Amnis TM CellStream TM CellStream TM

Size: px

Start display at page:

Download "Amnis TM CellStream TM CellStream TM"

あおいひろもり
4 years ago
Views:

1 Amnis TM CellStream TM CellStream TM

2 Amnis TM CellStream TM Amnis TM CCD FITC PE CFR Part FDA

3 TM CellStream Luminex Time Delay Integration TDI CellStream CCD CellStream Amnis CCD

CellStream TM 8 Spherotech CellStream 8 MESF Molecules of Equivalent Soluble Fluorochrome MESF < FITC MESF <5 PE CellStream 56/5 58/6 58/ 6/ 7/87 77/56 75 nm.8.5..9.6. e e e5 e6 56/5.8.5..9.6. e e e5 e6 58/6.

4 CellStream TM 8 Spherotech CellStream 8 MESF Molecules of Equivalent Soluble Fluorochrome MESF < FITC MESF <5 PE CellStream 56/5 58/6 58/ 6/ 7/87 77/56 75 nm e e e5 e6 56/ e e e5 e6 58/6.5.5 e e e5 58/ 5 nm CellStream 88 nm e e e5 e6 56/ e e e5 e6 58/6 e e e5 e6 58/6 e e e5 e6 58/ e e e5 e6 58/ e e e5 e6 6/ e e e5 e6 6/ e e e5 e6 7/87 e e e5 e6 7/ e e e5 77/ nm e e e5 e6 58/ e e e5 e6 6/ e e e5 e6 7/87 e e e5 e6 77/56 6 nm e e e5 e6 7/ e e e5 77/56 CellStream. m 5 9 nm Megamix-Plus FSC 7 SSC FCS 88nm Side Scatter (SSC) e+5 e+ e+ e+ e+ Forward Scatter (FSC)

EVEV CellStream EV PE-Anti-CD5 APC-CD PBS PE-Anti-CD5 /APC-CD EV Triton X- TX SSC FSC EV B PE + C APC + L PE + APC + D L B C PE + APC + EV +Triton X- EV +Triton X- A Population: All B Population:

5 EVEV CellStream EV PE-Anti-CD5 APC-CD PBS PE-Anti-CD5 /APC-CD EV Triton X- TX SSC FSC EV B PE + C APC + L PE + APC + D L B C PE + APC + EV +Triton X- EV +Triton X- A Population: All B Population: Potential EVs C Population: Potential EVs.8.8 SSC Frequency.6. PE+ Frequency.6. APC+ Potential EVs FSC e+ PE Intensity e+ e+ APC Intensity e+ D A. PE+ Objects per μl B. APC+ Objects per μl 5 5 Average objects/μl Average objects/μl 5 5 :6 : : :8 :96 :6 : : :8 :96 Dilution Dilution EV Antibody only Antibody + TX EV + TX Buffer only EV Antibody only Antibody + TX EV + TX Buffer only

6 CellStream TM 6 CellStream 6 PBMC i. PBMC 6 A. B. C. D. E. F. G. H. ii. PBMC T A. B. C. D. E. F. 5

7 5 L* PBMC 6L 5 Live/Dead Violet N/A Viability CD BUV96 SK CD T Cells CD56 APC-R7 5.H NKs HLADR APCCy7 L DCs 5 CD BV 6H6 pdcs 6 CD V5 L7 B Cells 7 CD8 BV57 RPA-T8 CD8 T Cells 8 CD5 BV65 BC96 Treg 9 CD6 BV65 G8 Monocytes CD BV785 M5E Monocytes CD5RA AF88 HI Naïve/memory CD8 PE HIT Activation CD BB7 HITa T cells CCR7 PECF59 55 Central/effector 5 CDc PeCy5.9 mdcs 6 CD7 PeCy7 A9D5 Treg.5 FBS mmedta PBS CellStream 7 *

CellStream TM 96 88 nm 7 CellStream TM CellStream 56/5 58/6 58/ 6/ 7/87 77/56 75 nm AF5 BUV95 BUV96 BUV56 QDot65 BUV66 BUV85 5 nm BV Cascade Blue BV5 QDot565 BV65 BV65 BV786

8 CellStream TM nm 7 CellStream TM CellStream 56/5 58/6 58/ 6/ 7/87 77/56 75 nm AF5 BUV95 BUV96 BUV56 QDot65 BUV66 BUV85 5 nm BV Cascade Blue BV5 QDot565 BV65 BV65 BV786 CellStream 88 nm 5 nm 56 nm FITC AF88 PE PE PE PE-TR mcherry PE-TR mcherry PE-TR PE-Cy5.5 PE-Cy5.5 PE-Cy5.5 PE-Cy7 PE-Cy7 PE-Cy7 6 nm APC Cy5 APC-Cy7 APC-AF75 7 nm Cy7 CF75 CellStream 7 7

9 CFR Part 96 / / PDF 8

10 ダブレット除去 Aspect ratio Aspect Ratio e+ e+ 6e+ 8e+ e+5 FSC 9

11 CellStream Luminex サービスプランの利点 * 機能 MESF < FITC MESF <5 PE CV* (precision) <% ( fluorescent, plus FSC, SSC) nm (area; aspect ratio; raw max. pixel), cells/second.66 L/min (Low speed/high sensitivity).6 L/min (High speed) (W D H) FSC < nm from 5 nm SSC < nm from 785 nm 7 decades mm 96 *

12 CellStream TM Base System with 88 nm Laser ( mw) and Autosampler CellStream TM Option 75 nm Laser, 7 mw CellStream TM Option 5 nm Laser, 75 mw CellStream TM Option 5 nm Laser, 5 mw CellStream TM Option 56 nm Laser, 5 mw CellStream TM Option 6 nm Laser, 5 mw CellStream TM Option 7 nm Laser, mw CellStream TM Software Multi Access CellStream TM Calibration Reagent CellStream TM On-site Training CellStream TM Installation CellStream TM IQOQ Document CS-96 CS-75 CS-5 CS-5 CS-56 CS-6 CS-7 CS- CS- CS-5 CS-6 CS-65 ルミネックスジャパン株式会社 6- 東京都港区麻布台 -7- 神谷町麻布台ビルテクニカルサポートお問い合わせ窓口 Tel: ( 受付時間 9: 8: 土日祝祭日年末年始を除く ) supportjapan@luminexcorp.com 9 Luminex Corporation. All rights reserved. Amnis CellStreamTriton BR6855_AP-R-JP

MACS機器予算申請カタログ

MACS機器予算申請カタログ MACS Instruments MACSQuant automacs MultiMACS gentlemacs MACSmix TM MACSQuant 3 MACSQuant Analyzer 10 MACSQuant VYBMACSQuant X MACSQuant X Coming soon! 384 well plate Laser Channel Filter (nm) Dye or Parameter