X Adobe, the Adobe logo, Acrobat, the Adobe PDF logo, Creative Cloud, and Reader are either registered trademarks or trademarks of Adobe Systems Incorporated in the United States and/or other countries.
1981 i ii ➀ ➁ 61
(autism) 1943 60 1981 i ii ➀ ➁ 61 DSM- 4 3 29 99 DSM- 62 1 2 3 4 4 vi 63 64 ix x xi 204 3 65 176 90 3 79 66 DSM- 82 67 68 ➀ ➁ ➂ 69 34 5 70 JR 71 i 1944 ii iii 28 72 iv 48 v ➀ vi PHP 39 vii 176 viii ➄ 77
