ROWCatPathology_J.book

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1 IHC Solution Visualization Systems Number of Steps LSAB TM 2 EnVision TM G 2 Doublestain EnVision TM DuoFLEX Doublestain LSAB TM + EnVision TM G 2 EnVision TM + EnVision TM FLEX Comparative Features of Dako Visualization Systems Sensitivity EnVision TM FLEX+ Product Name No. of Steps Primary Antibody Primary Antibody Enzyme Label Technology EnVision TM FLEX 2 Concentrate or FLEX RTU Mouse + Rabbit HRP Dextran (Biotin-Free) EnVision TM FLEX+ 3 Concentrate or FLEX RTU Mouse + Rabbit HRP Dextran (Biotin-Free) EnVision TM + 2 Concentrate Mouse and/or Rabbit HRP Dextran (Biotin-Free) EnVision TM DuoFLEX Doublestain CSA II 4 Cocktail or Concentrate Mouse + Rabbit AP and HRP Dextran (Biotin-Free) EnVision TM G 2 3 Concentrate or RTU Mouse + Rabbit AP Dextran (Biotin-Free) EnVision TM G 2 Doublestain 5 Concentrate or RTU Mouse + Rabbit AP and HRP Dextran (Biotin-Free) CSA 5 Concentrate Mouse or Rabbit HRP Tyramide CSA II 4 Concentrate Mouse or Rabbit HRP Tyramide (Biotin-Free) LSAB TM + 3 Concentrate Mouse + Rabbit + Goat CSA AP or HRP Labeled Streptavidin-Biotin LSAB TM 2 3 Concentrate or RTU Mouse + Rabbit AP or HRP Labeled Streptavidin-Biotin 106 : (9) : :

2 EnVision FLEX EnVision TM FLEX, High ph Link Envision FLEX High ph AutostainerLink D K8000 HRP tests EnVision TM FLEX Mini Kit, High ph Link Envision FLEX Mini Kit High ph AutostainerLink D K8023 HRP tests EnVision TM FLEX Dako EnVision TM FLEX EnVision TM FLEX FLEX EnVision TM FLEX, High ph Dako Autostainer/Autostainer Plus Envision FLEX High ph Autostainer/Autostainer plus D K8010 HRP tests EnVision TM FLEX Mini Kit, High ph Dako Autostainer/Autostainer Plus Envision FLEX Mini Kit High ph Autostainer / Autostainer plus D K8024 HRP tests Visualization Systems IHC Solution K8000 K8010 K8023 K x 40 ml 10 x 13 ml 1 x 40 ml 3 x 13 ml 3 x 40 ml 10 x 13 ml 1 x 40 ml 3 x 13 ml 3 x 3 ml 3 x 3 ml 1 x 3 ml 1 x 3 ml 12 x 20 ml 20 x 13 ml 5 x 20 ml 7 x 13 ml 9 x 30 ml 9 x 30 ml 3 x 30 ml 3 x 30 ml 4 x 1 L 4 x 1 L 2 x 1 L 2 x 1 L TEL : FAX :

3 EnVision FLEX Envision FLEX Antibody Diluent Envision FLEX K tests, 120 ml Rabbit LINKER Envision FLEX+AutostainerLink K tests, 40 ml IHC Solution Visualization Systems Hematoxylin Link Envision FLEX AutostainerLink K8008 AutostainerLink tests, 3 x 45 ml Hematoxylin Dako Autostainer/Autostainer Plus Envision FLEX Autostainer / Autostainer plus K tests, 10 x 13 ml Mouse LINKER Envision FLEX+ AutostainerLink K tests, 40 ml Mouse LINKER Envision FLEX+ Autostainer / Autostainer plus K tests, 3 x 13 ml Rabbit LINKER Envision FLEX+Autostainer / Autostainer plus K tests, 3 x 13 ml Target Retrieval Solution, High ph Envision FLEX High ph 50 x K x 30 ml Target Retrieval Solution, Low ph Envision FLEX Low ph 50 x K x 30 ml Wash Buffer Envision FLEX 20 x K L 108 : (9) : :

4 EnVision TM DuoFLEX 2 mouse STEP 1 Application of mouse and rabbit primary antibodies. rabbit mouse rabbit STEP 2 Application of HRP-conjugated polymers and secondary polymers. EnVision TM DuoFLEX Doublestain System EnVision TM DuoFLEX SK110 HRP/AP, Rabbit/Mouse tests, 30 ml K6807 HRP/AP, Rabbit/Mouse tests, 30 ml EnVision TM DuoFLEX Autostainer Link (SK110) Autostainer K6807 DAB+ (HRP) AP) EnVision TM DuoFLEX Duo FLEX 1 200µL 300µL mouse rabbit STEP 3 Application of AP-conjugated polymers. DAB mouse DAB LPR rabbit LPR STEP 4 Application of chromogenic substrates: brown DAB and LPR. Visualization Systems IHC Solution TEL : FAX :

5 EnVision 2 IHC Solution Visualization Systems Step 1. Step 2.EnVision TM EnVision TM G 2 Doublestain System Rabbit/Mouse DAB+/Permanent Red EnVision G 2 DAB+/ K tests Vial 1: 11mL x 3 Vial 2: 11mL x 3 Vial 3: DAB+ 11mL x 3 Vial 4: DAB+ 11mL x 3 Vial 5: 11mL x 3 Vial 6: 11mL x 3 Vial 7: 11mL x 3 Vial 8: 11mL x 3 Vial 9: 300L x 1 EnVision TM G 2 System/AP Rabbit/Mouse Permanent Red EnVision G 2 / AP K tests/500 tests EnVision TM + Kits EnVision TM + System / HRP, Mouse (DAB+), RUO K tests K tests ABC K4006 K ml x ml x 1 15 ml x ml x 1 18 ml x ml x 1 3,3'- DAB 1 ml x 1 5 ml x 1 DAB+ K ml 3,3'- K ml EnVision TM + Dual Link, Single Reagents EnVision TM + K4063 HRP 150 tests, 15 ml K4061 HRP - Autostainer 1100 tests, 10 x 11 ml ENVISION Autostainer 10 11mL 15mL 2 K4063 K ml 11 ml x : (9) : :

6 EnVision TM + Single Reagents EnVision TM + / HRP, RUO K tests, 15 ml K tests, 110 ml K tests, 15 ml K tests, 110 ml 2 K4000 K ml 110 ml K4002 K ml 110 ml EnVision TM Detection System EnVision TM Detection System / HRP, Rabbit/Mouse (DAB+), RUO K tests Autostainer/Autostainer plus DAB K5007 : 100 ml A : 100 ml x 1 B : 250 ml x 1 C : 3,3'- DAB 5 ml x 1 Visualization Systems IHC Solution TEL : FAX :

7 CSA LSAB sabc CSA System IHC Solution Visualization Systems 112 Step 1. Step 2. Step 3. - CSA, Catalyzed Signal Amplification System* CSA HRP DAB K1500, 150 tests ABC Step 1. Step 2. CSA II, Biotin-Free Catalyzed Amplification System* CSA II K tests, 15 ml CSA II FITC FITC DAB LSAB ABC CSA II CSA Code No. K1500 : (9) : : Step 4. Step 5. K ml x ml x 1 15 ml x 1 A 1 ml x 1 B 1 ml x 1 PBS 25 ml x 1 15 ml x 1 15 ml x 1 DAB 10 x 1 6 ml x ml x 1 x 1 x 1 x 1 Step 3.FITC Step 4. FITC K ml x ml x 1 15 ml x 1 FITC 15 ml x 1 FITC 15 ml x 1 18 ml x 1 3,3 1 ml x 1 CSA II Rabbit Link CSA Rabbit Link K tests, 15 ml CSA II Rabbit Link CSA II Code No. K1497 CSA II Code No. K1497

8 LSAB TM Kits Step 1. Step 2. Step 3. LSAB TM + Kits, Universal LSAB TM + K0678 AP K0679 HRP K0690 HRP 150 tests 150 tests 1100 tests HRP K0679 K ml x 1 15 ml x ml x 1 15 ml x ml x 1 18 ml x 1 3, 3'- 1 ml x 1 AP K ml x 1 15 ml x 1 2 ml x 1 2 ml x 1 30 ml x 1 x 1 1 ml x 1 LSAB TM 2 Kits, Universal LSAB TM 2 Kits, Universal / HRP, Rabbit/Mouse, RUO K0675 HRP 1100 tests K0609 HRP 150 tests K0675 LSAB TM K0609 K mL x 1 110mL x 1 K ml x 1 Bovine 15 ml x 1 Streptavidin Streptavidcin, RUO P ml Streptomyces avidinii P0397 1:300 1:1,000 in situ 1:300 1:1,000 W.Blot. 1:3,000 1:9,000 ELISA 1:1,000 1:15,000 Visualization Systems IHC Solution TEL : FAX :

9 IHC IHC IHC Solution Ancillaries for IHC in situ hybridization AEC Substrate-Chromogen AEC Substrate-Chromogen, Ready-to-use, RUO K tests, 110 ml in situ hybridization Code No. C0563 Ultramount Mounting Medium Code No. S1964 K3464 AEC 0.75mg/mL mL or 110mL * 2 8 AEC+ Substrate-Chromogen AEC K tests, 15 ml K tests, 110 ml in situ hybridization AEC Code No. C0563 Ultramount Mounting Medium Code No. S1964 K3461 AEC 15mL 1 3-Amino-9-Ethyl Carbazole, K3469 AEC 110mL 1 3-Amino-9-Ethyl Carbazole, CSA System Code No. K1500 * BCIP/NBT Substrate System BCIP/NBT K tests in situ Hybridization [ ] BCIP/NBT 15mL * BCIP/NBT * BCIP/NBT 0.1 1mmol/L Code No. X3021 DAB+, Liquid DAB+, Liquid, RUO K tests, 15 ml K tests, 110 ml in situ hybridization DAB K3467 K3468 DAB 3,3'-Diaminobenzidine Tetrahydrochloride 1mLx1 5mLx1 /ph ml x 1 110mLx1 1mL DAB 1 20µL 2 8 CSA System Code No. K : (9) : :

10 IHC Fuchsin+ Substrate-Chromogen K tests, 30 ml/1100 tests, 110 ml in situ hybridization Code No.K mL 30mL mL 2mL 7mL 2mL 110mL 30mL mL 3 2 2mL Levamisole Code No. X New Fuchsin Substrate System K tests, in-situ hybridization,, Code No. X mol/L ph8.2 7mL 5mL 0.5% 5mL 5mL mol/L ph8.2 2mL % , 2 a+b 3 4 2mL Levamisole Code No. X ,, Ancillaries for IHC IHC Solution Permanent Red Substrate-Chromogen, Liquid K tests, 30 ml/1100 tests, 110 ml in situ hybridization Texas Red 110mL 30mL mL 1mL 110mL 30mL 30 3mL mL 1 3. TEL : FAX :

11 IHC IHC Solution Ancillaries for IHC Antibody Diluent S ml/125 ml ph, ph,, 15mmol/L 50mL, 125mL Antibody Diluent, Dako REAL TM Dako REAL TM S ml Antibody Diluent, Background Reducing S3022, Tween 50 ml/125 ml ph, ph,,, 0.1% Tween, 15mmol/L Tween Biotin-Blocking System X ml + 15 ml - LSAB LAB sabc ABC,, * 2 0.3mol/L 263mg 15mmol/L 0.1% 15mL 15mmol/L 0.01% 15mL * 2 8 * 0.3mol/L 263mg STEP 1 :, 10 TBS PBS TBS PBS 5 STEP 2 :, 10 TBS PBS TBS PBS 5 Levamisole Solution X ml in situ hybridization Marginal Zone B Cell 1mL * 2 8 Peroxidase-Blocking Solution, Dako REAL TM Dako REAL TM S ml 116 : (9) : :

12 IHC Phosphate-Buffered Saline, ph mol/L NaCl 20mmol/L ph7.0 S x 1 L, in situ hybridization NaH 2 PO 4, Na 2 HPO 4, 6 * 1 1L * 0.02mol/L, 0.15mol/L, ph 7.0 * 2 8 Protein Block, Serum-Free X0909, 110 ml,,,, 0.25%,, 15mmol/L * 2 8 X Target Retrieval Solution Target Retrieval Solution S ml, 10x concentrated S ml, in-situ hybridization DNA RNA ph * S S1700 * 2 8 Target Retrieval Solution, ph 9 Target Retrieval Solution ph 9 S ml, 10x concentrated S ml 10mmol/L, ph6.0 ph 1mmol/L EDTA 10mmol/L, ph 9.0 TE Target Retrieval Solution, ph 9.0 Code No. S2367/S2368 TE S S2368 Target Retrieval Solution, ph 9 10x, 3-in-1 Target Retrieval Solution ph 9, 10x, 3-in-1 S ml, 10x concentrated Dako Target Retrieval Solution, ph 9, 10x, 3-in-1 Autostainer Link Dako PT Link Code No. PT100 1 Tris-Buffered NaCl Solution with Tween 20, ph %Tween mol/L NaCl 0.05mol/L ph7.6 10x S ml, 10x concentrated, in situ hybridization CSA System Code No. K1500 GenPoint TM System Code No. K0620 Tween * 2 8 * 0.05mol/L, 0.3mol/L, Tween %, ph 7.6 Ancillaries for IHC IHC Solution Target Retrieval Solution, Citrate ph 6 REAL TM S ml, 10x concentrated ph TEL : FAX :

13 IHC IHC Solution Ancillaries for IHC Tris-Buffered Saline, ph mol/L NaCl 0.05mol/L ph7.6 S x 1 L S x 5 L, in situ hybridization S3001,, 6 S1968,, 2 S L S L * 0.05mol/L, 0.15mol/L, ph 7.6 * 2 8 Tween 20 Tween20 Autostainer S1966 Autostainer 100 ml Autostainer Tween 20 Code No. S3006 Tween 20 Wash Buffer 10x 0.05% Tween mol/L NaCl 0.05mol/L ph7.6 10x S L, 10x concentrated 118 : (9) : :

14 IHC Dako REAL TM Hematoxylin Dako REAL TM S ml Methyl Green S ml Fluorescence Mounting Medium S ml Glycergel Mounting Medium, Aqueous C ml * Ultramount Permanent Mounting Medium, Aqueous S ml DAB AEC Fast Red /overnight Ancillaries for IHC IHC Solution TEL : FAX :

15 IHC in situ hybridization IHC Solution Ancillaries for IHC Pepsin S x 250 to 500 ml, in situ hybridization 1 500mL 0.2mol/L in situ hybridization 1 250mL 0.2mol/L * , in situ hybridization Dako Pen S unit, " ", Silanized Slides S slides, in-situ hybridization Triethoxyaminopropylsilane,, * Hydrophilic Silanized Slides Proteinase K K S3004 2mL S tests, 15 ml/1100 tests, 110 ml, in situ hybridization 20mg/mL K * K 2 8 K 0.4mg/mL K 40µL 0.05mol/L ph mL in situ hybridization K 0.8mg/mL K 80µL 0.05mol/L ph mL * K 20 2mL K 6 15, in situ hybridization S mg/mL 6 15 in situ hybridization Hydrophilic Silanized Slide S slides 120 : (9) : :

Universal Label Printer Link DL412 1 unit Autostainer Link, Artisan Link 3,000 100-240V, 50-60Hz 2.

16 Universal Label Printer Link DL412 1 unit Autostainer Link, Artisan Link 3, V, 50-60Hz 2.1kg 190mm x 260mm x 190mm Labeling Systems IHC Solution Autostainer Link Artisan Link Slide Label Kit, Large Flap S labels Autostainer Link 1 3,000 Slide Label Kit, Small Flap DL labels Artisan Link 1 1,500 TEL : FAX :

Microsoft Word ab205921_自動染色プロトコール_YU_IS.docx

Microsoft Word ab205921_自動染色プロトコール_YU_IS.docx 抗 PD-L1 抗体 [clone 28-8] 動免疫組織染装置別プロトコール PD-L1 RabMAb [clone 28-8] (ab205921) BioGenex 社 i6000( オフライン抗原賦活化 ) Leica 社 BOND RX( オンライン抗原賦活化 ) Ventana 社 Ultra( オフライン抗原賦活化 ) Dako 社 Omnis BioGenex 社 i6000 プロトコール