免疫学研究のための製品群
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- れんま おおかわち
- 6 years ago
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1 ver.1.4 The path to new discoveries From basic to clinical research in immunology Good research starts with happy cells.
2 gentlemacs TM Octo Dissociator with Heaters TM Whole Blood MicroBeads 37 RNA Option 0.25mL 90-95% automacs Pro Separator Whole Blood 40 Tissue Storage Solution # Dissociation Kit MACS gentlemacs Dissociator Lamina Propria Dissociation Kit & gentlemacs Dissociator P4P8WEB MACSxpress Isolation Kit ml 8 ml 5 min (15 min) MACSxpress Separator PI CD45 + cells Live cells Live cells SSC Figure 1 C57BL/6Lamina Propria Dissociation Kit (gentlemacs Dissociator Propidium Iodide Solution ( ) MACSQuant Analyzer %viability FSC TCRβ-APC-Cy7 CD45R(B220)-VioBlue MACS Tissue Storage Solution Adipose Tissue Dissociation Kit () Epidermis Dissociation Kit () Epidermis Dissociation Kit () Lamina Propria Dissociation Kit () Liver Dissociation Kit () Lung Dissociation Kit () Spleen Dissociation Kit () Tumor Dissociation Kit () Tumor Dissociation Kit () Whole Skin Dissociation Kit () CD11c-VioBlue CD11b-APC-Vio770 CD15-PE MACSxpress Neutrophil Isolation Kit A B C (%) (%) CD16-APC Figure 2 (A) (CD15 + CD16 + )MACSQuant Analyzer(B) 6 982%)9523%)981%)1 ml (C) MACSxpress Neutrophil () MACSxpress Starting Kit MACSxpress 2
3 2MACS25 MACS MACS Technology MACS Technology A B C S N S N 50 nm a b MACS Technology 1 50 nm) Figure 4 MACS (A) MACS (B) (C) (a) (b)20 MACS Technology Truly Untouched Cells S N S N Figure 3 PBMCCD14/CD16(red) (green)amacs B MACS Technology viability automacs MS Column LS Column LD Column Deplete Deplete_S Deplete_05 Deplete_025 1 Target 10 6 cells in 10 8 cells Flow Sorting Total MACS & Flow Sorting Total automacs Pro 5 & 40 1 ~10 5 cells MiniMACS Separator automacs Pro Separator Buffy coatleucopac MultiMACS Cell24 Separator Plus MultiMACS X 3
4 MACS at a Glance & NK cells Human PBMCs MACSprep PBMC # CD4 + CD4 # Whole Blood CD4 # CD4 + T Cell # MACSxpress CD4 T Cell # Treg cells CD3 # Whole Blood CD3 # CD4 + CD25 + Regulatory T Cell # CD4 + CD25 + CD127 dim/ Regulatory T Cell # CD25 + CD49d Regulatory T Cell # CD4 + CD25 + CD45RA + Regulatory T Cell # MACSxpress Treg # CD4 + T cell subsets Naive Naive Pan T Cell # REAlease CD3 # CD8 + CD2 # Pan T Cell # CD8 # Whole Blood CD8 # CD8 + T Cell # MACSxpress CD8 T Cell # γ/δ + Anti-TCRγ/δ # TCRγ/δ + T Cell # DN Double-negative T Cell # CD8 + T cell subsets CD6 # MACSxpress Pan T Cell # NK CD3 + CD56 + NKT Cell # Anti-iNKT # ILC2 ILC2 # NK cells CD56 # Whole Blood CD56 # NK Cell # MACSxpress NK Cell # NK cell subsets CD56 + CD16 NK Cell # CD56 + CD16 + NK Cell # CD56 + CD8 + /CD8 NK Cell # CD56 + CD57 + NK Cell # CD57 # CD4 + RTE CD4 + Recent Thymic Emigrant # Naive CD4 + Naive CD4 + T Cell # Skin-homing CD4 + CLA + T Cell # Anti-CLA # Naive CD8 + Naive CD8 + T Cell # Memory CD8 + CD8 + Memory T Cell # Other Memory CD4 + Memory CD4 + T Cell # MACSxpress CD4 Memory T Cell # TEM cells CD4 + Effector Memory T Cell # TCM cells CD4 + Central Memory T Cell # TEMRA cells CD8 + CD45RA + Effector T Cell # Cytotoxic CD8 + CD57 + T Cell # NK : NK Cell # REAlease CD56 # TH1 cells IFN-γ Secretion Assay # TH2 cells CD294 (CRTH2) # IL-4 Secretion Assay # TH17 cells IL-17 Secretion Assay # Effector CD27 # Activated T cell subsets Other CD25 # CD30 # CD69 # CD154 # CD137 # CD8 + T CD8 + T Cell # REAlease CD8 # CD4 + T CD4 + T Cell # REAlease CD4 # CD28 # CD62L # CD45RA # CD45RO # CD127 #
5 Mouse NK cells CD3ε # Pan T Cell # CD90.1 # CD90.2 # CD5 (Ly-1) # CD4/CD8 (TIL) # CD49b (DX5) # NK Cell # Anti-NKp46 # CD4 + CD4 (L3T4) # CD4 + T Cell # CD4 (TIL) # Treg cells CD4 + CD25 + Regulatory T Cell # CD25 # CD8 + CD8a (Ly-2) # CD8a + T Cell # CD8 (TIL) # γ/δ + TCRγ/δ + T Cell # ink NK1.1 + inkt Cell # T cell subsets MACS () Naive CD4 + Naive CD4 + T Cell # CD4 + CD62L + T Cell # Memory : CD4 + or CD8 + T Cell CD62L (L-selectin) # Naive CD8 + Naive CD8a + T Cell # Activated CD25 # CD154 # untouched 2 TH1 cells Mouse IFN-γ Secretion Assay # TH17 cells Mouse IL-17 Secretion Assay # TH2 cells Mouse IL-4 Secretion Assay # MACSxpress MACSxpressMACS MACSxpress2 MACS Cytokine Secretion Assays : + CD45Catch Reagent37 C Catch Reagent Anti-PE 5
6 & NK cells PBMC Pan T, CD4 + T, CD8 + T, NK (Untouched) 18 (PBMC) CD56 () (CD56 + ) CD4 + T Cell () (PBMC) (CD3 + CD4 + ) min fast kit! CD56-PE CD56-PE CD3-FITC CD3-FITC CD3-FITC CD3-FITC Figure 7 CD56 + NK, NKT CD4-PE CD4-PE Figure 5 CD4 + T CD4 + T Cell () () (CD3ε + CD4 + ) CD4-APC CD4-APC CD3ε-PE CD3ε-PE min fast kit! Research diversity: & & T 2 CD45RA-FITC Naive CD4 + T Cell (PBMC) CD45RA-FITC (CD4 + CD45RA + ) min fast kit! Figure 6 CD4 + T MACSQuant Analyzer BALB/cC57BL/6 CD3ε + CD4 + 95% CD11cAnti-MHC Anti-TCRγ/δ CD4-APC CD4-APC Figure 8 CD4 + T >96%CD4 + MACSTNK Naive CD4 + T Cell () (CD4 + CD62L + CD44 -/low ) min fast kit! RA T T T NK In vitro polarization Th T GvHD GvHD HIV HIV CD3ε-APC-Vio770 CD4-VioBlue CD3ε-APC-Vio770 CD4-VioBlue Figure 9 CD4 + T MACSQuant AnalyzerBALB/cC57BL/6CD3ε + CD4 + CD62L + 95%CD4 + CD25 + regulatory T(<0.3% CD44-FITC CD62L-PE 6
7 T cell subsets of your choice T Progressive regulatory T cell research: T T (Tregs) FoxP3 expansion CD4 + CD25 + Regulatory T Cell Isolation Kit ( ) CD4 + CD25 + Regulatory T Cell Isolation Kit ( ) PBMCCD4 + TCD25 + / CD25 - Tregs Responder TCD4 + CD25 - Tregs CD4 + CD25 + CD127 dim/- Regulatory T Cell Isolation Kit ( ) CD127 dim/- FoxP3Tregs In vitro expansion CD4-FITC CD4 + CD25 + CD127 dim/- T CD25-APC Figure 10 T MACSxpress Treg Isolation Kit ( ) T MACSxpress30 MACSxpress Treg CD25-APC gated on CD4 + cells CD127-PE Handling time (min) Anti-FoxP3-APC gated on CD4 + cells CD127-PE Competitor Kit A CD4 + CD25 + Regulatory T Cell PBMC Competitor Kit B MHC multimer 2 CD154-APC (PBMC) Anti-IL-17-PE IL-17 CD154-APC Anti-IL-17-PE Figure 12 IL-17 PBMCCytoStim (# ) CD40CD40 IL-17 CD40 (# ) CD154CD4 + TCD154 + IL %90% TH1 (IFN-γ), TH2 (IL-4), TH17 (IL-17), IL-10 secreting Tr1 cytotoxic T TH17TH1 EAE Tr1 Tr1 Tr1 T gated on CD4 + cells MACST MHC multimer-peanti-pe (# ) CD154 CD154 () (# ) CD154 () (# ) Aspergillus-reactive CD4 + T CD154-APC Anti-IL-17-PE T Treg detection Kit (PE/APC)( ) Treg detection Kit (PE/APC)( ) CD4 CD154 CD154 FoxP3, CD4, CD25FcR CD154 IL-10 IFN-γ CD25-PE Treg Detection Kit (APC) () Anti-FoxP3-APC gated on CD4 + cells Figure 13 AspergillusCD4 + T PBMCAspergillus lysate6 CD4 + T CD154CD4 + CD % CD154 () (# ) MS CD154 + CD4 + CD % Anti-IL-10 (# ) Anti-IFN-γ (# ) CD4-FITC CD25-PE Figure 11 PBMCT 7
8 MACS at a Glance & NK cells Human Hematopoietic stem cells CD133 Hematopoietic Tissue # Diamond CD133 # CD34 # (Large Scale # ) CD34 UltraPure # CD34 MultiSort Kit # CD34 + CD38 - Cell # Diamond CD34 # Lineage Cell # Leukocytes CD45 # Whole Blood CD45 # Tumor-Infiltrating Leukocytes CD45 (TIL) # Myeloid progenitor cells CD33 # Whole Blood CD33 # Antigen-presenting cells Anti-HLA-DR # Erythroblasts CD71 # Erythrocytes CD235a (Glycophorin A) # Megakaryocytes CD61 # Thrombocytes CD61 # Anti-CX3CR1 # Other CD123 # Granulocytes CD15 # Whole Blood CD15 # Basophils Basophil # Diamond Basophil # Eosinophils CD16 # Eosinophil # MACSxpress Eosinophil # Neutrophils CD16 # CD66abce # Whole Blood CD66b # MACSxpress Neutrophil # CD10 # Monocytes/ Macrophages CD14 # CD14 UltraPure # Pan Monocyte # Whole Blood CD14 # CD11b # Classical Monocyte # CD16 + Monocyte # Slan (M-DC8) + Monocyte # Myeloid dendritic cells Myeloid Dendritic Cell # MDC subsets MDC1s CD1c (BDCA-1) + Dendritic Cell # # MDC2s CD141 (BDCA-3) # Dendritic cells Blood Dendritic Cell # Pan-DC # Plasmacytoid dendritic cells Plasmacytoid Dendritic Cell # Diamond Plasmacytoid Dendritic Cell # CD304 (BDCA-4/ Neuropilin-1) # CD303 (BDCA-2) # Tissue dendritic cells CD209 (DC-SIGN) # CD1a # CD207 (Langerin) # CD19 # Whole Blood CD19 # REAlease CD19 # CD20 # CD22 # Pan B Cell # B Cell # B-CLL Cell # MACSxpress Pan B Cell # MACSxpress B Cell # MACSxpress B-CLL Cell # Naive/ resting Naive B Cell # CD43 # Anti-IgD # MACSxpress Naïve B Cell # Activated B cell subsets B Pan B Cell # CD25 # CD30 # CD69 # Memory Memory B Cell # IgM + Memory B Cell # IgG + Memory B Cell # Switched Memory B Cell # CD27 # Anti-IgG # Anti-IgM # Plasma cells CD138 # Whole Blood & Bone Marrow CD138 # CD38 # Plasma Cell #
9 Mouse Hematopoietic stem cells Anti-Sca-1 (FITC) # CD105 MultiSort Kit (PE) # CD117 # Lineage Cell # Direct Lineage Cell # Leukocytes CD45 # Tumor-Infiltrating Leukocytes CD45 (TIL) # Myeloid cells Antigen-presenting cells Anti-MHC Class # B cell Progenitors CD93 # Erythrocytes Anti-Ter-119 # Megakaryocytes CD61 # Myeloid-derived suppressor cells Myeloid-Derived Suppressor Cell # Neutrophils Anti-Ly-6G # Anti-Ly-6B.2 (7/4) # Neutrophil # Monocytes/ Macrophages Anti-F4/80 UltraPure # Macrophage (Peritoneum) # CD11b # CD115 # Monocyte (BM) # Dendritic cells CD11c UltraPure # Pan DC # Pan Dendritic Cell # CD45R (B220) # CD19 # CD22 # Pan B Cell # Other CD23 # Anti-Siglec-F # Indirect Conventional dendritic cells CD8 + DCs Anti-XCR1 (Spleen) # CD8 + Dendritic Cell # Plasmacytoid dendritic cells Anti-mPDCA-1 # Plasmacytoid Dendritic Cell # B-2 cells B-1a cells CD5 (Ly-1) # B-1a Cell # B-2 cell subsets 1 Anti- ( web ) Anti-FITC MultiSort Kit # Anti-PE MultiSort Kit # Anti-APC MultiSort Kit # Anti-Biotin MultiSort Kit # Anti-Biotin # Anti-Biotin UltraPure # Anti-FITC # Anti-PE # Anti-PE UltraPure # Anti-APC # Anti-Cy5/Anti-Alexa Fluor 647 # Anti-Cy7 # CD4 + DCs CD4 + Dendritic Cell # MACS () untouched 2 MACSxpress Tissue dendritic cells Epidermal Langerhans Cell # CD207 (Langerin) # MACSxpressMACS MACSxpress2 Resting B Cell # CD43 (Ly-48) # GC Germinal Center B Cell (PNA) # Memory Memory B Cell # Anti-Mouse IgG1 # Anti-Mouse IgG2a+b # Plasma cells CD138 + Plasma Cell # CD138 # Regulatory Regulatory B Cell # MZ and FO MZ and FO B Cell #
10 DC & Monocytes / Macrophages untouchedpositive (DC) PBMC DC PBMC32 pan-monocyte Pan-DC Pan Monocyte (PBMC) (Pan DC) (PBMC) (Pan monocyte) CD303 (BDCA-2)-FITC CD1c (BDCA-1)-PE CD303 (BDCA-2)-FITC CD1c (BDCA-1)-PE CD303 (BDCA-2)-FITC CD141 (BDCA-3)-APC CD14-FITC CD16-PE CD14-FITC CD16-PE CD14-FITC Anti-Slan (M-DC8)-APC Figure 14 pan DC CD1c + CD20 MDC1CD141 + MDC2CD303 + PDC3CD14 + (88.6%, n=13) Figure 17 (Pan-monocyte) Classical (CD14 ++ CD16 ), non classical (CD14 + CD16 ++ ), intermediate (CD14 ++ CD16 + ) monocytes Pan Dendritic Cell (Pan DC) () Anti-F4/80 UltraPure () () (F4/80 + ) CD11c-APC CD11c-APC CD11b-APC CD11b-APC Anti-mPDCA-1-FITC Anti-mPDCA-1-FITC Anti-F4/80-FITC Anti-F4/80-FITC Figure 15 pan DC CD11c hi conventional DCmPDCA-1 + CD11c low Plasmacytoid DC Myeloid Dendritic Cell Plasmacytoid Dendritic Cell MDCPDC 2p.8-9 MACS T (DC) Blood Dendritic Cell Enumeration Kit ( 3DC Figure 18 TAM) F4/80UltraPure MACS () in vitro Mo-DC CytoBox Mo-DC GM-CSFIL-4 Mo-DC Differentiation Inspector ( ) Mo-DC GM-CSF+IL-4 TNF-α or LPS CD1c (BDCA-1)-PE MDC1s PDCs CD303 (BDCA-2)-FITC CD141 (BDCA-3)-APC MDC2s CD303 (BDCA-2)-FITC DC PDC CD303 (BDCA-2) MDC1 CD1c (BDCA-1) MDC2 CD141 (BDCA-3) MFI Mo-DC Mo-DC CD14 CD209 CD83 381,6 1,4 0,5 MFI 804,2 301,1 7,8 370,5 3,2 2,9 Monocytes immo-dcs mmo-dcs Monocytes immo-dcs mmo-dcs Monocytes immo-dcs mmo-dcs MFI Figure 16DC Figure 19 10
11 & & BPlasma BPan Bresting B B Pan B Cell (PBMC) (B&) Eosinophil (PBMC) () CD19-APC CD19-APC CD43-PE CD43-PE Figure 20Pan-B BCD43 + BPlasma (>95%, n=5) MZ and FO B Cell () (MZ B) (FO B) Figure 22Eosinophil () Myeloid-Derived Suppressor Cell () (Gr-1 high Ly-6G + ) (Gr-1 dim Ly-6G ) CD21/CD35-PE-Cy7 CD21/CD35-PE-Cy7 CD21/CD35-PE-Cy7 Anti-Gr-1-PE Anti-Gr-1-PE Anti-Gr-1-PE Side scatter Side scatter CD16-FITC CD16-FITC CD23-PE CD23-PE CD23-PE CD11b-APC CD11b-APC CD11b-APC Figure 21BB CD21 hi CD23 lo/ marginal zone (MZ) BCD21 int CD23 hi follicular (FO) B 12 Figure 23Myeloid-Derived Suppressor Cell (MDSC) MDSC MACS Marker Screen () 1 MACS Marker Screen well plate 1 MACSQuant all-in-one solution 4 MACS Marker Screen Plate 1 A B C D E F G H Empty CD1a CD1b CD1c (BDCA-1) CD1d CD2 CD3 CD4 (VIT4) CD5 CD6 CD7 CD8 CD9 CD10 CD11a CD11b CD11c CD13 CD14 CD15 CD16 CD18 CD19 CD20 CD21 CD22 CD23 CD24 CD25 CD26 CD27 CD28 CD29 CD30 CD31 CD32 CD33 CD34 CD35 CD36 CD37 CD38 CD39 CD40 CD41a CD41b CD42a CD42b CD43 CD44 CD45 CD45RA CD45RB CD45RO CD46 CD47 CD48 CD49a CD49b CD49c CD49d CD49e CD49f CD51 CD51/ CD61 CD52 CD53 CD54 (ICAM-1) CD61 CD62E CD62L CD62P CD63 CD64 CD66acde CD66b CD66c CD68 CD69 CD70 CD71 CD72 CD73 CD74 CD79a CD79b CD80 CD81 CD82 CD83 CD84 CD55 (DAF) CD56 CD57 CD58 (LFA-3) CD85a (ILT5) doclogic gatelogic TM TM platelogic TM graphlogic TM 11
12 Research Translational research Clinical MACS Sample Preparation Tissue dissociation Automated cell processing CliniMACS Prodigy MACS Cell Separation Cell isolation or depletion Cell isolation & phenotyping MACSQuant Flow Sorting MACS Cell Culture MACS Flow Cytometry T / TLR 3 Vio Dyes REA 1 Culture or expansion Phenotyping of cells GMP compliant tools GCTP/MF IL-4/GM-CSF/TNF-α Texpansion kit CD3 T / MACS GMP Cytokine MACS GMP Cell Culture & Stimulation MACSmolecular KIR Molecular applications Cryopreservation CryoMACS DMSO 10 (EP, USP) CryoMACS Freezing Products Viscover Imaging Agents CT, MRI,, Imaging MACS GMPGMP GMP NEX5F TEL FAX [email protected] AM900PM dE200
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ミルテニーバイオテク株式会社 初めての MACS スターティングキットの選択方法 初めて MACS テクノロジーによる細胞分離をお試しになる場合は スターティングキットが最適です スターティングキットは 細胞分離に 必要な機器と試薬がセットなっており 個々にご購入いただくよりも安価でご利 いただけます 多様な実験系のために 13 種類のスター ティングキットをご 意しています 選択方法に従って 最適なキットをお選びください
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インターロイキン インターロイキンは リンパ球や単球 マクロファージなどの免疫反応への寄与が知られる白血球から産生されることから 細胞間 ( inter-) 白血球 ( leukocyte -leukin) より Interleukin と名づけられました 現在までに 30 種類以上のインターロイキンが同定され 発見された順番に番号が付与されており 中にはケモカイン ( IL-8) やインターフェロン
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